FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047836
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2414457.921850125794171TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.22202638199693162No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT55680.1826869949695456TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52730.17300799649324963No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC50470.16559289935547714No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG47210.1548967857850619No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG42970.14098527611065687No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34410.11289977544723535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32180.10558310880244212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG335150.079.295883
GATCGGA337200.078.997161
CGGAAGA335900.078.904134
GAAGAGC336750.078.72936
ATCGGAA339150.078.4026642
GGAAGAG346500.076.5962145
GAGCACA415500.063.7962539
AGAGCAC416300.063.719298
AAGAGCA422750.062.6797037
CGAGTCG250.00499035957.0912931
ATCGGAC1400.054.285242
TCGGACG1350.052.7773133
GCCGTCT286400.045.11888550-51
CGTCTTC286850.044.95022652-53
ATGCCGT287250.044.8854248-49
TCGTATG293350.044.71233744-45
TCTCGTA287400.044.6708442-43
AATCTCG287900.044.63340840-41
CTGCTTG288950.044.59888558-59
GTATGCC292450.044.50075546-47
Produced by FastQC (version 0.11.2)