FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117415
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1183683.7969920591259103TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG200.00207381971.255132
TCGGAAG183600.071.177513
CGGAAGA183250.071.1274264
GATCGGA185850.070.575571
ATCGGAA186600.070.0586242
GAAGAGC194650.066.933046
GAGCACA195450.066.659079
AGAGCAC195650.066.420998
AAGAGCA212450.061.2133037
GGAAGAG228150.057.4381375
TCGGACG1001.2732926E-1147.5034143
GCCGTCT142850.044.4072350-51
ATGCCGT142050.044.30550448-49
TCTCGTA141600.044.09338442-43
CGTCTTC143600.044.05952552-53
TCGTATG145850.044.0134844-45
TGCCGTC142150.043.82394450-51
AATCTCG143200.043.71611440-41
TATGCCG143850.043.42091448-49
CGTATGC144500.043.35638446-47
Produced by FastQC (version 0.11.2)