##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3314319 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67357155421672 33.0 33.0 33.0 33.0 33.0 2 32.72024449064801 33.0 33.0 33.0 33.0 33.0 3 32.75054302256361 33.0 33.0 33.0 33.0 33.0 4 36.69064112416457 37.0 37.0 37.0 37.0 37.0 5 36.67607825317961 37.0 37.0 37.0 37.0 37.0 6 36.552616691392714 37.0 37.0 37.0 37.0 37.0 7 36.50575578271132 37.0 37.0 37.0 37.0 37.0 8 36.437170049111145 37.0 37.0 37.0 37.0 37.0 9 36.43131696134259 37.0 37.0 37.0 37.0 37.0 10-11 36.37433693015066 37.0 37.0 37.0 37.0 37.0 12-13 36.37883031174729 37.0 37.0 37.0 37.0 37.0 14-15 38.26953712059702 40.0 37.0 40.0 37.0 40.0 16-17 38.10750232551544 40.0 37.0 40.0 37.0 40.0 18-19 37.90872604598411 40.0 37.0 40.0 35.0 40.0 20-21 37.80448004552368 40.0 37.0 40.0 33.0 40.0 22-23 37.6549096511229 40.0 37.0 40.0 33.0 40.0 24-25 37.57302450367632 40.0 37.0 40.0 33.0 40.0 26-27 37.43772431078602 40.0 37.0 40.0 33.0 40.0 28-29 37.27500838030377 40.0 37.0 40.0 33.0 40.0 30-31 37.20945177576449 40.0 37.0 40.0 33.0 40.0 32-33 37.03996658137011 37.0 37.0 40.0 33.0 40.0 34-35 36.935843532261075 37.0 37.0 40.0 33.0 40.0 36-37 36.81989950273344 37.0 37.0 40.0 33.0 40.0 38-39 36.68978197331035 37.0 37.0 40.0 33.0 40.0 40-41 36.59286281736912 37.0 37.0 40.0 33.0 40.0 42-43 36.44561658066107 37.0 37.0 40.0 33.0 40.0 44-45 36.278381018845806 37.0 37.0 40.0 33.0 40.0 46-47 36.164402702334925 37.0 37.0 40.0 33.0 40.0 48-49 35.950573858460814 37.0 37.0 40.0 30.0 40.0 50-51 35.777219694302204 37.0 37.0 40.0 27.0 40.0 52-53 35.57989665448619 37.0 37.0 40.0 27.0 40.0 54-55 35.38127380013813 37.0 37.0 40.0 27.0 40.0 56-57 35.156100846056155 37.0 33.0 40.0 27.0 40.0 58-59 34.91158062938419 37.0 33.0 40.0 27.0 40.0 60-61 34.63997641747822 37.0 33.0 40.0 27.0 40.0 62-63 34.325355374663694 37.0 33.0 37.0 27.0 40.0 64-65 34.028300987321984 37.0 33.0 37.0 27.0 40.0 66-67 33.764286268159466 37.0 33.0 37.0 24.5 40.0 68-69 33.47327610890804 37.0 33.0 37.0 22.0 40.0 70-71 33.10328969540953 37.0 33.0 37.0 22.0 40.0 72-73 32.82980289465196 37.0 33.0 37.0 22.0 37.0 74-75 32.51002951134154 37.0 33.0 37.0 22.0 37.0 76-77 29.63374617832502 33.0 27.0 35.0 18.5 37.0 78-79 32.15864812650804 35.0 33.0 37.0 22.0 37.0 80-81 32.53490219257712 37.0 33.0 37.0 22.0 37.0 82-83 32.44758666863389 37.0 33.0 37.0 22.0 37.0 84-85 32.22488134063136 37.0 33.0 37.0 22.0 37.0 86-87 32.01877037183204 35.0 33.0 37.0 22.0 37.0 88-89 31.82629704624087 33.0 33.0 37.0 18.5 37.0 90-91 31.612904792809623 33.0 33.0 37.0 15.0 37.0 92-93 31.458425999428542 33.0 33.0 37.0 15.0 37.0 94-95 31.231111881505672 33.0 33.0 37.0 15.0 37.0 96-97 31.027442741631084 33.0 33.0 37.0 15.0 37.0 98-99 30.842653649211194 33.0 33.0 37.0 15.0 37.0 100-101 29.30376662596449 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13845148914180072 2306 2 0.11360391692918625 2306 3 0.1433675209913119 2306 4 0.14523136996456287 2306 5 0.14937151731146514 2306 6 0.18824221958384868 2306 7 0.17448026528734317 2306 8 0.17970455354550552 2306 9 0.1848388190391006 2306 10-11 0.1725053210147962 2306 12-13 0.22807216023323917 2306 14-15 0.344837462784092 2306 16-17 0.3165314914440813 2306 18-19 