##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448160 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69050062087445 33.0 33.0 33.0 33.0 33.0 2 32.72661590745703 33.0 33.0 33.0 33.0 33.0 3 32.75621732239723 33.0 33.0 33.0 33.0 33.0 4 36.70296018234102 37.0 37.0 37.0 37.0 37.0 5 36.700258561531925 37.0 37.0 37.0 37.0 37.0 6 36.5878855957127 37.0 37.0 37.0 37.0 37.0 7 36.5359045160447 37.0 37.0 37.0 37.0 37.0 8 36.46515260440494 37.0 37.0 37.0 37.0 37.0 9 36.47778331481602 37.0 37.0 37.0 37.0 37.0 10-11 36.4298242353441 37.0 37.0 37.0 37.0 37.0 12-13 36.421913804653286 37.0 37.0 37.0 37.0 37.0 14-15 38.288520153911506 40.0 37.0 40.0 37.0 40.0 16-17 38.27600994216064 40.0 37.0 40.0 37.0 40.0 18-19 38.31243260244429 40.0 37.0 40.0 37.0 40.0 20-21 38.270695338539966 40.0 37.0 40.0 37.0 40.0 22-23 38.18514435331024 40.0 37.0 40.0 37.0 40.0 24-25 38.16059714234364 40.0 37.0 40.0 37.0 40.0 26-27 38.08308586857068 40.0 37.0 40.0 37.0 40.0 28-29 37.97793792072413 40.0 37.0 40.0 35.0 40.0 30-31 37.949892368145875 40.0 37.0 40.0 35.0 40.0 32-33 37.820464757205414 40.0 37.0 40.0 33.0 40.0 34-35 37.75582110646363 40.0 37.0 40.0 33.0 40.0 36-37 37.67515848637344 40.0 37.0 40.0 33.0 40.0 38-39 37.57510640644402 40.0 37.0 40.0 33.0 40.0 40-41 37.518681785504214 40.0 37.0 40.0 33.0 40.0 42-43 37.405657718449774 40.0 37.0 40.0 33.0 40.0 44-45 37.26925139696752 40.0 37.0 40.0 33.0 40.0 46-47 37.234913976210706 40.0 37.0 40.0 33.0 40.0 48-49 37.05838078393569 38.5 37.0 40.0 33.0 40.0 50-51 36.914718809228155 37.0 37.0 40.0 33.0 40.0 52-53 36.78533510881642 37.0 37.0 40.0 33.0 40.0 54-55 36.62736871773087 37.0 37.0 40.0 33.0 40.0 56-57 36.46602652604405 37.0 37.0 40.0 33.0 40.0 58-59 36.26078524279459 37.0 37.0 40.0 33.0 40.0 60-61 36.00709063133128 37.0 37.0 40.0 33.0 40.0 62-63 35.769838572642314 37.0 37.0 40.0 30.0 40.0 64-65 35.5212098065486 37.0 33.0 40.0 27.0 40.0 66-67 35.22632140056206 37.0 33.0 38.5 27.0 40.0 68-69 34.975256519181755 37.0 33.0 37.0 27.0 40.0 70-71 34.64204913077577 37.0 33.0 37.0 27.0 40.0 72-73 34.34717849323574 37.0 33.0 37.0 27.0 40.0 74-75 34.00663886347297 37.0 33.0 37.0 27.0 38.5 76-77 31.288688443565782 33.0 30.0 35.0 24.5 37.0 78-79 33.718934424220635 37.0 33.0 37.0 27.0 37.0 80-81 34.09435433141624 37.0 33.0 37.0 27.0 37.0 82-83 34.02408441441736 37.0 33.0 37.0 27.0 37.0 84-85 33.843952192667146 37.0 33.0 37.0 27.0 37.0 86-87 33.67315187732828 37.0 33.0 37.0 27.0 37.0 88-89 33.534387254101034 37.0 33.0 37.0 27.0 37.0 90-91 33.38635567119796 37.0 33.0 37.0 27.0 37.0 92-93 33.250286133259266 37.0 33.0 37.0 27.0 37.0 94-95 33.08375494248742 37.0 33.0 37.0 27.0 37.0 96-97 32.92848077740017 33.0 33.0 37.0 27.0 37.0 98-99 32.80129995588524 33.0 33.0 37.0 27.0 37.0 100-101 31.263232591007124 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15321006086630007 2306 2 0.14547908331598336 2306 3 0.08115189624503927 2306 4 0.09217502238966802 2306 5 0.09625978069335872 2306 6 0.1361618232551436 2306 7 0.03679826692150101 2306 8 0.12270090432225089 2306 9 0.09436810220622505 2306 10-11 0.09658750625463597 2306 12-13 0.15858585873017716 2306 14-15 0.3776286249864853 2306 16-17 0.3203409979188052 2306 18-19 0.38051733608970295 2306 20-21 0.3198278641839991 2306 22-23 0.3285581638306425 2306 24-25 0.3787913385685542 2306 26-27 0.3488783386885075 2306 28-29 0.3485805710008023 2306 