FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6433260
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1738632.7025644851910227TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC64990.1010218769333122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA280900.067.570944
TCGGAAG282200.067.280153
GAAGAGC283100.067.0091556
GATCGGA289750.065.638331
ATCGGAA294000.064.5474852
GGAAGAG313400.060.712475
TTGTGTT604750.046.2608341
GCCGTCT209950.044.84121750-51
ATGCCGT209300.044.7985748-49
TGCCGTC210000.044.64958650-51
TATGCCG210000.044.54745548-49
CGTCTTC211700.044.49366452-53
CGTATGC211200.044.44018646-47
TCGTATG213150.044.43474244-45
TCTCGTA212000.044.01482842-43
CATCTCG211200.043.92172240-41
CTCGTAT212300.043.8631444-45
ATCTCGT211800.043.7536442-43
GTATGCC217500.043.29490746-47
CTGCTTG220000.043.07346358-59
Produced by FastQC (version 0.11.2)