##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5178575 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69327411498337 33.0 33.0 33.0 33.0 33.0 2 32.720850040793074 33.0 33.0 33.0 33.0 33.0 3 32.76027169636435 33.0 33.0 33.0 33.0 33.0 4 36.69199750896723 37.0 37.0 37.0 37.0 37.0 5 36.70390483868632 37.0 37.0 37.0 37.0 37.0 6 36.59331765978092 37.0 37.0 37.0 37.0 37.0 7 36.48141834384942 37.0 37.0 37.0 37.0 37.0 8 36.427332808736 37.0 37.0 37.0 37.0 37.0 9 36.50041353074929 37.0 37.0 37.0 37.0 37.0 10-11 36.419138276456366 37.0 37.0 37.0 37.0 37.0 12-13 36.41971353123205 37.0 37.0 37.0 37.0 37.0 14-15 38.32625403706618 40.0 37.0 40.0 37.0 40.0 16-17 38.351330916323505 40.0 37.0 40.0 37.0 40.0 18-19 38.3183848838725 40.0 37.0 40.0 37.0 40.0 20-21 38.34838821490467 40.0 37.0 40.0 37.0 40.0 22-23 38.16328710118131 40.0 37.0 40.0 37.0 40.0 24-25 38.221267433608666 40.0 37.0 40.0 37.0 40.0 26-27 38.174239631558876 40.0 37.0 40.0 37.0 40.0 28-29 37.98904466962436 40.0 37.0 40.0 35.0 40.0 30-31 38.02104604065791 40.0 37.0 40.0 37.0 40.0 32-33 37.88375064568921 40.0 37.0 40.0 33.0 40.0 34-35 37.7174323052191 40.0 37.0 40.0 33.0 40.0 36-37 37.38060537889284 40.0 37.0 40.0 33.0 40.0 38-39 37.602712522267225 40.0 37.0 40.0 33.0 40.0 40-41 37.40672829880807 40.0 37.0 40.0 33.0 40.0 42-43 37.220247655001614 40.0 37.0 40.0 33.0 40.0 44-45 37.28301192895729 40.0 37.0 40.0 33.0 40.0 46-47 37.3334880734565 40.0 37.0 40.0 33.0 40.0 48-49 37.03340890109731 38.5 37.0 40.0 33.0 40.0 50-51 36.984038659283684 37.0 37.0 40.0 33.0 40.0 52-53 36.91778607049236 37.0 37.0 40.0 33.0 40.0 54-55 36.63069280641876 37.0 37.0 40.0 33.0 40.0 56-57 36.548995331727355 37.0 37.0 40.0 33.0 40.0 58-59 36.30060122717157 37.0 37.0 40.0 33.0 40.0 60-61 36.02880792496005 37.0 37.0 40.0 33.0 40.0 62-63 35.83288114973714 37.0 37.0 40.0 33.0 40.0 64-65 35.564771003606204 37.0 35.0 40.0 30.0 40.0 66-67 34.96554689272628 37.0 33.0 37.0 27.0 40.0 68-69 34.84053586169941 37.0 33.0 37.0 27.0 40.0 70-71 34.344355252168796 37.0 33.0 37.0 27.0 40.0 72-73 33.44602482343116 37.0 33.0 37.0 24.5 40.0 74-75 32.62825593913384 37.0 33.0 37.0 22.0 38.5 76-77 30.103097666829193 33.0 30.0 35.0 18.5 37.0 78-79 32.15456375547327 35.0 33.0 37.0 18.5 37.0 80-81 32.407892229039845 37.0 33.0 37.0 15.0 37.0 82-83 32.30132198529518 37.0 33.0 37.0 15.0 37.0 84-85 32.101434371424574 37.0 33.0 37.0 15.0 37.0 86-87 31.925041734453977 37.0 33.0 37.0 15.0 37.0 88-89 31.786145513003095 37.0 33.0 37.0 15.0 37.0 90-91 31.629739069145472 37.0 33.0 37.0 15.0 37.0 92-93 31.496294154279894 33.0 33.0 37.0 15.0 37.0 94-95 31.339336497009313 33.0 33.0 37.0 15.0 37.0 96-97 31.19911906267651 33.0 33.0 37.0 15.0 37.0 98-99 31.066834409079718 33.0 33.0 37.0 15.0 37.0 100-101 29.67146396450761 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12713275618751396 2306 2 0.11714156473970405 2306 3 0.07746578806102633 2306 4 0.1009783758213132 2306 5 0.12436587345958827 2306 6 0.1618156266527535 2306 7 0.16413635101596213 2306 8 0.16737554629143148 2306 9 0.15745387738073902 2306 10-11 0.1480615937801275 2306 12-13 0.16126209920805223 2306 14-15 0.3667607756842486 2306 16-17 0.2927931987568897 2306 18-19 0.35519190649021226 2306 20-21 0.33324126070389326 2306 22-23 0.33838519345343343 2306 24-25 0.31187794515373213 2306 26-27 0.3123113162552045 