##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3047836 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68467791574087 33.0 33.0 33.0 33.0 33.0 2 32.709671714619816 33.0 33.0 33.0 33.0 33.0 3 32.75106468983239 33.0 33.0 33.0 33.0 33.0 4 36.67932231261787 37.0 37.0 37.0 37.0 37.0 5 36.68193400169825 37.0 37.0 37.0 37.0 37.0 6 36.573224084235505 37.0 37.0 37.0 37.0 37.0 7 36.451903908215534 37.0 37.0 37.0 37.0 37.0 8 36.410529634796625 37.0 37.0 37.0 37.0 37.0 9 36.444385459060136 37.0 37.0 37.0 37.0 37.0 10-11 36.376125388636396 37.0 37.0 37.0 37.0 37.0 12-13 36.37585913415289 37.0 37.0 37.0 37.0 37.0 14-15 38.23231729003791 40.0 37.0 40.0 37.0 40.0 16-17 38.25119330567655 40.0 37.0 40.0 37.0 40.0 18-19 38.21881344665526 40.0 37.0 40.0 37.0 40.0 20-21 38.207701300201194 40.0 37.0 40.0 37.0 40.0 22-23 37.99131547760444 40.0 37.0 40.0 33.0 40.0 24-25 38.05690217583886 40.0 37.0 40.0 37.0 40.0 26-27 37.98318954825653 40.0 37.0 40.0 37.0 40.0 28-29 37.765826803016964 40.0 37.0 40.0 33.0 40.0 30-31 37.74890889798533 40.0 37.0 40.0 33.0 40.0 32-33 37.60955838831223 40.0 37.0 40.0 33.0 40.0 34-35 37.605099815081914 40.0 37.0 40.0 33.0 40.0 36-37 37.50976003958218 40.0 37.0 40.0 33.0 40.0 38-39 37.21842005278499 40.0 37.0 40.0 33.0 40.0 40-41 37.10223483153293 38.5 37.0 40.0 33.0 40.0 42-43 36.8861267469772 37.0 37.0 40.0 33.0 40.0 44-45 36.654199405742304 37.0 37.0 40.0 33.0 40.0 46-47 36.776624135944324 37.0 37.0 40.0 33.0 40.0 48-49 36.34974388385727 37.0 37.0 40.0 33.0 40.0 50-51 36.28774333658373 37.0 37.0 40.0 33.0 40.0 52-53 36.26815648873496 37.0 37.0 40.0 33.0 40.0 54-55 35.81752479464119 37.0 37.0 40.0 30.0 40.0 56-57 35.69262142057512 37.0 37.0 40.0 27.0 40.0 58-59 35.54342490868931 37.0 35.0 40.0 27.0 40.0 60-61 35.13950012402242 37.0 33.0 40.0 27.0 40.0 62-63 34.896993801503754 37.0 33.0 38.5 27.0 40.0 64-65 34.62037753999887 37.0 33.0 37.0 27.0 40.0 66-67 33.9027273777198 37.0 33.0 37.0 27.0 40.0 68-69 33.89645998013016 37.0 33.0 37.0 27.0 40.0 70-71 33.516873119157324 37.0 33.0 37.0 27.0 38.5 72-73 32.64358170846462 37.0 33.0 37.0 22.0 37.0 74-75 31.75599835424216 37.0 33.0 37.0 18.5 37.0 76-77 29.191189092851452 33.0 27.0 33.0 15.0 37.0 78-79 31.262002450263072 33.0 33.0 37.0 15.0 37.0 80-81 31.570784812568654 37.0 33.0 37.0 15.0 37.0 82-83 31.50004199701034 37.0 33.0 37.0 15.0 37.0 84-85 31.31203860706416 35.0 33.0 37.0 15.0 37.0 86-87 31.151986196107664 33.0 33.0 37.0 15.0 37.0 88-89 31.017382496958497 33.0 33.0 37.0 15.0 37.0 90-91 30.864845910344258 33.0 33.0 37.0 15.0 37.0 92-93 30.7359944892048 33.0 33.0 37.0 15.0 37.0 94-95 30.57040634732315 33.0 33.0 37.0 15.0 37.0 96-97 30.41326009667187 33.0 27.0 37.0 15.0 37.0 98-99 30.286697348544998 33.0 27.0 37.0 15.0 37.0 100-101 28.800259758070972 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1685100784403062 2306 2 0.14290041499693018 2306 3 0.11934752632375023 2306 4 0.1728701967699351 2306 5 0.1506710869519594 2306 6 0.19652042855194196 2306 7 0.14496511457958405 2306 8 0.21263969732959964 2306 9 0.17369014784758008 2306 10-11 0.2181161819816353 2306 12-13 0.1924366221609617 2306 14-15 0.35740791339315336 2306 16-17 0.30812710721906456 2306 18-19 0.3903455219818497 2306 20-21 0.39306958488471366 2306 22-23 0.41322242682843324 2306 24-25 0.465167849797119 2306 26-27 0.4814271459144237 