30-31 0.3880299968527865 2306 32-33 0.4753517368366573 2306 34-35 0.4655279643826944 2306 36-37 0.31944973257218834 2306 38-39 0.3314497981781628 2306 40-41 0.40052038200440876 2306 42-43 0.38254774835755256 2306 44-45 0.36327809150611046 2306 46-47 0.2722148741727679 2306 48-49 0.2983968240355068 2306 50-51 0.511314682556204 2306 52-53 0.4818006519226259 2306 54-55 0.33074270384105375 2306 56-57 0.3527217289982687 2306 58-59 0.32928780413286773 2306 60-61 0.19064474689816535 2306 62-63 0.171956745899557 2306 64-65 0.24079816793651077 2306 66-67 0.30024378575812705 2306 68-69 0.468104254175735 2306 70-71 0.5176243125170288 2306 72-73 0.38899814527487564 2306 74-75 0.31133977714313943 2306 76-77 -0.04601604021151928 2306 78-79 0.3176980129309612 2306 80-81 0.3746252542237727 2306 82-83 0.4808131927954804 2306 84-85 0.6338982021618378 2306 86-87 0.5800960489888638 2306 88-89 0.5185270350973923 2306 90-91 0.46847557183250643 2306 92-93 0.3627033879452455 2306 94-95 0.25722097137878563 2306 96-97 0.511030672693984 2306 98-99 0.5168055129551377 2306 100-101 0.5331952474947705 2307 1 0.051147936756727574 2307 2 0.0536793913833975 2307 3 0.04269165841429157 2307 4 0.08550642818816812 2307 5 0.08038044437403613 2307 6 0.05734509292910417 2307 7 0.12650725988599731 2307 8 0.09232870410887273 2307 9 0.09918059871890961 2307 10-11 0.12758383041189347 2307 12-13 0.17826024060864398 2307 14-15 0.27538241284605647 2307 16-17 0.3219982129015051 2307 18-19 0.2866237749014644 2307 20-21 0.3563290756565536 2307 22-23 0.3228457283565618 2307 24-25 0.35014631101664406 2307 26-27 0.4457185764854401 2307 28-29 0.3987691819752399 2307 30-31 0.45368876569909133 2307 32-33 0.4058313463925032 2307 34-35 0.3444124120509642 2307 36-37 0.38516514388952316 2307 38-39 0.34173611318808383 2307 40-41 0.32721340010125033 2307 42-43 0.17841737091579546 2307 44-45 0.260941843199042 2307 46-47 0.11825810167767514 2307 48-49 0.2204675628731394 2307 50-51 0.26279119114810356 2307 52-53 0.32224071319794945 2307 54-55 0.26783743541492555 2307 56-57 0.29602007926516904 2307 58-59 0.1503305666622694 2307 60-61 0.1865605711137519 2307 62-63 0.31337672192372423 2307 64-65 0.19988962270115707 2307 66-67 0.22189118553053788 2307 68-69 0.2505576424945346 2307 70-71 0.38653186524102523 2307 72-73 0.2940774109179145 2307 74-75 0.3311088449433299 2307 76-77 -0.10049087996404893 2307 78-79 0.03965956706509388 2307 80-81 0.2544115430259808 2307 82-83 0.22496232143137718 2307 84-85 0.3947264249916316 2307 86-87 0.34032403070771267 2307 88-89 0.19300423354385998 2307 90-91 0.24485743677318084 2307 92-93 0.2156838386840505 2307 94-95 0.3192649783517254 2307 96-97 0.3347990371130436 2307 98-99 0.28204825553285673 2307 100-101 0.3499534720661046 2201 1 0.1193784291576847 2201 2 0.07976794493186645 2201 3 0.1077377323055515 2201 4 0.09618591752764871 2201 5 0.1026949003894444 2201 6 0.05190744788096424 2201 7 0.15332057980166525 2201 8 0.15604743100931273 2201 9 0.15508512991269896 2201 10-11 0.17616727264991283 2201 12-13 0.12182712326328016 2201 14-15 0.26292231294213764 2201 16-17 0.21340373600068574 2201 18-19 0.17316311795197947 2201 20-21 0.144972214357864 2201 22-23 0.3574334008016251 2201 24-25 0.37132163162141296 2201 26-27 0.3214699049708045 2201 28-29 0.2787789871914299 2201 30-31 0.30341016702536905 2201 32-33 0.39966039715346824 2201 