0.5242543221812426 2306 20-21 0.6166088121920978 2306 22-23 0.6236535681218314 2306 24-25 0.5883487500904181 2306 26-27 0.4842429286251928 2306 28-29 0.5160799190361871 2306 30-31 0.5516605815944899 2306 32-33 0.5312897719560965 2306 34-35 0.6912426556512656 2306 36-37 0.6989027894312798 2306 38-39 0.7290230538379987 2306 40-41 0.7936697034674367 2306 42-43 0.859867732869013 2306 44-45 0.8746704239975642 2306 46-47 0.898994861961576 2306 48-49 0.930745007277757 2306 50-51 0.9479724483428527 2306 52-53 0.8235819548604155 2306 54-55 0.7652211361197132 2306 56-57 0.9678993060408203 2306 58-59 0.9321822615449449 2306 60-61 0.9584491258357133 2306 62-63 0.9298826264950293 2306 64-65 0.8654771707220661 2306 66-67 0.7846999157184769 2306 68-69 0.7594952998154412 2306 70-71 0.9468741472168816 2306 72-73 0.8822691215389611 2306 74-75 0.918445368088868 2306 76-77 0.6516521687637997 2306 78-79 0.806492849791205 2306 80-81 1.1648625475289904 2306 82-83 1.2073760616076399 2306 84-85 1.2182535883131251 2306 86-87 1.2213667423704493 2306 88-89 1.2322235856794563 2306 90-91 1.278444026831668 2306 92-93 1.2177614039484652 2306 94-95 1.219701071471409 2306 96-97 1.325174446409907 2306 98-99 1.3396793825068052 2306 100-101 1.1454962749335493 2307 1 0.11028766201169304 2307 2 0.10652467426047707 2307 3 0.08645463961375555 2307 4 0.1314119881904645 2307 5 0.06894745554356518 2307 6 0.12791095551150988 2307 7 0.1735686965687364 2307 8 0.1569663834285535 2307 9 0.13412642808550856 2307 10-11 0.1542566268369896 2307 12-13 0.12883423168199215 2307 14-15 0.24918017615954113 2307 16-17 0.3247264942243646 2307 18-19 0.3642348146106684 2307 20-21 0.48524811356583086 2307 22-23 0.5574700328760187 2307 24-25 0.606161714420729 2307 26-27 0.6585904733148524 2307 28-29 0.6810911769098666 2307 30-31 0.6789146631434235 2307 32-33 0.4984350122000336 2307 34-35 0.4768754210772883 2307 36-37 0.5332361136489325 2307 38-39 0.6600109208583547 2307 40-41 0.5146895665498263 2307 42-43 0.4749268481004094 2307 44-45 0.5786412173358215 2307 46-47 0.4202480866359153 2307 48-49 0.49340715256357726 2307 50-51 0.6190729852568282 2307 52-53 0.6118464191770627 2307 54-55 0.5894014079495165 2307 56-57 0.6681034974337905 2307 58-59 0.47543893194903575 2307 60-61 0.4574372845580541 2307 62-63 0.3762969709403805 2307 64-65 0.5755171885888117 2307 66-67 0.6285409016712009 2307 68-69 0.523461899937594 2307 70-71 0.5710024596297103 2307 72-73 0.5674160208379604 2307 74-75 0.6936361138432403 2307 76-77 0.2399239256301975 2307 78-79 0.4937828471476564 2307 80-81 0.7479511246613768 2307 82-83 0.7095720306507687 2307 84-85 0.7946804212499288 2307 86-87 0.8229884231208544 2307 88-89 0.7308380012271698 2307 90-91 0.7636565590784095 2307 92-93 0.8097970280539926 2307 94-95 0.8874634437378397 2307 96-97 1.0494458751115623 2307 98-99 0.9776197841439789 2307 100-101 0.6395674319877287 2201 1 0.11895886677759648 2201 2 0.11207977781411671 2201 3 0.07377236984093116 2201 4 0.12053591807593023 2201 5 0.06154456815532683 2201 6 0.10455963250190337 2201 7 0.16218819231539783 2201 8 0.12625918123001867 2201 9 0.06111907853434673 2201 10-11 0.06982086082933847 2201 12-13 0.0660706015641992 2201 14-15 