2306 28-29 0.4632091527164519 2306 30-31 0.45489897911836863 2306 32-33 0.5378057972351655 2306 34-35 0.46965904107248235 2306 36-37 0.47138403207429747 2306 38-39 0.4913630252218155 2306 40-41 0.5366597470058281 2306 42-43 0.48631754838921637 2306 44-45 0.4339607589059611 2306 46-47 0.13491430831187046 2306 48-49 -0.15895778412124884 2306 50-51 0.00207137727365847 2306 52-53 0.22615842733829794 2306 54-55 -0.34157033312632024 2306 56-57 -0.1939112611377567 2306 58-59 0.0789504993556136 2306 60-61 -0.23789229583181282 2306 62-63 -0.26841566538399775 2306 64-65 -0.19092949106560297 2306 66-67 -0.3595790718529983 2306 68-69 0.23511213772056294 2306 70-71 0.43595522472929815 2306 72-73 7.561151622965667E-4 2306 74-75 -0.2502591451868845 2306 76-77 -0.6382829212616699 2306 78-79 -0.3686396617595662 2306 80-81 -0.1363673471716993 2306 82-83 -0.2317756280746437 2306 84-85 -0.17009934595185072 2306 86-87 -0.11826529040249412 2306 88-89 -0.20313345566273 2306 90-91 -0.17806499658267327 2306 92-93 -0.19185857751121205 2306 94-95 -0.06658261059321191 2306 96-97 -0.002297424964840644 2306 98-99 0.024184385443955847 2306 100-101 -0.28988640066211957 2307 1 0.09056649141934514 2307 2 0.1267372384763874 2307 3 0.10027851744889205 2307 4 0.1708770805794657 2307 5 0.13667515986400502 2307 6 0.07816451465166807 2307 7 0.22356524841710268 2307 8 0.18186712875850475 2307 9 0.15973807653912075 2307 10-11 0.1621525956933283 2307 12-13 0.19280230196939385 2307 14-15 0.2929275347268785 2307 16-17 0.33872025405698736 2307 18-19 0.37161833021188073 2307 20-21 0.39665066725567044 2307 22-23 0.38047080171381964 2307 24-25 0.3413026180915608 2307 26-27 0.31825268917144456 2307 28-29 0.4043767535112224 2307 30-31 0.5246503065296224 2307 32-33 0.49060291885137275 2307 34-35 0.539116276785947 2307 36-37 0.4561878754916222 2307 38-39 0.3173163337792033 2307 40-41 0.47189946527629445 2307 42-43 0.27453334565374377 2307 44-45 0.4352105196339906 2307 46-47 0.23871268317146388 2307 48-49 0.2248005375129125 2307 50-51 0.21425066898734713 2307 52-53 0.1803167549574809 2307 54-55 -0.05098877453319517 2307 56-57 0.11101345912686611 2307 58-59 0.1573240020032145 2307 60-61 0.17821856332354713 2307 62-63 -0.01040605219713342 2307 64-65 -0.010784008546259827 2307 66-67 0.17444140415246778 2307 68-69 0.33744869625952134 2307 70-71 0.42654338748687337 2307 72-73 0.2611151082542378 2307 74-75 0.2864484002257761 2307 76-77 -0.02727710701720909 2307 78-79 0.3249905700414466 2307 80-81 0.5785738267494871 2307 82-83 0.4768581818420259 2307 84-85 0.632600983379259 2307 86-87 0.5086369540331113 2307 88-89 0.6332037405908508 2307 90-91 0.6500952895840264 2307 92-93 0.5702411030639283 2307 94-95 0.48196367350659486 2307 96-97 0.5968247629122807 2307 98-99 0.5738838185693105 2307 100-101 0.48493519660664575 2201 1 0.12174292484591831 2201 2 0.1748202293812895 2201 3 0.1213921023291391 2201 4 0.15973817716962202 2201 5 0.12039410266439887 2201 6 0.13427556906741955 2201 7 0.12789078069979354 2201 8 0.19655631898903891 2201 9 0.13083663136272605 2201 10-11 0.19043687133832066 2201 12-13 0.10384102814258966 2201 14-15 0.22982663618635257 2201 16-17 0.21515027890343674 2201 18-19 0.19739012188488658 2201 20-21 0.10602213319645415 2201 22-23 0.20624189915788094 2201 24-25 0.11058951306235798 2201 26-27 0.23781272852033908 