##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032612 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6853065937878 33.0 33.0 33.0 33.0 33.0 2 32.72025765247912 33.0 33.0 33.0 33.0 33.0 3 32.75016520412107 33.0 33.0 33.0 33.0 33.0 4 36.686181417207344 37.0 37.0 37.0 37.0 37.0 5 36.68630210524788 37.0 37.0 37.0 37.0 37.0 6 36.564985893348705 37.0 37.0 37.0 37.0 37.0 7 36.50180009839703 37.0 37.0 37.0 37.0 37.0 8 36.44449866979357 37.0 37.0 37.0 37.0 37.0 9 36.4620386650188 37.0 37.0 37.0 37.0 37.0 10-11 36.39833186705059 37.0 37.0 37.0 37.0 37.0 12-13 36.38053813016634 37.0 37.0 37.0 37.0 37.0 14-15 38.22523784776951 40.0 37.0 40.0 37.0 40.0 16-17 38.19130340445794 40.0 37.0 40.0 37.0 40.0 18-19 38.1931122411967 40.0 37.0 40.0 37.0 40.0 20-21 38.15558551506095 40.0 37.0 40.0 37.0 40.0 22-23 38.05282063778684 40.0 37.0 40.0 35.0 40.0 24-25 38.01554485044575 40.0 37.0 40.0 35.0 40.0 26-27 37.91722696474194 40.0 37.0 40.0 33.0 40.0 28-29 37.78033243289943 40.0 37.0 40.0 33.0 40.0 30-31 37.733620885230295 40.0 37.0 40.0 33.0 40.0 32-33 37.59671118494552 40.0 37.0 40.0 33.0 40.0 34-35 37.52551546323763 40.0 37.0 40.0 33.0 40.0 36-37 37.44747498196274 40.0 37.0 40.0 33.0 40.0 38-39 37.316013720185765 40.0 37.0 40.0 33.0 40.0 40-41 37.25447073347992 40.0 37.0 40.0 33.0 40.0 42-43 37.10695664331606 40.0 37.0 40.0 33.0 40.0 44-45 36.988989194793135 40.0 37.0 40.0 33.0 40.0 46-47 36.93878659716442 38.5 37.0 40.0 33.0 40.0 48-49 36.71686717588666 37.0 37.0 40.0 33.0 40.0 50-51 36.594390743029436 37.0 37.0 40.0 33.0 40.0 52-53 36.45792307093687 37.0 37.0 40.0 33.0 40.0 54-55 36.25424666920793 37.0 37.0 40.0 33.0 40.0 56-57 36.09194103960546 37.0 37.0 40.0 33.0 40.0 58-59 35.8609578145836 37.0 37.0 40.0 27.0 40.0 60-61 35.6259841351284 37.0 35.0 40.0 27.0 40.0 62-63 35.38522748706396 37.0 33.0 40.0 27.0 40.0 64-65 35.12771135905285 37.0 33.0 40.0 27.0 40.0 66-67 34.78149331335496 37.0 33.0 38.5 27.0 40.0 68-69 34.49890045281097 37.0 33.0 37.0 27.0 40.0 70-71 34.1064643284403 37.0 33.0 37.0 27.0 40.0 72-73 33.65750036602111 37.0 33.0 37.0 27.0 40.0 74-75 33.19868285161438 37.0 33.0 37.0 22.0 38.5 76-77 30.571445836130703 33.0 30.0 35.0 22.0 37.0 78-79 32.8927915935174 35.0 33.0 37.0 27.0 37.0 80-81 33.22949440943978 37.0 33.0 37.0 27.0 37.0 82-83 33.125858335982315 37.0 33.0 37.0 27.0 37.0 84-85 32.910115273566156 37.0 33.0 37.0 27.0 37.0 86-87 32.71779723222093 37.0 33.0 37.0 24.5 37.0 88-89 32.54140589036778 37.0 33.0 37.0 22.0 37.0 90-91 32.3567770951246 33.0 33.0 37.0 22.0 37.0 92-93 32.215372095078436 33.0 33.0 37.0 22.0 37.0 94-95 32.0123527177232 33.0 33.0 37.0 22.0 37.0 96-97 31.850946312947386 33.0 33.0 37.0 22.0 37.0 98-99 31.7036956920305 33.0 33.0 37.0 22.0 37.0 100-101 30.069762963412398 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1470790083791016 2306 2 0.1235927642050001 2306 3 0.12490945299003897 2306 4 0.15896137042043534 2306 5 0.08418415680669256 2306 6 0.15333171410774327 2306 7 0.1588672339385795 2306 8 0.24336302006940258 2306 9 0.18818350461867084 2306 10-11 0.2241761004813938 2306 12-13 0.18876348187882996 2306 14-15 0.3618123747216302 2306 16-17 0.399004910571378 2306 18-19 0.2849954664706331 2306 20-21 0.29970541710332554 2306 22-23 0.3928170564608493 2306 24-25 0.4729350993736432 