0.1291831593721895 2201 16-17 0.2158057844005512 2201 18-19 0.28735109662370206 2201 20-21 0.3603229198830107 2201 22-23 0.3919401510293241 2201 24-25 0.28923514665208216 2201 26-27 0.40147596314933054 2201 28-29 0.3823201701207921 2201 30-31 0.3739259179911443 2201 32-33 0.40000797886941086 2201 34-35 0.44551462410750986 2201 36-37 0.3458135189771312 2201 38-39 0.4122248262453141 2201 40-41 0.3368774366263878 2201 42-43 0.3143029950552716 2201 44-45 0.396518797136757 2201 46-47 0.40498126990461714 2201 48-49 0.4681533534874731 2201 50-51 0.5081716030349668 2201 52-53 0.26242859764549564 2201 54-55 0.41033949272699033 2201 56-57 0.3705081476295078 2201 58-59 0.46043111231588085 2201 60-61 0.46457394281749487 2201 62-63 0.41776995478583245 2201 64-65 0.34438691259644116 2201 66-67 0.33255693869919867 2201 68-69 0.25829678363749764 2201 70-71 0.37496742044114484 2201 72-73 0.28969835931507504 2201 74-75 0.4797790910894548 2201 76-77 0.36186907067761 2201 78-79 0.5321804099533125 2201 80-81 0.5030361462218735 2201 82-83 0.6319922297124023 2201 84-85 0.6443658279160545 2201 86-87 0.473511841340148 2201 88-89 0.61795549460534 2201 90-91 0.6401926875915969 2201 92-93 0.7889475356521416 2201 94-95 0.8931631879047508 2201 96-97 0.8636658554042747 2201 98-99 0.7894703703904646 2201 100-101 0.8534719149427339 1101 1 0.09689510259123324 1101 2 0.09436052055599475 1101 3 0.052133744104260415 1101 4 0.11401043773857822 1101 5 0.09932397658109693 1101 6 0.058349847078360995 1101 7 0.08683997036153812 1101 8 -0.025490566220632616 1101 9 0.040104669236583845 1101 10-11 0.04073638924155176 1101 12-13 0.026773758184283736 1101 14-15 0.10815246187530647 1101 16-17 0.1988300258475988 1101 18-19 0.1893726431141758 1101 20-21 0.31910900482515103 1101 22-23 0.34615764290990825 1101 24-25 0.307085696666725 1101 26-27 0.44656021756112096 1101 28-29 0.4535262536809057 1101 30-31 0.4435573111093021 1101 32-33 0.4258701839954213 1101 34-35 0.42171888141498215 1101 36-37 0.4257755789900912 1101 38-39 0.46871230078472337 1101 40-41 0.4863677311630994 1101 42-43 0.5172880132026307 1101 44-45 0.3683122430286758 1101 46-47 0.54194922986526 1101 48-49 0.32485701685152435 1101 50-51 0.4437848455053768 1101 52-53 0.559177703439822 1101 54-55 0.5176695113843834 1101 56-57 0.43350711768883343 1101 58-59 0.46456996016436136 1101 60-61 0.586305855512741 1101 62-63 0.48743935370192304 1101 64-65 0.3897676451376313 1101 66-67 0.3491251632196892 1101 68-69 0.42856868139367776 1101 70-71 0.42328019335074174 1101 72-73 0.5709432384039914 1101 74-75 0.5195894240597383 1101 76-77 0.2038898274372123 1101 78-79 0.4647656926605137 1101 80-81 0.7614923540000618 1101 82-83 0.8841847200299711 1101 84-85 0.8328967968829204 1101 86-87 0.8776615479880334 1101 88-89 0.9467255545919855 1101 90-91 0.778081342120533 1101 92-93 0.6861541917671232 1101 94-95 0.8272874586376595 1101 96-97 0.941871915930566 1101 98-99 0.9952648560946926 1101 100-101 0.7318362506968157 2304 1 0.07701739484049597 2304 2 0.08215902156664612 2304 3 0.11734395440399936 2304 4 0.1071487156299824 2304 5 0.038947619351780816 2304 6 0.07529248122121146 2304 7 0.07302649138256356 2304 8 0.10495072336335909 2304 