2201 28-29 0.2537228245572223 2201 30-31 0.21240695429243317 2201 32-33 0.18642615983142719 2201 34-35 0.2533261713534003 2201 36-37 0.30683562580801294 2201 38-39 0.2654799940874284 2201 40-41 0.11775904914158275 2201 42-43 0.27857827059718687 2201 44-45 0.3050429397091463 2201 46-47 0.09464837535590931 2201 48-49 0.02804112880158982 2201 50-51 0.17772554866011347 2201 52-53 0.08688429961549815 2201 54-55 -0.1812563234260054 2201 56-57 -0.11996970096164006 2201 58-59 -0.010647467964098212 2201 60-61 -0.18666504572281184 2201 62-63 -0.23971924456033378 2201 64-65 -0.2307553091217045 2201 66-67 -0.2980522037345281 2201 68-69 -0.17351907971558234 2201 70-71 -0.0034720868763002954 2201 72-73 -0.22174646180005908 2201 74-75 3.6949555243026566E-4 2201 76-77 -0.1968949409257128 2201 78-79 0.12171790537554372 2201 80-81 0.20215456245167118 2201 82-83 0.3208201603112073 2201 84-85 0.25563050129973774 2201 86-87 0.2644853122163653 2201 88-89 0.37317091744636954 2201 90-91 0.13608158411620863 2201 92-93 0.1534166728696924 2201 94-95 0.13387346262066657 2201 96-97 0.25278294811451474 2201 98-99 0.19228942460688003 2201 100-101 0.16470369902290827 1101 1 0.09336603737678928 1101 2 0.11490511927584635 1101 3 0.051067775606568944 1101 4 0.13480598556100176 1101 5 0.1318651824224446 1101 6 0.10692150130339684 1101 7 0.0466058967209122 1101 8 0.033463605618138104 1101 9 0.10500525759577073 1101 10-11 0.0821031682754807 1101 12-13 0.08779950012448268 1101 14-15 0.09060099766349339 1101 16-17 0.19015005706573618 1101 18-19 0.087622755689992 1101 20-21 0.13626157002447314 1101 22-23 0.12447873309017865 1101 24-25 0.20197333665701223 1101 26-27 0.1817924969217799 1101 28-29 0.12244075052259262 1101 30-31 0.1673567450255362 1101 32-33 0.3469766131198 1101 34-35 0.32627261973176047 1101 36-37 0.27818336380289566 1101 38-39 0.23757783930386722 1101 40-41 0.17251184799394537 1101 42-43 0.23554057297676678 1101 44-45 0.23715054578453731 1101 46-47 0.23315525971930384 1101 48-49 0.16812424399451942 1101 50-51 0.22506837664715817 1101 52-53 0.28542904428029914 1101 54-55 0.2297187272269383 1101 56-57 0.07535100241177162 1101 58-59 0.15213555997267036 1101 60-61 0.1494483577112362 1101 62-63 0.17715461852142766 1101 64-65 -0.012400457531121845 1101 66-67 0.13656275995803213 1101 68-69 0.2882103670211862 1101 70-71 0.06818799860516833 1101 72-73 -0.009515809745103354 1101 74-75 0.2584167321941031 1101 76-77 0.12350525429146941 1101 78-79 0.22205509868492257 1101 80-81 0.40117049039877983 1101 82-83 0.5361121726749616 1101 84-85 0.5166479968999553 1101 86-87 0.47181956247887413 1101 88-89 0.4152943479446165 1101 90-91 0.412703735350366 1101 92-93 0.3291893335911098 1101 94-95 0.5241433651599685 1101 96-97 0.4131254655287755 1101 98-99 0.3716455036994155 1101 100-101 0.2469519502023445 2304 1 0.061729735709810996 2304 2 0.10124195387955126 2304 3 0.07204100483133402 2304 4 0.09689946638147973 2304 5 0.1212775577090568 2304 6 0.08988611678334735 2304 7 -0.020680308893517463 2304 8 0.0865707506884803 2304 9 0.048844106204562365 2304 10-11 0.014956238858609083 2304 12-13 0.08330935325704303 2304 14-15 0.13004459002886648 2304 16-17 0.06984400575508687 2304 18-19 0.0663097018385983 2304 20-21 0.040237293517066064 2304 22-23 0.1095289282937486 2304 24-25 0.0833325577018158 2304 26-27 -0.015256908172105454 2304 28-29 0.07845178107349682 2304 30-31 0.0031365523007451657 2304 32-33 0.06779701034357544 2304 34-35 0.1649143282277663 2304 36-37 0.05398540259572826 2304 38-39 0.08292610725950311 2304 40-41 0.15137749836266323 2304 42-43 0.08689762509301602 2304 44-45 0.052966605151219426 2304 46-47 -0.09840582660405062 2304 48-49 0.13352596054654242 2304 50-51 0.08690305781465923 2304 52-53 0.0388392971153948 2304 54-55 -0.0633437998846702 2304 56-57 0.12713029843953905 2304 58-59 0.06242751324774787 2304 60-61 0.11382765683746499 2304 62-63 0.043505008928569566 2304 64-65 -0.0968085724223684 2304 66-67 0.02595956543613198 2304 68-69 0.03583515787450864 2304 70-71 -0.04117611802122667 2304 72-73 -0.06795061794985457 2304 74-75 -1.558804352548293E-4 2304 76-77 -0.29916856983941287 2304 78-79 0.07154501316716733 2304 80-81 0.165704790454555 2304 82-83 0.24745334513576722 2304 84-85 0.34154683187547974 2304 86-87 0.144089346205714 2304 88-89 0.2845669822632644 2304 90-91 0.24927938447969922 2304 92-93 0.2817509156695621 2304 94-95 0.18239621575177623 2304 96-97 0.05122083641555619 2304 98-99 0.11879096134333977 2304 100-101 0.2880826591321366 2202 1 0.06967972778642206 2202 2 0.04663421838501591 2202 3 0.07648206611310826 2202 4 -0.014596526645938468 2202 5 0.052820864706539794 2202 6 0.05145540118784453 2202 7 0.03525176499777416 2202 8 0.06836166368962893 2202 9 0.05662402832893321 2202 10-11 0.11432576322869892 2202 12-13 0.11576215247828259 2202 14-15 0.03290932074673236 2202 16-17 -0.02795796037807463 2202 18-19 -0.022553546073027064 2202 20-21 -0.007290183674882655 2202 22-23 0.011291883039852735 2202 24-25 -0.013362680339518818 2202 26-27 0.12428718550502538 2202 28-29 0.09908542606025605 2202 30-31 0.09901539797570535 2202 32-33 0.03189852597528642 2202 34-35 -0.004380404980302899 2202 36-37 0.0189903264179776 2202 38-39 0.09130962207623838 2202 40-41 0.16127438107382375 2202 42-43 0.0577091219349839 2202 44-45 0.04257974553108568 2202 46-47 -0.03404963051141152 2202 48-49 -0.1694880412192461 2202 50-51 0.04131907551778369 2202 52-53 0.1120252647879525 2202 54-55 0.056165855334583625 2202 56-57 0.0043774290396783044 2202 58-59 -0.10651179922819409 2202 60-61 -0.07893361701760426 2202 62-63 -0.26992018859027667 2202 64-65 -0.09950210127483672 2202 66-67 0.09503046281688654 2202 68-69 0.1298641744683522 2202 70-71 0.07082767954743474 2202 72-73 -0.13966042937311585 2202 74-75 -0.1310111257208355 2202 76-77 -0.5668391491525675 2202 78-79 -0.12337708290962368 2202 80-81 0.029909168694658206 2202 82-83 0.06401295168274856 2202 84-85 0.07139509891642959 2202 86-87 -0.1356570486508737 2202 88-89 0.025040518133593537 2202 90-91 -0.016293618259531684 2202 92-93 -0.14769538071058008 2202 94-95 -0.22549732215599505 2202 96-97 -0.09824320270336173 2202 98-99 -0.0853502844402847 2202 100-101 -0.1301124204413604 1102 1 0.11473294771288067 1102 2 0.1252804990029759 1102 3 0.115895006469934 1102 4 0.04905667524208468 1102 5 0.1038670287282244 1102 6 -0.0056688012975385504 1102 7 0.032506131802868765 1102 8 0.010252536370941812 1102 9 0.0397971724939552 1102 10-11 0.03159704433684141 1102 12-13 0.02151347300955564 1102 14-15 -0.004257899490831107 1102 16-17 -0.0030370746123651315 1102 18-19 0.011637397324186338 1102 20-21 -0.023673594586568925 1102 22-23 