##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5873284 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68483407238608 33.0 33.0 33.0 33.0 33.0 2 32.716783319178845 33.0 33.0 33.0 33.0 33.0 3 32.74663203754493 33.0 33.0 33.0 33.0 33.0 4 36.68016530445318 37.0 37.0 37.0 37.0 37.0 5 36.668438474965626 37.0 37.0 37.0 37.0 37.0 6 36.54695396987444 37.0 37.0 37.0 37.0 37.0 7 36.487573902436864 37.0 37.0 37.0 37.0 37.0 8 36.41246021816755 37.0 37.0 37.0 37.0 37.0 9 36.40121557207178 37.0 37.0 37.0 37.0 37.0 10-11 36.34345078494417 37.0 37.0 37.0 37.0 37.0 12-13 36.36406821124264 37.0 37.0 37.0 37.0 37.0 14-15 38.2242287279144 40.0 37.0 40.0 37.0 40.0 16-17 38.19453741722688 40.0 37.0 40.0 37.0 40.0 18-19 38.18722941032649 40.0 37.0 40.0 37.0 40.0 20-21 38.11664343151123 40.0 37.0 40.0 37.0 40.0 22-23 38.01440829355434 40.0 37.0 40.0 35.0 40.0 24-25 37.9769032112188 40.0 37.0 40.0 33.0 40.0 26-27 37.88454704386847 40.0 37.0 40.0 33.0 40.0 28-29 37.76663013401021 40.0 37.0 40.0 33.0 40.0 30-31 37.72448310008507 40.0 37.0 40.0 33.0 40.0 32-33 37.579353475840776 40.0 37.0 40.0 33.0 40.0 34-35 37.475246727384544 40.0 37.0 40.0 33.0 40.0 36-37 37.37324544496742 40.0 37.0 40.0 33.0 40.0 38-39 37.29488102397228 40.0 37.0 40.0 33.0 40.0 40-41 37.19877780471709 40.0 37.0 40.0 33.0 40.0 42-43 37.06063703032239 40.0 37.0 40.0 33.0 40.0 44-45 36.91744329067009 40.0 37.0 40.0 33.0 40.0 46-47 36.828889936192425 37.0 37.0 40.0 33.0 40.0 48-49 36.628083538953675 37.0 37.0 40.0 33.0 40.0 50-51 36.466481018115246 37.0 37.0 40.0 33.0 40.0 52-53 36.305711166018874 37.0 37.0 40.0 33.0 40.0 54-55 36.12780430845844 37.0 37.0 40.0 33.0 40.0 56-57 35.95482510295773 37.0 37.0 40.0 30.0 40.0 58-59 35.71777841834313 37.0 37.0 40.0 27.0 40.0 60-61 35.435554367880044 37.0 35.0 40.0 27.0 40.0 62-63 35.11746673581594 37.0 33.0 38.5 27.0 40.0 64-65 34.815671777492796 37.0 33.0 37.0 27.0 40.0 66-67 34.542147885237625 37.0 33.0 37.0 27.0 40.0 68-69 34.29092608155846 37.0 33.0 37.0 27.0 40.0 70-71 33.955671222437054 37.0 33.0 37.0 27.0 40.0 72-73 33.678203284567886 37.0 33.0 37.0 27.0 37.0 74-75 33.369634347666484 37.0 33.0 37.0 27.0 37.0 76-77 30.54909136013174 33.0 30.0 35.0 24.5 37.0 78-79 33.09595338825774 35.0 33.0 37.0 27.0 37.0 80-81 33.52901043096162 37.0 33.0 37.0 27.0 37.0 82-83 33.49030636012152 37.0 33.0 37.0 27.0 37.0 84-85 33.346367381519435 37.0 33.0 37.0 27.0 37.0 86-87 33.201272746218294 37.0 33.0 37.0 27.0 37.0 88-89 33.06778524586926 37.0 33.0 37.0 27.0 37.0 90-91 32.92528098419896 37.0 33.0 37.0 27.0 37.0 92-93 32.818380313296615 37.0 33.0 37.0 27.0 37.0 94-95 32.65032807880566 33.0 33.0 37.0 27.0 37.0 96-97 32.49873775897777 33.0 33.0 37.0 24.5 37.0 98-99 32.3588038650949 33.0 33.0 37.0 22.0 37.0 100-101 30.713436128748413 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15624956377202892 2306 2 0.14646838178219923 2306 3 0.12185794689573726 2306 4 0.15352859177082934 2306 5 0.14725835324144754 2306 6 0.1799134968901157 2306 7 0.1859490382661093 2306 8 0.19500027027969935 2306 9 0.17270862370374118 2306 10-11 0.22074284616405748 2306 12-13 0.21414887953539363 2306 14-15 0.47007703790118427 2306 16-17 0.3892068715486374 2306 18-19 0.3935507377896883 2306 20-21 0.34318234075561804 