2306 28-29 0.33518420428362816 2306 30-31 0.38318665717719824 2306 32-33 0.3685717260645802 2306 34-35 0.38483103911757865 2306 36-37 0.3934677629639509 2306 38-39 0.4264474026577787 2306 40-41 0.431542118607652 2306 42-43 0.45658122097032816 2306 44-45 0.4281941674845413 2306 46-47 0.41336993239685427 2306 48-49 0.35877144577585796 2306 50-51 0.29128837066428304 2306 52-53 0.5009532538300334 2306 54-55 0.4286283971360021 2306 56-57 0.33853494204883106 2306 58-59 0.3699271743829513 2306 60-61 0.39654247534657827 2306 62-63 0.38312343828904005 2306 64-65 0.25078290329153674 2306 66-67 0.1991166834012219 2306 68-69 0.2578280785926381 2306 70-71 0.3898821170835731 2306 72-73 0.15443587355915867 2306 74-75 0.13726924943139807 2306 76-77 -0.14582837327855813 2306 78-79 0.20280969691355466 2306 80-81 0.31251045708491887 2306 82-83 0.34761120496819586 2306 84-85 0.3633091794153671 2306 86-87 0.3395601921352629 2306 88-89 0.3005536147970638 2306 90-91 0.39613843280404026 2306 92-93 0.372314910199794 2306 94-95 0.21756199762339534 2306 96-97 0.3197656028709517 2306 98-99 0.32542393246223966 2306 100-101 0.24485535214809317 2307 1 0.10754880424661195 2307 2 0.13672143440776807 2307 3 0.11158893376020984 2307 4 0.16592053287995867 2307 5 0.12211188590264044 2307 6 0.15884724134922124 2307 7 0.1739426149413461 2307 8 0.18332412684219435 2307 9 0.16611631710454589 2307 10-11 0.20586213679402476 2307 12-13 0.1870907826893884 2307 14-15 0.3649028760207784 2307 16-17 0.3513513256668688 2307 18-19 0.2965375955026772 2307 20-21 0.389002028056602 2307 22-23 0.3720165327039169 2307 24-25 0.3440999692305482 2307 26-27 0.3524535583970163 2307 28-29 0.41859015910218034 2307 30-31 0.43588883532042644 2307 32-33 0.42503775976409486 2307 34-35 0.4387700026929693 2307 36-37 0.4835735153160883 2307 38-39 0.4439274084732219 2307 40-41 0.38347754564561853 2307 42-43 0.27036336797368676 2307 44-45 0.31451894738199115 2307 46-47 0.28814818262012665 2307 48-49 0.3976607882506187 2307 50-51 0.2984639320305078 2307 52-53 0.3446748972528155 2307 54-55 0.2861330827974129 2307 56-57 0.30200694374476456 2307 58-59 0.3450681023193809 2307 60-61 0.31453842315364966 2307 62-63 0.2916863636600837 2307 64-65 0.10353224242886228 2307 66-67 0.15309729332143007 2307 68-69 0.27186748040358566 2307 70-71 0.23911672673592932 2307 72-73 0.04780798132257047 2307 74-75 -0.05761519718070929 2307 76-77 -0.2501267201012958 2307 78-79 0.0041970967277862314 2307 80-81 0.20912034434972782 2307 82-83 0.2099207973783237 2307 84-85 0.3042736414398419 2307 86-87 0.17085801112728305 2307 88-89 0.25764412881251175 2307 90-91 0.35009497880015417 2307 92-93 0.2855810077833034 2307 94-95 0.2721184586795893 2307 96-97 0.29980357517022327 2307 98-99 0.18300320912646129 2307 100-101 0.08021696632174269 2201 1 0.10779387066018131 2201 2 0.10570581381340105 2201 3 0.1009413524529279 2201 4 0.08535447151968611 2201 5 0.0550707378754538 2201 6 0.0587154369091607 2201 7 0.16360987324650722 2201 8 0.10165086995352368 2201 9 0.15433095475702885 2201 10-11 0.15732809081826815 2201 12-13 0.13814373672413893 2201 14-15 0.26007944522480386 2201 16-17 0.2256628410164936 2201 18-19 0.1504342375002139 2201 20-21 0.15323944044583726 2201 22-23 0.2971665211865613 2201 24-25 0.24931403436048072 2201 26-27 0.24465281918342896 2201 28-29 