##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6433260 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66824051880384 33.0 33.0 33.0 33.0 33.0 2 32.70504814044512 33.0 33.0 33.0 33.0 33.0 3 32.73513350929389 33.0 33.0 33.0 33.0 33.0 4 36.67204092481883 37.0 37.0 37.0 37.0 37.0 5 36.67329596503173 37.0 37.0 37.0 37.0 37.0 6 36.54861811896301 37.0 37.0 37.0 37.0 37.0 7 36.47504779847232 37.0 37.0 37.0 37.0 37.0 8 36.41089929522513 37.0 37.0 37.0 37.0 37.0 9 36.43357613402847 37.0 37.0 37.0 37.0 37.0 10-11 36.378235995436214 37.0 37.0 37.0 37.0 37.0 12-13 36.36699363930573 37.0 37.0 37.0 37.0 37.0 14-15 38.21910990695231 40.0 37.0 40.0 37.0 40.0 16-17 38.19743613657772 40.0 37.0 40.0 37.0 40.0 18-19 38.19929110590898 40.0 37.0 40.0 37.0 40.0 20-21 38.15227326425483 40.0 37.0 40.0 37.0 40.0 22-23 38.02121599313567 40.0 37.0 40.0 35.0 40.0 24-25 38.02884898169824 40.0 37.0 40.0 35.0 40.0 26-27 37.94128000422803 40.0 37.0 40.0 33.0 40.0 28-29 37.805752604433835 40.0 37.0 40.0 33.0 40.0 30-31 37.77212952686507 40.0 37.0 40.0 33.0 40.0 32-33 37.62537306124733 40.0 37.0 40.0 33.0 40.0 34-35 37.521095136835754 40.0 37.0 40.0 33.0 40.0 36-37 37.37203828230166 40.0 37.0 40.0 33.0 40.0 38-39 37.31417539474543 40.0 37.0 40.0 33.0 40.0 40-41 37.2851082499386 40.0 37.0 40.0 33.0 40.0 42-43 37.0827958919739 38.5 37.0 40.0 33.0 40.0 44-45 36.95186235283511 37.0 37.0 40.0 33.0 40.0 46-47 36.930676670925784 37.0 37.0 40.0 33.0 40.0 48-49 36.692830229152875 37.0 37.0 40.0 33.0 40.0 50-51 36.55439808122165 37.0 37.0 40.0 33.0 40.0 52-53 36.40119449548129 37.0 37.0 40.0 33.0 40.0 54-55 36.193487827322386 37.0 37.0 40.0 33.0 40.0 56-57 36.02354809225805 37.0 37.0 40.0 33.0 40.0 58-59 35.800476818906745 37.0 37.0 40.0 27.0 40.0 60-61 35.46658094030087 37.0 33.0 40.0 27.0 40.0 62-63 35.199657016815735 37.0 33.0 38.5 27.0 40.0 64-65 34.910386025125675 37.0 33.0 37.0 27.0 40.0 66-67 34.49987875509461 37.0 33.0 37.0 27.0 40.0 68-69 34.228771027441766 37.0 33.0 37.0 27.0 40.0 70-71 33.812211538162614 37.0 33.0 37.0 27.0 37.0 72-73 33.30203458277763 37.0 33.0 37.0 27.0 37.0 74-75 32.81506887643279 37.0 33.0 37.0 22.0 37.0 76-77 30.124874946761054 33.0 30.0 33.0 22.0 37.0 78-79 32.57041569903906 33.0 33.0 37.0 27.0 37.0 80-81 33.0020702567594 37.0 33.0 37.0 27.0 37.0 82-83 32.95570651582557 37.0 33.0 37.0 27.0 37.0 84-85 32.79038232560164 37.0 33.0 37.0 27.0 37.0 86-87 32.62879177897365 37.0 33.0 37.0 27.0 37.0 88-89 32.50741373735866 33.0 33.0 37.0 22.0 37.0 90-91 32.366399150663895 33.0 33.0 37.0 22.0 37.0 92-93 32.23613269166799 33.0 33.0 37.0 22.0 37.0 94-95 32.058647559713116 33.0 33.0 37.0 22.0 37.0 96-97 31.892671911286037 33.0 33.0 37.0 22.0 37.0 98-99 31.749760385869685 33.0 33.0 37.0 22.0 37.0 100-101 30.11458032164097 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11132988619118578 2306 2 0.11297577020076233 2306 3 0.11228846836364426 2306 4 0.15099866282157848 2306 5 0.1593901593323679 2306 6 0.18103348242578932 2306 7 0.19407372400002743 2306 8 0.19800568213988612 2306 9 0.21268944125197464 2306 10-11 0.20440921709226245 2306 12-13 0.21336520797266445 2306 14-15 0.40797951264147514 2306 16-17 0.40956224870416946 2306 18-19 