0.03455891857949922 1102 24-25 5.306419093287218E-4 1102 26-27 0.0336354750559309 1102 28-29 0.0642101984556902 1102 30-31 -0.016985937914384408 1102 32-33 0.0505116446250824 1102 34-35 -0.02967624189191298 1102 36-37 -0.1341518909765682 1102 38-39 -0.009045460645680237 1102 40-41 0.07582832841988107 1102 42-43 -0.03867168778172214 1102 44-45 0.06788466701813434 1102 46-47 0.010102843957007224 1102 48-49 0.1311605582529367 1102 50-51 0.23562709883180588 1102 52-53 0.12889022355444268 1102 54-55 0.0661832663482258 1102 56-57 0.04986768893696336 1102 58-59 0.04271725363619083 1102 60-61 0.1073165185974645 1102 62-63 0.037173296265933686 1102 64-65 0.013120426119407114 1102 66-67 0.06755471994042495 1102 68-69 0.02922282958518707 1102 70-71 -0.04839096853703495 1102 72-73 -0.19311374445887708 1102 74-75 -0.33273667220053227 1102 76-77 -0.312555673799217 1102 78-79 -0.0645355688834286 1102 80-81 0.12539230471623952 1102 82-83 0.1289448565809721 1102 84-85 0.1182222946060314 1102 86-87 0.07449734991755363 1102 88-89 -0.04077559698824018 1102 90-91 -0.010231461463678926 1102 92-93 0.1469048522205476 1102 94-95 0.06037969229958762 1102 96-97 0.12130438543989541 1102 98-99 -0.0972092500012458 1102 100-101 -0.09324878187936392 2305 1 -0.02275735874660967 2305 2 -0.017034352663628738 2305 3 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76-77 0.30734749347721646 1213 78-79 -0.20216351311788472 1213 80-81 -0.3610354940074707 1213 82-83 -0.2543932406863121 1213 84-85 -0.24938766279533553 1213 86-87 -0.18222914189762562 1213 88-89 -0.39777772890833063 1213 90-91 -0.3395599885523346 1213 92-93 -0.2856962986631828 1213 94-95 -0.2909823871997297 1213 96-97 -0.3396434449500596 1213 98-99 -0.21773677893836663 1213 100-101 0.045309481510031446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 15.0 10 25.0 11 22.0 12 38.0 13 107.0 14 401.0 15 1187.0 16 2512.0 17 4310.0 18 5433.0 19 6515.0 20 8422.0 21 11375.0 22 15215.0 23 19977.0 24 25825.0 25 32790.0 26 43479.0 27 62237.0 28 95937.0 29 115173.0 30 78256.0 31 65275.0 32 84946.0 33 119546.0 34 183101.0 35 316632.0 36 620082.0 37 921037.0 38 207747.0 39 214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.911173109089056 23.64758421483285 21.871079257733044 13.57016341834505 2 27.702146703431552 29.07118362011604 30.58848966939166 12.638180007060749 3 34.3663859193444 17.67326760287023 32.63888592672702 15.321460551058355 4 27.25356179576194 17.393736751957263 30.91491065159042 24.437790800690376 5 36.65249705036623 16.205137021808262 31.548941609719154 15.593424318106356 6 32.15583121926508 17.021191428935154 35.11438935690766 15.708587994892115 7 30.179084438926502 26.379995511569522 26.418547454653073 17.022372594850903 8 26.91188108546523 33.89985550403631 22.8312809481875 16.356982462310967 9 35.093095560259805 23.38288543084339 23.848461662635394 17.675557346261414 10-11 26.46794315704651 29.987358243685026 27.060658775603414 16.484039823665054 12-13 21.792977638225167 28.679144376253962 27.00129764242487 22.526580343096 14-15 20.96690898066694 29.24233784232485 27.86568896751663 21.925064209491588 16-17 24.7128946570616 25.536725073133855 28.479681977639217 21.270698292165328 18-19 19.479148484367272 25.617552912952007 33.132770267166606 21.770528335514115 20-21 24.222612371531802 25.904330154247145 32.54635420016038 