FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448160
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT201950.8249052349519639TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC71800.2932814848702699No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66250.27061139794784655No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA34170.13957421083589308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG156.620491E-494.9939966
CGTCCCG301.3681583E-679.163284
CGAGCGT200.002073701271.2556842
TCGGAAG39050.057.53983
ATCGGAA39600.056.860592
CGGAAGA39450.056.7086034
GATCGGA39900.056.5312271
CGCGAGG352.7636476E-454.3844681
GAAGAGC44250.050.5561266
GCCGTCT25200.043.5415850-51
ATGCCGT25250.043.26724248-49
TGCCGTC25150.043.15598350-51
CGTATGC25200.042.8818646-47
TATGCCG25300.042.80624448-49
TCGTATG25750.042.51846744-45
TCTCGTA25400.042.07583242-43
CGTCTTC26000.042.02129452-53
CCGTCTT25900.041.90843252-53
CTCGTAT25200.041.84430344-45
AATCTCG25050.041.8104440-41
Produced by FastQC (version 0.11.2)