0.40886431655822975 2306 20-21 0.3692322853776062 2306 22-23 0.46836040233880993 2306 24-25 0.4358771584032013 2306 26-27 0.45550124193480457 2306 28-29 0.440553633714714 2306 30-31 0.46034137592157975 2306 32-33 0.4866922493330108 2306 34-35 0.5298256672600701 2306 36-37 0.48363264088119706 2306 38-39 0.5401478566644542 2306 40-41 0.5365997027074769 2306 42-43 0.5398524429624132 2306 44-45 0.5181719172679564 2306 46-47 0.447235637688145 2306 48-49 0.49681876738796404 2306 50-51 0.42348354239215524 2306 52-53 0.5192699185453762 2306 54-55 0.475248999644613 2306 56-57 0.4970953199551502 2306 58-59 0.5757143272086083 2306 60-61 0.5823703944224832 2306 62-63 0.5094044078422755 2306 64-65 0.4626879741027494 2306 66-67 0.4655078912244548 2306 68-69 0.4622190943345643 2306 70-71 0.48470960867855695 2306 72-73 0.4313656423411132 2306 74-75 0.5223826145659913 2306 76-77 0.031834546301801936 2306 78-79 0.28787739027529113 2306 80-81 0.4948903084189382 2306 82-83 0.5984978996465671 2306 84-85 0.6030708969979699 2306 86-87 0.5857314915648573 2306 88-89 0.5869833328452359 2306 90-91 0.5035892481892219 2306 92-93 0.4912174824872082 2306 94-95 0.3234141369919854 2306 96-97 0.5483760541920795 2306 98-99 0.572058450974481 2306 100-101 0.43200847890217986 2307 1 0.15608133369370591 2307 2 0.12999210424511176 2307 3 0.13058234125968937 2307 4 0.1769433358617718 2307 5 0.14985815932173807 2307 6 0.1949794021908957 2307 7 0.17334957871052836 2307 8 0.22629814715765662 2307 9 0.18134050791899625 2307 10-11 0.1917274436260712 2307 12-13 0.2381242305686655 2307 14-15 0.3754758313496964 2307 16-17 0.3527515711428322 2307 18-19 0.3375628083575606 2307 20-21 0.3845339299388115 2307 22-23 0.4056376543020903 2307 24-25 0.4086665238751195 2307 26-27 0.4445597034160542 2307 28-29 0.4813235306208341 2307 30-31 0.5269973064095907 2307 32-33 0.5409415624389382 2307 34-35 0.4276057796955328 2307 36-37 0.47084965956170777 2307 38-39 0.42896779788775774 2307 40-41 0.43951111086023786 2307 42-43 0.3465750617222625 2307 44-45 0.32976271789025446 2307 46-47 0.29482435836951737 2307 48-49 0.3408210509842391 2307 50-51 0.36336235860559896 2307 52-53 0.34219967338860613 2307 54-55 0.28952886990730065 2307 56-57 0.33623506648393686 2307 58-59 0.3038180347099413 2307 60-61 0.3330250799564851 2307 62-63 0.28857661968987713 2307 64-65 0.2108706157605411 2307 66-67 0.25029968462229846 2307 68-69 0.22717901850393218 2307 70-71 0.29991978542483366 2307 72-73 0.1146757988104028 2307 74-75 0.07027460462406765 2307 76-77 -0.279276203247278 2307 78-79 0.11223870939524971 2307 80-81 0.27726287551231366 2307 82-83 0.2836691782214089 2307 84-85 0.3096256107780775 2307 86-87 0.15213796638818167 2307 88-89 0.23652265116650995 2307 90-91 0.2569321843149268 2307 92-93 0.20917036549429469 2307 94-95 0.19489754040932183 2307 96-97 0.3033205625370776 2307 98-99 0.12924664356868476 2307 100-101 -0.05022451786855342 2201 1 0.11411407559207731 2201 2 0.11218459135818648 2201 3 0.09612237179069894 2201 4 0.11717820062852269 2201 5 0.0767668939467896 2201 6 0.11758019766629957 2201 7 0.1522019626421809 2201 8 0.1333399053756068 2201 9 0.1549792713362379 2201 10-11 0.18810059708984994 2201 12-13 