2306 26-27 0.45000266151902935 2306 28-29 0.398644420881034 2306 30-31 0.39633545107232493 2306 32-33 0.4687328239506243 2306 34-35 0.37337962221210574 2306 36-37 0.4204703721515628 2306 38-39 0.36231370777337446 2306 40-41 0.4621234781374781 2306 42-43 0.45362544078327005 2306 44-45 0.42071969554937283 2306 46-47 0.38581980067093724 2306 48-49 0.3776372793144489 2306 50-51 0.3722523192492915 2306 52-53 0.23513153083751348 2306 54-55 0.43331534605304967 2306 56-57 0.38750874299791604 2306 58-59 0.39246091109003345 2306 60-61 0.39825968759054575 2306 62-63 0.30909931518265665 2306 64-65 0.15388912949291722 2306 66-67 0.21013965814380242 2306 68-69 0.2504939678403062 2306 70-71 0.21949597809692278 2306 72-73 0.17684471045220818 2306 74-75 0.2150814259574645 2306 76-77 0.013273989013935505 2306 78-79 -0.004743390711595907 2306 80-81 0.20474695375494179 2306 82-83 0.17502051342507485 2306 84-85 0.2826421979297251 2306 86-87 -0.026389005909869923 2306 88-89 0.15787527622276087 2306 90-91 0.346471496645691 2306 92-93 0.15037756937712743 2306 94-95 0.010303274981701804 2306 96-97 0.24396583085119872 2306 98-99 0.30499635618832954 2306 100-101 0.22456691345034585 2307 1 0.11559544933420796 2307 2 0.12659367714648795 2307 3 0.11699256124276758 2307 4 0.1506970294491623 2307 5 0.1296264747753284 2307 6 0.1357024426117519 2307 7 0.21669832811424783 2307 8 0.13882521308008222 2307 9 0.19804603921799213 2307 10-11 0.14587343999224345 2307 12-13 0.19881093445173548 2307 14-15 0.34891753116659885 2307 16-17 0.2876450682030267 2307 18-19 0.24264788547437632 2307 20-21 0.33580837637332195 2307 22-23 0.3882025703945047 2307 24-25 0.33521843957466757 2307 26-27 0.37149260533016815 2307 28-29 0.33676333187433727 2307 30-31 0.38709883469266515 2307 32-33 0.34346199920189946 2307 34-35 0.39841116383102104 2307 36-37 0.3116342233086087 2307 38-39 0.27459442708973114 2307 40-41 0.35438118463041945 2307 42-43 0.304817615695292 2307 44-45 0.27783206670741123 2307 46-47 0.25690430818486476 2307 48-49 0.23075726050232248 2307 50-51 0.24175737974076839 2307 52-53 0.18285898722525928 2307 54-55 0.10542665793686723 2307 56-57 0.1332814744402384 2307 58-59 0.1405454812139837 2307 60-61 -0.009840356673286976 2307 62-63 0.11803582206757568 2307 64-65 0.009420415575583263 2307 66-67 0.002848065796207777 2307 68-69 0.03416341749821328 2307 70-71 0.1620242545303583 2307 72-73 0.0953974433796887 2307 74-75 0.04496344246613404 2307 76-77 -0.20962315145528976 2307 78-79 -0.2486153437511689 2307 80-81 0.06890158740623065 2307 82-83 0.10258708753568158 2307 84-85 0.19309839930685513 2307 86-87 0.10182958606352344 2307 88-89 -0.0871249312639435 2307 90-91 -0.0267223680089117 2307 92-93 -0.05624678941615713 2307 94-95 -0.048568255804223526 2307 96-97 -0.03478072377749797 2307 98-99 -0.15266743818287765 2307 100-101 -0.17424109364351636 2201 1 0.0851095972485254 2201 2 0.10202270241124722 2201 3 0.07979135184849184 2201 4 0.10862400733883248 2201 5 0.04428531760520116 2201 6 0.04493998792528231 2201 7 0.13914280326886086 2201 8 0.10299639639526958 2201 9 0.13590907106139127 2201 10-11 0.15213512602665702 2201 12-13 0.07038320967831169 2201 14-15 0.2565163518521629 2201 16-17 0.1866406258196136 2201 18-19 0.15298712142664073 2201 20-21 0.1966039386733911 