2306 22-23 0.35486344458447405 2306 24-25 0.4094471384656515 2306 26-27 0.4237644854939404 2306 28-29 0.45176457466626374 2306 30-31 0.4919875544450818 2306 32-33 0.5059961791114205 2306 34-35 0.525859296770868 2306 36-37 0.496787366200401 2306 38-39 0.5144713881118577 2306 40-41 0.41970467573347037 2306 42-43 0.5034427928539245 2306 44-45 0.49526273094461715 2306 46-47 0.45047001128510544 2306 48-49 0.44670412873758636 2306 50-51 0.44900341529850607 2306 52-53 0.47864535460234947 2306 54-55 0.47209108883610185 2306 56-57 0.35651188584800053 2306 58-59 0.4161932332052487 2306 60-61 0.36320906567575406 2306 62-63 0.41935169519639715 2306 64-65 0.47041201409480493 2306 66-67 0.454820177701194 2306 68-69 0.3909200088672051 2306 70-71 0.577769982172363 2306 72-73 0.41634337479892025 2306 74-75 0.37453507923770957 2306 76-77 0.08909577679898462 2306 78-79 0.21146055050031975 2306 80-81 0.43460660937769546 2306 82-83 0.5308682876145667 2306 84-85 0.5396046473174039 2306 86-87 0.4332207648411597 2306 88-89 0.4376254556684316 2306 90-91 0.38506393836016883 2306 92-93 0.40261495195448305 2306 94-95 0.32972710432301255 2306 96-97 0.505116930779522 2306 98-99 0.5400080272755758 2306 100-101 0.29132102457015563 2307 1 0.14262657757991093 2307 2 0.1462653807986456 2307 3 0.14589879107485615 2307 4 0.17122208125289973 2307 5 0.12954199040911618 2307 6 0.1555257402320649 2307 7 0.2225721312005291 2307 8 0.21713023665587627 2307 9 0.24493027409220502 2307 10-11 0.20422352199531701 2307 12-13 0.19549185017363158 2307 14-15 0.3969028077800303 2307 16-17 0.37270994420202896 2307 18-19 0.34510259929918163 2307 20-21 0.36457273547339497 2307 22-23 0.3800231768137863 2307 24-25 0.442085026645465 2307 26-27 0.43514731294501274 2307 28-29 0.41733160501919286 2307 30-31 0.4631647504773255 2307 32-33 0.46257112364354924 2307 34-35 0.4372693816310047 2307 36-37 0.38595597710184393 2307 38-39 0.43024122883709737 2307 40-41 0.4431608198965691 2307 42-43 0.2657062442899871 2307 44-45 0.3346255747120068 2307 46-47 0.28070495871451584 2307 48-49 0.3040611512373417 2307 50-51 0.3035432034912873 2307 52-53 0.2710849498965402 2307 54-55 0.28500995706272647 2307 56-57 0.3314512714698168 2307 58-59 0.37071052927026926 2307 60-61 0.27241711404526825 2307 62-63 0.20153878776484646 2307 64-65 0.14946507645518636 2307 66-67 0.2710633061655585 2307 68-69 0.22948895676933745 2307 70-71 0.2876158157916109 2307 72-73 0.20889018892245304 2307 74-75 0.15843529043498705 2307 76-77 -0.2934324146052134 2307 78-79 0.007763835875870484 2307 80-81 0.2276213741252846 2307 82-83 0.20100264948621316 2307 84-85 0.22975747941938351 2307 86-87 0.14385811168502016 2307 88-89 0.16416931150533287 2307 90-91 0.23005982115711987 2307 92-93 0.2316423771014442 2307 94-95 0.34441894311164134 2307 96-97 0.3482972413996208 2307 98-99 0.27697260026825177 2307 100-101 0.19243857834159073 2201 1 0.09488965403763672 2201 2 0.1155865063357453 2201 3 0.10597623379748455 2201 4 0.0980870858288796 2201 5 0.11337790645918489 2201 6 0.07368987847333841 2201 7 0.16685932464833542 2201 8 0.12329269186551528 2201 9 0.16184590534540177 2201 10-11 0.15220247828303002 2201 12-13 0.15358954938407976 2201 14-15 0.22380353799858455 2201 16-17 0.2166489944137595 