9 0.08011886014605096 2304 10-11 -0.042054002677978986 2304 12-13 -0.029289427138266433 2304 14-15 0.038652631808467675 2304 16-17 0.1371664994662254 2304 18-19 0.17812575110222184 2304 20-21 0.20713289086856435 2304 22-23 0.1744090306973689 2304 24-25 0.1943867705250426 2304 26-27 0.18674255286011743 2304 28-29 0.1377962566025559 2304 30-31 0.04474222223723956 2304 32-33 0.1802547576420679 2304 34-35 0.0839806023310743 2304 36-37 0.10837084590441037 2304 38-39 0.154690430088948 2304 40-41 0.23075710467617938 2304 42-43 0.1927504102738169 2304 44-45 0.22753305477255736 2304 46-47 0.11336036078534306 2304 48-49 0.10185924685158909 2304 50-51 0.04986114847532974 2304 52-53 0.15017467445578347 2304 54-55 0.1347943019492206 2304 56-57 0.3258110833921535 2304 58-59 0.27618548788135655 2304 60-61 0.3292589956986518 2304 62-63 0.40650033883724745 2304 64-65 0.33999496800466744 2304 66-67 0.30310025757731296 2304 68-69 0.21068813546104792 2304 70-71 0.2776231780925329 2304 72-73 0.4870160830850878 2304 74-75 0.4265984215733454 2304 76-77 0.1285281977483983 2304 78-79 0.3977920531692405 2304 80-81 0.5053409226858534 2304 82-83 0.505516548808906 2304 84-85 0.5890683518607673 2304 86-87 0.6646047187619146 2304 88-89 0.4470455953759398 2304 90-91 0.6151685088875745 2304 92-93 0.6379835073240585 2304 94-95 0.715891949643126 2304 96-97 0.7930258217100778 2304 98-99 0.8441508995141049 2304 100-101 0.8011515393148656 2202 1 0.08286223081101696 2202 2 0.08252748002622923 2202 3 0.06341809497931195 2202 4 0.03627376706453589 2202 5 0.05564608192612752 2202 6 0.0533533049222541 2202 7 0.01810186638724076 2202 8 0.0654789095003423 2202 9 0.13389509176988668 2202 10-11 0.019023130453355463 2202 12-13 0.07553513426525171 2202 14-15 -0.012893210409018252 2202 16-17 -0.00478258967453371 2202 18-19 0.10487359085085046 2202 20-21 0.17123531109824341 2202 22-23 0.2055213263619038 2202 24-25 0.01244217021830707 2202 26-27 0.22133650041557473 2202 28-29 0.16660989517660596 2202 30-31 0.23787337100468164 2202 32-33 0.15518836030544492 2202 34-35 0.09827379800683644 2202 36-37 0.12970777030495384 2202 38-39 0.10291172960639727 2202 40-41 0.22953109188300402 2202 42-43 0.18166579575244413 2202 44-45 0.25514873576607755 2202 46-47 0.25879577768579765 2202 48-49 0.045575814389835045 2202 50-51 0.2841549052553418 2202 52-53 0.19398288902205252 2202 54-55 0.2635265804065554 2202 56-57 0.34434716239562846 2202 58-59 0.2424172987518105 2202 60-61 0.15976249324850045 2202 62-63 0.10535662133453627 2202 64-65 0.06622378631911374 2202 66-67 0.35280380749688334 2202 68-69 0.24997104422549654 2202 70-71 0.11980606067211141 2202 72-73 0.007858300569090204 2202 74-75 0.032228167580157674 2202 76-77 -0.17032060335884935 2202 78-79 0.12706299170962154 2202 80-81 0.40238483844540696 2202 82-83 0.5957636217150508 2202 84-85 0.5557462406124998 2202 86-87 0.5545964892328712 2202 88-89 0.6680299592707541 2202 90-91 0.6303220644181913 2202 92-93 0.6747769835519364 2202 94-95 0.6461457558003083 2202 96-97 0.7792385550359313 2202 98-99 0.7628291249283841 2202 100-101 0.47643770248398454 1102 1 0.038333812804545175 1102 2 0.0717795673015118 1102 3 0.03967768022314999 1102 4 