2201 22-23 0.2555148542162655 2201 24-25 0.2940592740131791 2201 26-27 0.26388989273545604 2201 28-29 0.17657433021170021 2201 30-31 0.1592644941354493 2201 32-33 0.2882146997086039 2201 34-35 0.34406007536627925 2201 36-37 0.20164276901759592 2201 38-39 0.28082560451427696 2201 40-41 0.37051207613158255 2201 42-43 0.28757398449128146 2201 44-45 0.29789378644971265 2201 46-47 0.2840766778726973 2201 48-49 0.2545863812837652 2201 50-51 0.25490675550162933 2201 52-53 0.19639854336951146 2201 54-55 0.38366377810879726 2201 56-57 0.2330869284561743 2201 58-59 0.33783311737721533 2201 60-61 0.24707314168799144 2201 62-63 0.12501866567451003 2201 64-65 0.275617766372811 2201 66-67 0.34541696000916744 2201 68-69 0.21334312178567671 2201 70-71 0.2144222972298806 2201 72-73 0.08455352905577485 2201 74-75 0.12264586663739152 2201 76-77 -0.04828875763168483 2201 78-79 0.20369862711426379 2201 80-81 0.2250296457294496 2201 82-83 0.3736051285135318 2201 84-85 0.37178353429177946 2201 86-87 0.2464299249234827 2201 88-89 0.24363696602197393 2201 90-91 0.27433667654057814 2201 92-93 0.14719162986185097 2201 94-95 -0.027767980336214748 2201 96-97 0.22184953924629625 2201 98-99 0.05676979835053686 2201 100-101 0.10601338774145219 1101 1 0.07948510475134185 1101 2 0.07373448808514382 1101 3 0.028037284495795234 1101 4 0.07693817948019444 1101 5 0.02585844019633754 1101 6 0.029757538232765057 1101 7 0.12424757698169486 1101 8 0.03038392599274431 1101 9 0.12427831829073455 1101 10-11 0.13644614589114923 1101 12-13 0.1110233767944635 1101 14-15 0.07065647977429279 1101 16-17 0.05726263802301901 1101 18-19 0.0022132338638343185 1101 20-21 -0.0019899531618676747 1101 22-23 0.08013280220847463 1101 24-25 0.09783587284525908 1101 26-27 0.11534542726121089 1101 28-29 0.17126443710068884 1101 30-31 0.1463205918584194 1101 32-33 0.14842067479316512 1101 34-35 0.15486811259521716 1101 36-37 0.030653589561012495 1101 38-39 0.0793264180147446 1101 40-41 0.14245011007540143 1101 42-43 0.16738579930638053 1101 44-45 0.08204898761680113 1101 46-47 0.23782533226096092 1101 48-49 0.12790111291238304 1101 50-51 0.012981622675759752 1101 52-53 0.12902700254159782 1101 54-55 0.0292015231537448 1101 56-57 -0.030171872398078392 1101 58-59 -0.04440394006687143 1101 60-61 0.03160412681041436 1101 62-63 -0.057220097158541705 1101 64-65 -0.04761881655426947 1101 66-67 0.13090834839324827 1101 68-69 -0.047592759777749905 1101 70-71 -0.24678863066328205 1101 72-73 -0.15402111282750042 1101 74-75 -0.07374867180956102 1101 76-77 -0.25080594166948345 1101 78-79 -0.05150550196154313 1101 80-81 0.2561363893161399 1101 82-83 0.30692905727494235 1101 84-85 0.33056712521863574 1101 86-87 0.16773011347367373 1101 88-89 0.0465888447323195 1101 90-91 0.04035237329968311 1101 92-93 -0.06297960914213263 1101 94-95 -0.08321615954925932 1101 96-97 -0.015682421837325933 1101 98-99 -0.002825467968389006 1101 100-101 0.04051672761110936 2304 1 0.037406093018354625 2304 2 0.07736083742850042 2304 3 0.07526174650188722 2304 4 0.08582844880412921 2304 5 0.08303617808589081 2304 6 0.11542009897649308 2304 7 0.052846612475832444 2304 8 -0.05919863773093681 2304 9 0.032253402122755404 2304 10-11 0.050070526340789456 2304 12-13 -0.004974780903559406 2304 14-15 0.08683655709047855 2304 