##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3820979 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67648081813588 33.0 33.0 33.0 33.0 33.0 2 32.71694139119843 33.0 33.0 33.0 33.0 33.0 3 32.74306375407978 33.0 33.0 33.0 33.0 33.0 4 36.685685527190806 37.0 37.0 37.0 37.0 37.0 5 36.681438186391496 37.0 37.0 37.0 37.0 37.0 6 36.562657109604636 37.0 37.0 37.0 37.0 37.0 7 36.51482826783398 37.0 37.0 37.0 37.0 37.0 8 36.46190832244825 37.0 37.0 37.0 37.0 37.0 9 36.46243227193868 37.0 37.0 37.0 37.0 37.0 10-11 36.41893399571157 37.0 37.0 37.0 37.0 37.0 12-13 36.42139763134003 37.0 37.0 37.0 37.0 37.0 14-15 38.33097394672936 40.0 37.0 40.0 37.0 40.0 16-17 38.29439902705563 40.0 37.0 40.0 37.0 40.0 18-19 38.29032559456621 40.0 37.0 40.0 37.0 40.0 20-21 38.22819701443007 40.0 37.0 40.0 37.0 40.0 22-23 38.147576053152875 40.0 37.0 40.0 37.0 40.0 24-25 38.110943556612064 40.0 37.0 40.0 37.0 40.0 26-27 38.02771606439083 40.0 37.0 40.0 37.0 40.0 28-29 37.91626033537478 40.0 37.0 40.0 33.0 40.0 30-31 37.885551451604414 40.0 37.0 40.0 33.0 40.0 32-33 37.758665645636896 40.0 37.0 40.0 33.0 40.0 34-35 37.68768135077424 40.0 37.0 40.0 33.0 40.0 36-37 37.60529277967767 40.0 37.0 40.0 33.0 40.0 38-39 37.513043515811 40.0 37.0 40.0 33.0 40.0 40-41 37.45336718678642 40.0 37.0 40.0 33.0 40.0 42-43 37.33209839677214 40.0 37.0 40.0 33.0 40.0 44-45 37.20039013561708 40.0 37.0 40.0 33.0 40.0 46-47 37.12750580414077 40.0 37.0 40.0 33.0 40.0 48-49 36.94321088914647 37.0 37.0 40.0 33.0 40.0 50-51 36.792636782353426 37.0 37.0 40.0 33.0 40.0 52-53 36.63654432542026 37.0 37.0 40.0 33.0 40.0 54-55 36.46995678332699 37.0 37.0 40.0 33.0 40.0 56-57 36.29885848626752 37.0 37.0 40.0 33.0 40.0 58-59 36.06792473865991 37.0 37.0 40.0 33.0 40.0 60-61 35.78532321166905 37.0 37.0 40.0 30.0 40.0 62-63 35.508292900850805 37.0 33.0 40.0 27.0 40.0 64-65 35.232580838575664 37.0 33.0 37.0 27.0 40.0 66-67 34.957223135746105 37.0 33.0 37.0 27.0 40.0 68-69 34.67671570558226 37.0 33.0 37.0 27.0 40.0 70-71 34.309137658176084 37.0 33.0 37.0 27.0 40.0 72-73 34.01830695745776 37.0 33.0 37.0 27.0 37.0 74-75 33.67744156667702 37.0 33.0 37.0 27.0 37.0 76-77 30.924316516787975 33.0 30.0 35.0 24.5 37.0 78-79 33.42619169066357 35.0 33.0 37.0 27.0 37.0 80-81 33.84617947389923 37.0 33.0 37.0 27.0 37.0 82-83 33.80154758767321 37.0 33.0 37.0 27.0 37.0 84-85 33.63670789606538 37.0 33.0 37.0 27.0 37.0 86-87 33.48039468418958 37.0 33.0 37.0 27.0 37.0 88-89 33.34496172316048 37.0 33.0 37.0 27.0 37.0 90-91 33.19589626114145 37.0 33.0 37.0 27.0 37.0 92-93 33.08137404052731 37.0 33.0 37.0 27.0 37.0 94-95 32.90868282709745 33.0 33.0 37.0 27.0 37.0 96-97 32.752879955634405 33.0 33.0 37.0 27.0 37.0 98-99 32.620999356447655 33.0 33.0 37.0 27.0 37.0 100-101 30.97239254651753 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1310830246408088 2306 2 0.13300079182762659 2306 3 0.10571112811666694 2306 4 0.13919631849914538 2306 5 0.1287337741649921 2306 6 0.1656227073973895 2306 7 0.2319213439848653 2306 8 0.2330181336363708 2306 9 0.22104296811554747 2306 10-11 0.21946631588070176 2306 12-13 0.23736106378515132 2306 14-15 0.48028582655717145 2306 16-17 0.3841530649778164 2306 18-19 0.38038332772770644 