##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1809825 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65375602613512 33.0 33.0 33.0 33.0 33.0 2 32.67831585926816 33.0 33.0 33.0 33.0 33.0 3 32.713105963283745 33.0 33.0 33.0 33.0 33.0 4 36.65048167640518 37.0 37.0 37.0 37.0 37.0 5 36.65660105258796 37.0 37.0 37.0 37.0 37.0 6 36.5375420275441 37.0 37.0 37.0 37.0 37.0 7 36.44363902587267 37.0 37.0 37.0 37.0 37.0 8 36.364405398311995 37.0 37.0 37.0 37.0 37.0 9 36.40144820631829 37.0 37.0 37.0 37.0 37.0 10-11 36.32724268921028 37.0 37.0 37.0 37.0 37.0 12-13 36.324274998963986 37.0 37.0 37.0 37.0 37.0 14-15 38.14376666252262 40.0 37.0 40.0 37.0 40.0 16-17 38.0667335239595 40.0 37.0 40.0 37.0 40.0 18-19 37.976719572334346 40.0 37.0 40.0 33.0 40.0 20-21 37.91790228336994 40.0 37.0 40.0 33.0 40.0 22-23 37.724792728578734 40.0 37.0 40.0 33.0 40.0 24-25 37.68056220905336 40.0 37.0 40.0 33.0 40.0 26-27 37.54072078792149 40.0 37.0 40.0 33.0 40.0 28-29 37.334517978257566 40.0 37.0 40.0 33.0 40.0 30-31 37.231770475045934 40.0 37.0 40.0 33.0 40.0 32-33 37.047260094208006 37.0 37.0 40.0 33.0 40.0 34-35 36.876434461895485 37.0 37.0 40.0 33.0 40.0 36-37 36.75108007680301 37.0 37.0 40.0 33.0 40.0 38-39 36.5947309822773 37.0 37.0 40.0 33.0 40.0 40-41 36.484417830453225 37.0 37.0 40.0 33.0 40.0 42-43 36.1262832041772 37.0 37.0 40.0 33.0 40.0 44-45 35.90254665506333 37.0 37.0 40.0 33.0 40.0 46-47 35.89380741231887 37.0 37.0 40.0 33.0 40.0 48-49 35.60527537192823 37.0 35.0 40.0 27.0 40.0 50-51 35.41586617490641 37.0 33.0 40.0 27.0 40.0 52-53 35.19233876203501 37.0 33.0 40.0 27.0 40.0 54-55 34.866948461867864 37.0 33.0 40.0 27.0 40.0 56-57 34.631684555136545 37.0 33.0 38.5 27.0 40.0 58-59 34.390297956984796 37.0 33.0 37.0 27.0 40.0 60-61 34.09051980163828 37.0 33.0 37.0 27.0 40.0 62-63 33.75775862307129 37.0 33.0 37.0 27.0 40.0 64-65 33.45397925213764 37.0 33.0 37.0 27.0 40.0 66-67 33.00365007666487 37.0 33.0 37.0 22.0 37.0 68-69 32.72989432679955 37.0 33.0 37.0 22.0 37.0 70-71 32.31040735982761 33.0 33.0 37.0 22.0 37.0 72-73 31.710604063928834 33.0 33.0 37.0 22.0 37.0 74-75 31.22014752807592 33.0 30.0 37.0 15.0 37.0 76-77 28.399660188139737 30.0 24.5 33.0 15.0 35.0 78-79 30.9994466315804 33.0 30.0 37.0 15.0 37.0 80-81 31.511896454077053 33.0 33.0 37.0 15.0 37.0 82-83 31.44840689016894 33.0 33.0 37.0 15.0 37.0 84-85 31.299679250756288 33.0 33.0 37.0 15.0 37.0 86-87 31.143656707140195 33.0 33.0 37.0 15.0 37.0 88-89 30.981414777671873 33.0 33.0 37.0 15.0 37.0 90-91 30.821557885430913 33.0 33.0 37.0 15.0 37.0 92-93 30.692357272111945 33.0 27.0 37.0 15.0 37.0 94-95 30.48503971378448 33.0 27.0 37.0 15.0 37.0 96-97 30.29649634633183 33.0 27.0 37.0 15.0 37.0 98-99 30.142711588136976 33.0 27.0 37.0 15.0 37.0 100-101 28.374103573549927 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14425739336800802 2306 2 0.18489034994029652 2306 3 0.16374017790677442 2306 4 0.20057313634307405 2306 5 0.19744341843856006 2306 6 0.17642823582821876 2306 7 0.2350244482150785 2306 8 0.25854208983936644 2306 9 0.20474661842742137 2306 10-11 0.29812191159428636 2306 12-13 0.30174434766684755 2306 14-15 0.50719407354174 2306 16-17 0.4529815625978415 2306 18-19 0.5899393699308391 2306 20-21 0.5340739315361063 2306 22-23 