17.32670327406068 22-23 19.544017011430746 34.72189913292493 28.222855386105472 17.51122846953885 24-25 19.93531146940181 25.249755113095834 32.703684303171876 22.11124911433048 26-27 19.768895045533945 25.425941553285675 28.04361848865884 26.76154491252154 28-29 23.794866259208174 30.23505857926739 27.80690955812583 18.16316560339861 30-31 18.781063023076044 26.443515989705485 32.05874594302318 22.71667504419529 32-33 18.483901364771597 30.992891349797034 28.293057762950504 22.23014952248087 34-35 18.911762312670366 26.408245063054576 32.829719184365565 21.850273439909497 36-37 23.50446349475497 27.010902161402385 28.212984556911856 21.271649786930794 38-39 24.0436424269985 31.3010242751034 27.298007686820785 17.357325611077318 40-41 23.78576143860759 30.940231036053124 27.20436729535316 18.069640229986128 42-43 19.006747738395376 31.023043890813025 32.17161290830609 17.798595462485515 44-45 18.655700403483458 26.591782848959056 32.58850346926874 22.164013278288753 46-47 23.59538193376397 26.20125089715985 27.92679995898698 22.276567210089205 48-49 19.36102779426069 31.323989560234132 31.46571615734064 17.84926648816454 50-51 23.846653319448027 26.65173471188972 31.086157805596514 18.41545416306574 52-53 19.004812165116665 26.573411315355212 26.82147577766429 27.600300741863837 54-55 23.875074501229342 26.543928779367594 31.134795835051122 18.446200884351946 56-57 19.321036212987853 26.63742634893318 30.960181052635033 23.081356385443932 58-59 19.203777156432768 26.579495713063945 31.624809548348022 22.59191758215527 60-61 23.226945089767696 26.768043071300834 31.679448359767026 18.325563479164448 62-63 18.901320569647716 26.841422547672288 31.308231294336085 22.949025588343908 64-65 23.43311877462017 26.671694327001127 31.146601850642725 18.74858504773598 66-67 19.44222230388908 35.193899354642255 26.478387012519967 18.885491328948696 68-69 19.506763867959585 35.65860657183333 26.372186233389744 18.462443326817336 70-71 18.91080923534437 36.51769628601368 26.256864270258202 18.31463020838375 72-73 18.813369262508562 36.103587585567624 26.241586789875846 18.84145636204796 74-75 19.519973382167695 34.874429916710994 26.20179742556473 19.40379927555658 76-77 19.84028666764773 33.69535918847935 26.622623769547666 19.84173037432525 78-79 19.693744229281357 33.23456531682435 27.174908800497683 19.896781653396612 80-81 19.055339698155453 33.3708249310813 27.17086522251832 20.40297014824493 82-83 18.536496058186856 32.823928190361954 27.32213938020287 21.31743637124832 84-85 18.71382972143892 32.13860645145626 27.810370252739375 21.337193574365447 86-87 18.81505114847001 32.01195684485237 28.39437311051862 20.778618896158992 88-89 19.15519410336824 32.39900764706307 27.89968914324413 20.54610910632456 90-91 19.644768745477045 31.82986817541459 27.492064783717673 21.033298295390694 92-93 19.058426995049516 32.334661490539546 27.501992563938416 21.104918950472523 94-95 18.827568069423684 32.645202930628656 27.481066693179283 21.04616230676838 96-97 19.285610454862105 33.08369474516979 26.828237472924876 20.802457327043232 98-99 19.805537417599904 32.20750222303889 26.4931110403822 21.49384931897901 100-101 19.63765930826882 32.22675725949583 26.22999715516554 21.905586277069812 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 935.0 1 592.5 2 280.5 3 439.5 