2306 20-21 0.40060926874522096 2306 22-23 0.4243867322478394 2306 24-25 0.5186640754986414 2306 26-27 0.5805213954883897 2306 28-29 0.5351085817168126 2306 30-31 0.5514278679117268 2306 32-33 0.5803968169025566 2306 34-35 0.5623414923264178 2306 36-37 0.5299067429552764 2306 38-39 0.5932986761127594 2306 40-41 0.552137263699926 2306 42-43 0.5644213626662378 2306 44-45 0.6013217697013289 2306 46-47 0.5360628572726256 2306 48-49 0.5647382872472733 2306 50-51 0.6415731516507748 2306 52-53 0.6913773424304992 2306 54-55 0.7030340244825553 2306 56-57 0.5741782602110348 2306 58-59 0.6126945903335326 2306 60-61 0.6284567705411988 2306 62-63 0.5546444551967227 2306 64-65 0.5973637887527303 2306 66-67 0.6792222904480312 2306 68-69 0.6221326610868445 2306 70-71 0.5924020713551457 2306 72-73 0.5141927560409769 2306 74-75 0.5632916383800435 2306 76-77 0.08526899563526413 2306 78-79 0.38098507026627715 2306 80-81 0.5511716874584778 2306 82-83 0.6392039217207355 2306 84-85 0.6628066956683867 2306 86-87 0.547121425249415 2306 88-89 0.6304250779512159 2306 90-91 0.4679687688222316 2306 92-93 0.5416537175255911 2306 94-95 0.5128682672883187 2306 96-97 0.6271736628742417 2306 98-99 0.5693191209815609 2306 100-101 0.43694645829531353 2307 1 0.1420259886520725 2307 2 0.11550252136525785 2307 3 0.11930870992163989 2307 4 0.12085925816770526 2307 5 0.13126610431865515 2307 6 0.1646039882025363 2307 7 0.16863915520834638 2307 8 0.18105886173583485 2307 9 0.19967397807941012 2307 10-11 0.21627989038287154 2307 12-13 0.21999828573147795 2307 14-15 0.3635945971070882 2307 16-17 0.39598941770589846 2307 18-19 0.32599604334578913 2307 20-21 0.2988098606633329 2307 22-23 0.3150612375713706 2307 24-25 0.3798961952371869 2307 26-27 0.4595374105762744 2307 28-29 0.3634042192631384 2307 30-31 0.4176451896975806 2307 32-33 0.43179930686262225 2307 34-35 0.3514348127228999 2307 36-37 0.4136818714864745 2307 38-39 0.37976241036392366 2307 40-41 0.3602886247958068 2307 42-43 0.3021365932186342 2307 44-45 0.3207762405202317 2307 46-47 0.2514420494278369 2307 48-49 0.36836816332338884 2307 50-51 0.3171807863035312 2307 52-53 0.4283923183605012 2307 54-55 0.3294325960710651 2307 56-57 0.34328742632988707 2307 58-59 0.3498838692096342 2307 60-61 0.31663345034564827 2307 62-63 0.2661454068012432 2307 64-65 0.2449140458151291 2307 66-67 0.2684878469572638 2307 68-69 0.19423161647482345 2307 70-71 0.22594080582160103 2307 72-73 0.1389102457570459 2307 74-75 0.14350802434969268 2307 76-77 -0.26371133787126766 2307 78-79 -0.009437223976149767 2307 80-81 0.20237932096300426 2307 82-83 0.20807528717993762 2307 84-85 0.2942659661336293 2307 86-87 0.16068306711886748 2307 88-89 0.15641137369784275 2307 90-91 0.17655904494542796 2307 92-93 0.17343788271150373 2307 94-95 0.18904927596040721 2307 96-97 0.2655544711783122 2307 98-99 0.0803146170072111 2307 100-101 -0.025365065161810918 2201 1 0.12442347498798512 2201 2 0.12270712622888169 2201 3 0.11421325933679327 2201 4 0.10994805493585602 2201 5 0.07956911701899827 2201 6 0.07887167230467185 2201 7 0.11170255129526652 2201 8 0.10464188922243522 2201 9 0.1119507979774923 2201 10-11 0.20221419714494715 2201 12-13 0.1266272826422039 2201 