2201 18-19 0.16762230899561104 2201 20-21 0.22275812489744595 2201 22-23 0.2569948735733689 2201 24-25 0.19553795605121138 2201 26-27 0.18230501572318047 2201 28-29 0.17340132867626323 2201 30-31 0.16187358600557644 2201 32-33 0.27828090539235006 2201 34-35 0.21259808662735935 2201 36-37 0.1966061450172134 2201 38-39 0.20551869637862552 2201 40-41 0.22590800062469185 2201 42-43 0.23781576350137357 2201 44-45 0.2648654648883735 2201 46-47 0.21754924532626774 2201 48-49 0.26149019439120735 2201 50-51 0.26299275713888903 2201 52-53 0.21511139947539704 2201 54-55 0.28568937443905895 2201 56-57 0.24640713066909115 2201 58-59 0.21410259420271416 2201 60-61 0.2845680013209275 2201 62-63 0.16925448920899555 2201 64-65 0.14163437431300707 2201 66-67 0.2516316589662111 2201 68-69 0.12129901075044103 2201 70-71 0.25358963766947085 2201 72-73 0.12838616166819605 2201 74-75 0.07594832035726995 2201 76-77 -0.030037770833981625 2201 78-79 0.1598733938667749 2201 80-81 0.3364368824473729 2201 82-83 0.3984477570606373 2201 84-85 0.33908919176833763 2201 86-87 0.25934462028632055 2201 88-89 0.3104930120032563 2201 90-91 0.2919864657970166 2201 92-93 0.2424328784432177 2201 94-95 0.21834649519254867 2201 96-97 0.41616777011974193 2201 98-99 0.14957398935103328 2201 100-101 0.13655407541761733 1101 1 0.10021472403995801 1101 2 0.11578981284366563 1101 3 0.09221777885641558 1101 4 0.14960143718766972 1101 5 0.13387889037099399 1101 6 0.12053950924191525 1101 7 0.12047907976361927 1101 8 -0.00875761811960274 1101 9 0.07658836025826332 1101 10-11 0.09605591686083415 1101 12-13 0.11032747177848279 1101 14-15 0.15493418579342944 1101 16-17 0.14638195404869236 1101 18-19 0.13981924035454085 1101 20-21 0.13679517852239798 1101 22-23 0.10736170252083355 1101 24-25 0.17602864239140814 1101 26-27 0.17887465922266443 1101 28-29 0.1427302364636276 1101 30-31 0.24552910049623478 1101 32-33 0.23375034417470175 1101 34-35 0.21749888874470713 1101 36-37 0.10316632850442176 1101 38-39 0.10000782045025858 1101 40-41 0.1453130161063214 1101 42-43 0.1490458251810054 1101 44-45 -0.02216929294236536 1101 46-47 0.09618824226521383 1101 48-49 -0.009690689330874136 1101 50-51 0.03249684707336087 1101 52-53 0.1197656064918462 1101 54-55 0.01645795415002027 1101 56-57 0.038587919173899365 1101 58-59 0.09255955423262208 1101 60-61 0.07393597904714255 1101 62-63 -0.05287151409892488 1101 64-65 -0.06966577785830452 1101 66-67 0.1444371124472852 1101 68-69 0.025552181132937335 1101 70-71 -0.053798098522257476 1101 72-73 0.016261630701421836 1101 74-75 0.055766226141535924 1101 76-77 -0.18834909252024445 1101 78-79 -0.1415638799922121 1101 80-81 0.07812677301804172 1101 82-83 0.1784450327886944 1101 84-85 0.21611705516329494 1101 86-87 0.17078140483431525 1101 88-89 0.16498409331881447 1101 90-91 0.09265316302724358 1101 92-93 -0.051504734847704015 1101 94-95 -0.015778405181968935 1101 96-97 0.04853976901240031 1101 98-99 0.05044324369400499 1101 100-101 0.048411050259094424 2304 1 0.09313276967063189 2304 2 0.09326105245491334 2304 3 0.07175663129014254 2304 4 0.08098811543775497 2304 5 0.0913093470992905 2304 6 0.08471925041869355 2304 7 0.0754060598202102 2304 8 0.051860784144466265 2304 9 0.026450646555481683 2304 10-11 