16-17 0.10462125366947106 2304 18-19 0.12160913357964631 2304 20-21 0.053141871694535325 2304 22-23 0.1267201744579438 2304 24-25 0.001257994976604948 2304 26-27 0.06809113777557485 2304 28-29 0.027818976717050248 2304 30-31 0.002781798070493835 2304 32-33 0.05216585793530015 2304 34-35 0.16876918842899613 2304 36-37 0.18081352366608883 2304 38-39 0.10872553013609831 2304 40-41 0.06710512656733414 2304 42-43 0.06877776235503319 2304 44-45 -0.013293570483064343 2304 46-47 0.050203274153680866 2304 48-49 0.07277902049649043 2304 50-51 0.036818890108939684 2304 52-53 0.04568664411332435 2304 54-55 0.03922607691084323 2304 56-57 0.12373911232045032 2304 58-59 0.024515952285334208 2304 60-61 0.043020769647775126 2304 62-63 0.10394071552683926 2304 64-65 -0.10949942373532906 2304 66-67 -0.08946750305526052 2304 68-69 -0.07325839569261205 2304 70-71 0.007305505982422744 2304 72-73 0.10425223179640142 2304 74-75 0.07282538310045794 2304 76-77 -0.3574447824251905 2304 78-79 0.03844675446627832 2304 80-81 0.24335041310474992 2304 82-83 0.24290507262054462 2304 84-85 0.26818603253583007 2304 86-87 0.23872714668972606 2304 88-89 0.11135655588356741 2304 90-91 0.07798372973862655 2304 92-93 0.0245761563416238 2304 94-95 0.17702831935627472 2304 96-97 0.07384503806852294 2304 98-99 0.05088857136411207 2304 100-101 0.21281103213091157 2202 1 0.09954755883313737 2202 2 0.07910151911171681 2202 3 0.06401102755008736 2202 4 0.019265721655628454 2202 5 0.0666700553546491 2202 6 0.09455218191820336 2202 7 0.08725550290264295 2202 8 0.06835860953705009 2202 9 0.14336269474691932 2202 10-11 0.025897249474077455 2202 12-13 0.060915625642572024 2202 14-15 7.671153088182336E-4 2202 16-17 -0.042636701821237466 2202 18-19 -0.06745966861416974 2202 20-21 -0.08710776499775363 2202 22-23 -0.10551827386351675 2202 24-25 -0.1480001662629178 2202 26-27 -0.05630746261295627 2202 28-29 -0.0031134212905143954 2202 30-31 0.005758920985137195 2202 32-33 -0.0059023270121016935 2202 34-35 -0.12357557903068539 2202 36-37 -0.1061358579704148 2202 38-39 -0.01765028513057132 2202 40-41 -0.15024861642434217 2202 42-43 -0.28531392419808554 2202 44-45 -0.11473455252836828 2202 46-47 -0.32773572548106955 2202 48-49 -0.26652648375175403 2202 50-51 -0.09452840868456747 2202 52-53 -0.1445929456568038 2202 54-55 -0.2094646124879489 2202 56-57 -0.0922260859959465 2202 58-59 -0.12683210507701403 2202 60-61 -0.08709275069875844 2202 62-63 -0.45378832160509575 2202 64-65 -0.34575588391020773 2202 66-67 -0.13094641094473758 2202 68-69 -0.2907144935209729 2202 70-71 -0.2478737396780204 2202 72-73 -0.30469904937961445 2202 74-75 -0.3184802749398372 2202 76-77 -0.6263934813497372 2202 78-79 -0.350774462912014 2202 80-81 -0.06939764799849257 2202 82-83 0.0662598485128143 2202 84-85 -0.15776232139576507 2202 86-87 -0.13343568894450897 2202 88-89 -0.08329967734413657 2202 90-91 -0.0860397012058911 2202 92-93 -0.11212567726474276 2202 94-95 -0.2379622219137687 2202 96-97 -0.12858081052841186 2202 98-99 -0.19113340167344006 2202 100-101 -0.24821703907272408 1102 1 0.1301356601084791 1102 2 0.11778359939322058 1102 3 0.05778679241611684 1102 4 0.004450106178850888 1102 5 0.027489860275338174 1102 6 -0.02661349945860536 1102 7 0.08849312018971034 1102 8 0.009247639215608672 1102 