FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178575
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4132117.9792413936266255TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81950.15824816672540226No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC72000.13903438687283665No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66050.1275447396243175No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA62780.12123026122050949No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59380.11466474850707No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54660.10555027203429515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA497850.088.2770841
TCGGAAG498150.088.037213
ATCGGAA499300.087.853472
CGGAAGA498750.087.657844
GAAGAGC510800.085.5469746
GGAAGAG537650.081.469155
AGAGCAC684650.063.7966738
GAGCACA686550.063.599379
AAGAGCA702450.062.1800737
TCGGACG1300.062.1206743
CGACGGG950.055.0855451
GCCGTCT465550.046.40775350-51
ATGCCGT464100.046.39877748-49
TATGCCG464150.046.3579648-49
TCGTATG467950.046.2906544-45
TGCCGTC465450.046.2748550-51
CGTATGC466600.046.20572346-47
TCTCGTA465450.046.1974242-43
CGTATCT462350.046.17842538-39
CTCGTAT465300.046.15106644-45
Produced by FastQC (version 0.11.2)