14-15 0.28838085644751743 2201 16-17 0.22121715250377605 2201 18-19 0.16948480842909674 2201 20-21 0.18702389176728929 2201 22-23 0.2423653856451935 2201 24-25 0.26703822253895737 2201 26-27 0.2664888007486894 2201 28-29 0.2855247272767585 2201 30-31 0.29694442662098197 2201 32-33 0.330439763124339 2201 34-35 0.241692516099981 2201 36-37 0.18547998623841266 2201 38-39 0.23181294768603067 2201 40-41 0.19260320246326756 2201 42-43 0.23180337430278541 2201 44-45 0.22470964954512596 2201 46-47 0.23897115987591633 2201 48-49 0.34424411716913283 2201 50-51 0.31351062550296405 2201 52-53 0.17253854390376233 2201 54-55 0.23341670084226962 2201 56-57 0.27785426814332226 2201 58-59 0.2283635249010203 2201 60-61 0.15122986467775945 2201 62-63 0.25073812456852096 2201 64-65 0.2877435021560615 2201 66-67 0.24295725788325484 2201 68-69 0.2769926863663059 2201 70-71 0.3084774414195195 2201 72-73 0.2014555946863652 2201 74-75 0.1630417290997812 2201 76-77 0.032067449127389125 2201 78-79 0.2884911154741161 2201 80-81 0.3229749959821433 2201 82-83 0.3069209027899973 2201 84-85 0.3540344027507487 2201 86-87 0.3075267288303749 2201 88-89 0.13954526855697225 2201 90-91 0.19153409313278758 2201 92-93 0.12643078002621877 2201 94-95 0.20326573623553656 2201 96-97 0.1610921893416517 2201 98-99 0.06094699363436007 2201 100-101 0.09856385517721833 1101 1 0.12075769988792473 1101 2 0.09349233890439734 1101 3 0.07380064127539043 1101 4 0.09683969659595704 1101 5 0.09124501638196136 1101 6 0.09406544246682103 1101 7 0.16660105633604871 1101 8 0.07611635146801632 1101 9 0.15046336984306663 1101 10-11 0.08177390040466292 1101 12-13 0.08820380273738948 1101 14-15 0.22003625883650813 1101 16-17 0.19318773468771155 1101 18-19 0.11354738242977191 1101 20-21 0.14023741355890706 1101 22-23 0.23536254401925305 1101 24-25 0.17827021489917882 1101 26-27 0.19557237638368008 1101 28-29 0.15702607239067845 1101 30-31 0.2722744938960915 1101 32-33 0.27733629610776944 1101 34-35 0.23413823599795336 1101 36-37 0.1594317484735086 1101 38-39 0.13630830675186445 1101 40-41 0.13458017054156102 1101 42-43 0.18467673561241327 1101 44-45 0.10782041472667458 1101 46-47 0.29769256407391254 1101 48-49 0.22585613817853556 1101 50-51 0.21620095038776554 1101 52-53 0.2335764361316066 1101 54-55 0.17113414151556583 1101 56-57 0.21961345587308756 1101 58-59 0.11157522215140148 1101 60-61 0.1346773935889729 1101 62-63 0.21100301301917312 1101 64-65 0.21926313150244425 1101 66-67 0.28147555570273397 1101 68-69 0.2388831634256121 1101 70-71 0.145739717282396 1101 72-73 0.07384999450934515 1101 74-75 0.07623675314878398 1101 76-77 0.002736298773545087 1101 78-79 0.05594984008096304 1101 80-81 0.2838090830709916 1101 82-83 0.3607304090380836 1101 84-85 0.4012195626310202 1101 86-87 0.30900140458610537 1101 88-89 0.19929550050663636 1101 90-91 0.11955157379071579 1101 92-93 0.1564593321556913 1101 94-95 0.256231877408446 1101 96-97 0.20103392048191182 1101 98-99 0.10824172303936308 1101 100-101 0.14299007577589862 2304 1 0.03203311774139195 2304 2 0.06726129410573378 2304 3 0.07224404644106386 2304 4 0.13597187137500555 2304 5 0.10379217830276133 2304 6 0.0499143676285172 2304 7 0.10171126462666535 