34-35 0.2407674064122105 2201 36-37 0.24092752980522647 2201 38-39 0.2906691129012131 2201 40-41 0.15709346834312754 2201 42-43 0.27832299950452466 2201 44-45 0.15904064061812306 2201 46-47 0.04084622340199218 2201 48-49 0.2947832468173246 2201 50-51 0.13317488473965255 2201 52-53 0.04814299040628356 2201 54-55 0.18256241124338857 2201 56-57 0.2584771814443201 2201 58-59 0.19393873443771525 2201 60-61 -0.042961933071673286 2201 62-63 0.04880986659013331 2201 64-65 0.00423989052320195 2201 66-67 0.09622049204676131 2201 68-69 0.13066364052438217 2201 70-71 0.2516150881518371 2201 72-73 0.1414998609166318 2201 74-75 0.03429690625780779 2201 76-77 0.07984465271266927 2201 78-79 0.03592872783876544 2201 80-81 0.06595084813061192 2201 82-83 0.13714814849225831 2201 84-85 0.23835314830390786 2201 86-87 0.24176282475718835 2201 88-89 0.2492022761434285 2201 90-91 0.19074471017933803 2201 92-93 0.13715122875102992 2201 94-95 0.004195567742357298 2201 96-97 0.1897706308249596 2201 98-99 0.08483249675938964 2201 100-101 -0.08526986981264173 1101 1 0.12075092087381023 1101 2 0.09321361756821034 1101 3 0.08326238855683954 1101 4 0.06823643380494104 1101 5 0.08256946465908754 1101 6 0.03962816564666838 1101 7 0.11885614902001151 1101 8 0.03556885985706515 1101 9 0.05796102343828835 1101 10-11 0.05063450562924743 1101 12-13 0.018651310065187943 1101 14-15 0.10870164085029899 1101 16-17 0.12490191375948712 1101 18-19 0.08961179076028714 1101 20-21 0.06932604285231037 1101 22-23 0.12071287580891976 1101 24-25 -0.054175181447625675 1101 26-27 0.04403478056070753 1101 28-29 0.08544132594722242 1101 30-31 0.15216057215749146 1101 32-33 0.014371219780528577 1101 34-35 0.10608718690455277 1101 36-37 0.08763398854578952 1101 38-39 0.06595236404866256 1101 40-41 0.14831075666749172 1101 42-43 -0.04585973889277284 1101 44-45 -0.049037978256016856 1101 46-47 0.08234751868698709 1101 48-49 -0.06549452834174474 1101 50-51 -0.04418261034268056 1101 52-53 -0.02138174451459207 1101 54-55 -5.968033220469238E-4 1101 56-57 -0.07030577729283038 1101 58-59 -0.09187600128610995 1101 60-61 -0.035471255580247885 1101 62-63 -0.0627123996661112 1101 64-65 -0.044212447897756135 1101 66-67 -0.1675483923112253 1101 68-69 -0.21341275089517353 1101 70-71 -0.1465622480504436 1101 72-73 -0.2927437423845092 1101 74-75 -0.12888527202503042 1101 76-77 -0.14498701225111432 1101 78-79 -0.20290010957102567 1101 80-81 -0.07179130872288653 1101 82-83 -0.09275672957475933 1101 84-85 -0.008020817654262657 1101 86-87 -0.1515004254605259 1101 88-89 -0.15736247331506803 1101 90-91 -0.3086480386302952 1101 92-93 -0.2596953886355351 1101 94-95 -0.3940514983209198 1101 96-97 -0.21130415176904194 1101 98-99 -0.24875873411731675 1101 100-101 -0.43029500994690295 2304 1 0.08680692497046039 2304 2 0.09879161897737276 2304 3 0.08022198426578342 2304 4 0.08628234867211404 2304 5 0.10458319485416467 2304 6 0.1287877081673372 2304 7 0.0760601400958123 2304 8 0.09418950888938582 2304 9 0.1092052456048691 2304 10-11 0.06873192940670947 2304 12-13 0.051541190531928294 2304 14-15 0.15800644601753078 2304 16-17 0.09696591213927519 2304 18-19 0.10987584724112054 2304 20-21 0.125767902577131 2304 22-23 0.138896398421835 2304 24-25 0.07649039137532299 2304 26-27 0.1232924557016517 2304 28-29 0.06801990962012638 2304 30-31 0.08034461863231712 2304 32-33 0.17520960938389862 