0.6296898633831844 2306 24-25 0.5168241168958048 2306 26-27 0.6623414970794883 2306 28-29 0.6686481458517264 2306 30-31 0.8257303912905414 2306 32-33 0.7869244850723689 2306 34-35 0.8861112678295981 2306 36-37 0.791520742530011 2306 38-39 0.7664430303430478 2306 40-41 0.9521728302005883 2306 42-43 1.0505776490279786 2306 44-45 0.967573547092222 2306 46-47 0.9567733680371404 2306 48-49 0.819344792396528 2306 50-51 1.024791737207579 2306 52-53 1.0101117641926933 2306 54-55 1.013912773517255 2306 56-57 1.0244964658499782 2306 58-59 1.0436288417442299 2306 60-61 1.0229584066405621 2306 62-63 0.851324175321146 2306 64-65 0.8895705974525683 2306 66-67 0.9250834682877738 2306 68-69 0.8212011669681658 2306 70-71 0.810533469747682 2306 72-73 0.9410334626301129 2306 74-75 0.7688432309432898 2306 76-77 0.32923064805997626 2306 78-79 0.8119420395986197 2306 80-81 0.8997412806486942 2306 82-83 0.9804403029048636 2306 84-85 1.0374260417956656 2306 86-87 1.1196145393270776 2306 88-89 1.0492206575695882 2306 90-91 1.1470037841724228 2306 92-93 1.260951733705852 2306 94-95 0.9509638089997523 2306 96-97 1.092280856859709 2306 98-99 1.092620900305942 2306 100-101 0.7327585718360083 2307 1 0.13601894704668638 2307 2 0.1078431693634272 2307 3 0.10334057672096009 2307 4 0.13357603239637683 2307 5 0.12847524027429813 2307 6 0.13248559679644956 2307 7 0.16099392594939843 2307 8 0.08694467139977746 2307 9 0.20951358246719565 2307 10-11 0.2519800198025308 2307 12-13 0.18971098300687572 2307 14-15 0.3356365160793331 2307 16-17 0.3838769372424764 2307 18-19 0.3663463729934904 2307 20-21 0.32835653870975534 2307 22-23 0.3995900729599384 2307 24-25 0.46806570967913075 2307 26-27 0.4924473688569506 2307 28-29 0.5027513581865506 2307 30-31 0.6112116435997876 2307 32-33 0.6610777587062202 2307 34-35 0.5387075205911174 2307 36-37 0.6115440867535682 2307 38-39 0.6073205646452067 2307 40-41 0.8017454234279953 2307 42-43 0.6906690533148705 2307 44-45 0.6671027787310067 2307 46-47 0.7385102436826472 2307 48-49 0.7236661822568138 2307 50-51 0.7546940113785325 2307 52-53 0.692434851721778 2307 54-55 0.5268844553591947 2307 56-57 0.5948360149405616 2307 58-59 0.766989571833804 2307 60-61 0.6983891223852936 2307 62-63 0.6465833764356219 2307 64-65 0.47425115515873983 2307 66-67 0.704975240624357 2307 68-69 0.47285148807899446 2307 70-71 0.5630645140388495 2307 72-73 0.603017997593053 2307 74-75 0.6225307035069285 2307 76-77 0.1966857444136707 2307 78-79 0.6014367301675634 2307 80-81 0.8513145372036526 2307 82-83 0.7776434932139402 2307 84-85 0.9980906673758874 2307 86-87 0.5593128019506146 2307 88-89 0.6785705505837463 2307 90-91 0.6505468117976214 2307 92-93 0.9167861798247969 2307 94-95 0.8292562362828555 2307 96-97 0.9383712360040377 2307 98-99 0.8287573290996733 2307 100-101 0.604056675220054 2201 1 0.07397837589108747 2201 2 0.08150739078791247 2201 3 0.07240660771925178 2201 4 0.04599852484072642 2201 5 0.07210602289369206 2201 6 0.09088526382730322 2201 7 0.0774158319139886 2201 8 0.16273440895008662 2201 9 0.08660634559086589 2201 10-11 0.178604072093826 2201 12-13 0.08189093769659195 2201 14-15 0.2718608635023685 2201 16-17 0.241092293552569 2201 18-19 0.3490851861287467 2201 20-21 0.324168884108488 2201 22-23 0.38946691221246255 