##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2801657 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.666519848789484 33.0 33.0 33.0 33.0 33.0 2 32.675370325489524 33.0 33.0 33.0 33.0 33.0 3 32.72410898264848 33.0 33.0 33.0 33.0 33.0 4 36.64039352426082 37.0 37.0 37.0 37.0 37.0 5 36.65400404117992 37.0 37.0 37.0 37.0 37.0 6 36.54998024383428 37.0 37.0 37.0 37.0 37.0 7 36.364325111889144 37.0 37.0 37.0 37.0 37.0 8 36.31524308650202 37.0 37.0 37.0 37.0 37.0 9 36.40500353897711 37.0 37.0 37.0 37.0 37.0 10-11 36.293025698720434 37.0 37.0 37.0 35.0 37.0 12-13 36.30551330873122 37.0 37.0 37.0 35.0 37.0 14-15 38.11575310610827 40.0 37.0 40.0 35.0 40.0 16-17 38.104344678881105 40.0 37.0 40.0 37.0 40.0 18-19 38.060899674728205 40.0 37.0 40.0 37.0 40.0 20-21 38.101397494411344 40.0 37.0 40.0 37.0 40.0 22-23 37.78030072917563 40.0 37.0 40.0 33.0 40.0 24-25 37.961562925083264 40.0 37.0 40.0 33.0 40.0 26-27 37.873467023265164 40.0 37.0 40.0 33.0 40.0 28-29 37.60821024843513 40.0 37.0 40.0 33.0 40.0 30-31 37.55026578913835 40.0 37.0 40.0 33.0 40.0 32-33 37.485230169146334 40.0 37.0 40.0 33.0 40.0 34-35 37.325851629946136 40.0 37.0 40.0 33.0 40.0 36-37 37.29076792769422 40.0 37.0 40.0 33.0 40.0 38-39 37.24317305794392 40.0 37.0 40.0 33.0 40.0 40-41 37.19753328119752 40.0 37.0 40.0 33.0 40.0 42-43 36.497979410041985 37.0 37.0 40.0 33.0 40.0 44-45 36.28539860518258 37.0 37.0 40.0 33.0 40.0 46-47 36.726746707394945 37.0 37.0 40.0 33.0 40.0 48-49 36.401910726402264 37.0 37.0 40.0 33.0 40.0 50-51 36.39286001105774 37.0 37.0 40.0 33.0 40.0 52-53 36.36383522322683 37.0 37.0 40.0 33.0 40.0 54-55 35.97144671885245 37.0 37.0 40.0 30.0 40.0 56-57 35.85810647056367 37.0 37.0 40.0 33.0 40.0 58-59 35.741963416649504 37.0 35.0 40.0 27.0 40.0 60-61 35.443590168246864 37.0 33.0 40.0 27.0 40.0 62-63 35.01117731399668 37.0 33.0 37.0 27.0 40.0 64-65 34.70208469487878 37.0 33.0 37.0 27.0 40.0 66-67 33.83391328774364 37.0 33.0 37.0 27.0 40.0 68-69 33.648441261724756 37.0 33.0 37.0 27.0 37.0 70-71 33.066657517319214 37.0 33.0 37.0 24.5 37.0 72-73 31.535961218664525 33.0 30.0 37.0 18.5 37.0 74-75 30.456814663608 33.0 27.0 37.0 15.0 37.0 76-77 27.850881638972936 30.0 24.5 33.0 15.0 35.0 78-79 29.81054675857894 33.0 27.0 37.0 15.0 37.0 80-81 30.1356611462431 33.0 27.0 37.0 15.0 37.0 82-83 29.959707059072542 33.0 27.0 37.0 6.0 37.0 84-85 29.746869263439457 33.0 27.0 37.0 6.0 37.0 86-87 29.54654709695013 33.0 27.0 37.0 6.0 37.0 88-89 29.419556355399678 33.0 27.0 37.0 6.0 37.0 90-91 29.27488000850925 33.0 27.0 37.0 6.0 37.0 92-93 29.092359093208053 33.0 27.0 37.0 6.0 37.0 94-95 28.86095996048053 33.0 27.0 37.0 6.0 37.0 96-97 28.68910844546638 33.0 27.0 37.0 6.0 37.0 98-99 28.541590030471255 33.0 27.0 37.0 6.0 37.0 100-101 27.059405380458777 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13614050141711687 2306 2 0.13376729854847014 2306 3 0.1286146400603272 2306 4 0.1504710507640752 2306 5 0.17133063311691643 2306 6 0.2083970823975676 2306 7 0.22107902527991996 2306 8 0.19861938089860587 2306 9 0.18841902768361507 2306 10-11 0.2712331419303595 2306 12-13 0.2753659755390174 2306 14-15 0.4436484797188527 2306 16-17 0.34452800299632 2306 18-19 0.4334132496122223 2306 20-21 0.43144171102314743 2306 22-23 0.4169987970565785 2306 24-25 0.4315163218088358 