FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873284
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT278350.47392566066956743TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC193220.3289811968908706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157140.2675504879382642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84380.14366749505046922No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG74430.12672637658931526No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA61400.104541173217573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG2850.056.761171
CCGGACC654.3659384E-851.239161
GGCACGC1050.049.8448941
GTTACGG2700.049.341411
CGCGGAG4200.046.446381
TGTACCG1800.044.864382
TCAGCCG3500.044.788982
ATGACCG1500.044.4072721
CGAGGGG11450.043.2160571
CGGGTGG6700.041.187981
CGACGGG2850.040.0667081
ACGGGGC3000.039.649351
GCGGCCC1208.731149E-1139.586222
GTTGACG2650.039.4997251
CGTACCG1101.6207196E-938.866472
CGGAGCG1002.9902367E-838.0633741
CGAGCGG1900.037.5625421
GGTACGG1152.4665496E-937.235911
GGACGGG7100.036.857141
CGTCCCG1302.055458E-1036.5411263
Produced by FastQC (version 0.11.2)