0.012228176196735774 2304 12-13 0.01838184220998329 2304 14-15 0.13434099416744516 2304 16-17 0.09671961502218807 2304 18-19 0.09550276842725935 2304 20-21 0.0789549013619748 2304 22-23 0.06666213762979822 2304 24-25 0.054985175904185724 2304 26-27 0.12682117232391477 2304 28-29 0.17117275178390656 2304 30-31 0.07650997364214618 2304 32-33 0.10669956561465455 2304 34-35 0.054950101506257454 2304 36-37 0.04908510098974972 2304 38-39 0.040127194544147926 2304 40-41 0.1145496412295941 2304 42-43 0.09926131980154196 2304 44-45 0.06355053048278592 2304 46-47 0.11161422880920924 2304 48-49 0.04429734242201988 2304 50-51 0.03356534591811311 2304 52-53 0.00814998950016843 2304 54-55 -0.06362557350511366 2304 56-57 0.004079146766464703 2304 58-59 -0.01946470268477185 2304 60-61 -0.059624710185509855 2304 62-63 0.047405720204217516 2304 64-65 -0.1320028408691556 2304 66-67 -0.051411813408321905 2304 68-69 -0.11106013726683273 2304 70-71 -0.05775835625170345 2304 72-73 -0.015793053568117443 2304 74-75 -0.06651221468734292 2304 76-77 -0.46504734937804315 2304 78-79 -0.028141706724859716 2304 80-81 0.06611518044500997 2304 82-83 0.21314362982507618 2304 84-85 0.23960315004244137 2304 86-87 0.1238675377145313 2304 88-89 0.11596462837410826 2304 90-91 0.11283689158529597 2304 92-93 0.038723900335092765 2304 94-95 0.1378442477885713 2304 96-97 0.10831577554870364 2304 98-99 0.0821001695765915 2304 100-101 0.19032566944981255 2202 1 0.0694937411811054 2202 2 0.06843405353470189 2202 3 0.0610370469134196 2202 4 0.06197630103554275 2202 5 0.04760157771111295 2202 6 0.065531281600407 2202 7 0.06642104859316333 2202 8 0.09768053761037265 2202 9 0.060710426790109295 2202 10-11 0.06477688543963467 2202 12-13 0.08847491931578588 2202 14-15 0.06684565417916133 2202 16-17 0.07218988355011646 2202 18-19 0.06245739827615182 2202 20-21 0.0686197271838509 2202 22-23 0.07973435639636506 2202 24-25 0.007285073905386241 2202 26-27 0.14049670255896984 2202 28-29 0.13855719547638756 2202 30-31 0.13154853595826665 2202 32-33 0.1377291458511749 2202 34-35 0.08651138810849801 2202 36-37 0.03318475550430122 2202 38-39 0.12328219306038335 2202 40-41 0.1391868719693079 2202 42-43 0.031146416111880626 2202 44-45 0.09153456686902928 2202 46-47 -0.02983749245800027 2202 48-49 -0.09025540675586541 2202 50-51 0.2182954991200532 2202 52-53 0.16781421580336797 2202 54-55 0.14535928747582005 2202 56-57 0.16016658092110703 2202 58-59 0.04651864828599628 2202 60-61 0.085484020000969 2202 62-63 -0.09234061597081933 2202 64-65 -0.08853423765457791 2202 66-67 -0.0064480047673498575 2202 68-69 -0.00836894706176139 2202 70-71 -0.007942184213597159 2202 72-73 -0.04205013904694965 2202 74-75 -0.03364523432143329 2202 76-77 -0.41963428651118306 2202 78-79 -0.20379605973244708 2202 80-81 0.04830161550691514 2202 82-83 0.15269497951530298 2202 84-85 0.12910636234116168 2202 86-87 -0.017156678369005363 2202 88-89 0.044268993155860414 2202 90-91 0.061300383133662706 2202 92-93 0.14822649845699232 2202 94-95 0.12317284228594616 2202 96-97 0.20102047251364752 2202 98-99 0.10193808112911285 2202 100-101 0.12584728990333716 1102 1 0.08396565728911298 1102 2 0.0856133371245349 1102 3 0.05683203817108762 1102 4 -0.01630214701835797 1102 5 