2306 26-27 0.43489769140344237 2306 28-29 0.5086016323454672 2306 30-31 0.5616253116448888 2306 32-33 0.5824855382307419 2306 34-35 0.6098288978505266 2306 36-37 0.5182600541204252 2306 38-39 0.5272511582172115 2306 40-41 0.5499188958964467 2306 42-43 0.6616870315108372 2306 44-45 0.5843282016307256 2306 46-47 0.419156090501815 2306 48-49 0.47018813375009216 2306 50-51 0.46437147053904937 2306 52-53 0.618547208478347 2306 54-55 0.4733122688262057 2306 56-57 0.4676184648055397 2306 58-59 0.5839233671062871 2306 60-61 0.6123088629679785 2306 62-63 0.44841040774245755 2306 64-65 0.4491101325398503 2306 66-67 0.3793764669392843 2306 68-69 0.5748761505119191 2306 70-71 0.7089237044405223 2306 72-73 0.10285563863381242 2306 74-75 0.11699423781726281 2306 76-77 -0.04703919811242585 2306 78-79 0.23229376727000783 2306 80-81 0.35917883530224515 2306 82-83 0.4277903789193793 2306 84-85 0.40406770069857956 2306 86-87 0.3343964775482142 2306 88-89 0.42556792317121506 2306 90-91 0.35687104137298675 2306 92-93 0.44616048285907084 2306 94-95 0.4162367458733236 2306 96-97 0.4249948806870769 2306 98-99 0.4820933975409609 2306 100-101 0.2673164080041843 2307 1 0.10405924194055416 2307 2 0.11452719537753353 2307 3 0.10486595853880232 2307 4 0.1782761137214237 2307 5 0.13104349322635045 2307 6 0.20131499887195758 2307 7 0.165066969442357 2307 8 0.26738009353621806 2307 9 0.2253242702836502 2307 10-11 0.2653334657816302 2307 12-13 0.19565797731895884 2307 14-15 0.3852083633631267 2307 16-17 0.4419970393533603 2307 18-19 0.3590635273167564 2307 20-21 0.34968162466280717 2307 22-23 0.3989483116916901 2307 24-25 0.4831452607632798 2307 26-27 0.43629661766753003 2307 28-29 0.4351824674367748 2307 30-31 0.4961140304134375 2307 32-33 0.42593918959207144 2307 34-35 0.4986527326451693 2307 36-37 0.5139559429397238 2307 38-39 0.5712084065526284 2307 40-41 0.5197357303944372 2307 42-43 0.38819349617561727 2307 44-45 0.4683683286990856 2307 46-47 0.4622453061719298 2307 48-49 0.4666878824151297 2307 50-51 0.5493416399635294 2307 52-53 0.47893620083116417 2307 54-55 0.2970355078333995 2307 56-57 0.2887184466764623 2307 58-59 0.42790162869241755 2307 60-61 0.3248813792841574 2307 62-63 0.242624656374538 2307 64-65 0.3335814432722657 2307 66-67 0.25178040342158425 2307 68-69 0.2394529601236215 2307 70-71 0.13284667233557457 2307 72-73 -0.47738109005628004 2307 74-75 -0.5450351271705394 2307 76-77 -0.405156184235441 2307 78-79 -0.25611721895189277 2307 80-81 0.030455060663179268 2307 82-83 0.06775675771824652 2307 84-85 0.09833551251874084 2307 86-87 -0.053072723618640794 2307 88-89 -0.015200049338506716 2307 90-91 -0.1359926938765348 2307 92-93 -0.07108830578882674 2307 94-95 -0.03555030749209109 2307 96-97 0.03921343497543006 2307 98-99 -0.03141616554823301 2307 100-101 -0.25279832022443927 2201 1 0.1593004740375008 2201 2 0.1489840597428156 2201 3 0.12322729407559052 2201 4 0.10020352221695816 2201 5 0.09030908571012475 2201 6 0.09439286948035175 2201 7 0.1440756455348975 2201 8 0.04516424704338107 2201 9 0.15482107221779273 2201 10-11 0.195089823658968 2201 12-13 0.1278670145328178 2201 14-15 0.26335784616396296 2201 16-17 0.21794020100105627 2201 18-19 0.20876954243487944 2201 20-21 0.16884171157675354 2201 22-23 0.2398860412179502 2201 24-25 