2201 24-25 0.34777398330030707 2201 26-27 0.39121978160626014 2201 28-29 0.30577503967128905 2201 30-31 0.3548556693103109 2201 32-33 0.37804877998502917 2201 34-35 0.41263447548420373 2201 36-37 0.34434887886068566 2201 38-39 0.3982514120611427 2201 40-41 0.4082941319901252 2201 42-43 0.2228369946290627 2201 44-45 0.28698042202834273 2201 46-47 0.3669930584070684 2201 48-49 0.4187702587640416 2201 50-51 0.5317494100665172 2201 52-53 0.41627832784400454 2201 54-55 0.23331137477971708 2201 56-57 0.2668557282276325 2201 58-59 0.4952869832824476 2201 60-61 0.2955241215103044 2201 62-63 0.1946879590181183 2201 64-65 0.2001570062401825 2201 66-67 0.38721389058580513 2201 68-69 0.43748959696558387 2201 70-71 0.2863541451714653 2201 72-73 0.1303039679569018 2201 74-75 0.39040296443366174 2201 76-77 0.25572368114007205 2201 78-79 0.48940041571982107 2201 80-81 0.5620877918351859 2201 82-83 0.5175993180456793 2201 84-85 0.7417519564016253 2201 86-87 0.482339936098402 2201 88-89 0.6030087833878319 2201 90-91 0.6033973871626301 2201 92-93 0.5514270319231187 2201 94-95 0.36400847223690036 2201 96-97 0.7037296744544044 2201 98-99 0.36922514129372175 2201 100-101 0.3613540913983826 1101 1 0.0936007022533829 1101 2 0.0804939722962672 1101 3 0.06474582164921117 1101 4 0.14783539994619588 1101 5 0.08014932900925231 1101 6 0.10149283893580474 1101 7 0.12437838908645915 1101 8 0.065997945356564 1101 9 0.05418993530782501 1101 10-11 0.1555081262005089 1101 12-13 0.04823436627048494 1101 14-15 0.08875903268526741 1101 16-17 0.08202366928332339 1101 18-19 0.13707193639594095 1101 20-21 0.30188449516695215 1101 22-23 0.29065060405015686 1101 24-25 0.35811414054893476 1101 26-27 0.27430603468394565 1101 28-29 0.3457258832491519 1101 30-31 0.41948987369890745 1101 32-33 0.4316833694899671 1101 34-35 0.2373340631068217 1101 36-37 0.33006776989872577 1101 38-39 0.5196876796347851 1101 40-41 0.37911486249956994 1101 42-43 0.397512350212601 1101 44-45 0.3116531813219794 1101 46-47 0.42484164278000236 1101 48-49 0.3109617005270948 1101 50-51 0.39790244404763087 1101 52-53 0.4865661698511161 1101 54-55 0.395633799368305 1101 56-57 0.27777875255920037 1101 58-59 0.37575660885418216 1101 60-61 0.48835208701426325 1101 62-63 0.3846143541994067 1101 64-65 0.3438551615762009 1101 66-67 0.3168085664606508 1101 68-69 0.34130095054132426 1101 70-71 0.31006195005162596 1101 72-73 0.47509602677152074 1101 74-75 0.3603292730719474 1101 76-77 0.16646518153600454 1101 78-79 0.29354084904060684 1101 80-81 0.6157725762782214 1101 82-83 0.5717973024592844 1101 84-85 0.5774243305789213 1101 86-87 0.5751315535437378 1101 88-89 0.450803018758819 1101 90-91 0.5499815350819226 1101 92-93 0.46401752344407043 1101 94-95 0.48973034900832957 1101 96-97 0.4415649744773411 1101 98-99 0.4687870173728079 1101 100-101 0.4216687184629748 2304 1 0.07638122616865672 2304 2 0.12756754490175126 2304 3 0.12168160979067721 2304 4 0.11520889852536698 2304 5 0.14246396472814382 2304 6 0.11972361000345444 2304 7 -0.01806018101168405 2304 8 0.21248169538641548 2304 9 0.04237318625703779 2304 10-11 0.08392348546693285 2304 12-13 0.06192863758887057 2304 14-15 0.1421773768837582 2304 16-17 0.11013695342496987 2304 18-19 0.19347163073889817 2304 20-21 