##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3117415 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68523375938077 33.0 33.0 33.0 33.0 33.0 2 32.71828325712168 33.0 33.0 33.0 33.0 33.0 3 32.75305918525445 33.0 33.0 33.0 33.0 33.0 4 36.68984976334559 37.0 37.0 37.0 37.0 37.0 5 36.68685144582932 37.0 37.0 37.0 37.0 37.0 6 36.565401141651016 37.0 37.0 37.0 37.0 37.0 7 36.48930732674347 37.0 37.0 37.0 37.0 37.0 8 36.42860254409503 37.0 37.0 37.0 37.0 37.0 9 36.451855463581204 37.0 37.0 37.0 37.0 37.0 10-11 36.38619112309397 37.0 37.0 37.0 37.0 37.0 12-13 36.37456690880104 37.0 37.0 37.0 37.0 37.0 14-15 38.21975611203513 40.0 37.0 40.0 37.0 40.0 16-17 38.210313833737246 40.0 37.0 40.0 37.0 40.0 18-19 38.195564113215596 40.0 37.0 40.0 37.0 40.0 20-21 38.15496444971234 40.0 37.0 40.0 37.0 40.0 22-23 38.00627057995166 40.0 37.0 40.0 33.0 40.0 24-25 38.012099768558244 40.0 37.0 40.0 35.0 40.0 26-27 37.93505741134882 40.0 37.0 40.0 33.0 40.0 28-29 37.75846510650651 40.0 37.0 40.0 33.0 40.0 30-31 37.711915962424 40.0 37.0 40.0 33.0 40.0 32-33 37.571369066999424 40.0 37.0 40.0 33.0 40.0 34-35 37.522113513920985 40.0 37.0 40.0 33.0 40.0 36-37 37.43244049958058 40.0 37.0 40.0 33.0 40.0 38-39 37.231665177719364 40.0 37.0 40.0 33.0 40.0 40-41 37.141535053882784 40.0 37.0 40.0 33.0 40.0 42-43 36.98132715727614 38.5 37.0 40.0 33.0 40.0 44-45 36.79916854188487 37.0 37.0 40.0 33.0 40.0 46-47 36.816030429057406 37.0 37.0 40.0 33.0 40.0 48-49 36.538636338119886 37.0 37.0 40.0 33.0 40.0 50-51 36.45591844525031 37.0 37.0 40.0 33.0 40.0 52-53 36.3670377540366 37.0 37.0 40.0 33.0 40.0 54-55 36.06463784898706 37.0 37.0 40.0 30.0 40.0 56-57 35.91283980477415 37.0 37.0 40.0 27.0 40.0 58-59 35.75920001026492 37.0 37.0 40.0 27.0 40.0 60-61 35.48191145548475 37.0 33.0 40.0 27.0 40.0 62-63 35.26327133217747 37.0 33.0 40.0 27.0 40.0 64-65 35.03107751133551 37.0 33.0 40.0 27.0 40.0 66-67 34.58540537592845 37.0 33.0 40.0 27.0 40.0 68-69 34.430369873757584 37.0 33.0 37.0 27.0 40.0 70-71 34.08355977628901 37.0 33.0 37.0 27.0 40.0 72-73 33.55480582469771 37.0 33.0 37.0 24.5 40.0 74-75 32.98729893196767 37.0 33.0 37.0 22.0 40.0 76-77 30.372469337576163 33.0 30.0 35.0 22.0 37.0 78-79 32.58842630833559 35.0 33.0 37.0 22.0 37.0 80-81 32.87013583369554 37.0 33.0 37.0 22.0 37.0 82-83 32.73512028395321 37.0 33.0 37.0 22.0 37.0 84-85 32.51204603814378 37.0 33.0 37.0 22.0 37.0 86-87 32.30617354442703 37.0 33.0 37.0 22.0 37.0 88-89 32.119244790956614 37.0 33.0 37.0 22.0 37.0 90-91 31.93204786658177 33.0 33.0 37.0 22.0 37.0 92-93 31.79541318688721 33.0 33.0 37.0 15.0 37.0 94-95 31.597345877914876 33.0 33.0 37.0 15.0 37.0 96-97 31.42737765103459 33.0 33.0 37.0 15.0 37.0 98-99 31.28282663681287 33.0 33.0 37.0 15.0 37.0 100-101 29.693953965064004 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1686327937038925 2306 2 0.16060895026036803 2306 3 0.1326064746571305 2306 4 0.16101250568318193 2306 5 0.17431328618298636 2306 6 0.15965227226832468 2306 7 0.2354452076909439 2306 8 0.14531979929508054 2306 9 0.15634520054002365 2306 10-11 0.21060541380454367 2306 12-13 0.25270576934244815 2306 14-15 0.39485402936141867 2306 16-17 0.3543956641767778 2306 18-19 0.35481720239669556 2306 20-21 0.3434784887699607 2306 22-23 0.32451659720353376 2306 24-25 0.39068883181631264 2306 26-27 0.5102803527160802 2306 28-29 