FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT182120.47663177421283914TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG250.004995241857.0774651
CGCGGGT250.004995241857.0774651
CGAGTCG451.5044954E-552.8495061
CGAGGCG1900.052.5713541
CGTTGCG405.353792E-447.5645561
TCGCCGG707.92279E-847.503552
CGAGGTG2000.045.186331
CGAGTGG1700.044.7666441
CGACCGC554.925848E-543.240511
CGGTGCG554.925848E-543.240511
ACGACGG554.925848E-543.240511
CGAGCGG1006.2937033E-1042.8080981
CGGGTCG704.2727697E-640.769621
CGACCGA608.2282626E-539.637131
GGCGCGT608.290721E-539.5862963
CGTGCGG500.001605538138.0516431
GGCCCGC756.8622485E-638.0516431
GTGACGC1002.9978764E-838.0516431
AGTCGCC756.8622485E-638.0516431
CGACGGG2150.037.6091841
Produced by FastQC (version 0.11.2)