0.27389172746988066 2201 30-31 0.212630274005555 2201 32-33 0.2551090526805879 2201 34-35 0.25321305485942247 2201 36-37 0.20640148997217267 2201 38-39 0.2913080939136634 2201 40-41 0.27588947131900454 2201 42-43 0.2081991753881951 2201 44-45 0.3328153917206649 2201 46-47 0.2632104767055594 2201 48-49 0.20279102996218512 2201 50-51 0.2553028046867709 2201 52-53 0.2474903891968765 2201 54-55 0.07416114882770586 2201 56-57 0.18533774421540272 2201 58-59 0.157363860395634 2201 60-61 0.15407860821511576 2201 62-63 0.2165121457172816 2201 64-65 0.0986138053867478 2201 66-67 0.15485118126567698 2201 68-69 0.08585509518289314 2201 70-71 0.11235430830127768 2201 72-73 -0.19897265460756586 2201 74-75 -0.1030725318253971 2201 76-77 -0.13103619941163203 2201 78-79 0.0633652472696653 2201 80-81 0.03398177764416488 2201 82-83 0.11410451307834535 2201 84-85 0.17925287674469814 2201 86-87 0.14520183242378337 2201 88-89 0.12336837939578871 2201 90-91 0.0912456822191352 2201 92-93 0.004213223694076618 2201 94-95 0.0023735514062543928 2201 96-97 0.09961251130098603 2201 98-99 0.0030549751052397767 2201 100-101 -0.03474689976341594 1101 1 0.13176310433557603 1101 2 0.11932689936236329 1101 3 0.0616730592512198 1101 4 0.11659109332904904 1101 5 0.11553045871057321 1101 6 0.08127349153780017 1101 7 0.07524688635509591 1101 8 0.026493571031160457 1101 9 0.09699269436919877 1101 10-11 0.08778294197044545 1101 12-13 0.07681056573273537 1101 14-15 0.12942805341584318 1101 16-17 0.12507551850683996 1101 18-19 0.0940139218336924 1101 20-21 0.11456455182740655 1101 22-23 0.13758736733866783 1101 24-25 0.20259111943529717 1101 26-27 0.21503585127877756 1101 28-29 0.15561772913365246 1101 30-31 0.22594636483263741 1101 32-33 0.19125506912644852 1101 34-35 0.19123143594364223 1101 36-37 0.0603935808620335 1101 38-39 0.12586445291996284 1101 40-41 0.10639831955764123 1101 42-43 0.14436502468279855 1101 44-45 -0.05437720415377356 1101 46-47 0.09178823782757917 1101 48-49 0.04924721319196124 1101 50-51 0.17310927091979522 1101 52-53 0.18545947559181286 1101 54-55 0.11982398361553948 1101 56-57 0.0040232540429201435 1101 58-59 -0.011353982724003231 1101 60-61 0.09294359325166113 1101 62-63 0.09990689477224635 1101 64-65 0.09407731429597987 1101 66-67 0.1704397716404813 1101 68-69 0.10509226863288035 1101 70-71 0.025678320770566643 1101 72-73 -0.2307609170447904 1101 74-75 -0.32934915361636286 1101 76-77 -0.2887274960045261 1101 78-79 -0.16714997047517244 1101 80-81 -0.03438912284132556 1101 82-83 0.10058497746363315 1101 84-85 0.08839715945465798 1101 86-87 0.09146245801621333 1101 88-89 -0.030164428706225266 1101 90-91 -0.07692215863840701 1101 92-93 -0.03496679895906141 1101 94-95 -0.05586796589750165 1101 96-97 -0.018889737255431527 1101 98-99 -0.0274558219263632 1101 100-101 -0.15090424450253082 2304 1 0.06444145458991812 2304 2 0.09694327695446958 2304 3 0.08771887372677867 2304 4 0.08994216575138125 2304 5 0.09656851008459455 2304 6 0.09871953108903142 2304 7 0.059258755247086015 2304 8 0.053081590156935476 2304 9 0.07366569194932282 2304 10-11 0.009978028574423092 2304 12-13 0.024276402867478453 2304 14-15 0.1032769343022224 2304 16-17 0.14255889239474584 2304 18-19 0.07477456195088905 2304 20-21 0.051086573296366566 2304 22-23 0.04297509636781882 2304 24-25 0.09058943352258098 2304 