0.4577749816456631 2306 30-31 0.3498259081305406 2306 32-33 0.39716315898309773 2306 34-35 0.42095676229075707 2306 36-37 0.3705803517377717 2306 38-39 0.333186824359494 2306 40-41 0.3901344925729404 2306 42-43 0.3872336790261883 2306 44-45 0.33856879980428545 2306 46-47 0.26762298164268117 2306 48-49 0.24279210873445578 2306 50-51 0.023663370767380343 2306 52-53 0.28327731737631723 2306 54-55 0.07537699213718696 2306 56-57 0.2622160199877186 2306 58-59 0.19552297195866686 2306 60-61 0.22683537384655494 2306 62-63 0.2968667574333921 2306 64-65 0.12665290803479223 2306 66-67 0.3061721669425239 2306 68-69 0.28640346886628976 2306 70-71 0.3669071169781546 2306 72-73 0.3996152628767504 2306 74-75 0.32502989983576214 2306 76-77 -0.006377175511236288 2306 78-79 0.27141229740811923 2306 80-81 0.36516956501436226 2306 82-83 0.3284144512021925 2306 84-85 0.3811202748951885 2306 86-87 0.4825527843844313 2306 88-89 0.472465621112363 2306 90-91 0.40557802324130776 2306 92-93 0.3498185331817609 2306 94-95 0.16610705882196442 2306 96-97 0.5084736686075715 2306 98-99 0.5100633103787722 2306 100-101 0.32826817816745546 2307 1 0.1252730084453617 2307 2 0.12419070202057725 2307 3 0.12847420865140435 2307 4 0.1582325415212722 2307 5 0.1278828606475102 2307 6 0.15459713950230736 2307 7 0.20789527240525274 2307 8 0.22289981896057043 2307 9 0.2049425387602284 2307 10-11 0.24610946771957742 2307 12-13 0.20280906104011365 2307 14-15 0.3763337442356658 2307 16-17 0.3470006185896679 2307 18-19 0.2854912480101177 2307 20-21 0.3033503819446892 2307 22-23 0.3456764427504524 2307 24-25 0.35476999624329864 2307 26-27 0.47928970339588517 2307 28-29 0.3988792757572597 2307 30-31 0.29409339833965475 2307 32-33 0.39503273033749764 2307 34-35 0.41390203503586775 2307 36-37 0.35304035087650476 2307 38-39 0.38910369816778 2307 40-41 0.37506165009546777 2307 42-43 0.33203721546597365 2307 44-45 0.16159818321205677 2307 46-47 0.052057933591406425 2307 48-49 -0.07928465248767225 2307 50-51 -0.10080865733849009 2307 52-53 0.0198951512531238 2307 54-55 -0.10402340165119028 2307 56-57 -0.05469810017276444 2307 58-59 -0.1831632617162242 2307 60-61 -0.04751772227274387 2307 62-63 -0.0905699636921895 2307 64-65 -0.044835533103892544 2307 66-67 -0.12533891822438648 2307 68-69 -0.10561738206112636 2307 70-71 0.18607980849543537 2307 72-73 0.10724906958550662 2307 74-75 0.03436484963133779 2307 76-77 -0.22548954874415372 2307 78-79 -0.16694029271942412 2307 80-81 0.0046728677170477795 2307 82-83 0.22380902907588052 2307 84-85 0.20201280009310807 2307 86-87 0.057948247444635115 2307 88-89 0.19179046901578545 2307 90-91 0.20149513030820643 2307 92-93 0.2564047395833384 2307 94-95 0.3168740609823004 2307 96-97 0.3658983799051825 2307 98-99 0.015840797214615066 2307 100-101 -0.09720001373140974 2201 1 0.12750726843353277 2201 2 0.1373114854757631 2201 3 0.12069525969245376 2201 4 0.14832544106851486 2201 5 0.15699065725521422 2201 6 0.17703960620231385 2201 7 0.13982433263508653 2201 8 0.18273333265234015 2201 9 0.2004599750125564 2201 10-11 0.132282750692994 2201 12-13 0.12441191827139164 2201 14-15 0.3111127797978668 2201 16-17 0.23700720095730077 2201 18-19 0.15861422645474477 2201 20-21 0.1214202164180378 2201 22-23 0.20789040168698847 2201 24-25 0.28022578814165655 2201 26-27 0.3026680834260489 2201 28-29 0.17426358746032378 