0.13408487018055126 2201 14-15 0.24431649828252944 2201 16-17 0.19479960235693738 2201 18-19 0.22693026203317146 2201 20-21 0.16844944936993045 2201 22-23 0.3281683317398816 2201 24-25 0.16998892458134662 2201 26-27 0.21717634413453624 2201 28-29 0.23263211198162992 2201 30-31 0.24636087034578935 2201 32-33 0.2731328251740166 2201 34-35 0.30582657570209903 2201 36-37 0.17815302057287852 2201 38-39 0.20641035932953145 2201 40-41 0.17287875199509273 2201 42-43 0.1784533495504661 2201 44-45 0.2839479863095491 2201 46-47 0.22861489369265797 2201 48-49 0.10229282580635157 2201 50-51 0.2609122806774238 2201 52-53 0.1878959926853696 2201 54-55 0.25232288935233527 2201 56-57 0.2010783283814206 2201 58-59 0.2456154646150992 2201 60-61 0.1795493575008038 2201 62-63 0.13773991958249354 2201 64-65 0.14757353821744346 2201 66-67 0.24506725049473488 2201 68-69 0.1270514960513367 2201 70-71 0.17330263592255335 2201 72-73 0.12836245352421116 2201 74-75 0.025513495105862205 2201 76-77 0.026933826037055297 2201 78-79 0.1139304414354072 2201 80-81 0.2601924580893993 2201 82-83 0.24643536546412292 2201 84-85 0.305971190851146 2201 86-87 0.1654171520856309 2201 88-89 0.13851632875724107 2201 90-91 0.07308390413997756 2201 92-93 0.04331060706427081 2201 94-95 0.11500432767211066 2201 96-97 0.10517337892302336 2201 98-99 0.034798423350430596 2201 100-101 0.002213202800696479 1101 1 0.10957535591636258 1101 2 0.1239660925275956 1101 3 0.07650726468268232 1101 4 0.1309491419367319 1101 5 0.10564903172488727 1101 6 0.11432044208506653 1101 7 0.13370767795378669 1101 8 0.05176453267677772 1101 9 0.10323517237049629 1101 10-11 0.11360278804000501 1101 12-13 0.0788139795670233 1101 14-15 0.17305296345478638 1101 16-17 0.12594813012584893 1101 18-19 0.07359298486684906 1101 20-21 0.13645086631933623 1101 22-23 0.12963071516043811 1101 24-25 0.10986528245098981 1101 26-27 0.08616908244751897 1101 28-29 0.16967260543511742 1101 30-31 0.08323632107190093 1101 32-33 0.1315142928424038 1101 34-35 0.10427560077921783 1101 36-37 -0.03129572828345317 1101 38-39 0.007566198068481356 1101 40-41 -0.021784733063597628 1101 42-43 0.008096490084739116 1101 44-45 -0.0903149618036494 1101 46-47 0.12445249402193781 1101 48-49 0.08063767322357762 1101 50-51 0.0901810453665064 1101 52-53 0.06393080658334327 1101 54-55 0.03848957768232708 1101 56-57 -0.15207049036817466 1101 58-59 -0.20239545084150024 1101 60-61 -0.09246799312473541 1101 62-63 -0.08131199588128624 1101 64-65 -0.028316725255166375 1101 66-67 -0.08880492006926488 1101 68-69 -0.11838715303779423 1101 70-71 -0.22249579530488717 1101 72-73 -0.25932479291973465 1101 74-75 -0.26098298562538247 1101 76-77 -0.3195043892256777 1101 78-79 -0.1472291698312702 1101 80-81 0.012106981960194219 1101 82-83 0.10403218238848666 1101 84-85 0.15353258725678387 1101 86-87 0.07576274114263981 1101 88-89 0.079802148874073 1101 90-91 -0.13834767328231123 1101 92-93 -0.1617078340419269 1101 94-95 -0.08554136653392419 1101 96-97 -0.06869333226871888 1101 98-99 -0.09468967758748903 1101 100-101 -0.2029758883614221 2304 1 0.030468055154678098 2304 2 0.07027458492315475 2304 3 0.04692447282497625 2304 4 0.06664943126710199 2304 5 0.10629936366515125 