2304 8 0.10455475590845964 2304 9 0.10007162283952908 2304 10-11 0.11192956165263723 2304 12-13 0.10042919957511032 2304 14-15 0.2117761512485643 2304 16-17 0.0998259239240511 2304 18-19 0.027065406744519294 2304 20-21 0.04817695595549054 2304 22-23 0.10154342805007133 2304 24-25 0.07030572181285066 2304 26-27 0.05671534736500661 2304 28-29 0.10421482312340657 2304 30-31 0.19038038363521537 2304 32-33 0.13452050666194992 2304 34-35 0.153266724639046 2304 36-37 0.040185185192811446 2304 38-39 0.08221956981788736 2304 40-41 0.11655034158612665 2304 42-43 0.1333282072972679 2304 44-45 0.16201723014344793 2304 46-47 0.13459997195840856 2304 48-49 0.1829748752289717 2304 50-51 0.05791957118986346 2304 52-53 0.1000291044614201 2304 54-55 0.06151719459771954 2304 56-57 0.14292833146019035 2304 58-59 0.12057961551966656 2304 60-61 0.15455545282762984 2304 62-63 0.1437965038888791 2304 64-65 -0.01800729656598321 2304 66-67 0.07298920676503684 2304 68-69 0.1557466799765237 2304 70-71 0.048776599130818 2304 72-73 0.07514133386730748 2304 74-75 0.04395953334026359 2304 76-77 -0.42417710504048856 2304 78-79 0.10121257931378835 2304 80-81 0.2376714816051475 2304 82-83 0.28101326410281047 2304 84-85 0.3318008854692991 2304 86-87 0.26679190454922974 2304 88-89 0.30479359850178867 2304 90-91 0.15864126710897608 2304 92-93 0.14978250817028282 2304 94-95 0.2623130786694574 2304 96-97 0.17210003133991592 2304 98-99 0.2714175135298049 2304 100-101 0.1960950779480548 2202 1 0.09619765210720033 2202 2 0.09365437027245349 2202 3 0.07088651891105258 2202 4 0.08023586059595544 2202 5 0.04527105795563813 2202 6 0.05031528877282199 2202 7 0.09895683410177725 2202 8 0.026629664147122867 2202 9 0.05420073619666255 2202 10-11 0.023773492126032636 2202 12-13 0.05015599101986368 2202 14-15 0.0446727578001358 2202 16-17 -0.03088463573013911 2202 18-19 -0.01972657589549698 2202 20-21 0.019161747604933055 2202 22-23 0.018836632980210766 2202 24-25 -0.06978195583270264 2202 26-27 -0.02568558328094639 2202 28-29 0.004883207548083135 2202 30-31 -0.07069428794804367 2202 32-33 -0.06619302668531901 2202 34-35 -0.09841838823849258 2202 36-37 -0.11315680537462924 2202 38-39 0.027198960506318315 2202 40-41 0.010524735938737706 2202 42-43 -0.10723595373437433 2202 44-45 -0.11147101974275131 2202 46-47 -0.060408507455349536 2202 48-49 -0.1732659869858182 2202 50-51 -0.011316144595291178 2202 52-53 -0.017490916029935022 2202 54-55 -0.013153422391660285 2202 56-57 -0.00743396710390698 2202 58-59 -0.046730519249905456 2202 60-61 -0.04506297556631722 2202 62-63 -0.2745263815258596 2202 64-65 -0.1116909486057267 2202 66-67 -0.08443056008872674 2202 68-69 -0.0925387359390939 2202 70-71 -0.005970193681200442 2202 72-73 -0.25556647192001947 2202 74-75 -0.1858614065319486 2202 76-77 -0.5904348117409448 2202 78-79 -0.11373405726862984 2202 80-81 0.00636051455580855 2202 82-83 0.04192640770544642 2202 84-85 0.01822406413865707 2202 86-87 -0.02669537745745032 2202 88-89 0.05114434242027244 2202 90-91 0.061213080830654576 2202 92-93 -0.04746316598397726 2202 94-95 -0.037688266055006636 2202 96-97 -0.02909550923167359 2202 98-99 -0.134524848036655 2202 100-101 -0.01596176991634124 1102 1 