2201 30-31 0.3056221642744532 2201 32-33 0.21612587669103078 2201 34-35 0.27913605497958116 2201 36-37 0.20525280400867274 2201 38-39 0.25185080027458184 2201 40-41 0.07918344496225416 2201 42-43 0.22357365866243128 2201 44-45 0.0354731535966053 2201 46-47 0.08278336459864732 2201 48-49 -0.09237539787643811 2201 50-51 -0.02992870323561192 2201 52-53 -0.06036578201790377 2201 54-55 -0.05870948305149426 2201 56-57 -0.09803886890279756 2201 58-59 0.11390203219780659 2201 60-61 -0.10948690347666457 2201 62-63 -0.13706225873309563 2201 64-65 -0.16912863077233453 2201 66-67 -0.2761124569504716 2201 68-69 -0.173036716849289 2201 70-71 -0.045161679093645546 2201 72-73 -0.15804895354562376 2201 74-75 -0.22220444948825246 2201 76-77 -0.2667696541036477 2201 78-79 -0.13221615790025965 2201 80-81 -0.11025199420537035 2201 82-83 -0.049240895750934044 2201 84-85 0.0055997353286940665 2201 86-87 -0.13572888261186478 2201 88-89 -0.06854438130491758 2201 90-91 -0.057739884662712626 2201 92-93 -0.17491861799110353 2201 94-95 -0.22716447512618743 2201 96-97 0.004131871126915598 2201 98-99 -0.028324002800779624 2201 100-101 -0.13007492195926673 1101 1 0.10438235504587112 1101 2 0.07546049781333863 1101 3 0.06235053828745407 1101 4 0.08648638850303314 1101 5 0.07077881061059799 1101 6 0.13672616403415816 1101 7 0.1711513098662394 1101 8 0.0090120291010507 1101 9 0.1110475332044416 1101 10-11 0.10993593931990375 1101 12-13 0.11018892955072346 1101 14-15 0.12100193590032404 1101 16-17 0.0857825605518272 1101 18-19 0.0790230433086947 1101 20-21 0.05269892281266664 1101 22-23 0.2123103659214749 1101 24-25 0.14581040565825987 1101 26-27 0.13847993855784324 1101 28-29 0.081822946333439 1101 30-31 0.17925998845323932 1101 32-33 0.1531340926791458 1101 34-35 0.16967497102081808 1101 36-37 0.10110416431134439 1101 38-39 -0.025066655931901494 1101 40-41 -0.041928282795524296 1101 42-43 0.12461247623911476 1101 44-45 -0.1616210481343785 1101 46-47 0.1060763639349247 1101 48-49 0.07459992365838986 1101 50-51 0.02432758440552618 1101 52-53 0.05480790348035214 1101 54-55 -0.06497375129412575 1101 56-57 -0.0975064309669662 1101 58-59 0.013412162774393721 1101 60-61 -0.1445923334816257 1101 62-63 -0.0902531400538038 1101 64-65 -0.05729246252195708 1101 66-67 -0.038541368529308784 1101 68-69 0.027333337462032148 1101 70-71 -0.06393193974952993 1101 72-73 -0.03217379598576997 1101 74-75 -0.07717147215233666 1101 76-77 -0.16313820194050876 1101 78-79 0.0744277790373431 1101 80-81 0.05823576933648411 1101 82-83 0.1686445763849278 1101 84-85 0.07860241343183105 1101 86-87 0.016062343367927667 1101 88-89 0.05116928245820418 1101 90-91 -0.11262641172853094 1101 92-93 -0.15817566616314593 1101 94-95 -0.08088892158633598 1101 96-97 -0.0473783496259621 1101 98-99 -0.05994902592503237 1101 100-101 -0.143601033630663 2304 1 0.057297293887316414 2304 2 0.068994129736339 2304 3 0.10624090469541159 2304 4 0.0958549662844348 2304 5 0.11568113502816857 2304 6 0.04067417548803576 2304 7 0.056796029756462474 2304 8 0.05909740991356216 2304 9 0.0380961611913051 2304 10-11 -0.022522974413440977 2304 12-13 -0.024507897288401637 2304 14-15 0.059408174150192394 2304 16-17 0.012578199036283877 2304 18-19 -0.04045374387723655 2304 20-21 0.05085294637716231 2304 22-23 0.09945323312689425 2304 24-25 0.019359594297363003 2304 26-27 -0.042962841203937785 2304 28-29 0.02420582401732929 2304 30-31 -0.09117069577729353 2304 32-33 0.018600782422616646 2304 34-35 -0.05554086248274359 2304 36-37 -0.1244938787221983 2304 38-39 -0.05284954884380255 2304 40-41 -0.0026419563708088845 2304 42-43 -0.16384804951616871 2304 44-45 -0.10997521599597349 2304 46-47 -0.06932753879500808 2304 48-49 -0.11322159606304893 2304 50-51 -0.24670878201786195 2304 52-53 -0.2222100672436369 2304 54-55 -0.1912218020925991 2304 56-57 -0.2050958980169426 2304 58-59 -0.21967963560278037 2304 60-61 -0.20682364219456417 2304 62-63 -0.14867325457242941 2304 64-65 -0.2130438522451925 2304 66-67 -0.20247554731884065 2304 68-69 -0.19071065872895332 2304 70-71 -0.1712792345479599 2304 72-73 -0.23841768088485793 2304 74-75 -0.2593550239605946 2304 76-77 -0.5416257590832672 2304 78-79 -0.20315059236433086 2304 80-81 -0.0034301074193052727 2304 82-83 -0.01823383000090928 2304 84-85 0.019352389365543843 2304 86-87 -0.09582366863536862 2304 88-89 -0.16218883160358644 2304 90-91 -0.20622506455314138 2304 92-93 -0.28156326687678046 2304 94-95 -0.24761219014697744 2304 96-97 -0.32594159398279743 2304 98-99 -0.2627002804745686 2304 100-101 -0.20429067446103844 2202 1 0.0765906295883454 2202 2 0.06798819726952843 2202 3 0.05866167502096431 2202 4 0.070375109701871 2202 5 0.06960450339701652 2202 6 0.12287544251393001 2202 7 0.0758857166384388 2202 8 0.13793818173969186 2202 9 0.12823886310418686 2202 10-11 0.16356258242413446 2202 12-13 0.08804653097261195 2202 14-15 0.12086333331636467 2202 16-17 0.0699258368403406 2202 18-19 0.03165314655014129 2202 20-21 0.03613837772303441 2202 22-23 0.10347435582574604 2202 24-25 0.05012606414213394 2202 26-27 0.15814855122386717 2202 28-29 0.1814094592211717 2202 30-31 0.050429760882735764 2202 32-33 0.1768814678529722 2202 34-35 -0.0025671117436800728 2202 36-37 -0.0266897228378582 2202 38-39 0.17040097599367954 2202 40-41 0.23317678575155298 2202 42-43 0.059991405689920896 2202 44-45 0.10939953577986472 2202 46-47 -0.09861016772455145 2202 48-49 -0.04987783309058358 2202 50-51 0.08162004332821482 2202 52-53 0.2116391909155766 2202 54-55 0.16953903916531488 2202 56-57 0.10797642975858679 2202 58-59 0.17432578904556095 2202 60-61 2.833684522443036E-4 2202 62-63 -0.07250374401616 2202 64-65 -0.063698222540161 2202 66-67 0.16077383615825624 2202 68-69 0.06693862845672527 2202 70-71 0.08002130794584872 2202 72-73 -0.123619711811088 2202 74-75 0.01569798122100252 2202 76-77 -0.2647307170194999 2202 78-79 0.10010422561041565 2202 80-81 0.2781413256629435 2202 82-83 0.33804347827734205 2202 84-85 0.33995936133936056 2202 86-87 0.22837996454067166 2202 88-89 0.3749188511132928 2202 90-91 0.5140161361811515 2202 92-93 0.4182204250532493 2202 94-95 0.38542516681000194 2202 96-97 0.3668575662772362 2202 98-99 0.3910514637935023 2202 100-101 0.2671175238088992 1102 1 0.09828411394762782 1102 2 0.0650046848575272 1102 3 0.08451913545604839 1102 4 -0.007096837580192528 1102 5 0.03292703025881849 1102 6 0.01879072859872366 1102 7 0.03103762475255678 1102 8 -0.0026642900752662513 1102 9 0.05924465890156938 1102 10-11 0.00972948286344888 1102 12-13 -0.001362443250648937 1102 14-15 -0.005604670706290449 1102 16-17 -0.06414021937095526 1102 18-19 -0.0042298524441974905 1102 20-21 -0.05295262158887937 1102 22-23 -0.08932734321623315 