2304 6 0.06192026667502404 2304 7 0.05793958157767065 2304 8 0.08291447058579138 2304 9 0.10622769339284588 2304 10-11 0.06710312434948662 2304 12-13 0.0108722496936835 2304 14-15 0.08394607155563705 2304 16-17 0.07559015093294619 2304 18-19 0.08132882439391409 2304 20-21 0.046214647993153335 2304 22-23 0.02111414059472594 2304 24-25 0.023492141673209233 2304 26-27 0.06481247655171529 2304 28-29 0.07359338871953014 2304 30-31 0.06177533497562848 2304 32-33 0.11430316825397568 2304 34-35 0.13275672455793597 2304 36-37 0.03139545303265123 2304 38-39 0.059839722295187414 2304 40-41 0.07440391450732164 2304 42-43 0.012418086925436 2304 44-45 0.04204977885323302 2304 46-47 0.02019788650191856 2304 48-49 0.15964405709098628 2304 50-51 0.060209000340314844 2304 52-53 0.043677279743121744 2304 54-55 -0.016990551197558545 2304 56-57 -0.048966325822654255 2304 58-59 0.010216964309499588 2304 60-61 0.03103719341679323 2304 62-63 -0.0355077833144577 2304 64-65 -0.016789142666937096 2304 66-67 -0.0896304856824841 2304 68-69 -0.17390342215912824 2304 70-71 -0.09820065427537372 2304 72-73 -0.20214926342270445 2304 74-75 -0.27913518350202793 2304 76-77 -0.6229113846231691 2304 78-79 -0.2074575877016187 2304 80-81 -0.023110031852141333 2304 82-83 0.02481314190067252 2304 84-85 0.056312451658733664 2304 86-87 -0.05711411988338 2304 88-89 -0.08342754448704426 2304 90-91 -0.23223553110717887 2304 92-93 -0.26620647637780337 2304 94-95 -0.22305848063783884 2304 96-97 -0.16079510216248494 2304 98-99 -0.13472920096601015 2304 100-101 -0.16266467181900168 2202 1 0.017112537055801624 2202 2 0.0853422925672831 2202 3 0.07047572737057806 2202 4 0.07139584305482316 2202 5 0.03757836456975383 2202 6 0.03194659481431472 2202 7 0.026519966282336327 2202 8 0.050002228398724924 2202 9 0.0527433763878804 2202 10-11 0.046958243657684307 2202 12-13 0.07736291336381385 2202 14-15 0.04414739757985586 2202 16-17 -0.018194238888384007 2202 18-19 -0.03348659300579726 2202 20-21 -0.03846648215723292 2202 22-23 -0.018143985804918827 2202 24-25 -0.0725448840352314 2202 26-27 0.04501523431765264 2202 28-29 -0.023751005180088214 2202 30-31 -1.3398660295393938E-4 2202 32-33 -0.03950295800974146 2202 34-35 -0.18154210886180522 2202 36-37 -0.21992577702148708 2202 38-39 -0.1225422765174784 2202 40-41 -0.03877050995781417 2202 42-43 -0.20087686416033534 2202 44-45 -0.12293174227833248 2202 46-47 -0.25324339147941544 2202 48-49 -0.2737670675421313 2202 50-51 -0.05105812723148517 2202 52-53 -0.017517747340647816 2202 54-55 -0.09982019345736859 2202 56-57 -0.10158054880267997 2202 58-59 -0.18961283197101153 2202 60-61 -0.2574002629711245 2202 62-63 -0.389430545910713 2202 64-65 -0.38413860551071366 2202 66-67 -0.13015068517183437 2202 68-69 -0.09218619982482323 2202 70-71 -0.19818136191733515 2202 72-73 -0.3326013105602712 2202 74-75 -0.22844533708163084 2202 76-77 -0.5451194195865874 2202 78-79 -0.18725744756446971 2202 80-81 -0.004577436950441438 2202 82-83 0.0014607837569968751 2202 84-85 0.025432096818178707 2202 86-87 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1102 42-43 -0.13005130664666353 1102 44-45 -0.18776338633845313 1102 46-47 -0.19021498203434106 