0.18050303099219178 2201 26-27 0.2643621321123888 2201 28-29 0.3505647434191488 2201 30-31 0.313976146339499 2201 32-33 0.3142626150419474 2201 34-35 0.18067394087139377 2201 36-37 0.28955290248190124 2201 38-39 0.24576618596259436 2201 40-41 0.14141964978481525 2201 42-43 0.333879741432078 2201 44-45 0.308778147832399 2201 46-47 0.18201727183480187 2201 48-49 0.22252186607286006 2201 50-51 0.3381881940093123 2201 52-53 0.2553328681099103 2201 54-55 0.1768102278113588 2201 56-57 0.2202718038805287 2201 58-59 0.2737755116646312 2201 60-61 0.13177175754342585 2201 62-63 0.11092268091854152 2201 64-65 0.14180670799255068 2201 66-67 0.3769722491187295 2201 68-69 0.07975279792506029 2201 70-71 0.07436491202219742 2201 72-73 -0.21531738121798583 2201 74-75 -0.22687979145013415 2201 76-77 -0.12080815357073149 2201 78-79 0.11763798364328437 2201 80-81 0.09424480696015536 2201 82-83 0.3503005207247334 2201 84-85 0.23910721805185275 2201 86-87 0.16131493857991686 2201 88-89 0.29339150103485423 2201 90-91 0.21971179871741597 2201 92-93 0.008605888727025501 2201 94-95 -0.07539970001259633 2201 96-97 0.24589953486525573 2201 98-99 0.08310079644221702 2201 100-101 -0.00650956854124729 1101 1 0.04950083633877256 1101 2 0.0873756220738855 1101 3 0.10170396978924146 1101 4 0.08936541408471754 1101 5 0.09284217188567112 1101 6 0.04756961331001719 1101 7 0.0902028390075742 1101 8 0.05272449175490124 1101 9 0.10983990077703254 1101 10-11 0.02858624176897706 1101 12-13 0.0425879111054428 1101 14-15 0.05762024956961653 1101 16-17 0.026004532321834972 1101 18-19 -0.024110070773019743 1101 20-21 0.07092195631638987 1101 22-23 0.1142025086329781 1101 24-25 0.1295302513879122 1101 26-27 0.1577572338048796 1101 28-29 0.18665927072986932 1101 30-31 0.09179000896727985 1101 32-33 0.17581998327541726 1101 34-35 0.02530880319479678 1101 36-37 -0.02523656702160082 1101 38-39 0.023670905087726624 1101 40-41 0.014141822917586921 1101 42-43 0.13906566159223388 1101 44-45 0.062028420401887274 1101 46-47 0.007589667768087338 1101 48-49 0.05981475016386639 1101 50-51 0.13213637803923461 1101 52-53 -0.013171278953201693 1101 54-55 0.10082502353712641 1101 56-57 0.01105196068090919 1101 58-59 0.05006484795878663 1101 60-61 1.4652636021139642E-4 1101 62-63 0.005603129654737415 1101 64-65 0.0036797015050069604 1101 66-67 0.09439711940658668 1101 68-69 0.011722018485279762 1101 70-71 -0.05006525781794835 1101 72-73 -0.29635100831480443 1101 74-75 -0.2983349997241582 1101 76-77 -0.41656977705156706 1101 78-79 -0.15619559115393145 1101 80-81 0.01681152234136718 1101 82-83 0.09370894335049584 1101 84-85 0.2750591516257366 1101 86-87 0.11921446289255044 1101 88-89 -0.03215224961976659 1101 90-91 -0.08466256379333359 1101 92-93 0.013809663858424415 1101 94-95 -0.08808586796320483 1101 96-97 -0.007898512756902676 1101 98-99 -0.02090898020896148 1101 100-101 -0.14213072822866124 2304 1 0.08924233871103837 2304 2 0.09932671554762607 2304 3 0.10360963742628826 2304 4 0.10639069825193559 2304 5 0.1296474611610705 2304 6 0.11487845422191612 2304 7 0.1582010201261852 2304 8 0.0653451262910778 2304 9 0.08957514661970833 2304 10-11 -0.009201250055632215 2304 12-13 0.06902059368489688 2304 14-15 0.07410798696960796 2304 16-17 0.15237785046702612 2304 18-19 0.1326834776098167 2304 