26-27 0.030761951422761058 2304 28-29 0.12270691421750968 2304 30-31 0.02536958827511171 2304 32-33 0.10258420214785957 2304 34-35 0.04380997941745335 2304 36-37 0.025106142038559653 2304 38-39 0.10980700145109523 2304 40-41 0.13399714378046923 2304 42-43 0.09942359623369157 2304 44-45 0.15028892847783482 2304 46-47 0.05511566395842493 2304 48-49 0.12732489052473284 2304 50-51 0.05528846524688902 2304 52-53 0.06085295950904879 2304 54-55 0.05455154239491833 2304 56-57 0.029432878959532616 2304 58-59 -0.018972472120665884 2304 60-61 0.05654139475251441 2304 62-63 0.1279756695013745 2304 64-65 0.10298477296429809 2304 66-67 0.004052731712839375 2304 68-69 -0.004580253155140213 2304 70-71 0.005315321974862286 2304 72-73 -0.01810375649296958 2304 74-75 -0.040219458544427766 2304 76-77 -0.39669808610149815 2304 78-79 0.06881627824596848 2304 80-81 0.23326704567642054 2304 82-83 0.24897691904178743 2304 84-85 0.2866943865727194 2304 86-87 0.2476387905288 2304 88-89 0.15295610496604795 2304 90-91 0.11613831763868632 2304 92-93 0.13055851359138515 2304 94-95 0.11113662238343025 2304 96-97 0.1537801678577182 2304 98-99 0.1328701260451517 2304 100-101 0.15439410020513478 2202 1 0.05207919625127033 2202 2 0.07428802049346928 2202 3 0.048398995128245303 2202 4 0.08620697728770921 2202 5 0.052072811113475836 2202 6 0.06397017045522091 2202 7 0.08816109040850506 2202 8 0.07769996930763057 2202 9 0.06959017018103708 2202 10-11 0.07137964159315402 2202 12-13 0.06890051138368136 2202 14-15 -0.015648688818970413 2202 16-17 -0.04535216510738849 2202 18-19 -0.01842179776614472 2202 20-21 -0.01762286136018787 2202 22-23 -0.02199172581889286 2202 24-25 -0.0536254459750225 2202 26-27 0.047039026386812566 2202 28-29 0.06033249877602742 2202 30-31 -0.002739596044875725 2202 32-33 0.05482791245723462 2202 34-35 -0.04002660551348214 2202 36-37 -0.005167573340223441 2202 38-39 0.03707975086596349 2202 40-41 0.09037604894237461 2202 42-43 -0.13289732748692273 2202 44-45 -0.06999381702176066 2202 46-47 -0.1067844160381739 2202 48-49 -0.1625201369766387 2202 50-51 0.027833108699972797 2202 52-53 -0.057113655320726764 2202 54-55 -0.014988397329645409 2202 56-57 -0.02437851915225764 2202 58-59 -0.10757517533011907 2202 60-61 -0.20174057593375494 2202 62-63 -0.24504007749487045 2202 64-65 -0.24080708806729945 2202 66-67 -0.09662363037799793 2202 68-69 -0.10254432368197541 2202 70-71 -0.15445938449702368 2202 72-73 -0.3412929699600298 2202 74-75 -0.24753469013665352 2202 76-77 -0.498322519540924 2202 78-79 -0.2871211866100296 2202 80-81 -0.030403020435649353 2202 82-83 -0.11106007264974238 2202 84-85 0.09323963396987978 2202 86-87 -0.015342271604321667 2202 88-89 -0.02268787136162942 2202 90-91 -0.02221342371699464 2202 92-93 -0.016684212861527214 2202 94-95 -0.0693326066567117 2202 96-97 -0.08140487654978301 2202 98-99 -0.04749418328644239 2202 100-101 -0.10115445445795856 1102 1 0.08229355132831984 1102 2 0.1065261036684717 1102 3 0.05792641754749894 1102 4 0.025822219001881308 1102 5 0.03449839692728318 1102 6 -0.008418387703954977 1102 7 0.032747284202045535 1102 8 0.03341751205620369 1102 9 -0.017567988991437744 1102 10-11 -0.014780924165762599 1102 12-13 0.005656411742563705 1102 14-15 -0.044286877546014125 1102 16-17 -0.09425710494273432 1102 18-19 -0.04108263796036482 1102 20-21 -0.0989160004777645 1102 22-23 -0.10242419362552369 1102 24-25 -0.0380404976955262 1102 26-27 -0.09275759594799382 1102 28-29 -0.11576963337512325 1102 30-31 -0.05317043835013635 1102 32-33 -0.04428338161074663 1102 34-35 -0.09368525022532737 1102 36-37 -0.10775294382787592 1102 38-39 -0.1171190247304068 1102 40-41 -0.04207731816761395 1102 42-43 -0.1316034283946621 1102 44-45 -0.16197352992021052 1102 46-47 -0.2644916357975191 1102 48-49 -0.1600224802318735 1102 50-51 -0.1619812041562625 1102 52-53 -0.2318942337458907 1102 54-55 -0.25546017271491195 1102 56-57 -0.41148708997767613 1102 58-59 -0.22747249430837257 1102 60-61 -0.2643833667296178 1102 62-63 -0.3053573458081402 1102 64-65 -0.23581107793430078 1102 66-67 -0.26252086491462734 1102 68-69 -0.1043011317883753 1102 70-71 -0.29356200218754225 1102 72-73 -0.3394659486385194 1102 74-75 -0.3665596848590482 1102 76-77 -0.4468456565474703 1102 78-79 -0.03799576967948326 1102 80-81 0.06024485796840651 1102 82-83 0.08017592059492529 1102 84-85 0.0628980253568372 1102 86-87 0.03412882824790486 1102 88-89 0.026545166426000577 1102 90-91 0.008041801108841895 1102 92-93 -0.04612755252798095 1102 94-95 0.01177254428269947 1102 96-97 0.03549514456062397 1102 98-99 0.02189737255058688 1102 100-101 0.008126693948202757 2305 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-0.23818881049624707 1213 64-65 -0.13121000621130463 1213 66-67 -0.11590245326307524 1213 68-69 -0.0618075896064596 1213 70-71 -0.20666513491632088 1213 72-73 0.216025614977859 1213 74-75 0.22482690486340573 1213 76-77 0.16452908926181564 1213 78-79 -0.4697307792975103 1213 80-81 -0.468678747417691 1213 82-83 -0.4946496633567108 1213 84-85 -0.44470697601716935 1213 86-87 -0.5354634223703947 1213 88-89 -0.6371383455278341 1213 90-91 -0.5298100812764268 1213 92-93 -0.42307569762278874 1213 94-95 -0.33199489182612396 1213 96-97 -0.467034066761169 1213 98-99 -0.4285468703732924 1213 100-101 -0.14614524889323732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 19.0 10 38.0 11 27.0 12 21.0 13 70.0 14 190.0 15 576.0 16 1323.0 17 2501.0 18 3901.0 19 5803.0 20 8182.0 21 11201.0 22 15512.0 23 20843.0 24 28079.0 25 37166.0 26 52424.0 27 79862.0 28 142622.0 29 212394.0 30 145825.0 31 97130.0 32 122719.0 33 176095.0 34 272536.0 35 480156.0 36 978752.0 37 1676703.0 38 605453.0 39 449.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.923395552849584 24.29866450876586 23.0194545665886 12.758485371795954 2 26.814944265555678 29.788851180102633 32.07129374393535 11.324910810406337 3 34.47299306722284 18.15837243883128 32.68949269065137 14.679141803294518 4 25.40184462387675 18.438398179438618 33.60412329962074 22.555633897063892 5 36.79913875921465 17.44968451745895 31.740295351520448 14.01088137180595 6 30.498602414756952 17.96258623269915 37.326658395408 14.212152957135892 7 29.896873174570228 26.540177558498236 28.39638317490816 15.166566092023384 8 25.37219215710886 33.9777641532661 26.227639843007005 14.422403846618037 9 36.489401041792384 23.252284653596792 24.446184519872745 15.81212978473808 10-11 26.286275664637472 30.940220041227555 28.056453754169823 14.717050539965145 12-13 22.385501944986554 29.232796965880837 27.483771824676328 20.897929264456284 14-15 21.308999097242 30.083295114968887 28.048082725460187 20.55962306232892 16-17 24.63252921894537 