0.017587042768191452 1102 5 -0.003996486049899772 1102 6 0.028458405243135587 1102 7 -0.01692151065938674 1102 8 -0.046610882801488174 1102 9 0.01922353072731653 1102 10-11 -0.0546000171512091 1102 12-13 -0.03572625872592994 1102 14-15 -0.1069473584435201 1102 16-17 -0.06822088147332295 1102 18-19 -0.01169501370642223 1102 20-21 -0.025997016807480122 1102 22-23 0.05868913987512059 1102 24-25 0.09146910019774168 1102 26-27 0.03458965269773984 1102 28-29 -0.11331123320691461 1102 30-31 0.019550596807235365 1102 32-33 0.011270757211100602 1102 34-35 0.03197584632642503 1102 36-37 0.0906824334710663 1102 38-39 0.1861939586134369 1102 40-41 0.23598569560078175 1102 42-43 -0.006945541478643236 1102 44-45 0.07228054273503659 1102 46-47 0.0188615113074988 1102 48-49 0.03327331169639791 1102 50-51 0.026711860208607163 1102 52-53 0.11609734961861307 1102 54-55 -0.002944107534062823 1102 56-57 0.05292319581239724 1102 58-59 -0.03252506793500487 1102 60-61 -0.0423452965057578 1102 62-63 0.0018250759615341394 1102 64-65 0.05246453752724989 1102 66-67 0.13358479799693157 1102 68-69 0.22003827642245 1102 70-71 0.18626459996861655 1102 72-73 0.1686130514527875 1102 74-75 -0.10819288114609549 1102 76-77 -0.16691261769861043 1102 78-79 0.15399897271991136 1102 80-81 0.27970499304598917 1102 82-83 0.3256543421150937 1102 84-85 0.3844503585663759 1102 86-87 0.3528751645796362 1102 88-89 0.47882046499626085 1102 90-91 0.455490526829049 1102 92-93 0.4440611374279797 1102 94-95 0.41276521007157285 1102 96-97 0.5090050610058618 1102 98-99 0.5329655025518925 1102 100-101 0.34405094494255195 2305 1 0.015671282992087754 2305 2 0.0030149836870805302 2305 3 0.003073359307890655 2305 4 0.0020233103517028894 2305 5 -0.03896612447154979 2305 6 -0.03240174636819404 2305 7 0.009395342012169294 2305 8 -0.034992015705825 2305 9 -0.06508583688218295 2305 10-11 -0.004836017561977712 2305 12-13 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-0.25587473254958226 1213 78-79 -0.5470341522463116 1213 80-81 -0.7156382740812397 1213 82-83 -0.8234542518999994 1213 84-85 -0.9319931478329373 1213 86-87 -1.051056712988391 1213 88-89 -1.2901313494357325 1213 90-91 -1.214727611380436 1213 92-93 -1.1547951186093108 1213 94-95 -1.0861100272643043 1213 96-97 -1.3260863640346336 1213 98-99 -1.4252982063752775 1213 100-101 -0.9550582898306672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 14.0 9 81.0 10 126.0 11 119.0 12 161.0 13 434.0 14 1385.0 15 3540.0 16 7007.0 17 11003.0 18 13784.0 19 15122.0 20 17499.0 21 19981.0 22 22721.0 23 26119.0 24 30246.0 25 34304.0 26 39482.0 27 46425.0 28 54585.0 29 65050.0 30 76901.0 31 91707.0 32 115818.0 33 152426.0 34 221237.0 35 365451.0 36 707530.0 37 924384.0 38 249619.0 39 56.