20-21 0.12023601496277081 2304 22-23 0.09472196558204615 2304 24-25 0.05332433500762335 2304 26-27 0.13789539223368052 2304 28-29 0.20700916120760837 2304 30-31 0.13221656173167418 2304 32-33 0.12010024148266041 2304 34-35 0.13151174310373648 2304 36-37 0.11993603861838409 2304 38-39 0.22152589879490137 2304 40-41 0.2925037671143613 2304 42-43 0.28654767241972934 2304 44-45 0.23004676364909216 2304 46-47 0.07619898247350676 2304 48-49 0.14204290626296512 2304 50-51 0.1549085288268799 2304 52-53 0.15437831955315318 2304 54-55 0.21860468810002942 2304 56-57 0.12710447989103102 2304 58-59 0.15265955881539384 2304 60-61 0.11756469154710913 2304 62-63 0.17117974343349118 2304 64-65 0.0752704373534101 2304 66-67 0.17597140876579687 2304 68-69 0.12682186886974733 2304 70-71 0.0933526486601366 2304 72-73 0.03594693976788221 2304 74-75 0.3993827199160016 2304 76-77 -0.0021829268512334465 2304 78-79 0.37498624616706167 2304 80-81 0.5722798150017603 2304 82-83 0.7039939718315651 2304 84-85 0.5660570746955038 2304 86-87 0.44308738010506943 2304 88-89 0.567609038697352 2304 90-91 0.4475034559912956 2304 92-93 0.4598574906713466 2304 94-95 0.4838815466051365 2304 96-97 0.4001654807172983 2304 98-99 0.35553594484137463 2304 100-101 0.3586818616512062 2202 1 0.023652798136829745 2202 2 0.07364719048143797 2202 3 0.00319108638125698 2202 4 0.05367324134627438 2202 5 0.07648026561237486 2202 6 0.06957708950409369 2202 7 0.014455297698255265 2202 8 0.02951492652501031 2202 9 0.11882776555076191 2202 10-11 0.08955774088771307 2202 12-13 0.09031409471641894 2202 14-15 0.02859829644984302 2202 16-17 0.08705147439851402 2202 18-19 -0.005083294302224317 2202 20-21 0.11138124929559723 2202 22-23 0.13515815692071698 2202 24-25 -0.026887991875163664 2202 26-27 0.09312479876860635 2202 28-29 0.10296803891974804 2202 30-31 0.05286894010770027 2202 32-33 -0.046489171032447985 2202 34-35 -0.0536463914980132 2202 36-37 -0.08260336099890964 2202 38-39 0.0991746235039983 2202 40-41 -0.05799367201008465 2202 42-43 -0.11082031488955124 2202 44-45 -0.05776406304283199 2202 46-47 -0.11469868115262472 2202 48-49 -0.2670225748256456 2202 50-51 -0.047311335773599694 2202 52-53 -0.04487887641831634 2202 54-55 -0.015825409096208887 2202 56-57 0.02178782160846282 2202 58-59 0.045332403363289586 2202 60-61 -0.05978704634961929 2202 62-63 -0.29284499464276337 2202 64-65 -0.15518661107437737 2202 66-67 -0.1439945054566607 2202 68-69 -0.15894322908321357 2202 70-71 -0.07809473773949804 2202 72-73 -0.42076847309403576 2202 74-75 -0.39514783933461217 2202 76-77 -0.7952893647925059 2202 78-79 -0.46316283422770255 2202 80-81 -0.24467533184035872 2202 82-83 -0.16141852244713562 2202 84-85 -0.2655355638059227 2202 86-87 -0.2827765439319876 2202 88-89 -0.15279491115985522 2202 90-91 -0.1923249754563443 2202 92-93 -0.38316227760908816 2202 94-95 -0.24979199093241178 2202 96-97 -0.3926404166185229 2202 98-99 -0.3525579414703337 2202 100-101 -0.2853636354784932 1102 1 0.05580353501860458 1102 2 0.08775528816103417 1102 3 0.07365277739005904 1102 4 0.0390640371382176 1102 5 0.05440045198923116 1102 6 -0.0017087492871041832 1102 7 0.05560912702704002 1102 8 0.0671315662902714 1102 9 0.01522209797143148 1102 10-11 0.04028974339576763 1102 12-13 0.013676202650330538 1102 14-15 