4 486.0 5 378.5 6 362.5 7 392.0 8 423.0 9 441.0 10 463.0 11 477.0 12 459.0 13 464.5 14 494.0 15 519.5 16 514.5 17 508.5 18 532.5 19 577.5 20 653.0 21 781.0 22 947.5 23 1159.5 24 1504.5 25 2153.5 26 3476.0 27 5684.5 28 8019.0 29 10124.0 30 12457.0 31 14568.5 32 17728.0 33 26998.0 34 44101.5 35 74535.5 36 114609.5 37 158145.5 38 206483.0 39 238552.5 40 249478.0 41 235861.0 42 212714.5 43 206488.5 44 195495.5 45 177089.0 46 167000.0 47 147432.0 48 113837.0 49 87848.5 50 69828.5 51 57180.5 52 47534.5 53 36712.0 54 27529.0 55 19977.0 56 13591.5 57 8950.5 58 6111.5 59 4341.5 60 3091.5 61 2252.0 62 1577.0 63 1080.0 64 799.5 65 573.5 66 403.5 67 311.0 68 225.5 69 153.5 70 112.0 71 81.5 72 55.0 73 39.5 74 35.5 75 26.0 76 15.0 77 12.0 78 11.0 79 9.0 80 6.5 81 2.0 82 1.5 83 3.0 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15506083660669406 2 0.0 3 0.0044949925127205 4 0.007086995494508235 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.281016432642701E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.843049297928103E-5 24-25 1.6405082163213505E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.218236151813943E-4 40-41 0.0 42-43 0.0 44-45 1.1483557514249454E-4 46-47 7.710388616710347E-4 48-49 0.0021818759277073964 50-51 3.2810164326427016E-4 52-53 0.001296001490893867 54-55 0.0022146860920338237 56-57 0.0033958520077851957 58-59 0.004806689073821557 60-61 0.0017225336271374182 62-63 7.382286973446078E-4 64-65 9.843049297928103E-4 66-67 0.00734947680911965 68-69 0.0068409192620600325 70-71 0.008579857971360664 72-73 0.005692563510635087 74-75 0.008366591903238888 76-77 0.00411767562296659 78-79 0.004002840047824095 80-81 0.0013780269017099344 82-83 0.0 84-85 4.1012705408033763E-4 86-87 0.0013780269017099344 88-89 0.004265321362435512 90-91 0.006151905811205064 92-93 0.008366591903238888 94-95 0.007562742877241426 96-97 0.009744618804948823 98-99 0.006758893851243964 100-101 0.006791704015570391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3047836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.87104657377582 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3616970819853 31.47375108726424 2 6.550136135179756 4.830207490095695 3 2.2267143853599665 2.4630386942731173 4 1.256318486329437 1.8528710968379287 5 0.8743001443233879 1.6118180670403277 6 0.5175130052753303 1.1448747672024837 7 0.37597886741563347 0.9703914031866114 8 0.2995835785631427 0.8836768062352053 9 0.20450825413747836 0.6786390026722093 >10 1.8777124724222094 13.528680040555031 >50 0.23285159997539512 6.003286204590965 >100 0.18815395671062368 15.062732114995846 >500 0.027351737446138176 6.858447494322478 >1k 0.006731219535651812 3.891714757756871 >5k 3.592602734554661E-4 0.7502374442350703 >10k+ 8.981506720639966E-5 7.995633528736135 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 241445 7.921850125794171 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6767 0.22202638199693162 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5568 0.1826869949695456 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5273 0.17300799649324963 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5047 0.16559289935547714 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4721 0.1548967857850619 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4297 0.14098527611065687 