0.04314579249235351 1102 2 0.10004779950808995 1102 3 0.058440809024631335 1102 4 0.018613123590149883 1102 5 0.06409436754665165 1102 6 0.019875692340065143 1102 7 0.0941995422356996 1102 8 -0.003913046146720944 1102 9 -0.03587432536838264 1102 10-11 0.012242122581639592 1102 12-13 -0.010734667160200217 1102 14-15 -0.004506340472843817 1102 16-17 0.010580340027004809 1102 18-19 -0.009075002809957766 1102 20-21 -0.06777048632061877 1102 22-23 -0.01170093641454173 1102 24-25 -0.07457212726208695 1102 26-27 -0.10326452456048685 1102 28-29 -0.17471391757238308 1102 30-31 -0.008808588405557316 1102 32-33 -0.011328796720995626 1102 34-35 -0.03166784478258933 1102 36-37 0.03279280227491199 1102 38-39 0.035912599555736335 1102 40-41 0.014743231991268146 1102 42-43 -0.0648092599967498 1102 44-45 -0.08182256699804924 1102 46-47 -0.09998473907423744 1102 48-49 -0.09700378160371059 1102 50-51 -0.1295985428373001 1102 52-53 -0.07610286143786738 1102 54-55 -0.07600779307312422 1102 56-57 -0.21162715070072124 1102 58-59 -0.24666622472476263 1102 60-61 -0.18645685897634934 1102 62-63 -0.1574570875318244 1102 64-65 -0.06788863903276621 1102 66-67 -0.15190389916408975 1102 68-69 -0.013142542305175198 1102 70-71 -0.031832022882632316 1102 72-73 -0.17322321164613186 1102 74-75 -0.3248380687732535 1102 76-77 -0.40946669389585466 1102 78-79 -0.1397636129262949 1102 80-81 -0.004085570505282021 1102 82-83 0.03683129589422407 1102 84-85 -0.026673774730916477 1102 86-87 -0.040505533743747435 1102 88-89 -0.06244522861126711 1102 90-91 -0.044750391712796045 1102 92-93 -0.06280138980408623 1102 94-95 -0.05640809193607765 1102 96-97 -0.08253619094892173 1102 98-99 -0.14877959963284582 1102 100-101 -0.2342322516281996 2305 1 0.023078746309778353 2305 2 0.019004695173236996 2305 3 0.007399772408376748 2305 4 0.026526624571502566 2305 5 -0.019182252770015396 2305 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-0.33404893795640334 1213 88-89 -0.6571834961625882 1213 90-91 -0.420435228227646 1213 92-93 -0.2558872433129764 1213 94-95 -0.1526728872027192 1213 96-97 -0.23143808737591343 1213 98-99 -0.2927013975327526 1213 100-101 -0.08561608536001941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 8.0 8 51.0 9 172.0 10 277.0 11 208.0 12 152.0 13 196.0 14 420.0 15 857.0 16 1699.0 17 2678.0 18 3773.0 19 4948.0 20 6209.0 21 8241.0 22 10261.0 23 12827.0 24 16364.0 25 20463.0 26 25844.0 27 32940.0 28 43028.0 29 57035.0 30 68546.0 31 84741.0 32 114094.0 33 162035.0 34 247787.0 35 427731.0 36 830680.0 37 1294125.0 38 341683.0 39 904.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.896773784304195 32.28750388591733 15.845578232362392 18.97014409741608 2 30.772218324152007 24.630101343137454 29.93790334885379 14.659776983856753 3 32.635677844177536 22.968898897271956 24.240282851980883 20.155140406569625 4 28.39326740820656 22.84221364787272 28.67366120012626 20.09085774379446 5 31.41074578007364 21.06394722399678 27.919467759440707 19.60583923648887 6 28.46006219871923 21.48431069629014 30.202966307849376 19.852660797141255 7 31.242909212534276 22.32543021042513 24.590399476155195 21.8412611008854 8 27.601224712305406 25.268340914723687 25.327697430422937 21.80273694254797 