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.62484063749233 31.534693810947946 20.32602872105978 15.514436830499937 2 30.15726609297415 24.979460335592318 31.385421861926986 13.477851709506538 3 33.2654526801744 22.886984596352004 26.341807099859693 17.505755623613897 4 29.350211158893597 23.162543465902576 30.033927845093018 17.453317530110812 5 29.213271263267053 21.44781476979132 31.226988108265978 18.111925858675644 6 27.364505347855772 22.2176260040147 32.20378002238167 18.214088625747856 7 30.864017615685153 22.236332712693017 26.62190935754826 20.27774031407357 8 27.431819326986933 26.990461690621814 26.0354842125939 19.542234769797357 9 24.78086750249448 24.714669891461867 30.63470957382195 19.869753032221702 10-11 19.822096183258157 30.211832355304363 34.621033762893674 15.345037698543804 12-13 19.850889361082636 29.78450314431252 34.45293035443949 15.91167714016536 14-15 20.098050308374056 29.64557726640073 34.57674110428115 15.679631320944063 16-17 19.92938820916152 29.507223052458137 34.50034531980778 16.063043418572565 18-19 19.270006900361732 29.817211318524254 34.1406032430795 16.77217853803451 20-21 19.23604818968844 30.034239311303466 33.72686817412566 17.002844324882428 22-23 19.173671889166773 29.670583315456327 33.79404721280096 17.361697582575935 24-25 19.17605962615229 29.130145195805774 34.105256964669195 17.588538213372736 26-27 19.247392903338515 29.54065375119293 33.89473071240276 17.3172226330658 28-29 19.050987548271607 29.935018325031475 33.74376455615769 17.27022957053923 30-31 18.372612895741177 31.00122528941843 33.56778572008307 17.058376094757325 32-33 18.156852131614368 31.391380853804357 33.57063698455097 16.881130030030302 34-35 18.499863772919866 30.870082209950215 34.071131958028175 16.558922059101732 36-37 18.629181439686405 30.462472079482993 34.42069094737109 16.487655533459513 38-39 18.96558747024624 30.27805075420445 33.75892009352238 16.997441682026935 40-41 18.77343430128482 30.150220905109016 33.29427553593966 17.782069257666507 42-43 18.98472959301745 29.814888065994854 33.2436769061759 17.9567054348118 44-45 18.63643961529317 29.63041242898987 33.47158207002591 18.261565885691045 46-47 18.759573138894297 29.975727807478318 33.343209767773544 17.921489285853834 48-49 18.667416765030328 30.627487755836043 32.831195884898825 17.873899594234803 50-51 19.21921790541305 30.528436580218177 31.885915750059212 18.36642976430956 52-53 19.125205739432424 30.435797982358082 31.950281585811634 18.488714692397863 54-55 19.505062552859073 30.524280550847756 31.92549295659906 18.045163939694113 56-57 19.475199551984897 30.60620836443711 31.76954499069753 18.14904709288046 58-59 19.224479740046917 31.201671989068647 31.67208109865119 17.90176717223325 60-61 19.110353512076486 31.068728074932906 31.508809703731274 18.312108709259334 62-63 18.674948989790416 30.9491255662039 31.896719883232823 18.479205560772865 64-65 18.615131560585787 30.580672102451473 32.20537303597488 18.598823300987863 66-67 18.898477220926306 30.504811424245244 31.870908599610836 18.725802755217615 68-69 19.23062358152286 30.612990327462562 31.750367033199474 18.406019057815104 70-71 18.83470282102092 30.81457435441563 31.96134112931965 18.389381695243802 72-73 18.790808504372368 30.704953098979605 31.762251490371312 18.741986906276715 74-75 19.102306514447005 30.348852068624073 31.330120625166995 19.218720791761925 76-77 19.117786833184226 30.04722154750895 31.00987729941026 19.825114319896564 78-79 18.830686935858097 30.241936153042726 31.221098441399697 19.70627846969948 80-81 18.553585930337512 30.644822820476964 30.827956176530357 19.973635072655167 82-83 