0.28902719879901895 2304 22-23 0.21859732898270323 2304 24-25 0.0962245591558073 2304 26-27 0.1313349527057781 2304 28-29 0.24796577626918292 2304 30-31 0.09299263177612715 2304 32-33 0.2691168055049573 2304 34-35 0.21443247034707724 2304 36-37 0.08570690355204391 2304 38-39 0.15922788069980953 2304 40-41 0.2844334144493246 2304 42-43 0.3326658951179198 2304 44-45 0.42889773083603444 2304 46-47 0.2649692784750073 2304 48-49 0.27977572731984424 2304 50-51 0.18119219737283743 2304 52-53 0.3258628004598094 2304 54-55 0.07161405105778584 2304 56-57 0.2699462908260344 2304 58-59 0.2813939515482602 2304 60-61 0.281856874845289 2304 62-63 0.24277396448667332 2304 64-65 0.1633664684988574 2304 66-67 0.21520088205154053 2304 68-69 0.14003951825151972 2304 70-71 0.09191177214967183 2304 72-73 0.03851508735370501 2304 74-75 0.002634715200088067 2304 76-77 -0.27712079644713583 2304 78-79 0.2664884097541247 2304 80-81 0.30726551989882367 2304 82-83 0.3269642198602476 2304 84-85 0.4866023996860669 2304 86-87 0.27655238120570047 2304 88-89 0.3055007048394849 2304 90-91 0.11744577559580804 2304 92-93 0.0882527838495406 2304 94-95 0.1218135927936963 2304 96-97 0.20533088416523526 2304 98-99 0.19301750188697753 2304 100-101 0.15100940666891205 2202 1 0.020498808270737356 2202 2 0.09591711092573973 2202 3 0.08084369375895761 2202 4 0.11753006286203771 2202 5 0.11828388124573053 2202 6 0.07643312213932774 2202 7 0.11518701967737854 2202 8 0.09458819067120317 2202 9 0.07951954549641727 2202 10-11 0.055118741545904015 2202 12-13 0.1294030738617593 2202 14-15 0.09310385494202222 2202 16-17 -0.04215439576559987 2202 18-19 -0.03342052677341201 2202 20-21 0.006136123466077947 2202 22-23 -0.01150658232276669 2202 24-25 0.025066848075617543 2202 26-27 0.12024592688806734 2202 28-29 0.04853115978030331 2202 30-31 0.05201502626364629 2202 32-33 0.08057686425183874 2202 34-35 0.041092732278656285 2202 36-37 0.022315485897060228 2202 38-39 0.3319641381259899 2202 40-41 0.2248765558614565 2202 42-43 -0.004918305063178252 2202 44-45 -0.1714346528296815 2202 46-47 -0.17665211334018238 2202 48-49 -0.11939773057454062 2202 50-51 0.18058982099526588 2202 52-53 0.1636225787234551 2202 54-55 0.012410557164365343 2202 56-57 0.1931446830166763 2202 58-59 0.034024862124901745 2202 60-61 0.1251793863848576 2202 62-63 0.02569651136271034 2202 64-65 -0.011112318576827818 2202 66-67 0.0023135612184859156 2202 68-69 0.10054162739704253 2202 70-71 0.21184209304898616 2202 72-73 -0.18204190374592244 2202 74-75 -0.31390882595585623 2202 76-77 -0.5443561594189319 2202 78-79 -0.30911783154979133 2202 80-81 0.005811203328224934 2202 82-83 -0.11003472004566817 2202 84-85 -0.23240751661067094 2202 86-87 0.006729726111725398 2202 88-89 0.10189141576507765 2202 90-91 -0.1661713537577576 2202 92-93 -0.3642351244523212 2202 94-95 -0.25745162949477773 2202 96-97 -0.0012124361377736648 2202 98-99 -0.029783355022406255 2202 100-101 -0.3649626775202286 1102 1 0.07020267437606975 1102 2 0.08128303019618954 1102 3 0.020630549090491 1102 4 -0.04167833380046204 1102 5 -0.006186507606543046 1102 6 0.020932115597922518 1102 7 0.027718796346242414 1102 8 0.038706088277471906 1102 9 0.06951690024285284 1102 10-11 0.038559005891919185 1102 12-13 0.024252002982414922 