1102 24-25 -0.061543734195872446 1102 26-27 -0.01514618756828412 1102 28-29 -0.10289444963395766 1102 30-31 -0.0701613972181434 1102 32-33 0.06791637496143466 1102 34-35 0.021814074409924444 1102 36-37 0.09282391978110383 1102 38-39 0.07983628272103971 1102 40-41 0.06737298275574233 1102 42-43 0.012909880786516226 1102 44-45 -0.1385418975552284 1102 46-47 -0.053357891749321595 1102 48-49 -0.08102051821204981 1102 50-51 0.00956299839093333 1102 52-53 -0.001157347484891602 1102 54-55 -0.10591701598738723 1102 56-57 -0.035873011833537305 1102 58-59 -0.0409352203229858 1102 60-61 -0.009213204352498394 1102 62-63 0.06564722459656025 1102 64-65 0.01005845982896858 1102 66-67 -0.11530295154089742 1102 68-69 0.006262981391671474 1102 70-71 -0.1632841491017345 1102 72-73 -0.2391643579763283 1102 74-75 -0.25703848201934676 1102 76-77 -0.4873724361747449 1102 78-79 -0.162107506247942 1102 80-81 -0.0907410646403477 1102 82-83 -0.015550511560164182 1102 84-85 -0.14525751792809416 1102 86-87 -0.08065473084915453 1102 88-89 -0.11706806202914066 1102 90-91 0.01926708891497242 1102 92-93 -0.03273647697395887 1102 94-95 -0.12901311721053332 1102 96-97 -0.043131781629394794 1102 98-99 -0.01731228203829005 1102 100-101 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-0.0018648894363835211 1213 66-67 0.12070231487794558 1213 68-69 0.10853801532098117 1213 70-71 -0.018799579561409985 1213 72-73 0.19218425829793517 1213 74-75 0.26423312947530775 1213 76-77 0.2711529665514014 1213 78-79 -0.23484405253932294 1213 80-81 -0.35333591918686835 1213 82-83 -0.34924386436596677 1213 84-85 -0.3365599608383505 1213 86-87 -0.38145679021069157 1213 88-89 -0.605128411943582 1213 90-91 -0.3750530709953388 1213 92-93 -0.2331925311428371 1213 94-95 -0.08512231057808961 1213 96-97 -0.2669695552766491 1213 98-99 -0.35034563602424384 1213 100-101 0.03401112446476873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 11.0 10 15.0 11 20.0 12 40.0 13 150.0 14 349.0 15 785.0 16 1600.0 17 2701.0 18 4101.0 19 5668.0 20 7752.0 21 10302.0 22 13518.0 23 17284.0 24 21969.0 25 27771.0 26 35369.0 27 48045.0 28 68856.0 29 82342.0 30 71119.0 31 75308.0 32 98690.0 33 136175.0 34 200810.0 35 320159.0 36 557076.0 37 901374.0 38 407011.0 39 1043.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.99453782417011 26.360058329175608 19.52665212185388 16.118751724800408 2 29.544991603620307 27.616214074802357 28.486326010492668 14.352468311084666 3 34.849475904435764 21.01357756373756 27.482625049121427 16.65432148270525 4 29.44793935641579 21.645403130343286 28.25309649913545 20.653561014105474 5 34.28812012516781 19.926060534128435 28.77178688111785 17.014032459585906 6 30.97614529987185 20.817343857009735 31.031255062287183 17.175255780831232 7 31.147088212509406 24.803082040729258 25.128544002001657 18.92128574475968 8 28.647356864581713 30.194504100352376 23.060484407754505 18.09765462731141 9 31.748227297295994 24.51685130147895 24.832593671359124 18.90232772986593 10-11 26.359916790032766 29.99125878331887 27.953127831873527 15.695696594774839 12-13 23.99598256695815 29.356804928188062 27.71868409500376 18.928528409850024 14-15 23.28199806570508 29.671538758875542 27.93205267826068 19.114410497158705 16-17 24.885409866828766 27.674579739944793 28.10099072468696 19.33901966853948 18-19 22.02396857652895 27.707956752629983 