1102 48-49 -0.0943131520234175 1102 50-51 -0.13597936478938522 1102 52-53 -0.11139955456706474 1102 54-55 -0.19034714375570871 1102 56-57 -0.2628354653558631 1102 58-59 -0.27089592249100036 1102 60-61 -0.1912138318708685 1102 62-63 -0.3096294258778727 1102 64-65 -0.28855414562795545 1102 66-67 -0.17056810502264597 1102 68-69 -0.16374896485592672 1102 70-71 -0.2922680714359984 1102 72-73 -0.31347802793031576 1102 74-75 -0.3690291264179919 1102 76-77 -0.426812763296077 1102 78-79 -0.16833670614794016 1102 80-81 -0.02078175715806907 1102 82-83 -0.02175830169328208 1102 84-85 -0.012304475411632154 1102 86-87 -0.06258731573684173 1102 88-89 -0.13763787927598514 1102 90-91 -0.10680241971196125 1102 92-93 -0.12353960769764427 1102 94-95 -0.2809274181575532 1102 96-97 -0.15438142280494205 1102 98-99 -0.13912042085078014 1102 100-101 -0.21431310192510367 2305 1 -0.012668084731494389 2305 2 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78-79 -0.15460004103500324 1213 80-81 -0.32100489148202627 1213 82-83 -0.3061568596191435 1213 84-85 -0.3457167946571573 1213 86-87 -0.35295830087133595 1213 88-89 -0.36770974065187545 1213 90-91 -0.2564714164103563 1213 92-93 -0.18483321993237212 1213 94-95 -0.017575444991834388 1213 96-97 -0.19874390078090443 1213 98-99 -0.22056919035632916 1213 100-101 0.1474483464313856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 92.0 9 294.0 10 209.0 11 98.0 12 94.0 13 182.0 14 486.0 15 1090.0 16 2067.0 17 3706.0 18 5620.0 19 8106.0 20 11106.0 21 14935.0 22 19454.0 23 25681.0 24 33116.0 25 43306.0 26 56755.0 27 77699.0 28 114013.0 29 159048.0 30 153178.0 31 156349.0 32 206727.0 33 291809.0 34 451039.0 35 782929.0 36 1470010.0 37 1957256.0 38 386399.0 39 399.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.42812563098595 25.831905109365188 21.89310496695015 15.846864292698706 2 29.58590201546339 25.01907275626976 31.110090374087168 14.284934854179685 3 35.672581890513975 19.435984958065113 27.373417696922512 17.518015454498396 4 28.745636230822697 19.300851775409118 31.754994987407503 20.19851700636068 5 33.85244805899342 17.966038991118033 30.71904446579184 17.46246848409671 6 28.82434411169454 18.96778616129303 34.82898250653634 17.378887220476088 7 31.77746585712376 21.899783313592174 27.503971547862204 18.81877928142186 8 28.435505482445915 27.83663337095034 25.22705440165639 18.50080674494735 9 31.05657162931391 23.406453337810067 25.934720499404655 19.602254533471367 10-11 25.001150272179267 28.437005499544554 29.105057156091934 17.456787072184245 12-13 23.720109714263685 27.078696458006924 28.56480228947812 20.63639153825127 14-15 23.36627153262887 27.477460882973798 28.601183536807156 20.55508404759018 16-17 24.32807006090225 26.35153872220305 29.26827145179893 20.052119765095767 18-19 21.96073996698408 26.36938348520035 31.002431115795105 20.667445432020468 20-21 23.393427282590785 26.730483456288102 30.501440949067813 19.374648312053296 22-23 21.947146162958667 29.858480572264316 28.688784255425443 19.50558900935157 24-25 21.84416851370931 26.216269612732713 30.713758209877206 21.225803663680775 26-27 21.708744866521794 26.356994742945254 29.161832414670013 22.77242797586294 28-29 