26.56281892219385 28.738475352775616 20.066176506085167 18-19 19.056709616062335 26.893701452619688 33.379404565927885 20.670184365390092 20-21 23.565729954668996 26.99516758953959 33.04589583041667 16.39320662537474 22-23 18.89748468085045 36.05967287285528 28.636316896516266 16.406525549778003 24-25 18.91793174317213 27.013346436040734 33.06391229167646 21.00480952911067 26-27 19.01449723138122 27.12697025726189 28.2869998020691 25.571532709287787 28-29 22.994124831638047 31.660794716693296 28.23921638674732 17.105864064921334 30-31 18.052118584745806 27.802262205336408 32.440304523927914 21.705314685989872 32-33 17.847612518887917 32.29984117252333 28.424692120901984 21.42785418768677 34-35 22.608651994033107 32.2611336130113 28.642425763844297 16.487788629111293 36-37 18.03212273646708 32.301569447193486 33.271150847482176 16.395156968857265 38-39 18.399440382000112 27.815563579064147 32.66436968388245 21.12062635505329 40-41 22.764254645341627 27.649179552289965 32.402987308284615 17.183578494083797 42-43 18.300488068628916 32.19333503907929 32.638669904365585 16.867506987926216 44-45 17.923023277392165 27.871647080644244 32.88662661503461 21.318703026928983 46-47 22.506195546081376 27.77149487831317 28.35500153180876 21.367308043796697 48-49 18.490546763329018 32.31640506268493 32.122871800338245 17.070176373647804 50-51 22.912990844729226 27.738321193184113 31.803476921229063 17.545211040857602 52-53 18.231248141954797 27.59617251619607 27.68605386875546 26.48652547309367 54-55 22.766744402214986 27.57742681720965 32.01466769670372 17.641161083871644 56-57 18.35715538869018 27.523861562720782 31.931473664584313 22.187509384004724 58-59 18.44213331958361 27.567989913265713 32.327247632976665 21.662629134174015 60-61 22.560198945794298 27.990617352992064 32.081727976617145 17.367455724596486 62-63 18.147634238991746 28.08510786207951 32.02551803024581 21.741739868682924 64-65 22.653992624520832 27.86334720540325 32.051510559695636 17.43114961038028 66-67 18.49325676809597 36.24096806681144 27.56108111866099 17.704694046431595 68-69 18.343156585602305 36.5026775765277 27.517028150763107 17.637137687106886 70-71 17.985715434782964 36.98385693689382 27.5817714448322 17.448656183491014 72-73 17.9270603002945 36.780756046927046 27.521749625838844 17.7704340269396 74-75 18.408616183955708 35.77578215706253 27.51909188592917 18.296509773052595 76-77 18.900988445127634 34.7646589106344 27.87945353091293 18.454899113325034 78-79 18.915094262195755 34.15212398315795 28.320660534917646 18.612121219728646 80-81 18.473066192653928 34.21580549900477 28.147010248658756 19.16411805968254 82-83 18.046151692309177 33.909424890051795 28.26263981886909 19.78178359876993 84-85 18.138293404241143 33.26976347649103 28.877534370109036 19.71440874915879 86-87 18.05217123978554 33.07438469928915 29.463274580528026 19.410169480397286 88-89 18.32693969575104 33.32791008675585 29.019605760549982 19.32554445694312 90-91 18.7063605542282 32.80747777038786 28.76197224224194 19.724189433141994 92-93 18.33826916113458 33.248975256487626 28.697491852745927 19.715263729631864 94-95 18.08345665575095 33.44686463012641 28.538490788525117 19.931187925597524 96-97 18.54463233279816 33.58195674410354 28.197714755499923 19.67569616759837 98-99 18.820732276189208 32.77298379850977 