0.03399605360681335 1102 6 0.025544044885343453 1102 7 0.0847866003759492 1102 8 -0.042196740837482594 1102 9 0.015827741084748936 1102 10-11 0.038743651931405054 1102 12-13 0.009886190034869458 1102 14-15 -0.10130559577642373 1102 16-17 -0.06975477839851862 1102 18-19 -0.047390212548478416 1102 20-21 -0.06329490716503727 1102 22-23 -0.06126874198237431 1102 24-25 -0.016684415570495048 1102 26-27 -0.027417287140806934 1102 28-29 -0.0669819497527655 1102 30-31 -0.015040170427091937 1102 32-33 0.003949383188192712 1102 34-35 -0.04140663015197532 1102 36-37 -0.0430279798956974 1102 38-39 -0.02501446623184478 1102 40-41 0.012859466844794554 1102 42-43 -0.05225107663846984 1102 44-45 0.00544810249548533 1102 46-47 -0.12468506721880601 1102 48-49 0.047145081078490136 1102 50-51 -0.014519840802662998 1102 52-53 -0.04180024074756972 1102 54-55 -0.09022403362117615 1102 56-57 -0.126090432251857 1102 58-59 -0.02433778216175142 1102 60-61 -0.04293806463758898 1102 62-63 -0.09987127170973054 1102 64-65 -0.010388133907362374 1102 66-67 -0.02864740568438151 1102 68-69 0.04363816353314576 1102 70-71 -0.07339734573088919 1102 72-73 -0.11838944523324102 1102 74-75 -0.17477210771299667 1102 76-77 -0.3247405235873444 1102 78-79 -0.0513474452218361 1102 80-81 0.06305310896279082 1102 82-83 0.13117910385621911 1102 84-85 0.10286197349162052 1102 86-87 0.040540989267725536 1102 88-89 0.03407049125370776 1102 90-91 0.09139637179757898 1102 92-93 0.07611812395410311 1102 94-95 -0.06886716711844088 1102 96-97 0.03277103962554406 1102 98-99 -0.09696161492233557 1102 100-101 -0.03049883135303233 2305 1 -0.03241533403253527 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sequence quality scores pass #Quality Count 7 10.0 8 52.0 9 207.0 10 281.0 11 241.0 12 266.0 13 528.0 14 1389.0 15 2889.0 16 5344.0 17 8006.0 18 10273.0 19 12478.0 20 15403.0 21 19016.0 22 22910.0 23 27435.0 24 32849.0 25 40113.0 26 48711.0 27 59814.0 28 75540.0 29 95449.0 30 112679.0 31 136540.0 32 180043.0 33 249578.0 34 374900.0 35 643064.0 36 1255370.0 37 1955533.0 38 486149.0 39 224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.124470513123086 28.9871981839538 16.43590360985565 16.452427693067456 2 33.376182728436085 23.44684847523123 28.41560190176399 14.761366894568695 3 39.2146679854745 20.611917525358926 21.835486952877933 18.337927536288642 4 33.25632378745297 20.61138813849799 27.273451713942006 18.858836360107034 5 35.10625401393837 18.49282275469737 26.975845200061837 19.42507803130242 6 32.061160332107214 19.554766975341224 28.73261364510894 19.651459047442625 7 35.89901322667182 20.02145647988417 22.272377770255957 21.80715252318805 8 31.061515159151163 26.019906410110593 22.1624222496307 20.75615618110754 9 29.41929932215095 22.606024159567287 26.859811308290215 21.11486520999155 10-11 22.54178411941258 30.15518234772914 31.45113534438314 15.851898188475136 12-13 22.462039950419527 29.33520111419854 31.578487659365805 16.624271276016128 14-15 22.360233218758026 29.0466628210044 31.82562770674805 16.76747625348953 16-17 22.098133011968518 28.930239788356886 31.868783449666616 17.10284375000798 18-19 21.001232019428993 29.262751469195088 31.9339061417769 17.802110369599017 20-21 21.581035413918347 29.05310725651952 