2304 34-35 0.25684311462765663 2304 36-37 0.12297423936285412 2304 38-39 0.1429042948307213 2304 40-41 0.09324748091635371 2304 42-43 0.14478025242465264 2304 44-45 0.19477750604930577 2304 46-47 0.0870422237464652 2304 48-49 0.050484362780451875 2304 50-51 0.06895501920974567 2304 52-53 0.18792089000950796 2304 54-55 0.14133079795937675 2304 56-57 0.09544112321175646 2304 58-59 0.12384394163550638 2304 60-61 -0.048599510761654585 2304 62-63 0.09919414186523312 2304 64-65 -0.015052639686359726 2304 66-67 -0.10760818776444836 2304 68-69 -0.01880043252047159 2304 70-71 -0.005270675284151594 2304 72-73 0.0174973067830706 2304 74-75 0.0535768338346827 2304 76-77 -0.26009351036960027 2304 78-79 0.055134089838887235 2304 80-81 0.25129776710198826 2304 82-83 0.21366188221122684 2304 84-85 0.21288322763748369 2304 86-87 0.22748509213884915 2304 88-89 0.15951984148282605 2304 90-91 0.017585587238556855 2304 92-93 0.14945454891461196 2304 94-95 0.2781978068100983 2304 96-97 0.1795183891316654 2304 98-99 -0.0782676248560108 2304 100-101 -0.15659972061847327 2202 1 0.08722611722076579 2202 2 0.08339011160707344 2202 3 0.07292662635087765 2202 4 0.08864298159723205 2202 5 0.11268448794857022 2202 6 0.09680548068259043 2202 7 0.05210260764814478 2202 8 0.03358057861547081 2202 9 0.027957443384146075 2202 10-11 0.10964202469955353 2202 12-13 0.11436119988704974 2202 14-15 0.006421980986530684 2202 16-17 -0.08749633749047803 2202 18-19 -0.03664012212936285 2202 20-21 -0.034393889296460145 2202 22-23 -0.04034460092013603 2202 24-25 -0.11079528030685992 2202 26-27 0.08112732221022156 2202 28-29 0.12460214403623127 2202 30-31 0.023089336732702748 2202 32-33 -0.023720843332696973 2202 34-35 -0.03236620578864091 2202 36-37 -0.14993823731281708 2202 38-39 0.08231765966858973 2202 40-41 -0.011779603229591373 2202 42-43 -0.20648806350634885 2202 44-45 -0.09022079753550116 2202 46-47 -0.03577525588606534 2202 48-49 -0.16335699447532193 2202 50-51 0.09807133148347447 2202 52-53 0.15776134309916046 2202 54-55 0.05839684513097865 2202 56-57 -0.06091835875580642 2202 58-59 -0.21482443535320783 2202 60-61 -0.13260969940699852 2202 62-63 -0.06865847022785232 2202 64-65 -0.04282981041901479 2202 66-67 -0.07900411120260031 2202 68-69 0.07131000775058993 2202 70-71 -0.036896843211366104 2202 72-73 -0.10923726946963797 2202 74-75 -0.10626002334040408 2202 76-77 -0.503407349828457 2202 78-79 -0.15623935306277303 2202 80-81 0.008212618096692381 2202 82-83 0.07071136477700435 2202 84-85 0.13570185513716382 2202 86-87 0.024259188132774057 2202 88-89 0.14612412157035237 2202 90-91 0.1132838226797972 2202 92-93 -0.024318187960467696 2202 94-95 -0.004343950720183898 2202 96-97 0.045049726897978815 2202 98-99 -0.007055934094069016 2202 100-101 -0.05281895529571656 1102 1 0.05172706230439417 1102 2 0.07271807566245059 1102 3 0.07899460372806288 1102 4 0.04171516617390125 1102 5 0.04331368530878876 1102 6 0.03766299635297088 1102 7 0.056206430586932754 1102 8 0.04315369923288159 1102 9 0.07964859535856306 1102 10-11 0.08613917095188128 1102 12-13 0.002097658329780927 1102 14-15 -0.0025067317370286446 1102 16-17 -0.03339351132359525 1102 18-19 -0.030866552121963764 1102 20-21 -0.08404782447997405 1102 22-23 -0.10779050343748509 1102 24-25 0.0016865234680025765 1102 26-27 0.0017734893551022424 1102 28-29 -0.0724902826142042 1102 30-31 