9 26.721214641587927 22.66898090777259 28.346112344506473 22.26369210613301 10-11 21.998315091498803 29.281252265453432 31.06690719839078 17.653525444656985 12-13 22.074809391052423 28.610924822247707 30.1510542786499 19.16321150804997 14-15 22.080584583165727 28.450169446102684 30.41114593929985 19.058100031431735 16-17 22.36699023993589 27.83031521502735 30.659249893809935 19.14344465122682 18-19 21.588472483099228 27.886949915191895 30.995433369301427 19.529144232407454 20-21 21.947084765448853 28.10974621948982 30.56962626593865 19.37354274912267 22-23 21.54633972014709 28.906509771140666 30.005497293031368 19.54165321568088 24-25 21.336887745261095 28.183248324578596 30.538560929018455 19.941303001141854 26-27 21.298298158665617 28.279676491286658 30.51817348381135 19.903851866236373 28-29 21.348154491296604 29.174931346128837 30.056145296794355 19.42076886578021 30-31 20.727842262414946 29.474553511024272 30.259443980194607 19.53816024636618 32-33 20.579895885321537 29.800713377383126 29.99192091869649 19.627469818598847 34-35 21.036650554740028 29.448342950851075 30.284175861735957 19.230830632672934 36-37 20.637538180659984 28.95774878637124 31.15437169374655 19.250341339222228 38-39 20.92740541953098 29.075571596985505 30.455978169766883 19.541044813716628 40-41 20.80058539971039 29.08942969851444 30.37370789004598 19.736277011729193 42-43 21.072636096665278 29.12555918260739 30.032800494323574 19.76900422640376 44-45 20.64654735848696 28.846579130498963 30.391460499720885 20.115413011293196 46-47 21.03505253227841 29.023211979576214 30.033605635686435 19.908129852458945 48-49 20.669380364954296 29.750721172342786 29.932486438923995 19.647412023778926 50-51 21.18783730061626 29.353126085296346 29.333654543974667 20.125382070112728 52-53 20.72718183995195 29.072578165177713 29.34311545794659 20.857124536923745 54-55 21.24114403264178 29.169011246129806 29.540602009489987 20.049242711738426 56-57 20.969580985266244 28.865093019509473 29.70057588958498 20.4647501056393 58-59 21.03944935195052 29.33378385752792 29.605825790826344 20.020940999695217 60-61 21.033966994816687 29.528646934045906 29.318749566530528 20.118636504606883 62-63 20.584102112005638 29.502296750683044 29.239037027915764 20.67456410939555 64-65 20.864606358995406 29.254134739347982 29.321160516922895 20.56009838473372 66-67 21.01003195405798 29.47082315888575 29.08978767047366 20.42935721658261 68-69 21.04512324935764 29.665354418296904 29.1459951123743 20.143527219971162 70-71 20.98434170184008 30.165400982150942 28.965587549998773 19.88466976601021 72-73 20.652792111206526 30.15098498682575 28.9326515405117 20.263571361456023 74-75 20.84199797780612 29.564652490719396 28.873151698828693 20.72019783264579 76-77 20.896031779993997 29.213298319434948 28.998642550575887 20.892027349995164 78-79 20.59758544856859 28.862114338844624 29.702385041846686 20.8379151707401 80-81 20.523793234939337 29.27804649013046 29.415252491757826 20.78290778317238 82-83 20.111900641170756 29.539288229534893 29.272118480630226 21.076692648664125 84-85 20.149234688703356 29.54565231814034 29.354012072615753 20.95110092054055 86-87 20.271206969945325 29.344036167210536 29.842764287496763 