0.06075604377549837 1102 16-17 0.036909134345997074 1102 18-19 0.023020173119398635 1102 20-21 -0.12302640184676505 1102 22-23 -0.07880619861759186 1102 24-25 0.12608984839214799 1102 26-27 0.02795024432437998 1102 28-29 -0.026721527408589907 1102 30-31 0.04465508343258051 1102 32-33 0.019106991506653515 1102 34-35 0.07872452940511465 1102 36-37 0.05743670508994825 1102 38-39 0.020527257804474175 1102 40-41 -0.026303941917781515 1102 42-43 -0.08048976965648791 1102 44-45 -0.0481380225527559 1102 46-47 -0.055489874240436166 1102 48-49 0.020468718203055403 1102 50-51 -0.07865909641349589 1102 52-53 -0.002778637165882003 1102 54-55 -0.10020998185733276 1102 56-57 -0.26869594949437925 1102 58-59 -0.18641169973174243 1102 60-61 0.012270922730401423 1102 62-63 -0.07449841063577622 1102 64-65 -0.05683414951123922 1102 66-67 -0.042280255635851915 1102 68-69 0.056928107399023986 1102 70-71 -0.20494787847443519 1102 72-73 -0.29494138092520217 1102 74-75 -0.3852942398362238 1102 76-77 -0.5629088536021563 1102 78-79 -0.20564777859843275 1102 80-81 -0.08305515656804374 1102 82-83 -0.038318352738311745 1102 84-85 0.009802283505486287 1102 86-87 0.06153285443732415 1102 88-89 0.06273165169242034 1102 90-91 0.251341621292827 1102 92-93 0.08160407270896286 1102 94-95 0.12325630149171118 1102 96-97 0.061645320100609524 1102 98-99 -0.03209961547459628 1102 100-101 -0.1327067333319505 2305 1 -0.01663490272363788 2305 2 0.05681994961907577 2305 3 0.030294504869495142 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-0.1252528509509716 1213 96-97 -0.24036135515903467 1213 98-99 -0.22500818601476524 1213 100-101 0.06715759408355737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 7.0 10 17.0 11 20.0 12 26.0 13 70.0 14 282.0 15 719.0 16 1557.0 17 2501.0 18 3482.0 19 4492.0 20 6212.0 21 8480.0 22 11525.0 23 15818.0 24 21441.0 25 29039.0 26 40606.0 27 60842.0 28 108914.0 29 185734.0 30 141665.0 31 73136.0 32 84487.0 33 120662.0 34 186645.0 35 322755.0 36 566403.0 37 691566.0 38 112518.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.26896658472249 19.710534622276924 19.7600085506963 12.260490242304284 2 28.982776978052634 31.445569532601596 28.548141332075982 11.023512157269787 3 36.96922212009531 14.152756088201961 35.128903008183045 13.74911878351969 4 29.18065334131008 13.682216088947396 28.26631390024402 28.870816669498506 5 44.10083033005111 12.225836353272367 29.177340409621877 14.495992907054648 6 38.73057979617062 13.070729214889617 33.46323265124889 14.735458337690874 7 31.9018352353625 28.717826629027037 23.094904194196506 16.285433941413956 8 27.647388670347585 37.93851281580864 18.675912147704018 15.738186366139754 9 42.9028963930988 21.24446354425256 18.95057103706842 16.902069025580218 10-11 31.277954439105145 30.8263823872801 22.929966087925823 14.965697085688934 12-13 23.98553784421148 29.219065717180943 22.466347593584796 24.329048845022786 14-15 23.168449956579266 29.585152643596274 22.689037237606176 24.557360162218288 16-17 29.562724766580057 23.0135100430763 23.645421913785647 23.778343276557994 18-19 20.924170945979466 23.118497374946326 31.30531324855255 24.652018430521654 20-21 27.50338460418245 24.032670665966606 31.305331095134058 17.158613634716886 22-23 20.174579141163857 38.2023891618231 24.034516064093616 