0.029847861239005624 1102 32-33 0.03460819292041606 1102 34-35 -0.13260970585964316 1102 36-37 -0.12130766009146754 1102 38-39 0.025059176985600118 1102 40-41 0.04144041186790304 1102 42-43 -0.1279636065260945 1102 44-45 -0.1932240198832531 1102 46-47 -0.15129351458926976 1102 48-49 -0.20696699811918506 1102 50-51 -0.10840976160331905 1102 52-53 -0.08847193810746035 1102 54-55 -0.22147625450284636 1102 56-57 -0.3388442593494858 1102 58-59 -0.2583441129663484 1102 60-61 -0.12389267226171086 1102 62-63 -0.22270299037672459 1102 64-65 -0.15018060240053188 1102 66-67 -0.16938357712604812 1102 68-69 -0.09067683198308174 1102 70-71 -0.20395704638430345 1102 72-73 -0.23536796332357568 1102 74-75 -0.2588495647986164 1102 76-77 -0.5879119243949127 1102 78-79 -0.1664993214496917 1102 80-81 -0.12515145417481932 1102 82-83 -0.10713226616912408 1102 84-85 -0.052653917210001566 1102 86-87 0.0525916586247277 1102 88-89 -0.019155250985363637 1102 90-91 -0.09359186876648806 1102 92-93 -0.09762212919655155 1102 94-95 -0.268409481176505 1102 96-97 -0.03638448194362098 1102 98-99 -0.1406401182629935 1102 100-101 -0.2737233377420907 2305 1 -0.032876294992426836 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-0.1534954588563764 1213 70-71 -0.24169730335164274 1213 72-73 -0.10253911820691286 1213 74-75 0.13795518911086901 1213 76-77 0.26394087744187544 1213 78-79 -0.18899748091806856 1213 80-81 -0.33705591567893833 1213 82-83 -0.35112575533182167 1213 84-85 -0.3347938974390203 1213 86-87 -0.25669252361771555 1213 88-89 -0.39555471359172145 1213 90-91 -0.287764835758928 1213 92-93 -0.2886013752094243 1213 94-95 -0.13822947649025252 1213 96-97 -0.2905650190322362 1213 98-99 -0.3163003179970616 1213 100-101 0.006160864742732031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 20.0 10 11.0 11 15.0 12 17.0 13 34.0 14 133.0 15 306.0 16 718.0 17 1220.0 18 1948.0 19 2678.0 20 3630.0 21 4639.0 22 6198.0 23 7953.0 24 9942.0 25 12717.0 26 16548.0 27 21486.0 28 28747.0 29 35595.0 30 40263.0 31 49964.0 32 67346.0 33 96205.0 34 147659.0 35 257082.0 36 515065.0 37 839233.0 38 280363.0 39 417.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.07098427535556 25.949018048402905 23.595926222305135 13.384071453936397 2 31.428705640154238 22.73454349388929 33.75098032808313 12.08577053787334 3 38.56424262427942 18.845096796120313 27.06904975098365 15.52161082861662 4 30.67383991323476 18.949952716213538 34.62639681207197 15.74981055847973 5 30.681573099797397 17.419449709169335 36.495408796810665 15.4035683942226 6 25.575370890791447 18.28140317626299 40.704488268740604 15.438737664204954 7 34.15344585321221 19.043935036925692 29.711456767531537 17.091162342330566 8 30.244796091758708 28.683255996340108 24.786002548853016 16.285945363048164 9 29.85572838376577 26.634656231618848 25.733938958238024 17.77567642637736 10-11 24.177872361283576 29.01031795307496 30.8890554538919 15.922754231749558 12-13 24.20186932379364 27.746064592236287 30.02933225715007 18.02273382682 14-15 22.909879256257764 28.43163845500294 30.972996046010064 17.685486242729233 16-17 21.755138911496573 29.13310999548845 32.019094344138935 17.092656748876042 18-19 20.46387899483694 29.329476014639567 32.38918207960264 17.817462910920856 20-21 21.036104666361677 29.348735376772762 31.883945493758574 17.731214463106987 22-23 20.340733935922806 30.139944076287605 