20.541992575347372 88-89 20.420999955375375 29.803425260494603 29.533779989612015 20.241794794518004 90-91 20.714595119474335 29.611680597378225 28.945689200525038 20.728035082622405 92-93 20.350289246702232 29.73048311082983 29.01511380108014 20.904113841387797 94-95 19.940674907338895 29.864842122124468 28.9899108605541 21.204572109982532 96-97 20.037738086217313 30.235305526556626 28.858996108799705 20.867960278426352 98-99 20.304433475747256 30.019835523633876 28.759755650218988 20.915975350399883 100-101 20.42128709016541 30.00399798726533 28.337314962221967 21.237399960347293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 212.0 1 132.0 2 75.5 3 139.5 4 146.0 5 86.5 6 64.0 7 69.0 8 77.5 9 80.0 10 74.5 11 77.0 12 76.5 13 81.0 14 89.0 15 108.5 16 146.5 17 162.5 18 195.5 19 271.5 20 434.5 21 726.5 22 1141.0 23 1786.5 24 2759.5 25 4385.5 26 7257.0 27 11530.0 28 16260.5 29 21511.5 30 27426.5 31 31811.0 32 38900.0 33 54358.0 34 76546.5 35 108750.0 36 151395.0 37 202474.0 38 253746.0 39 272324.5 40 277984.0 41 266952.5 42 233973.0 43 219995.0 44 207681.0 45 197820.5 46 201907.5 47 188393.5 48 154835.5 49 126183.5 50 101138.0 51 82000.5 52 67722.0 53 54175.0 54 44076.5 55 34030.5 56 23450.5 57 15156.0 58 9941.0 59 7111.5 60 5330.5 61 3943.5 62 2767.0 63 1837.5 64 1299.5 65 943.5 66 666.0 67 503.0 68 390.5 69 268.5 70 187.5 71 152.5 72 101.5 73 64.0 74 51.5 75 45.5 76 36.5 77 23.0 78 15.5 79 12.0 80 6.5 81 5.0 82 6.0 83 5.5 84 3.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1549602863559313 2 0.0 3 0.0047631771857421885 4 0.007354136204360192 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.9256954827545505E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.9628477413772752E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001033766477125365 40-41 0.0 42-43 0.0 44-45 1.5702781931018202E-4 46-47 0.001112280386780456 48-49 0.0030620424765485494 50-51 4.7108345793054604E-4 52-53 0.001674963405975275 54-55 0.00285267205080164 56-57 0.005090318475971734 58-59 0.006398883636889917 60-61 0.001674963405975275 62-63 9.814238706886377E-4 64-65 0.001596449496320184 66-67 0.008832814836197739 68-69 0.008113103997692737 70-71 0.010075951739070013 72-73 0.007013909262521464 74-75 0.00984041001010474 76-77 0.005509059327465553 78-79 0.004880948050224825 80-81 0.002146046863905821 82-83 0.0 84-85 4.579978063213642E-4 86-87 0.0015571925414926385 88-89 0.005718429753212463 90-91 0.007432650114015282 92-93 0.009552525674702739 94-95 0.008793557881370193 96-97 0.011397602551597379 98-99 0.008073847042865193 100-101 0.008021504436428465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3820979.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.36916293987018 #Duplication Level Percentage of deduplicated Percentage of total 1 87.60428994593123 37.117204349507915 2 5.652846848881106 4.790127784287506 3 1.6281130246358368 2.0694535807596193 4 0.9444034253825957 1.6005433044402693 5 0.6838944753990386 1.4488018230929454 6 0.4495598610843344 1.142848500330454 7 0.3264450847266713 0.9681843489992844 8 0.27298240426489456 0.9252828772813465 9 0.2487258606447598 0.9484475865315546 >10 1.767049541702308 15.225491184028275 >50 