17.588515632919435 24-25 20.584829219723837 22.089100533595225 32.3496232852326 24.976446961448328 26-27 19.131053515830097 22.228559741610056 25.342359896304224 33.298026846255624 28-29 26.079298786396766 30.759939564336392 23.699689148243344 19.4610725010235 30-31 17.289732469035286 24.528573626250466 31.29367727741119 26.888016627303056 32-33 17.78094177838329 30.736489156238612 24.017518918268724 27.46505014710937 34-35 18.855181058923346 30.36399530706293 31.673523918167 19.10729971584673 36-37 17.671328074778607 32.38540620782629 32.12234759644025 17.820918120954847 38-39 17.640301210044285 25.03409618604578 30.21296865078331 27.112633953126625 40-41 26.344891612356548 23.963497316052607 22.60385550408205 27.087755567508797 42-43 19.464195652786902 31.41792517785011 30.39437018878471 18.72350898057828 44-45 17.9247911186638 25.1316857687995 30.133487385382296 26.810035727154403 46-47 26.16304190115074 24.290613730330865 23.715269988412103 25.83107438010629 48-49 18.703029911476847 30.953405458020438 31.577713104035627 18.765851526467088 50-51 25.339863315820978 23.849112002572777 29.789148573996453 21.021876107609792 52-53 17.396483032304904 24.505589270200293 23.22049258678378 34.877435110711026 54-55 25.709298745800375 24.425080423152945 31.335430358680572 18.530190472366115 56-57 18.88047525340554 24.288845730040332 30.083307841374218 26.747371175179914 58-59 17.961841006693227 23.973965273467073 30.36654793218892 27.69764578765078 60-61 24.509380495721924 23.59637008849551 31.866611745884775 20.027637669897782 62-63 17.661261667073276 23.35427765470478 31.77239929048561 27.212061387736338 64-65 26.171343987758515 25.01151565263188 30.283034307096802 18.534106052512804 66-67 19.3133529031391 38.69943666159841 23.038011554478803 18.949198880783687 68-69 18.44586359225493 37.226072316569315 24.706397213011208 19.621666878164547 70-71 17.99827657353691 39.43767678685912 23.61203128725821 18.952015352345757 72-73 19.821939846559072 38.833858236518054 23.139949076716537 18.204252840206333 74-75 21.139624876244714 36.631296794758185 23.35280755609124 18.87627077290586 76-77 20.555600075173704 34.682063593171044 23.8929592114828 20.86937712017245 78-79 20.31038424573737 34.70324671094608 24.415662526960737 20.57070651635581 80-81 19.559888149868325 34.919917361684696 24.56965754642159 20.950536942025398 82-83 19.182594443216995 34.17104592032501 24.727045459169343 21.919314177288655 84-85 19.97160955680712 33.16372476618218 24.67263006367326 22.192035613337442 86-87 18.86744771505333 33.41833775399154 25.437363181788815 22.27685134916632 88-89 18.503322394622774 33.60764885186373 25.586748757861137 22.30227999565236 90-91 19.297481602476417 32.602578508951034 24.833747640519384 23.266192248053162 92-93 19.843025196285435 32.407345599797246 24.112222259901728 23.637406944015595 94-95 20.176100229312606 33.482745868517924 23.57800483755358 22.763149064615895 96-97 19.704464446669558 34.21694144440815 22.839893551513462 23.238700557408826 98-99 19.685394221012924 32.41779904459934 24.54451590564504 23.352290828742692 100-101 20.64177397465159 33.42467332848344 23.721745760570105 22.21180693629486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1262.0 1 750.5 2 354.5 3 575.5 4 521.0 5 327.5 6 316.5 7 348.5 8 379.5 9 386.0 10 