18.14718197011211 30.89775003552766 30.597145295911467 20.35792269844876 84-85 18.084432962877813 31.022949656307308 31.056682289884613 19.835935090930263 86-87 18.32738178751548 30.736211497873583 31.35653003003683 19.579876684574106 88-89 18.706473666384294 30.63849560559509 31.218899524238058 19.436131203782562 90-91 18.590507226698048 30.66266859780936 30.76020639087535 19.98661778461724 92-93 18.187422460260954 30.79919548018098 30.689751575613535 20.32363048394453 94-95 18.003733792146605 30.652082930378082 30.65436110483314 20.689822172642174 96-97 18.02201433172072 31.144387673120892 30.375658857068284 20.457939138090104 98-99 18.561111309759006 30.92956805548703 30.018528568258105 20.49079206649585 100-101 18.665457280891616 30.90298994079501 29.618530517830145 20.81302226048323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 158042.0 1 86879.0 2 13382.5 3 11418.5 4 10374.5 5 8407.5 6 8066.5 7 8473.5 8 8596.5 9 8139.0 10 7234.0 11 6393.0 12 5890.0 13 5469.5 14 5229.5 15 5069.0 16 4967.5 17 5014.0 18 4998.5 19 5165.5 20 5376.5 21 5562.5 22 5976.5 23 6695.5 24 7582.5 25 8982.0 26 10924.0 27 13462.5 28 16648.5 29 20049.0 30 24414.0 31 30029.0 32 38761.5 33 53648.5 34 74015.5 35 95415.0 36 123648.0 37 161470.5 38 189751.0 39 193936.5 40 192811.0 41 191803.0 42 183704.5 43 178302.5 44 168244.5 45 155491.0 46 151005.5 47 140491.5 48 117305.5 49 94296.0 50 75454.0 51 60435.5 52 49091.5 53 38666.0 54 30645.0 55 25433.0 56 20141.0 57 14951.0 58 10793.0 59 7653.5 60 5549.5 61 4263.5 62 3279.0 63 2491.5 64 1961.5 65 1490.5 66 1000.0 67 677.5 68 505.5 69 405.5 70 312.5 71 239.5 72 195.0 73 161.0 74 121.5 75 88.0 76 76.0 77 58.5 78 40.0 79 33.5 80 25.5 81 18.0 82 17.5 83 13.5 84 10.0 85 7.0 86 6.0 87 5.5 88 2.0 89 1.0 90 2.0 91 1.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15206743828822755 2 0.0 3 0.005099086720379058 4 0.006849069145124534 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.5258166157210576E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.810326646288423E-4 24-25 4.5258166157210576E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.65507544687159E-4 40-41 0.0 42-43 0.0 44-45 9.051633231442115E-5 46-47 9.80593600072896E-4 48-49 0.0026249736371182137 50-51 4.2240955080063205E-4 52-53 0.0016292939816595809 54-55 0.002474113083260845 56-57 0.004374956061863689 58-59 0.005506410215793954 60-61 0.0014331752616450015 62-63 9.504214893014222E-4 64-65 0.0014331752616450015 66-67 0.008433104960626905 68-69 0.007407253194396798 70-71 0.009232665896070958 72-73 0.006396487483552428 74-75 0.008825342400656062 76-77 0.004646505058806953 78-79 0.004812451668050058 80-81 0.002036617477074476 82-83 0.0 84-85 6.336143262009481E-4 86-87 0.0016292939816595809 88-89 0.004872795889593005 90-91 0.006607692258952745 92-93 0.008825342400656062 94-95 0.007905093022126114 96-97 0.010303775828458275 98-99 0.007467597415939745 100-101 0.007241306585153692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3314319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.282100443863925 #Duplication Level Percentage of deduplicated Percentage of total 1 80.46460169439368 34.02212371017857 2 9.415580027413112 7.962210009126405 