31.676528849360736 17.842793138428856 24-25 20.34141967845239 29.197785275472192 31.833274785961702 18.627520260113716 26-27 20.263238513822625 29.354065910724792 31.04991095353245 19.332784621920133 28-29 20.370523168420366 29.948798281158094 30.77887066204823 18.90180788837331 30-31 19.3908077903405 30.201212339062806 30.923223972289392 19.4847558983073 32-33 19.20423910201948 30.860748480491473 31.149352983465135 18.78565943402392 34-35 19.803587183844193 30.055511077707344 31.742022580223516 18.398879158224954 36-37 20.169943957911247 30.299102182863862 31.41218302725312 18.118770831971766 38-39 20.642495542026474 30.561570889591145 30.67565743238289 18.12027613599949 40-41 20.38308362198549 30.22870237239396 30.60171720802562 18.78649679759493 42-43 19.805302757989672 30.661088327560286 31.316846121168552 18.216762793281486 44-45 19.534384056565045 30.209484285104498 31.535503266745774 18.720628391584686 46-47 20.28810070678234 29.84277432186189 30.89978216088397 18.9693428104718 48-49 19.963616771496007 30.775260548358606 30.61742424551878 18.643698434626604 50-51 20.45629213774584 30.17876993880881 30.217207181024502 19.147730742420848 52-53 19.91496761196115 29.925992371307686 29.988182797780834 20.17085721895033 54-55 20.642045657623985 29.989941230233498 30.28132898304306 19.08668412909946 56-57 20.131580721046994 30.31916045192986 30.153111888954566 19.396146938068583 58-59 20.147448450639327 30.322784494710465 30.61697879366449 18.912788260985717 60-61 20.38812584414144 30.692977703126495 30.318793149120133 18.600103303611927 62-63 19.848354415643694 30.62101164454275 30.2217293315238 19.308904608289755 64-65 20.3784108907504 30.223364135723862 30.319498638247726 19.078726335278013 66-67 20.367522977079496 30.441387869115104 29.90837763170741 19.28271152209799 68-69 20.370151370599288 30.7569224409247 29.978939938075943 18.893986250400072 70-71 19.7211147240968 31.4310282847759 30.031801990228598 18.816055000898707 72-73 19.58653456513356 31.398222070443982 29.979481343259195 19.03576202116326 74-75 20.101574579598072 30.65174809177456 29.795768304564422 19.45090902406294 76-77 20.093563978787262 30.287442186304858 30.046250956115205 19.572742878792678 78-79 19.85733210297284 30.450441839119335 30.365333735283773 19.32689232262405 80-81 19.29819688353013 30.752546083784615 30.229492192486283 19.71976484019897 82-83 18.742504574864387 30.678468727534145 30.132752761257436 20.44627393634403 84-85 18.82046899884852 30.50563466060713 30.568049111349747 20.105847229194605 86-87 18.983651791011702 30.396088439021252 31.17313130137217 19.447128468594876 88-89 19.154778839957803 30.784509759268317 30.75326049326864 19.307450907505235 90-91 19.425662254732856 30.561827632500478 30.526635919362572 19.485874193404097 92-93 18.788473220044864 31.47934062890502 30.436312714744346 19.295873436305772 94-95 18.651212038645692 31.676057390921425 30.338486827727152 19.334243742705727 96-97 19.116889515087664 31.727988620575697 30.03802809479204 19.1170937695446 98-99 19.382009677698058 31.013670550177807 29.795002000613152 19.809317771510983 100-101 19.22983440400064 30.985034350406004 29.51432717076674 20.270804074826614 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 129.0 1 92.5 2 77.0 3 184.0 4 223.5 5 155.5 6 129.5 7 135.5 8 145.0 9 140.5 10 146.5 11 160.0 12 150.0 13 136.0 14 152.0 