FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4584308
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT52848411.528108495327976TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC86510.18870896109074695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT69360.15129873472724784TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG68520.14946639710944376No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA63780.13912677769469242No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC60420.13179742722347626No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT47780.10422510878413929No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG47410.10341800769058274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA651250.087.478041
TCGGAAG652500.087.053963
CGGAAGA653250.086.597394
ATCGGAA657800.086.4322
GAAGAGC657100.086.0372546
GGAAGAG680050.083.2943345
AGAGCAC823000.068.7928
GAGCACA823900.068.705339
AAGAGCA829650.068.1203847
CGACGCG301.2905616E-463.442831
TCGGACG2050.048.66453
GATCGGC3650.048.2339321
GACGCGT502.8295623E-547.49657
TTGTGTT390950.047.4421581
GCCGTCT615900.046.1183650-51
ATGCCGT614500.046.11009248-49
CGTCTTC616550.046.05508452-53
TCGTATG619550.045.96757544-45
TCTCGTA608800.045.94836842-43
AATCTCG603800.045.9232440-41
Produced by FastQC (version 0.11.2)