22.813697876348847 28.438645414610942 28.635847144371596 20.11180956466861 30-31 20.855025290443724 27.521178997895312 30.160626183303645 21.46316952835732 32-33 20.680805687940484 28.974042709295134 28.953306721631023 21.39184488113336 34-35 22.520479508056567 29.142720797853656 29.077543889101324 19.259255804988452 36-37 22.21005368973118 29.438573911205207 29.434167125221116 18.917205273842498 38-39 21.029794875912135 27.62144085757586 31.944807901693146 19.403956364818864 40-41 20.998988071366618 27.28070216344435 28.69350842341208 23.026801341776952 42-43 21.282188190746215 28.924075818480834 30.21127701973805 19.582458971034903 44-45 20.887051940359573 27.57583701621582 30.420564027206453 21.116547016218153 46-47 22.478816347096746 27.465336670913644 28.989919252515485 21.065927729474126 48-49 21.088769530445443 29.277725798320773 30.004988266149258 19.62851640508452 50-51 22.452901032458705 27.832946578747197 29.43408491730172 20.280067471492377 52-53 20.77754046605328 27.518874831537172 28.34072471269996 23.362859989709584 54-55 22.512232628036006 27.58577995609428 29.793649331718246 20.108338084151463 56-57 21.192047318079684 27.297022551542838 29.465164209806833 22.04576592057065 58-59 21.173363208992793 27.409111728960777 29.60964867315743 21.807876388888996 60-61 22.342019087513542 27.643714980751678 29.50559200430084 20.508673927433936 62-63 20.81419143445541 27.7121844953009 29.521104140246123 21.952519929997564 64-65 22.684534766487108 27.657263641702436 29.412927794007874 20.24527379780258 66-67 21.690141523817207 30.474976151273793 27.69317273908518 20.14170958582382 68-69 21.775009492393067 30.35739199949633 27.705421409786513 20.16217709832409 70-71 21.445561710077108 30.935191908677407 27.741191098127388 19.878055283118094 72-73 21.374284201069916 31.192705189475205 27.451018188369787 19.981992421085096 74-75 21.948733725555154 30.46544673249574 27.2524260728769 20.33339346907221 76-77 22.02950250165321 29.561168397807165 27.78339815070731 20.625930949832316 78-79 21.800080740348893 29.262663713337872 28.45297512018949 20.484280426123746 80-81 21.324148906536408 29.572933014318366 28.27795805964618 20.824960019499045 82-83 20.472808498335215 29.66084846563018 28.56443700394512 21.301906032089484 84-85 20.64864722025544 29.593960114934397 28.502650688184094 21.254741976626068 86-87 20.488148106753894 29.699725819485117 29.031416157864932 20.780709915896058 88-89 20.585720893441565 29.978076136052472 28.87778683618365 20.558416134322314 90-91 20.97417052985962 29.4525042763084 28.38486317017877 21.18846202365321 92-93 20.577202957267982 29.964762819434593 28.072771687880692 21.385262535416732 94-95 20.338722334460694 30.17151965914327 28.022510497872442 21.467247508523595 96-97 20.712977420596502 30.581058510198382 27.647418115383733 21.058545953821387 98-99 20.92248064967923 30.0604428151492 27.646305649296426 21.37077088587514 100-101 21.018142536441847 29.95432148107592 27.26854768768043 21.7589882948018 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32.0 1 21.5 2 25.5 3 66.0 4 75.0 5 49.0 6 39.5 7 44.0 8 48.0 9 44.5 10 47.0 11 44.5 12 49.0 13 54.0 14 56.0 15 69.5 16 78.0 17 99.0 18 141.0 19 186.5 20 259.5 21 