28.099609584147682 20.306674341153332 100-101 18.69014031603818 32.874441879312 27.868700023087158 20.56671778156266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 308.5 2 235.5 3 558.5 4 666.0 5 424.5 6 355.5 7 357.0 8 343.0 9 355.0 10 345.5 11 341.0 12 351.5 13 371.5 14 423.0 15 476.5 16 565.5 17 739.5 18 1059.5 19 1639.0 20 2617.5 21 4090.0 22 6353.5 23 9762.0 24 14089.5 25 19817.5 26 27841.5 27 37824.0 28 49016.0 29 60981.5 30 73508.0 31 86758.5 32 102728.5 33 123571.0 34 151024.5 35 189801.0 36 246743.0 37 318123.5 38 382115.0 39 403014.0 40 400853.5 41 371516.5 42 318628.0 43 293779.0 44 266555.0 45 236388.0 46 229629.5 47 201889.5 48 142758.5 49 97067.5 50 71420.0 51 55075.5 52 43291.0 53 32447.5 54 24188.5 55 18423.0 56 13934.0 57 10522.5 58 8099.5 59 6191.5 60 4545.5 61 3303.5 62 2493.0 63 1876.0 64 1316.0 65 845.0 66 524.5 67 338.5 68 237.0 69 162.0 70 111.5 71 77.5 72 42.5 73 30.5 74 27.5 75 21.5 76 16.5 77 9.5 78 5.0 79 4.0 80 2.0 81 2.0 82 1.5 83 1.0 84 1.5 85 0.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1567612711991233 2 0.0 3 0.0053103411652819545 4 0.00756964995196555 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.68964917955229E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.448274863258715E-4 24-25 2.8965497265174297E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.206890891799384E-4 40-41 0.0 42-43 0.0 44-45 1.834481493461039E-4 46-47 0.0010910337303215653 48-49 0.002645515416885919 50-51 5.406892822832536E-4 52-53 0.0015737920180744704 54-55 0.0023268949469690023 56-57 0.004161376440430041 58-59 0.005571030640668524 60-61 0.0015255161892991796 62-63 7.820684261597062E-4 64-65 0.0012358612166474367 66-67 0.008110339234248804 68-69 0.007434477631394738 70-71 0.009046890312489439 72-73 0.0062758577407877655 74-75 0.008863442163143337 76-77 0.004634479562427888 78-79 0.004373790087041319 80-81 0.0017862056646857486 82-83 0.0 84-85 3.8620663020232397E-4 86-87 0.0013613783714631921 88-89 0.004827582877529051 90-91 0.006700685034010321 92-93 0.00868964917955229 94-95 0.007994477245188107 96-97 0.010224820534606528 98-99 0.007308960476578982 100-101 0.007173788156008169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5178575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.2684164099753 #Duplication Level Percentage of deduplicated Percentage of total 1 93.28694760487896 48.75961023027363 2 3.540985813884194 3.701634420438288 3 0.9921651667084391 1.555767062429678 4 0.426321073727765 0.8913250962380243 5 0.30606798367529864 0.7998844410251017 6 0.17674675331785786 0.5542963740917388 7 0.16006679511974342 0.5856506530510267 8 0.10329971007957996 0.4319449809175367 9 0.0962864552555072 0.45294664841417753 >10 0.6944495117831128 7.263878307194835 >50 0.09554193705255495 3.498413944228291 >100 0.09618579114491263 10.375662519986411 >500 0.0166269642155837 5.991763584453763 >1k 0.008046333902131617 6.273627795339505 >5k 2.2466191022107646E-4 0.7766237103160232 >10k+ 3.744334399613383E-5 8.08697023160191 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 413211 7.9792413936266255 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8195 0.15824816672540226 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7200 0.13903438687283665 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6605 0.1275447396243175 