339.0 11 317.5 12 311.0 13 283.5 14 277.5 15 289.5 16 304.5 17 292.0 18 289.0 19 327.5 20 359.0 21 389.0 22 420.0 23 446.5 24 512.5 25 622.5 26 813.5 27 1095.0 28 1386.5 29 1841.0 30 2447.5 31 3516.0 32 5893.0 33 10579.5 34 18894.0 35 33282.0 36 54253.5 37 83028.0 38 120290.0 39 151658.0 40 169148.0 41 170672.0 42 184555.5 43 213183.5 44 203371.0 45 174579.5 46 168440.0 47 162991.0 48 141324.0 49 117042.5 50 95741.5 51 82857.5 52 78791.5 53 80130.0 54 71805.0 55 46629.0 56 28170.0 57 22778.0 58 21559.5 59 19057.5 60 14863.5 61 11120.5 62 7500.5 63 4747.5 64 3192.0 65 2193.5 66 1541.5 67 1166.5 68 906.5 69 693.0 70 482.5 71 321.0 72 232.0 73 170.5 74 112.5 75 65.0 76 45.5 77 37.5 78 24.0 79 17.0 80 12.5 81 9.0 82 7.5 83 4.5 84 5.5 85 5.5 86 2.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1507679205555855 2 0.0 3 0.004711497517362047 4 0.006460462504867655 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.784658150515927E-5 18-19 0.0 20-21 0.0 22-23 1.7846581505159267E-4 24-25 5.35397445154778E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.566359122476448E-4 40-41 0.0 42-43 0.0 44-45 2.320055595670705E-4 46-47 9.815619827837597E-4 48-49 0.0026234474812584126 50-51 5.710906081650966E-4 52-53 0.001392033357402423 54-55 0.002480674829217138 56-57 0.004283179561238224 58-59 0.005996451385733514 60-61 0.0016418854984746527 62-63 8.030961677321671E-4 64-65 0.001267107286866308 66-67 0.00829866039989906 68-69 0.0075134108136720515 70-71 0.009315915545693138 72-73 0.006710314645939884 74-75 0.009333762127198298 76-77 0.005193355218001347 78-79 0.004622264609836251 80-81 0.0018203513135262453 82-83 0.0 84-85 3.5693163010318534E-4 86-87 0.0013563401943921043 88-89 0.004943503076929117 90-91 0.006888780460991478 92-93 0.008994677078600272 94-95 0.008602052285486767 96-97 0.0107793352291162 98-99 0.007656183465713326 100-101 0.007816802699259759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2801657.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.901165871624347 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45148358707587 19.037310739335425 2 9.33734135326631 4.650213716753235 3 4.101623737758928 3.0640563911078074 4 2.138175555777208 2.1297225670824336 5 1.4732230302762068 1.8342485521402445 6 1.0060778785949727 1.5031507280799212 7 0.6666531672850151 1.1620308768185528 8 0.6148672162922321 1.2248728433533438 9 0.4859920036593018 1.089159074586301 >10 2.9958679184703447 14.958872145292162 >50 0.3608189456043439 6.367814842456741 >100 0.32646183284062646 16.350959007944518 >500 0.028775271298574503 4.748465081340935 >1k 0.011334564036965946 5.187058388553046 >5k 5.795278946675825E-4 1.030692400275397 >10k+ 7.244098682844277E-4 15.661372644880037 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 373899 13.34563795639509 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20005 0.7140417260214224 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 18638 0.6652491721863169 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11130 0.3972649043048453 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10434 0.3724224628496636 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8382 0.29918009235248993 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7551 0.2695190738909153 