425.5 22 645.0 23 984.0 24 1405.0 25 2098.0 26 3469.0 27 5493.0 28 7723.5 29 10108.0 30 12958.0 31 16976.5 32 28200.5 33 50512.5 34 86617.0 35 139143.0 36 213155.5 37 306347.0 38 393635.5 39 429289.0 40 437752.0 41 427260.0 42 404888.0 43 413410.0 44 411147.0 45 396609.0 46 395059.5 47 357483.0 48 286045.0 49 235248.5 50 195901.0 51 166696.5 52 145982.0 53 118042.0 54 85948.5 55 59044.5 56 42137.5 57 31364.0 58 25065.0 59 20135.0 60 15658.0 61 12187.0 62 9382.5 63 7260.5 64 5727.5 65 4497.5 66 3348.0 67 2429.0 68 1858.0 69 1402.0 70 989.5 71 711.5 72 559.0 73 412.5 74 266.0 75 182.0 76 140.0 77 115.0 78 72.5 79 42.5 80 27.0 81 19.5 82 16.0 83 10.5 84 7.0 85 6.0 86 5.0 87 3.0 88 3.5 89 2.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15203800250572805 2 0.0 3 0.004834251996654884 4 0.007165884792469137 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.440476523566589E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3989796774885517E-4 24-25 4.663265591628506E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.782483530900353E-4 40-41 0.0 42-43 0.0 44-45 1.0103742115195095E-4 46-47 8.782483530900353E-4 48-49 0.0025337076381181547 50-51 3.8083335664966126E-4 52-53 0.0013989796774885517 54-55 0.0024482144356049655 56-57 0.004437874421366461 58-59 0.0058368540988550125 60-61 0.001577738191834311 62-63 8.627041344512736E-4 64-65 0.0012746259283784582 66-67 0.008184031113308027 68-69 0.007422364400008705 70-71 0.009186633215508156 72-73 0.006419762297808576 74-75 0.009178861106188776 76-77 0.004880884652571169 78-79 0.004554456061157173 80-81 0.001927483111206449 82-83 0.0 84-85 3.8860546596904214E-4 86-87 0.0013912075681691708 88-89 0.0049042009805293114 90-91 0.00673841877990319 92-93 0.008798027749539113 94-95 0.00820734744126617 96-97 0.010445714925247852 98-99 0.007546718149118798 100-101 0.007414592290689324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6433260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.2598326309204 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44264801497945 27.563655245058804 2 6.149102662894016 3.9673804547061575 3 2.121219825446141 2.0529058962684807 4 1.3334861842145387 1.720721644736228 5 0.7828953516553645 1.2628036505963813 6 0.6475033870227679 1.253301053598514 7 0.3332499745923418 0.7525411883225195 8 0.3728696611044845 0.9622970288302943 9 0.24155440372145434 0.7013254171784303 >10 1.9914477566778905 12.967548899951245 >50 0.27181186948991853 6.156133300814483 >100 0.246836983179837 17.493230312510683 >500 0.04637378778875484 10.386921676618817 >1k 0.018711385181608378 9.611119680813886 >5k 2.4063520778735398E-4 0.4493412109585264 >10k+ 4.811684378982375E-5 2.6987733390363475 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 173863 2.7025644851910227 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6499 0.1010218769333122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014922449893211218 0.0 0.0 2 0.0017875851434575938 0.0 0.0 3 0.002082925297594066 0.0 0.0 4 0.002269455921259206 0.0 0.0 5 0.002704694043144533 1.5544218638761686E-5 0.0 6 0.0031865648209461455 1.5544218638761686E-5 0.0 7 0.0036839798173865196 1.5544218638761686E-5 0.0 8 0.00424357168838194 