15 176.0 16 217.5 17 297.0 18 420.0 19 618.5 20 970.5 21 1503.5 22 2337.0 23 3715.0 24 5568.0 25 8024.5 26 11842.0 27 17082.5 28 22603.0 29 28382.5 30 34716.0 31 41388.5 32 49966.5 33 62280.0 34 77892.5 35 96917.0 36 124565.5 37 154781.0 38 179341.5 39 188592.0 40 181153.5 41 164470.0 42 146756.5 43 136675.5 44 122711.5 45 106982.5 46 99962.0 47 89375.5 48 69700.0 49 51866.5 50 39163.0 51 31198.5 52 25680.0 53 19724.5 54 14362.0 55 10056.5 56 6861.5 57 4650.5 58 3269.0 59 2375.5 60 1599.5 61 1025.5 62 709.0 63 484.5 64 319.5 65 213.0 66 142.5 67 91.0 68 67.5 69 56.0 70 36.0 71 28.0 72 26.5 73 17.0 74 13.0 75 12.5 76 8.0 77 6.0 78 4.5 79 3.5 80 2.5 81 2.0 82 1.5 83 2.0 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15701588131494673 2 0.0 3 0.005228416443369715 4 0.0071482256061695315 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.169400692765179E-5 14-15 0.0 16-17 2.0423501731912948E-5 18-19 0.0 20-21 0.0 22-23 1.4296451212339063E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.55669564080779E-4 40-41 0.0 42-43 0.0 44-45 2.042350173191295E-4 46-47 9.394810796679954E-4 48-49 0.0022057381870465983 50-51 5.514345467616496E-4 52-53 0.0013887981177700803 54-55 0.0019402326645317298 56-57 0.003308607280569897 58-59 0.004819946408731455 60-61 0.0014909156264296451 62-63 7.148225606169532E-4 64-65 0.0011232925952552122 66-67 0.007229919613097184 68-69 0.006596791059407882 70-71 0.008169400692765179 72-73 0.005881968498790929 74-75 0.008373635710084308 76-77 0.004186817855042154 78-79 0.0039621593359911115 80-81 0.001531762629893471 82-83 0.0 84-85 3.6762303117443307E-4 86-87 0.0013275276125743415 88-89 0.004186817855042154 90-91 0.005739003986667538 92-93 0.007801777661590746 94-95 0.007229919613097184 96-97 0.009517351807071433 98-99 0.006555944055944056 100-101 0.006290438533429188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2448160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.953907843260964 #Duplication Level Percentage of deduplicated Percentage of total 1 91.52215302682124 53.955885751600476 2 4.193163235939513 4.944067179666559 3 1.1538943164426658 2.0407973757727063 4 0.7257254691659384 1.7113740971486422 5 0.4528062434698295 1.3347348774186758 6 0.26721749603048184 0.9452109381052785 7 0.2602343347890841 1.0739281693565588 8 0.16188647550619106 0.7635072286449849 9 0.13323563918736572 0.7069285434680935 >10 0.9012503831421079 10.288252295411452 >50 0.1056338336183332 4.379348576119178 >100 0.11650297879480405 13.603500480985192 >500 0.00517615576371398 2.0351322143594097 >1k 9.118268122541468E-4 0.8236867387256961 >5k 1.3905634420700847E-4 0.5658610760687536 >10k+ 6.952817210176554E-5 0.8277844571482406 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 20195 0.8249052349519639 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7180 0.2932814848702699 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6625 0.27061139794784655 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3417 0.13957421083589308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.4508202078295536E-4 0.0 0.0 2 2.4508202078295536E-4 0.0 0.0 3 2.8592902424678127E-4 0.0 0.0 4 3.67623031174433E-4 0.0 4.0847003463825896E-5 5 4.901640415659107E-4 0.0 4.0847003463825896E-5 6 6.535520554212143E-4 0.0 4.0847003463825896E-5 7 8.169400692765178E-4 0.0 4.0847003463825896E-5 8 8.986340762041696E-4 