FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314319
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1776595.360347027549249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1087593.2814885953947104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT37580.11338679227919823TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG250.00499015557.091991
ATACGCG250.00499015557.091991
CGCGGAG1100.051.9018061
CAGCCGG707.8271114E-847.576661
CGGGTCG601.4766465E-647.5766561
ACGGAGC704.2651354E-640.7799951
CGGGCCG704.2651354E-640.7799951
CGATGCG500.001603501538.061331
ACTGGCG2650.037.6425322
CGTGCGG801.0663693E-535.6824951
CGCGTTG801.0785765E-535.623286
CTGCGGA1353.0377123E-1035.241971
CGAGGGG5850.034.970881
CGCGAGG550.002559999234.6012041
CGCGGGG2500.034.2551961
GGCCGGC1151.0371514E-733.0443762
ACCGGGC1308.167262E-932.9376871
CGTGGCT2050.032.491381
CGAGCGG902.38786E-531.7177751
CGGACGG902.38786E-531.7177751
Produced by FastQC (version 0.11.2)