1.5544218638761686E-5 0.0 9 0.004772075122099838 1.5544218638761686E-5 0.0 10-11 0.007608895023673845 1.5544218638761686E-5 0.0 12-13 0.010251412192263332 1.5544218638761686E-5 0.0 14-15 0.012769575611742724 1.5544218638761686E-5 0.0 16-17 0.015101208407556977 1.5544218638761686E-5 0.0 18-19 0.018140103151434887 1.5544218638761686E-5 0.0 20-21 0.021466565940129887 1.5544218638761686E-5 0.0 22-23 0.0252748995066265 1.5544218638761686E-5 0.0 24-25 0.02981381134914491 1.5544218638761686E-5 0.0 26-27 0.034352723191663326 1.5544218638761686E-5 0.0 28-29 0.03903930511124998 1.5544218638761686E-5 0.0 30-31 0.06764066740657147 1.5544218638761686E-5 0.0 32-33 0.07445680727966847 1.5544218638761686E-5 0.0 34-35 0.07981179060072187 1.5544218638761686E-5 0.0 36-37 0.08599838961894903 1.5544218638761686E-5 0.0 38-39 0.09281452949204602 1.5544218638761686E-5 0.0 40-41 0.10098301638671528 1.5544218638761686E-5 0.0 42-43 0.11028623124201416 1.5544218638761686E-5 0.0 44-45 0.12028116382673792 1.5544218638761686E-5 0.0 46-47 0.13134087538821687 1.5544218638761686E-5 0.0 48-49 0.14298349514864936 1.5544218638761686E-5 0.0 50-51 0.15618830888227742 1.5544218638761686E-5 0.0 52-53 0.1714371873669026 1.5544218638761686E-5 1.5544218638761686E-5 54-55 0.18814722240357146 1.5544218638761686E-5 1.5544218638761686E-5 56-57 0.20681582898872422 1.5544218638761686E-5 2.3316327958142528E-5 58-59 0.22708549009366946 1.5544218638761686E-5 3.108843727752337E-5 60-61 0.24915050845139167 1.5544218638761686E-5 3.108843727752337E-5 62-63 0.27451090116053134 1.5544218638761686E-5 3.108843727752337E-5 64-65 0.30133245042171464 1.5544218638761686E-5 3.108843727752337E-5 66-67 0.3304467719321153 1.5544218638761686E-5 3.108843727752337E-5 68-69 0.3639834236452436 1.5544218638761686E-5 3.108843727752337E-5 70-71 0.40054342588361114 1.5544218638761686E-5 3.108843727752337E-5 72-73 0.4417200610576908 1.5544218638761686E-5 3.108843727752337E-5 74-75 0.4865573597211989 1.5544218638761686E-5 3.108843727752337E-5 76-77 0.5357081790569633 1.5544218638761686E-5 4.6632655916285055E-5 78-79 0.5902139817137813 1.5544218638761686E-5 4.6632655916285055E-5 80-81 0.6540074550072592 1.5544218638761686E-5 4.6632655916285055E-5 82-83 0.725472000198966 1.5544218638761686E-5 4.6632655916285055E-5 84-85 0.8029987906597899 1.5544218638761686E-5 4.6632655916285055E-5 86-87 0.886673319592244 1.5544218638761686E-5 4.6632655916285055E-5 88-89 0.9813997879768577 1.5544218638761686E-5 6.217687455504674E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 28090 0.0 67.57094 4 TCGGAAG 28220 0.0 67.28015 3 GAAGAGC 28310 0.0 67.009155 6 GATCGGA 28975 0.0 65.63833 1 ATCGGAA 29400 0.0 64.547485 2 GGAAGAG 31340 0.0 60.71247 5 TTGTGTT 60475 0.0 46.260834 1 GCCGTCT 20995 0.0 44.841217 50-51 ATGCCGT 20930 0.0 44.79857 48-49 TGCCGTC 21000 0.0 44.649586 50-51 TATGCCG 21000 0.0 44.547455 48-49 CGTCTTC 21170 0.0 44.493664 52-53 CGTATGC 21120 0.0 44.440186 46-47 TCGTATG 21315 0.0 44.434742 44-45 TCTCGTA 21200 0.0 44.014828 42-43 CATCTCG 21120 0.0 43.921722 40-41 CTCGTAT 21230 0.0 43.86314 44-45 ATCTCGT 21180 0.0 43.75364 42-43 GTATGCC 21750 0.0 43.294907 46-47 CTGCTTG 22000 0.0 43.073463 58-59 >>END_MODULE