30.573279463914815 19.69479520692625 20-21 23.964983808700477 27.759297366568138 30.471207715366738 17.804511109364647 22-23 21.600985561416188 32.498570127583434 27.96854505228858 17.9318992587118 24-25 21.317075627427094 27.93937539255293 30.432499501189124 20.31104947883085 26-27 21.293138706267854 27.928299568713182 28.19732053640596 22.581241188613003 28-29 23.28663331638553 30.367211295255846 28.056787434460922 18.2893679538977 30-31 20.884691322778647 28.1767586285432 30.360474944785988 20.578075103892168 32-33 20.80149739447587 30.451688337933835 28.137094355419475 20.60971991217082 34-35 20.722890600064474 28.243528692843267 30.42437404067152 20.609206666420736 36-37 22.970457895403722 28.113902063087526 28.1923805460614 20.723259495447348 38-39 22.845842676733614 30.485101911162086 28.144559089612116 18.524496322492183 40-41 22.706408995914884 30.410628677927065 28.149540564859027 18.733421761299024 42-43 20.50033441168404 30.347242827791614 30.343938808275446 18.808483952248896 44-45 20.31593834886117 28.138276236827213 30.509457845161307 21.03632756915031 46-47 22.56039145561007 28.122832221372327 28.147724835884375 21.169051487133228 48-49 20.28224898423471 30.44683087002631 30.22487981794817 19.046040327790813 50-51 22.507195482143416 28.104795410930393 30.21855500123099 19.1694541056952 52-53 20.30671915305788 28.273939698390908 27.922522747070094 23.496818401481114 54-55 22.309596654222922 28.176463276247148 30.373127924647843 19.14081214488209 56-57 20.145977861767246 28.168440732141242 30.301168759595576 21.384412646495935 58-59 20.07005780354238 28.47242294010521 30.245505461005628 21.21201379534678 60-61 22.153674609343298 28.296928284947064 30.288489144245855 19.26090796146378 62-63 20.010852005969085 28.37709742361894 30.17362888769986 21.43842168271211 64-65 22.100576540545013 28.370090078645994 30.219074500535225 19.310258880273768 66-67 20.074527908908625 32.43802212964334 27.919350614000322 19.568099347447717 68-69 19.99106260809841 32.67396275648286 27.88155100641096 19.453423629007773 70-71 19.927380131663412 32.62730772673218 27.921532559281736 19.523779582322675 72-73 19.87200009752129 32.533086664348396 27.909711324146656 19.685201913983654 74-75 19.812799468239366 32.20072744957642 28.1112937541223 19.875179328061922 76-77 19.90588662510939 31.846444876726814 28.10568042128711 20.141988076876686 78-79 19.864164806353692 31.71357521091121 28.211898924295873 20.210361058439226 80-81 19.845655399173985 31.438870844861462 28.281470788724487 20.434002967240065 82-83 19.87414251872144 31.433800119650417 28.132074170426456 20.559983191201685 84-85 19.914470570328252 31.219589103955485 28.29505872910703 20.570881596609233 86-87 20.04526551023783 30.909966351600193 28.297969018579515 20.746799119582455 88-89 20.07409597093133 31.057641551999104 28.192092633757614 20.676169843311946 90-91 20.116509351720847 30.793047292646865 28.21547958536029 20.874963770272007 92-93 20.14046281993908 30.820368953498257 28.088906853757944 20.950261372804718 94-95 19.99366422645819 30.754502409197936 28.23275226002656 21.01908110431731 96-97 20.076698179926012 30.639267524040765 28.18704629079062 21.096988005242604 98-99 20.111438377268858 30.703409443967793 28.093984467341848 21.091167711421505 100-101 20.201857697765544 30.649575081590463 27.95094258697302 