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3441 0.11289977544723535 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3218 0.10558310880244212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004363751855414793 0.0 0.0 2 0.004823094155984771 0.0 3.281016432642701E-5 3 0.0055777279354925925 0.0 3.281016432642701E-5 4 0.0058730194144304354 0.0 3.281016432642701E-5 5 0.006824514179896818 0.0 3.281016432642701E-5 6 0.007776008945363202 0.0 3.281016432642701E-5 7 0.009186846011399564 0.0 3.281016432642701E-5 8 0.010860164392047341 0.0 3.281016432642701E-5 9 0.012861584415959389 0.0 3.281016432642701E-5 10-11 0.021474252551646478 0.0 3.281016432642701E-5 12-13 0.030595478234393188 0.0 3.281016432642701E-5 14-15 0.042866479692476894 0.0 3.281016432642701E-5 16-17 0.05843490266536651 0.0 3.281016432642701E-5 18-19 0.07577507451188319 0.0 3.281016432642701E-5 20-21 0.08712739136882694 0.0 3.281016432642701E-5 22-23 0.09803677100736391 0.0 3.281016432642701E-5 24-25 0.11096397575197617 0.0 3.281016432642701E-5 26-27 0.12408804148254696 0.0 3.281016432642701E-5 28-29 0.13813079181425772 0.0 3.281016432642701E-5 30-31 0.23474032067342204 0.0 3.281016432642701E-5 32-33 0.25199846710912266 0.0 3.281016432642701E-5 34-35 0.26512253283969345 0.0 3.281016432642701E-5 36-37 0.27868953578867106 0.0 3.281016432642701E-5 38-39 0.2940939079399285 0.0 6.562032865285402E-5 40-41 0.30930141910522746 0.0 6.562032865285402E-5 42-43 0.32608381815819487 0.0 6.562032865285402E-5 44-45 0.34560586593241893 0.0 6.562032865285402E-5 46-47 0.3648654323920316 0.0 6.562032865285402E-5 48-49 0.3850108732884578 0.0 6.562032865285402E-5 50-51 0.4061898343611664 0.0 6.562032865285402E-5 52-53 0.4290257087323596 0.0 6.562032865285402E-5 54-55 0.4553558656043173 0.0 6.562032865285402E-5 56-57 0.48383508823965593 0.0 6.562032865285402E-5 58-59 0.5149227189389456 0.0 6.562032865285402E-5 60-61 0.5482906560589218 0.0 6.562032865285402E-5 62-63 0.5847755587899086 0.0 6.562032865285402E-5 64-65 0.6221463359577091 0.0 6.562032865285402E-5 66-67 0.6620763059429706 0.0 6.562032865285402E-5 68-69 0.7085683087935177 0.0 6.562032865285402E-5 70-71 0.7588170754594408 0.0 6.562032865285402E-5 72-73 0.8110672621492758 0.0 6.562032865285402E-5 74-75 0.8675007447907302 0.0 6.562032865285402E-5 76-77 0.9291674486422498 0.0 6.562032865285402E-5 78-79 0.9974454006055444 0.0 6.562032865285402E-5 80-81 1.074171969882894 0.0 6.562032865285402E-5 82-83 1.1571816856287542 0.0 6.562032865285402E-5 84-85 1.2432099364926459 0.0 9.843049297928103E-5 86-87 1.3375555640132868 0.0 9.843049297928103E-5 88-89 1.4408747714772054 0.0 9.843049297928103E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 33515 0.0 79.29588 3 GATCGGA 33720 0.0 78.99716 1 CGGAAGA 33590 0.0 78.90413 4 GAAGAGC 33675 0.0 78.7293 6 ATCGGAA 33915 0.0 78.402664 2 GGAAGAG 34650 0.0 76.596214 5 GAGCACA 41550 0.0 63.796253 9 AGAGCAC 41630 0.0 63.71929 8 AAGAGCA 42275 0.0 62.679703 7 CGAGTCG 25 0.004990359 57.091293 1 ATCGGAC 140 0.0 54.28524 2 TCGGACG 135 0.0 52.777313 3 GCCGTCT 28640 0.0 45.118885 50-51 CGTCTTC 28685 0.0 44.950226 52-53 ATGCCGT 28725 0.0 44.88542 48-49 TCGTATG 29335 0.0 44.712337 44-45 TCTCGTA 28740 0.0 44.67084 42-43 AATCTCG 28790 0.0 44.633408 40-41 CTGCTTG 28895 0.0 44.598885 58-59 GTATGCC 29245 0.0 44.500755 46-47 >>END_MODULE