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6278 0.12123026122050949 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5938 0.11466474850707 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5466 0.10555027203429515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001158619890606972 0.0 0.0 2 0.0012937922111777853 0.0 0.0 3 0.0013710335372182502 0.0 0.0 4 0.0015834471838295284 0.0 0.0 5 0.0017958608304408066 0.0 0.0 6 0.0018924124879913875 0.0 0.0 7 0.002317239781213944 0.0 1.93103315101162E-5 8 0.0027420670744365003 0.0 1.93103315101162E-5 9 0.002993101384068011 0.0 1.93103315101162E-5 10-11 0.005744823624259569 0.0 2.89654972651743E-5 12-13 0.008033097908208338 0.0 3.86206630202324E-5 14-15 0.010282751529136876 0.0 5.79309945303486E-5 16-17 0.011479992082764082 0.0 9.655165755058099E-5 18-19 0.01321792191867454 0.0 1.158619890606972E-4 20-21 0.014733782942218662 0.0 1.737929835910458E-4 22-23 0.016220678468497608 0.0 1.737929835910458E-4 24-25 0.01808412545922382 0.0 1.834481493461039E-4 26-27 0.020082744770520848 0.0 2.0275848085622012E-4 28-29 0.02215860540785834 0.0 2.220688123663363E-4 30-31 0.03919031779978083 0.0 2.317239781213944E-4 32-33 0.04231859150441965 0.0 2.317239781213944E-4 34-35 0.044655141617143713 0.0 2.510343096315106E-4 36-37 0.0468468642435419 0.0 2.510343096315106E-4 38-39 0.0492896211795716 0.0 2.606894753865687E-4 40-41 0.052350308723925015 0.0 2.703446411416268E-4 42-43 0.055555823754604314 0.0 2.799998068966849E-4 44-45 0.059456510719647776 0.0 2.89654972651743E-4 46-47 0.06332823218742607 0.0 2.89654972651743E-4 48-49 0.06743167763332578 0.0 2.89654972651743E-4 50-51 0.07253926031775151 0.0 2.89654972651743E-4 52-53 0.07806201512964474 0.0 3.089653041618592E-4 54-55 0.08464683817459437 0.0 3.089653041618592E-4 56-57 0.09190752282239806 0.0 3.089653041618592E-4 58-59 0.09951579343738384 0.0 3.282756356719754E-4 60-61 0.10834061493750693 0.0 3.475859671820916E-4 62-63 0.11821784950493137 0.0 3.475859671820916E-4 64-65 0.12845232520529298 0.0 3.475859671820916E-4 66-67 0.140588868559401 0.0 3.475859671820916E-4 68-69 0.1544150659206442 0.0 3.475859671820916E-4 70-71 0.1700564344438383 0.0 3.475859671820916E-4 72-73 0.18742607763718783 0.0 3.475859671820916E-4 74-75 0.20671709881579392 0.0 3.475859671820916E-4 76-77 0.2287984628976118 0.0 3.668962986922078E-4 78-79 0.2532839632524391 0.0 3.668962986922078E-4 80-81 0.2825873913190404 0.0 3.958617959573821E-4 82-83 0.31571426502464484 0.0 4.248272932225564E-4 84-85 0.3522011364130094 0.0 4.248272932225564E-4 86-87 0.39248248794311175 0.0 4.248272932225564E-4 88-89 0.4392328005291031 0.0 4.248272932225564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 49785 0.0 88.277084 1 TCGGAAG 49815 0.0 88.03721 3 ATCGGAA 49930 0.0 87.85347 2 CGGAAGA 49875 0.0 87.65784 4 GAAGAGC 51080 0.0 85.546974 6 GGAAGAG 53765 0.0 81.46915 5 AGAGCAC 68465 0.0 63.796673 8 GAGCACA 68655 0.0 63.59937 9 AAGAGCA 70245 0.0 62.180073 7 TCGGACG 130 0.0 62.120674 3 CGACGGG 95 0.0 55.085545 1 GCCGTCT 46555 0.0 46.407753 50-51 ATGCCGT 46410 0.0 46.398777 48-49 TATGCCG 46415 0.0 46.35796 48-49 TCGTATG 46795 0.0 46.29065 44-45 TGCCGTC 46545 0.0 46.27485 50-51 CGTATGC 46660 0.0 46.205723 46-47 TCTCGTA 46545 0.0 46.19742 42-43 CGTATCT 46235 0.0 46.178425 38-39 CTCGTAT 46530 0.0 46.151066 44-45 >>END_MODULE