9 0.03544194410967805 1102 10-11 0.07957937494145284 1102 12-13 0.024227431474599825 1102 14-15 -0.0210785302236971 1102 16-17 -0.0795449987561554 1102 18-19 -0.04626029997972836 1102 20-21 0.004230364697122013 1102 22-23 5.046700960136263E-4 1102 24-25 0.02183010608423075 1102 26-27 0.04543203892502845 1102 28-29 -0.004182126648657913 1102 30-31 -0.03218722411288155 1102 32-33 0.03081856745890832 1102 34-35 -0.007710795140447146 1102 36-37 -0.011961779631427305 1102 38-39 -0.02464155046344274 1102 40-41 -0.0734163411539015 1102 42-43 -0.034472694038846896 1102 44-45 -0.07663340799397389 1102 46-47 -0.0014930069467240514 1102 48-49 0.04392329643086157 1102 50-51 0.04875165526058112 1102 52-53 -0.08861989641419399 1102 54-55 -0.0624084959141058 1102 56-57 -0.10306344206109941 1102 58-59 -0.07277630432864157 1102 60-61 -0.0011913139306827247 1102 62-63 -0.1005038793866504 1102 64-65 -0.14185576038629222 1102 66-67 -0.049137625193800716 1102 68-69 -0.07061660189382479 1102 70-71 0.023406250067168344 1102 72-73 -0.12608172996733202 1102 74-75 -0.3390781363637956 1102 76-77 -0.40550228037105995 1102 78-79 -0.08454554033824024 1102 80-81 0.03576462398526559 1102 82-83 0.03513320297410871 1102 84-85 0.005121574216310876 1102 86-87 0.08497772089088329 1102 88-89 -0.06916666582934994 1102 90-91 -0.02315981286731983 1102 92-93 -0.0949509430171318 1102 94-95 -0.06570332778807852 1102 96-97 -0.09676775590154563 1102 98-99 0.05966121569020544 1102 100-101 0.025170277109953787 2305 1 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-0.16682802551152776 1213 68-69 -0.13081173653777256 1213 70-71 -0.19128715104773875 1213 72-73 -6.826708123881531E-4 1213 74-75 0.09042434221304774 1213 76-77 0.27482886185912747 1213 78-79 -0.19915437610409725 1213 80-81 -0.4066887569923381 1213 82-83 -0.3639611033496237 1213 84-85 -0.3680930686395314 1213 86-87 -0.3507768561631792 1213 88-89 -0.4328040101784012 1213 90-91 -0.3740003956692348 1213 92-93 -0.29862167714375687 1213 94-95 -0.1812202410599042 1213 96-97 -0.43703286200823044 1213 98-99 -0.42494954734136314 1213 100-101 -0.09836486963092739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 12.0 10 14.0 11 7.0 12 30.0 13 101.0 14 339.0 15 804.0 16 1599.0 17 2600.0 18 3808.0 19 5029.0 20 6636.0 21 8591.0 22 10714.0 23 13505.0 24 17063.0 25 21207.0 26 27302.0 27 36021.0 28 51799.0 29 72949.0 30 73092.0 31 74642.0 32 96652.0 33 135127.0 34 202605.0 35 327289.0 36 583738.0 37 905501.0 38 352273.0 39 1560.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.419931460546884 25.246289923141624 22.0739046451546 16.259873971156892 2 29.067516714963865 24.963134090348518 31.506668179114243 14.462681015573372 3 36.06945150236756 20.177665100164983 26.99463502577292 16.758248371694542 4 28.654546102104394 20.94736553985715 31.76248125639146 18.635607101646997 5 32.53864985036002 19.12536123974976 31.71104645104616 16.624942458844057 6 27.923387495663803 20.332637343649633 35.11580116414497 16.628173996541594 7 31.98071497441809 22.72071072725426 27.146235654280865 18.152338644046782 8 28.44603925592855 30.162843120056245 24.132035354341404 17.2590822696738 9 31.722950380727898 25.125469397338 24.567864270140724 18.583715951793373 10-11 26.761336432092204 28.9654265036213 27.70872106289891 