21.197624633670976 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 5.5 2 8.5 3 17.5 4 21.5 5 17.0 6 12.5 7 13.5 8 11.0 9 14.0 10 21.0 11 26.5 12 42.0 13 52.5 14 69.0 15 111.0 16 201.0 17 357.0 18 605.5 19 1041.0 20 1777.0 21 2937.0 22 4559.5 23 6839.0 24 9808.0 25 13693.0 26 18762.5 27 24642.5 28 31297.5 29 38776.5 30 47116.5 31 55567.5 32 64881.0 33 75993.5 34 88120.0 35 100695.5 36 114136.0 37 127984.0 38 140583.0 39 150049.5 40 156598.0 41 160018.5 42 159762.5 43 157220.5 44 151752.5 45 144208.5 46 135816.0 47 125868.5 48 114391.0 49 103035.5 50 92598.5 51 82719.0 52 72974.0 53 63004.5 54 53300.5 55 44722.5 56 37161.5 57 30314.0 58 24744.5 59 20116.0 60 16091.5 61 12664.5 62 9642.5 63 7298.5 64 5488.5 65 4030.0 66 2949.5 67 2182.0 68 1540.0 69 1098.0 70 823.5 71 599.0 72 466.0 73 350.5 74 251.5 75 183.5 76 131.0 77 98.0 78 82.0 79 65.5 80 44.5 81 29.5 82 26.5 83 25.0 84 13.5 85 9.0 86 12.0 87 7.5 88 4.0 89 5.0 90 3.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1519207420250432 2 0.0 3 0.0044909003132402965 4 0.00593440398535325 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.8116789070431755E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.2831143752115135E-4 24-25 3.2077859380287837E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.93440398535325E-4 40-41 0.0 42-43 0.0 44-45 9.623357814086351E-5 46-47 7.85907554817052E-4 48-49 0.001956749422197558 50-51 2.4058394535215875E-4 52-53 0.0011387640080002181 54-55 0.0016840876174651113 56-57 0.0029832409223667688 58-59 0.004234277438197994 60-61 0.001154802937690362 62-63 6.094793282254688E-4 64-65 8.981800626480593E-4 66-67 0.006207065790085696 68-69 0.005597586461860227 70-71 0.0071854405011844745 72-73 0.004715445328902311 74-75 0.007153362641804188 76-77 0.0035606423912119497 78-79 0.003464408813071086 80-81 0.0012831143752115135 82-83 0.0 84-85 2.726618047324466E-4 86-87 9.78374711098779E-4 88-89 0.003416292024000654 90-91 0.0049560292742544705 92-93 0.007057129063663323 94-95 0.006255182579156127 96-97 0.00838836022794527 98-99 0.005661742180620803 100-101 0.005437197164958788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3117415.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.15280354782973 #Duplication Level Percentage of deduplicated Percentage of total 1 97.78835542075606 88.15894395512774 2 1.5368525544412195 2.7710313284503916 3 0.29756742486353616 0.8047961278786788 4 0.11340497934936263 0.4089510729851511 5 0.05878235401788458 0.26496970069266645 6 0.037902932126308915 0.2050233356321911 7 0.019136287008281152 0.120763294630472 8 0.020119968392115026 0.14510972462743119 9 0.007461799734999675 0.06054319490604063 >10 0.10769104627820132 2.0186345893435176 >50 0.009154018598530997 0.5646218591652773 >100 0.003338373693447912 0.4633255497284166 >500 1.2433989569087748E-4 0.08410715607529769 >1k 7.291246759539358E-5 0.1314697401268551 >5k 0.0 0.0 >10k+ 3.558837676667065E-5 3.797709370629905 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 118368 3.7969920591259103 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017642822659158309 0.0 0.0 2 0.002085060859718709 0.0 0.0 3 0.0024699951722821634 0.0 0.0 4 0.002694540187944178 0.0 0.0 5 0.0035927202505922373 0.0 0.0 6 0.004330511016338858 0.0 0.0 7 0.0052928467977474926 0.0 3.2077859380287837E-5 8 0.006158949001015265 