0.22283499373920607 6.6364287179226 >100 0.1650903086774857 13.708676361406422 >500 0.023497682556559943 6.942769036994393 >1k 0.010204703749735141 5.998576903760939 >5k 0.0 0.0 >10k+ 6.183862407434147E-5 0.4771636406564597 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 18212 0.47663177421283914 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.159956126427285E-4 0.0 0.0 2 0.0010206808255161831 0.0 0.0 3 0.0014655929802283656 0.0 0.0 4 0.0018058199220670932 0.0 0.0 5 0.002146046863905821 0.0 0.0 6 0.003088213779766913 0.0 0.0 7 0.004056551998846369 0.0 0.0 8 0.005234260643672734 0.0 0.0 9 0.005757686708040007 0.0 0.0 10-11 0.007746705752635647 0.0 0.0 12-13 0.010756405622747468 0.0 0.0 14-15 0.01596449496320184 0.0 0.0 16-17 0.025687134108823943 0.0 0.0 18-19 0.0375296488151335 0.0 0.0 20-21 0.04748782968972089 0.0 0.0 22-23 0.05976217089913344 0.0 0.0 24-25 0.0736983898629121 0.0 0.0 26-27 0.08900860224565485 0.0 0.0 28-29 0.1056142941377066 0.0 0.0 30-31 0.12669527888009852 0.0 0.0 32-33 0.14576107327467647 0.0 1.3085651609181835E-5 34-35 0.1681375375263774 0.0 2.617130321836367E-5 36-37 0.19335358817727077 0.0 3.9256954827545505E-5 38-39 0.22083345655655265 0.0 5.234260643672734E-5 40-41 0.2521343352057156 0.0 5.234260643672734E-5 42-43 0.28501857769958955 0.0 5.234260643672734E-5 44-45 0.3215144600375977 0.0 5.234260643672734E-5 46-47 0.3622500934969808 0.0 5.234260643672734E-5 48-49 0.4070684502584285 0.0 5.234260643672734E-5 50-51 0.45416371039987397 0.0 5.234260643672734E-5 52-53 0.5033919317536161 0.0 5.234260643672734E-5 54-55 0.5558392234032168 0.0 5.234260643672734E-5 56-57 0.6134030048320077 0.0 5.234260643672734E-5 58-59 0.676318817768954 0.0 5.234260643672734E-5 60-61 0.7413283349633693 0.0 5.234260643672734E-5 62-63 0.8092166955118048 0.0 5.234260643672734E-5 64-65 0.8829805136327626 0.0 5.234260643672734E-5 66-67 0.9614813376362445 0.0 5.234260643672734E-5 68-69 1.0448369383867329 0.0 5.234260643672734E-5 70-71 1.1322752624392858 0.0 5.234260643672734E-5 72-73 1.2243459071614893 0.0 5.234260643672734E-5 74-75 1.3248698828232242 0.0 5.234260643672734E-5 76-77 1.4342135876695474 0.0 5.234260643672734E-5 78-79 1.550832914810576 0.0 5.234260643672734E-5 80-81 1.6755784316009064 0.0 5.234260643672734E-5 82-83 1.8096540179885836 0.0 5.234260643672734E-5 84-85 1.9575218811723383 0.0 5.234260643672734E-5 86-87 2.1130971931539015 0.0 5.234260643672734E-5 88-89 2.2787746281777523 0.0 5.234260643672734E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGTG 25 0.0049952418 57.077465 1 CGCGGGT 25 0.0049952418 57.077465 1 CGAGTCG 45 1.5044954E-5 52.849506 1 CGAGGCG 190 0.0 52.571354 1 CGTTGCG 40 5.353792E-4 47.564556 1 TCGCCGG 70 7.92279E-8 47.50355 2 CGAGGTG 200 0.0 45.18633 1 CGAGTGG 170 0.0 44.766644 1 CGACCGC 55 4.925848E-5 43.24051 1 CGGTGCG 55 4.925848E-5 43.24051 1 ACGACGG 55 4.925848E-5 43.24051 1 CGAGCGG 100 6.2937033E-10 42.808098 1 CGGGTCG 70 4.2727697E-6 40.76962 1 CGACCGA 60 8.2282626E-5 39.63713 1 GGCGCGT 60 8.290721E-5 39.586296 3 CGTGCGG 50 0.0016055381 38.051643 1 GGCCCGC 75 6.8622485E-6 38.051643 1 GTGACGC 100 2.9978764E-8 38.051643 1 AGTCGCC 75 6.8622485E-6 38.051643 1 CGACGGG 215 0.0 37.609184 1 >>END_MODULE