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7467 0.2665208481980485 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5169 0.18449795960033652 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4877 0.17407555600132352 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4867 0.17371862437122032 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 3980 0.14205878878106776 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3814 0.1361337237213549 TruSeq Adapter, Index 27 (97% over 38bp) TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3705 0.13224316895323018 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3626 0.129423409075415 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3568 0.12735320562081653 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3520 0.12563993379632124 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3502 0.12499745686213551 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3326 0.11871546017231945 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006603235156908929 0.0 0.0 2 0.007210018928084344 0.0 0.0 3 0.008102348003342308 0.0 0.0 4 0.008602052285486767 0.0 0.0 5 0.010136858294930464 0.0 0.0 6 0.011564584815343205 0.0 0.0 7 0.013563401943921045 0.0 0.0 8 0.015205287442395696 0.0 0.0 9 0.017025638755921942 0.0 0.0 10-11 0.025288605992810683 0.0 0.0 12-13 0.03249862492089503 0.0 0.0 14-15 0.04163607465153657 0.0 0.0 16-17 0.05127322866432258 0.0 0.0 18-19 0.06144578012226336 0.0 0.0 20-21 0.06863795246884255 0.0 0.0 22-23 0.07420608589845223 0.0 0.0 24-25 0.08066654840331988 0.0 0.0 26-27 0.08735901646775462 0.0 0.0 28-29 0.09506873967798343 0.0 0.0 30-31 0.17885843984470617 0.0 0.0 32-33 0.19017317251897714 0.0 0.0 34-35 0.19879307138596908 0.0 0.0 36-37 0.2094653271260543 0.0 0.0 38-39 0.2214760764790265 0.0 0.0 40-41 0.23561056903111266 0.0 0.0 42-43 0.25231496931994174 0.0 0.0 44-45 0.2702329371511216 0.0 0.0 46-47 0.2886149161014357 0.0 0.0 48-49 0.30887078610979146 0.0 0.0 50-51 0.3326959724191791 0.0 0.0 52-53 0.357788266015433 0.0 0.0 54-55 0.38587878530455366 0.0 0.0 56-57 0.4158075024887058 0.0 0.0 58-59 0.44880583169174526 0.0 0.0 60-61 0.48148292242769186 0.0 0.0 62-63 0.517015466204464 0.0 0.0 64-65 0.5579555241772993 0.0 0.0 66-67 0.6013227172348363 0.0 0.0 68-69 0.6496334133693025 0.0 0.0 70-71 0.7010315681041612 0.0 0.0 72-73 0.7584440208062586 0.0 0.0 74-75 0.821246141122914 0.0 0.0 76-77 0.8935069496373039 0.0 0.0 78-79 0.9742091912036341 0.0 0.0 80-81 1.0630137807733067 0.0 0.0 82-83 1.1684335377242823 0.0 0.0 84-85 1.2913429445503144 0.0 0.0 86-87 1.4265843391964113 0.0 0.0 88-89 1.5708382575026136 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 48890 0.0 83.38836 3 CGGAAGA 48935 0.0 83.1646 4 GATCGGA 49140 0.0 83.06558 1 GAAGAGC 49105 0.0 82.83503 6 ATCGGAA 50010 0.0 81.55882 2 GGAAGAG 50975 0.0 79.926704 5 GAGCACA 56505 0.0 71.77666 9 AGAGCAC 56600 0.0 71.77367 8 AAGAGCA 56875 0.0 71.40157 7 CGACGTA 20 0.002074967 71.244995 5 GGTACGC 55 1.1696102E-8 60.548634 1 CATCGGA 25 0.0049912105 57.08871 1 CGTGGCA 135 0.0 52.859917 1 TTGTGTT 23590 0.0 51.526653 1 CGAGGTG 295 0.0 49.99294 1 GAACGTT 40 5.3913816E-4 47.496666 7 GTACGGG 195 0.0 46.354084 1 GCGCACA 165 0.0 46.05737 9 GCCGTCT 44520 0.0 45.87831 50-51 ATGCCGT 44440 0.0 45.82607 48-49 >>END_MODULE