0.0 3.2077859380287837E-5 9 0.006832584048001309 0.0 3.2077859380287837E-5 10-11 0.010040369986030091 0.0 3.2077859380287837E-5 12-13 0.012911338400565853 0.0 3.2077859380287837E-5 14-15 0.015862501463552333 0.0 6.415571876057567E-5 16-17 0.019182559909412124 0.0 6.415571876057567E-5 18-19 0.022695085511553643 0.0 6.415571876057567E-5 20-21 0.027121830106033366 0.0 9.623357814086351E-5 22-23 0.03164480827865395 0.0 1.2831143752115135E-4 24-25 0.036873499357640865 0.0 1.2831143752115135E-4 26-27 0.043144720866487137 0.0 1.2831143752115135E-4 28-29 0.049431981305023555 0.0 1.2831143752115135E-4 30-31 0.0813334124587198 0.0 1.2831143752115135E-4 32-33 0.09057183596024271 1.6038929690143918E-5 1.2831143752115135E-4 34-35 0.09960175337579373 3.2077859380287837E-5 1.2831143752115135E-4 36-37 0.11021952483066899 3.2077859380287837E-5 1.2831143752115135E-4 38-39 0.1208854130746147 3.2077859380287837E-5 1.2831143752115135E-4 40-41 0.13344389502199738 3.2077859380287837E-5 1.6038929690143918E-4 42-43 0.1476223088680846 3.2077859380287837E-5 1.764282265915831E-4 44-45 0.1616724112766507 3.2077859380287837E-5 2.085060859718709E-4 46-47 0.17750283488082275 4.8116789070431755E-5 2.2454501566201483E-4 48-49 0.19394273781322025 6.415571876057567E-5 2.2454501566201483E-4 50-51 0.21275640233975907 6.415571876057567E-5 2.2454501566201483E-4 52-53 0.23145779435846686 6.415571876057567E-5 2.2454501566201483E-4 54-55 0.25234048081503424 6.415571876057567E-5 2.8870073442259053E-4 56-57 0.27439400913898215 6.415571876057567E-5 3.047396641127345E-4 58-59 0.29960720661188833 6.415571876057567E-5 3.2077859380287837E-4 60-61 0.32591105130372444 6.415571876057567E-5 3.2077859380287837E-4 62-63 0.35444430722249043 6.415571876057567E-5 3.2077859380287837E-4 64-65 0.38533528580570764 6.415571876057567E-5 3.3681752349302226E-4 66-67 0.4201878800223904 6.415571876057567E-5 3.528564531831662E-4 68-69 0.4573821579738341 6.415571876057567E-5 3.8493431256345404E-4 70-71 0.4983135065430814 6.415571876057567E-5 3.8493431256345404E-4 72-73 0.542853614292611 6.415571876057567E-5 3.8493431256345404E-4 74-75 0.5895750164800002 6.415571876057567E-5 4.0097324225359793E-4 76-77 0.6417977715511088 6.415571876057567E-5 4.3305110163388577E-4 78-79 0.7013503174906133 6.415571876057567E-5 4.811678907043175E-4 80-81 0.7683609657360344 6.415571876057567E-5 4.811678907043175E-4 82-83 0.8434391956155982 6.415571876057567E-5 5.132457500846054E-4 84-85 0.9247726080743179 8.019464845071959E-5 5.774014688451811E-4 86-87 1.016178468378448 9.623357814086351E-5 6.09479328225469E-4 88-89 1.1173359979341857 9.623357814086351E-5 6.415571876057567E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTCG 20 0.002073819 71.25513 2 TCGGAAG 18360 0.0 71.17751 3 CGGAAGA 18325 0.0 71.127426 4 GATCGGA 18585 0.0 70.57557 1 ATCGGAA 18660 0.0 70.058624 2 GAAGAGC 19465 0.0 66.93304 6 GAGCACA 19545 0.0 66.65907 9 AGAGCAC 19565 0.0 66.42099 8 AAGAGCA 21245 0.0 61.213303 7 GGAAGAG 22815 0.0 57.438137 5 TCGGACG 100 1.2732926E-11 47.503414 3 GCCGTCT 14285 0.0 44.40723 50-51 ATGCCGT 14205 0.0 44.305504 48-49 TCTCGTA 14160 0.0 44.093384 42-43 CGTCTTC 14360 0.0 44.059525 52-53 TCGTATG 14585 0.0 44.01348 44-45 TGCCGTC 14215 0.0 43.823944 50-51 AATCTCG 14320 0.0 43.716114 40-41 TATGCCG 14385 0.0 43.420914 48-49 CGTATGC 14450 0.0 43.356384 46-47 >>END_MODULE