3 3.2399585197091914 4.109767546928902 4 1.708943231776086 2.890308375152716 5 0.9974579098787217 2.1087307767009342 6 0.7473823542440775 1.8960537463271747 7 0.5029619448579228 1.4886401230346684 8 0.3480671169800602 1.1773607041085632 9 0.35527622076447346 1.3519642366511855 >10 1.9303106490554458 15.056716259142949 >50 0.14581379197734884 4.310605158971648 >100 0.13112933803738955 11.468276684341136 >500 0.010152971151015718 2.783917504697138 >1k 0.002230506741089684 1.2761591575377345 >5k 0.0 0.0 >10k+ 1.33723020415254E-4 8.097166007100116 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177659 5.360347027549249 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108759 3.2814885953947104 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 3758 0.11338679227919823 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.051633231442115E-5 0.0 0.0 2 1.810326646288423E-4 0.0 0.0 3 2.4137688617178975E-4 0.0 0.0 4 2.4137688617178975E-4 0.0 0.0 5 3.620653292576846E-4 0.0 0.0 6 3.922374400291583E-4 0.0 0.0 7 4.5258166157210577E-4 0.0 0.0 8 7.241306585153692E-4 0.0 0.0 9 9.65507544687159E-4 0.0 0.0 10-11 0.0024137688617178973 0.0 0.0 12-13 0.00989645233304338 0.0 0.0 14-15 0.02351916034636376 0.0 0.0 16-17 0.058533894896659014 0.0 0.0 18-19 0.09819513450576121 0.0 0.0 20-21 0.11046009753436528 0.0 0.0 22-23 0.11952681682119314 0.0 0.0 24-25 0.12936292493269358 0.0 0.0 26-27 0.14036065930889574 0.0 0.0 28-29 0.152384245451328 0.0 0.0 30-31 0.16582592080001957 0.0 0.0 32-33 0.18077620168728478 0.0 0.0 34-35 0.19525881485759217 0.0 0.0 36-37 0.21155175467418796 0.0 0.0 38-39 0.22974553746938664 0.0 0.0 40-41 0.24987033535395958 0.0 0.0 42-43 0.2708399523401338 0.0 0.0 44-45 0.2921716346555657 0.0 0.0 46-47 0.31537398783882903 0.0 0.0 48-49 0.3401905489483662 0.0 0.0 50-51 0.36674200642726307 0.0 0.0 52-53 0.3962654168171501 0.0 0.0 54-55 0.4260754622593661 0.0 0.0 56-57 0.45600619614466803 0.0 0.0 58-59 0.48936146460253227 0.0 0.0 60-61 0.5258546325806297 0.0 0.0 62-63 0.5649727741958455 0.0 0.0 64-65 0.6067762336697222 0.0 0.0 66-67 0.6538598125286069 0.0 0.0 68-69 0.7034175044707525 0.0 0.0 70-71 0.7558868051023453 0.0 0.0 72-73 0.8146922489959476 0.0 0.0 74-75 0.8793209102684443 0.0 0.0 76-77 0.9540723147047705 0.0 0.0 78-79 1.0371964798801805 0.0 0.0 80-81 1.1338075785704393 0.0 0.0 82-83 1.246364637803422 0.0 0.0 84-85 1.3731478472651546 0.0 0.0 86-87 1.5201011127776174 0.0 0.0 88-89 1.687993823165483 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 25 0.004990155 57.09199 1 ATACGCG 25 0.004990155 57.09199 1 CGCGGAG 110 0.0 51.901806 1 CAGCCGG 70 7.8271114E-8 47.57666 1 CGGGTCG 60 1.4766465E-6 47.576656 1 ACGGAGC 70 4.2651354E-6 40.779995 1 CGGGCCG 70 4.2651354E-6 40.779995 1 CGATGCG 50 0.0016035015 38.06133 1 ACTGGCG 265 0.0 37.642532 2 CGTGCGG 80 1.0663693E-5 35.682495 1 CGCGTTG 80 1.0785765E-5 35.62328 6 CTGCGGA 135 3.0377123E-10 35.24197 1 CGAGGGG 585 0.0 34.97088 1 CGCGAGG 55 0.0025599992 34.601204 1 CGCGGGG 250 0.0 34.255196 1 GGCCGGC 115 1.0371514E-7 33.044376 2 ACCGGGC 130 8.167262E-9 32.937687 1 CGTGGCT 205 0.0 32.49138 1 CGAGCGG 90 2.38786E-5 31.717775 1 CGGACGG 90 2.38786E-5 31.717775 1 >>END_MODULE