31.243423611049625 18.122433718512504 22-23 20.956663464309322 28.57374779243787 31.522544269362378 18.94704447389043 24-25 20.4850557201048 27.825880716818734 32.178173233237146 19.51089032983932 26-27 20.50169036607118 28.265200524953332 31.634525080006348 19.598584028969142 28-29 20.659974896497427 28.80042919770268 30.993146253441857 19.546449652358035 30-31 19.71764859318909 30.498159121881386 30.517790387796673 19.266401897132848 32-33 19.380877887056034 31.19318766128115 31.04699176814879 18.378942683514026 34-35 20.002157225838218 30.32098396740222 32.168425705278345 17.508433101481216 36-37 19.65628598923532 29.955975226125624 33.16098795835515 17.226750826283897 38-39 20.152275664327806 29.450762187075934 31.791586571613916 18.60537557698234 40-41 20.338229855733182 29.16636586958846 31.352459714190562 19.142944560487795 42-43 20.380454955013246 28.810806696900748 31.36911138640665 19.43962696167936 44-45 20.008310522388097 28.360283364806392 31.812334068357412 19.8190720444481 46-47 20.6763810293079 28.661470319217987 31.02308474998757 19.63906390148654 48-49 20.463467458522896 29.795023769308305 30.43632685863732 19.30518191353148 50-51 21.428389003721954 29.632631621961224 28.901070274430214 20.037909099886605 52-53 21.076041654960957 29.55115454908583 28.79076864715363 20.582035148799584 54-55 21.706161145510293 29.403253347177195 29.106307285879794 19.78427822143271 56-57 21.189401153329285 30.111029333828256 29.014757796167974 19.684811716674492 58-59 20.77754690640208 31.072018871705875 28.73196446264626 19.418469759245784 60-61 20.977619801732722 31.004797568882154 28.51307374553117 19.50450888385395 62-63 20.39358240695495 30.785649463753334 29.231913274297106 19.58885485499461 64-65 20.803062320615037 29.68728424214903 29.86224018178868 19.647413255447248 66-67 21.033236093619845 29.64390580802388 29.331174443686898 19.99168365466938 68-69 21.209185238967216 30.332571404440866 29.483123422604564 18.975119933987354 70-71 20.546018835087963 30.942991476081016 29.54924809408672 18.9617415947443 72-73 20.319298469659238 30.36412319349552 29.652291254283465 19.664287082561778 74-75 20.593481890403332 29.387685252902617 29.33936858846377 20.679464268230284 76-77 20.566894234854118 28.739537865456377 28.908779466589703 21.784788433099806 78-79 19.910173599671037 29.271845114800882 29.336428857848507 21.481552427679574 80-81 19.429179861926198 29.905322744718415 28.964943679841365 21.70055371351402 82-83 18.60425104592252 30.143553419177415 28.997150486848582 22.255045048051482 84-85 18.34646588310931 30.546094828312025 30.001788615906694 21.105650672671967 86-87 18.785806247184773 30.190103690677784 30.64741908974672 20.376670972390727 88-89 19.574500183212802 30.024815459074073 30.088795208951137 20.311889148761985 90-91 19.521580178769966 30.194805609435754 29.493834727218253 20.789779484576027 92-93 18.92349873001767 30.638727083764174 29.705726622904432 20.732047563313724 94-95 19.059842128985494 30.046448276783916 29.817637983036622 21.07607161119397 96-97 19.402248415891126 30.694169309508773 29.362745628082575 20.540836646517523 98-99 20.156041145550425 30.51488129191988 28.747797051156095 20.5812805113736 100-101 19.86397669125396 30.994546569389104 