16.564516001387585 12-13 25.582435591783977 27.962332776057586 27.214297255499385 19.240934376659048 14-15 24.5780535063503 28.477447823856135 27.838740992913042 19.105757676880526 16-17 24.831168642740977 27.646728298905366 28.13109622991665 19.391006828437003 18-19 22.546224179024552 28.10024823485497 29.705498098668738 19.64802948745174 20-21 23.61324165438902 27.984374525986176 29.795700867766794 18.606682951858 22-23 22.147850958646814 31.015432921102892 28.071613607825345 18.76510251242495 24-25 21.935391966756036 28.050272199479625 29.80394284066483 20.21039299309951 26-27 22.0143394539097 27.953295706803242 28.197639526586322 21.834725312700733 28-29 22.922929144908746 29.84001909904729 28.065641104104316 19.17141065193965 30-31 21.2463381401907 28.46579120573288 29.63150248037006 20.656368173706362 32-33 21.207229939075624 30.07992779821487 28.16210909935066 20.55073316335885 34-35 22.44365583200225 28.621119351898628 28.60802832673616 20.327196489362965 36-37 21.1595812454742 28.5326147888355 29.80963275222811 20.49817121346219 38-39 21.209822936012156 30.065660349485057 28.172896573737972 20.551620140764815 40-41 21.097209270424308 28.36198630091815 30.94489173029718 19.595912698360358 42-43 21.084513943755418 29.7541855008158 29.546575691186344 19.61472486424244 44-45 20.674627140298526 28.356924410124158 30.05211520049489 20.91633324908243 46-47 22.275940709211532 28.187526194700947 28.354908478888763 21.18162461719876 48-49 20.854117123710825 30.043958613182504 29.388709655346734 19.713214607759937 50-51 22.190802502080313 28.291366820478753 29.4982039318452 20.01962674559573 52-53 20.77018680238465 28.48938340275206 28.019731164694683 22.720698630168606 54-55 21.977261541733753 28.426568266899476 29.747248788345694 19.848921403021077 56-57 20.85744393219183 28.240130599958814 29.449772981828488 21.452652486020867 58-59 20.692118772831336 28.600815683144937 29.670338367528988 21.036727176494736 60-61 21.907128914413356 28.4398305918913 29.7048350427535 19.948205450941835 62-63 20.575042282520773 28.717308744345154 29.279306015972555 21.42834295716152 64-65 21.835590749137566 28.53710585493678 29.560778754455185 20.06652464147047 66-67 20.820563420087122 30.89510778007394 27.9150811967792 20.369247603059733 68-69 20.807628084073414 31.15147260058829 27.935740765443306 20.10515854989499 70-71 20.585645554951327 31.080417065562475 28.12359740648195 20.21033997300425 72-73 20.484166378666725 31.273298316057733 27.864225394471987 20.37830991080356 74-75 20.54796869664266 30.810583886662602 28.239538189571604 20.401909227123134 76-77 20.935362341446297 30.21368361989751 28.16637897304507 20.68457506561113 78-79 20.74062544518253 30.240210601016205 28.465185406982574 20.553978546818694 80-81 20.455146510845694 30.144812599339165 28.59095016059145 20.809090729223687 82-83 20.38681506239506 30.250589261006684 28.259516878519243 21.103078798079018 84-85 20.291670850375247 30.034872756728433 28.691650867499185 20.98180552539713 86-87 20.505648776223516 29.731518870445907 28.66888514471027 21.09394720862031 88-89 20.550347532809717 30.230515120212303 28.458942817641525 20.760194529336452 90-91 20.72117972854767 29.667927222481914 28.435979181454908 21.174913867515503 92-93 20.51555683363272 29.95940749251484 