0.0 4.0847003463825896E-5 9 0.0012662571073786028 0.0 4.0847003463825896E-5 10-11 0.0020831971766551204 0.0 4.0847003463825896E-5 12-13 0.0032881837788379846 0.0 4.0847003463825896E-5 14-15 0.004472746879288936 0.0 6.127050519573884E-5 16-17 0.0056573099797398866 0.0 8.169400692765179E-5 18-19 0.006658061564603621 0.0 8.169400692765179E-5 20-21 0.008679988236063002 0.0 8.169400692765179E-5 22-23 0.010783608914450037 0.0 8.169400692765179E-5 24-25 0.013295699627475328 0.0 8.169400692765179E-5 26-27 0.01586906084569636 0.0 8.169400692765179E-5 28-29 0.018340304555257828 0.0 8.169400692765179E-5 30-31 0.026836481275733612 0.0 8.169400692765179E-5 32-33 0.03051271158747794 0.0 8.169400692765179E-5 34-35 0.03412767139402653 2.0423501731912948E-5 8.169400692765179E-5 36-37 0.03762009019018364 4.0847003463825896E-5 8.169400692765179E-5 38-39 0.04168436703483432 4.0847003463825896E-5 8.169400692765179E-5 40-41 0.045952878896804134 4.0847003463825896E-5 8.169400692765179E-5 42-43 0.05087494281419515 4.0847003463825896E-5 8.169400692765179E-5 44-45 0.05540896019867983 4.0847003463825896E-5 8.169400692765179E-5 46-47 0.06014721260048363 4.0847003463825896E-5 8.169400692765179E-5 48-49 0.06627426312005752 4.0847003463825896E-5 8.169400692765179E-5 50-51 0.07285063067773348 4.0847003463825896E-5 8.169400692765179E-5 52-53 0.0798150447683158 4.0847003463825896E-5 8.169400692765179E-5 54-55 0.08741258741258742 4.0847003463825896E-5 8.169400692765179E-5 56-57 0.09533690608456963 4.0847003463825896E-5 8.169400692765179E-5 58-59 0.10336334226521142 4.0847003463825896E-5 8.169400692765179E-5 60-61 0.11198205999607869 4.0847003463825896E-5 8.169400692765179E-5 62-63 0.12129517678583099 4.0847003463825896E-5 1.6338801385530358E-4 64-65 0.1314252336448598 4.0847003463825896E-5 1.6338801385530358E-4 66-67 0.14200460754199073 4.0847003463825896E-5 1.6338801385530358E-4 68-69 0.15382981504476834 4.0847003463825896E-5 2.0423501731912946E-4 70-71 0.167983301744984 4.0847003463825896E-5 2.246585190510424E-4 72-73 0.18356643356643357 4.0847003463825896E-5 2.4508202078295536E-4 74-75 0.2005179400039213 4.0847003463825896E-5 2.4508202078295536E-4 76-77 0.2217379583033789 4.0847003463825896E-5 2.4508202078295536E-4 78-79 0.24471439775178092 4.0847003463825896E-5 2.4508202078295536E-4 80-81 0.27008038690281677 4.0847003463825896E-5 2.4508202078295536E-4 82-83 0.3000212404418012 4.0847003463825896E-5 2.4508202078295536E-4 84-85 0.3334545127769427 4.0847003463825896E-5 2.4508202078295536E-4 86-87 0.37029850990131363 4.0847003463825896E-5 2.4508202078295536E-4 88-89 0.41637392980850924 4.0847003463825896E-5 2.8592902424678127E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTTG 15 6.620491E-4 94.993996 6 CGTCCCG 30 1.3681583E-6 79.16328 4 CGAGCGT 20 0.0020737012 71.255684 2 TCGGAAG 3905 0.0 57.5398 3 ATCGGAA 3960 0.0 56.86059 2 CGGAAGA 3945 0.0 56.708603 4 GATCGGA 3990 0.0 56.531227 1 CGCGAGG 35 2.7636476E-4 54.384468 1 GAAGAGC 4425 0.0 50.556126 6 GCCGTCT 2520 0.0 43.54158 50-51 ATGCCGT 2525 0.0 43.267242 48-49 TGCCGTC 2515 0.0 43.155983 50-51 CGTATGC 2520 0.0 42.88186 46-47 TATGCCG 2530 0.0 42.806244 48-49 TCGTATG 2575 0.0 42.518467 44-45 TCTCGTA 2540 0.0 42.075832 42-43 CGTCTTC 2600 0.0 42.021294 52-53 CCGTCTT 2590 0.0 41.908432 52-53 CTCGTAT 2520 0.0 41.844303 44-45 AATCTCG 2505 0.0 41.81044 40-41 >>END_MODULE