27.97507567492738 21.166401064429557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12100.0 1 6803.0 2 1599.0 3 1747.5 4 1478.5 5 1040.5 6 894.0 7 884.0 8 898.5 9 877.0 10 857.5 11 798.0 12 740.0 13 736.0 14 755.0 15 775.0 16 781.0 17 883.5 18 1036.0 19 1156.5 20 1436.5 21 1980.0 22 2748.0 23 3779.5 24 5362.0 25 7393.0 26 9989.0 27 13655.0 28 18261.5 29 23940.5 30 31496.5 31 40772.0 32 57584.0 33 91857.5 34 139104.5 35 182221.0 36 238660.5 37 323049.5 38 402004.0 39 443350.5 40 445085.5 41 433567.5 42 434553.5 43 428868.5 44 380828.5 45 315804.0 46 271880.0 47 236470.0 48 200219.0 49 161104.5 50 118141.0 51 88616.5 52 71793.0 53 54977.5 54 39566.5 55 28972.5 56 21849.5 57 16752.0 58 12682.0 59 9516.0 60 7121.5 61 5613.5 62 4476.0 63 3437.5 64 2586.5 65 1946.5 66 1468.0 67 1183.0 68 999.5 69 852.0 70 702.5 71 550.0 72 422.5 73 325.0 74 251.0 75 179.5 76 127.0 77 95.5 78 71.5 79 51.5 80 38.5 81 28.0 82 19.0 83 11.5 84 10.5 85 10.0 86 9.0 87 7.5 88 2.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15521129235364747 2 0.0 3 0.0051589536620398405 4 0.007133998628365323 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.810499883880977E-5 14-15 0.0 16-17 8.513124854851221E-6 18-19 0.0 20-21 0.0 22-23 1.1067062311306589E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.194174843239318E-4 40-41 0.0 42-43 0.0 44-45 1.191837479679171E-4 46-47 9.534699837433368E-4 48-49 0.002792304952391201 50-51 4.0862999303285856E-4 52-53 0.0013961524761956004 54-55 0.0027837918275363496 56-57 0.00487802054182975 58-59 0.006214581144041392 60-61 0.0017196512206799465 62-63 9.194174843239318E-4 64-65 0.0015493887235829222 66-67 0.00871743985136765 68-69 0.008002337363560147 70-71 0.009781580458224054 72-73 0.006963736131268299 74-75 0.00968793608482069 76-77 0.005431373657395079 78-79 0.004920586166104006 80-81 0.002051663090019144 82-83 0.0 84-85 4.682218670168172E-4 86-87 0.0016089805975668808 88-89 0.005652714903621211 90-91 0.007312774250317199 92-93 0.00961131796112703 94-95 0.008768518600496758 96-97 0.011245837933258463 98-99 0.008070442362398957 100-101 0.007951258614431042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5873284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.779568474757724 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4291625822372 28.777207299785772 2 8.997726450927916 6.4386953933622815 3 3.052441121235426 3.2764507833722747 4 1.993261371956252 2.85272126983993 5 1.0027529080865596 1.7939033169072744 6 0.7648853802707036 1.6420361303242041 7 0.5380214626009571 1.3475123033414174 8 0.32557988019004164 0.9319286093810407 9 0.2691839775437025 0.8668157901149294 >10 2.1577566095532608 14.727271834313116 >50 0.22791993838175337 5.751206146036366 >100 0.2009588502519621 15.116750648684615 >500 0.02553553739999337 6.29902661539474 >1k 0.014530537516489894 8.743861418900076 >5k 1.4169791910172537E-4 0.37214543276108775 >10k+ 1.4169392841218665E-4 1.0624670074807723 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 27835 0.47392566066956743 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19322 0.3289811968908706 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15714 0.2675504879382642 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8438 