28.201619017436407 21.323416656416033 94-95 20.20486061243829 30.025941887731133 28.529827234909376 21.239370264921202 96-97 20.500974097980322 30.038048770031256 28.141991578978608 21.31898555300981 98-99 20.523592289023288 30.000740342935423 28.090853434028766 21.384813934012527 100-101 20.545370033420927 29.667012544430836 28.066405892530344 21.721211529617893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74.0 1 46.5 2 34.0 3 90.5 4 95.5 5 53.5 6 48.5 7 63.0 8 76.5 9 66.0 10 64.0 11 68.0 12 60.5 13 65.5 14 89.0 15 130.5 16 170.5 17 241.5 18 376.5 19 620.0 20 994.0 21 1537.0 22 2395.0 23 3659.0 24 5398.0 25 7613.5 26 10403.0 27 13991.5 28 18413.5 29 23361.0 30 28904.0 31 35713.0 32 44276.0 33 54832.0 34 67809.5 35 84734.0 36 105414.0 37 129691.5 38 153825.0 39 170300.0 40 179256.0 41 176594.5 42 167491.5 43 163625.0 44 157493.5 45 151344.5 46 149032.0 47 139711.5 48 122697.0 49 106917.5 50 95435.0 51 84996.5 52 73767.5 53 62533.5 54 51621.5 55 41465.0 56 32817.0 57 25799.0 58 20371.0 59 16016.0 60 12472.5 61 9530.5 62 7040.5 63 5155.0 64 3707.5 65 2650.0 66 1980.5 67 1467.5 68 1036.0 69 738.5 70 534.5 71 391.0 72 295.0 73 205.0 74 160.5 75 125.0 76 83.0 77 66.0 78 59.0 79 45.5 80 35.0 81 24.5 82 14.5 83 10.0 84 9.0 85 8.0 86 5.0 87 4.5 88 4.0 89 2.5 90 2.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1508600506757871 2 0.0 3 0.0054408542866677305 4 0.008078844243839964 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.594974892930582E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3189949785861164E-4 24-25 4.946231169697937E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0011870954807275049 40-41 0.0 42-43 0.0 44-45 1.154120606262852E-4 46-47 0.0010881708573335462 48-49 0.0033139748836976176 50-51 5.111105542021201E-4 52-53 0.0016157688487679928 54-55 0.002786376892263171 56-57 0.0047978442346069995 58-59 0.006083864338728462 60-61 0.001764155783858931 62-63 0.001154120606262852 64-65 0.001698206034929625 66-67 0.008458055300183473 68-69 0.00786450755981972 70-71 0.009331889473496774 72-73 0.006726874390789194 74-75 0.009414326659658407 76-77 0.005325442226041446 78-79 0.004880281420768631 80-81 0.0023741909614550098 82-83 0.0 84-85 5.111105542021201E-4 86-87 0.001731180909394278 88-89 0.005424366849435405 90-91 0.007237984944991314 92-93 0.009199989975638163 94-95 0.008491030174648124 96-97 0.010535472391456606 98-99 0.007699633187496456 100-101 0.007600708564102496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3032612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.23544309818942 #Duplication Level Percentage of deduplicated Percentage of total 1 94.71854315765094 74.103471935538 2 3.214490183491033 5.029741276804023 3 0.7678719729903399 1.8022441214874052 4 0.35518881079605535 1.111534159845934 5 0.20193572837693013 0.7899265593462376 6 0.13276816789908585 0.6232305866951874 7 0.07889485547123946 0.4320661783172023 8 0.07488949796403199 0.4687210445293598 9 0.05298674953219908 0.3730897645185938 >10 0.3349008969957949 4.898618889640468 >50 0.0335394655112836 1.8669080736310657 >100 0.031885056917859624 4.804185871652324 >500 0.00179990917303696 0.9782637576984298 >1k 2.632723837617767E-4 0.25700095940816775 >5k 0.0 0.0 >10k+ 4.2274846377639146E-5 2.4609968208877335 