FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032612
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT744092.4536274340403588TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG107150.073.4978943
CGGAAGA107700.073.0343554
GATCGGA108650.072.503361
ATCGGAA110350.071.409592
GAAGAGC118700.066.2229166
GGAAGAG151500.052.2931065
AGAGCAC154850.050.885758
TTGTGTT226600.050.4663621
GAGCACA156700.050.3153089
CGACGTC405.350942E-447.5693781
AAGAGCA167700.046.788387
GCCGTCT87900.044.933550-51
ATGCCGT87450.044.72944348-49
TCGTATG87800.044.604544-45
TGCCGTC87750.044.6043250-51
TATGCCG87300.044.58865748-49
CGTATGC87150.044.5556646-47
TCTCGTA87150.044.47390742-43
CGTCTTC88800.044.29123752-53
CTCGTAT87250.044.096344-45
Produced by FastQC (version 0.11.2)