0.14366749505046922 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7443 0.12672637658931526 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6140 0.104541173217573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.342862357754196E-4 0.0 0.0 2 9.023912346142294E-4 0.0 0.0 3 0.001038601232291849 0.0 0.0 4 0.0010896799814209563 0.0 0.0 5 0.0012429162288082783 0.0 0.0 6 0.0015153362241635173 0.0 0.0 7 0.0017366774703896492 0.0 0.0 8 0.002077202464583698 0.0 0.0 9 0.002383674959358342 0.0 0.0 10-11 0.00327755306911772 0.0 8.513124854851221E-6 12-13 0.005414347407685377 0.0 1.7026249709702442E-5 14-15 0.009066477970416549 0.0 1.7026249709702442E-5 16-17 0.016890039712024824 0.0 1.7026249709702442E-5 18-19 0.026671620170248878 0.0 1.7026249709702442E-5 20-21 0.03336293630616194 0.0 1.7026249709702442E-5 22-23 0.041501483667399704 0.0 1.7026249709702442E-5 24-25 0.05101915725512337 0.0 1.7026249709702442E-5 26-27 0.062043653942155705 0.0 1.7026249709702442E-5 28-29 0.07398756811351195 0.0 1.7026249709702442E-5 30-31 0.08768518600496758 0.0 1.7026249709702442E-5 32-33 0.10191061763742397 0.0 1.7026249709702442E-5 34-35 0.11691925675652667 0.0 1.7026249709702442E-5 36-37 0.132293960244388 0.0 1.7026249709702442E-5 38-39 0.1498650499448009 0.0 1.7026249709702442E-5 40-41 0.1680150321353437 0.0 1.7026249709702442E-5 42-43 0.18728023368187202 0.0 1.7026249709702442E-5 44-45 0.2080011795785799 0.0 1.7026249709702442E-5 46-47 0.2290115717203527 0.0 1.7026249709702442E-5 48-49 0.2514436557128857 0.0 2.5539374564553663E-5 50-51 0.27454827656895187 0.0 3.4052499419404884E-5 52-53 0.2994151142699723 0.0 3.4052499419404884E-5 54-55 0.32646131193383465 0.0 3.4052499419404884E-5 56-57 0.35365223272022944 0.0 3.4052499419404884E-5 58-59 0.3844271790705166 0.0 3.4052499419404884E-5 60-61 0.4181561797454372 0.0 3.4052499419404884E-5 62-63 0.4538176597624089 0.0 3.4052499419404884E-5 64-65 0.4937612415813708 0.0 3.4052499419404884E-5 66-67 0.5373824933376286 0.0 3.4052499419404884E-5 68-69 0.5860435150079581 0.0 3.4052499419404884E-5 70-71 0.6391909534767942 0.0 3.4052499419404884E-5 72-73 0.7015921586628537 0.0 3.4052499419404884E-5 74-75 0.7761415930167859 0.0 3.4052499419404884E-5 76-77 0.8650782083754165 0.0 3.4052499419404884E-5 78-79 0.9735864977753502 0.0 3.4052499419404884E-5 80-81 1.1083492642276451 0.0 3.4052499419404884E-5 82-83 1.2786032481998146 0.0 3.4052499419404884E-5 84-85 1.4912611070739983 0.0 3.4052499419404884E-5 86-87 1.7338170604384193 0.0 3.4052499419404884E-5 88-89 2.02045397430126 0.0 3.4052499419404884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTGG 285 0.0 56.76117 1 CCGGACC 65 4.3659384E-8 51.23916 1 GGCACGC 105 0.0 49.844894 1 GTTACGG 270 0.0 49.34141 1 CGCGGAG 420 0.0 46.44638 1 TGTACCG 180 0.0 44.86438 2 TCAGCCG 350 0.0 44.78898 2 ATGACCG 150 0.0 44.407272 1 CGAGGGG 1145 0.0 43.216057 1 CGGGTGG 670 0.0 41.18798 1 CGACGGG 285 0.0 40.066708 1 ACGGGGC 300 0.0 39.64935 1 GCGGCCC 120 8.731149E-11 39.58622 2 GTTGACG 265 0.0 39.499725 1 CGTACCG 110 1.6207196E-9 38.86647 2 CGGAGCG 100 2.9902367E-8 38.063374 1 CGAGCGG 190 0.0 37.562542 1 GGTACGG 115 2.4665496E-9 37.23591 1 GGACGGG 710 0.0 36.85714 1 CGTCCCG 130 2.055458E-10 36.541126 3 >>END_MODULE