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 74409 2.4536274340403588 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.254472382223641E-4 0.0 3.297487446465291E-5 2 8.243718616163228E-4 0.0 3.297487446465291E-5 3 0.0010222211084042403 0.0 3.297487446465291E-5 4 0.0011870954807275049 0.0 3.297487446465291E-5 5 0.0016157688487679928 0.0 3.297487446465291E-5 6 0.0019784924678791747 0.0 6.594974892930582E-5 7 0.00260501508270758 0.0 6.594974892930582E-5 8 0.0030666633252127208 0.0 6.594974892930582E-5 9 0.0032645125720006385 0.0 6.594974892930582E-5 10-11 0.005028668355859569 0.0 1.3189949785861164E-4 12-13 0.00651253770676895 0.0 1.3189949785861164E-4 14-15 0.008161281430001596 0.0 1.4838693509093808E-4 16-17 0.009480276408587712 0.0 1.6487437232326455E-4 18-19 0.01111253269458803 0.0 1.8136180955559102E-4 20-21 0.012942638227376268 0.0 2.4731155848489684E-4 22-23 0.014706794011235199 0.0 2.967738701818762E-4 24-25 0.016339050297235518 0.0 2.967738701818762E-4 26-27 0.01856485432359959 0.0 2.967738701818762E-4 28-29 0.020757683475499007 0.0 2.967738701818762E-4 30-31 0.03513472874208768 0.0 2.967738701818762E-4 32-33 0.03833329156515901 0.0 2.967738701818762E-4 34-35 0.041202105643583814 0.0 3.462361818788556E-4 36-37 0.044186331782634904 0.0 3.95698493575835E-4 38-39 0.047582743852494155 0.0 3.95698493575835E-4 40-41 0.05168811572334344 0.0 3.95698493575835E-4 42-43 0.05561212578463714 0.0 3.95698493575835E-4 44-45 0.05928882428744593 0.0 3.95698493575835E-4 46-47 0.0640701810848206 0.0 3.95698493575835E-4 48-49 0.0704178444192663 0.0 4.2867336804048786E-4 50-51 0.07674902031647965 0.0 4.4516080527281436E-4 52-53 0.08339345752110722 0.0 4.616482425051408E-4 54-55 0.09175258819789672 0.0 4.7813567973746724E-4 56-57 0.10073824148951466 0.0 4.946231169697937E-4 58-59 0.11072962845230447 0.0 4.946231169697937E-4 60-61 0.12180918627242786 0.0 4.946231169697937E-4 62-63 0.13440558831792526 0.0 4.946231169697937E-4 64-65 0.14830449790477646 0.0 4.946231169697937E-4 66-67 0.16452813614138573 0.0 4.946231169697937E-4 68-69 0.18332381458623787 0.0 4.946231169697937E-4 70-71 0.20352092519583778 0.0 5.111105542021201E-4 72-73 0.22635602576260994 0.0 5.275979914344466E-4 74-75 0.25374165900550416 0.0 5.605728658990996E-4 76-77 0.28409503095021715 0.0 5.605728658990996E-4 78-79 0.3167401566702236 0.0 5.77060303131426E-4 80-81 0.3534082170749176 0.0 6.100351775960789E-4 82-83 0.3954017197056531 0.0 6.594974892930582E-4 84-85 0.4426217399390361 0.0 6.594974892930582E-4 86-87 0.4952826144590868 0.0 6.924723637577112E-4 88-89 0.5535162427636637 0.0 7.749095499193434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 10715 0.0 73.497894 3 CGGAAGA 10770 0.0 73.034355 4 GATCGGA 10865 0.0 72.50336 1 ATCGGAA 11035 0.0 71.40959 2 GAAGAGC 11870 0.0 66.222916 6 GGAAGAG 15150 0.0 52.293106 5 AGAGCAC 15485 0.0 50.88575 8 TTGTGTT 22660 0.0 50.466362 1 GAGCACA 15670 0.0 50.315308 9 CGACGTC 40 5.350942E-4 47.569378 1 AAGAGCA 16770 0.0 46.78838 7 GCCGTCT 8790 0.0 44.9335 50-51 ATGCCGT 8745 0.0 44.729443 48-49 TCGTATG 8780 0.0 44.6045 44-45 TGCCGTC 8775 0.0 44.60432 50-51 TATGCCG 8730 0.0 44.588657 48-49 CGTATGC 8715 0.0 44.55566 46-47 TCTCGTA 8715 0.0 44.473907 42-43 CGTCTTC 8880 0.0 44.291237 52-53 CTCGTAT 8725 0.0 44.0963 44-45 >>END_MODULE