1102 14-15 -0.1400595544759753 1102 16-17 -0.14882312483334914 1102 18-19 -0.1037775463348396 1102 20-21 -0.09359441934891777 1102 22-23 -0.11270546503939727 1102 24-25 -0.0391455613020355 1102 26-27 -0.03470764667016368 1102 28-29 0.0037748145343670103 1102 30-31 0.0559829066085058 1102 32-33 0.09936264803150863 1102 34-35 -0.12542822126800246 1102 36-37 0.027489422527544605 1102 38-39 0.0030223740557033807 1102 40-41 0.024399961872013876 1102 42-43 -0.001961449597146725 1102 44-45 -0.1494566308516596 1102 46-47 -0.0126508758995314 1102 48-49 -0.04587761543876212 1102 50-51 0.036228470391563405 1102 52-53 0.03773989347724438 1102 54-55 -0.1944101618962577 1102 56-57 -0.24518055763414992 1102 58-59 -0.28651710101191696 1102 60-61 -0.19859068279524905 1102 62-63 -0.22465207925515784 1102 64-65 -0.057702407394579325 1102 66-67 -0.16769778198747076 1102 68-69 -0.06656442036995713 1102 70-71 -0.17168828925740343 1102 72-73 -0.23573606759920196 1102 74-75 -0.5906292495551071 1102 76-77 -0.791770311973842 1102 78-79 -0.36818320518064596 1102 80-81 -0.4593700862104697 1102 82-83 -0.2079494532113202 1102 84-85 -0.3148138903267146 1102 86-87 -0.3549930064800364 1102 88-89 -0.4790959363361047 1102 90-91 -0.4827006017435558 1102 92-93 -0.3294881496808628 1102 94-95 -0.39910944128723 1102 96-97 -0.32608544154933483 1102 98-99 -0.47624611299067254 1102 100-101 -0.518250638029734 2305 1 0.06648966056174999 2305 2 0.05938498180768903 2305 3 0.057015653273687406 2305 4 0.03070928091365488 2305 5 -0.04920270193852616 2305 6 -0.033653697982998665 2305 7 -0.06631894862886156 2305 8 -0.07965066558813305 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31.0 13 155.0 14 708.0 15 2107.0 16 4351.0 17 6774.0 18 6940.0 19 6727.0 20 7160.0 21 8123.0 22 9432.0 23 11275.0 24 14220.0 25 17358.0 26 21695.0 27 29039.0 28 42722.0 29 61016.0 30 57475.0 31 56542.0 32 74025.0 33 105643.0 34 161024.0 35 265070.0 36 414918.0 37 376269.0 38 48973.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20197498208461 23.219901444575715 21.9167674263532 12.661356146986478 2 33.37676294669374 23.663282361554295 30.259831751688697 12.700122940063267 3 38.38746913228924 15.864829127294048 29.078115684084832 16.66958605633188 4 33.277209509479256 15.397397011309677 31.03770868917256 20.28768479003851 5 33.73243269376873 14.535272747365077 35.10350448247759 16.6287900763886 6 30.054066000856437 14.84126918348459 38.44951859986463 16.655146215794346 7 35.810534167668145 19.4254693133314 26.571298329949027 18.19269818905143 8 32.67448510215076 29.21304546019643 21.14016548561325 16.97230395203956 9 34.362935642948905 24.81123865567113 21.89548713273383 18.93033856864614 10-11 27.619023938778607 25.918168883731852 27.572610611523213 18.89019656596632 12-13 25.545957632908607 24.672392267869068 26.24395348830784 23.537696610914484 14-15 24.87580290912105 26.02583122677607 26.01892448164878 23.079441382454107 16-17 26.32160568010719 24.674816625916872 26.946113574516872 22.057464119459063 18-19 23.824375284903237 24.496401585788682 28.817758623071292 22.861464506236793 20-21 25.348859696379485 24.998107551835123 29.15096763499233 20.502065116793062 22-23 23.720834501726 29.75446487155508 25.738435675321753 20.78626495139717 24-25 23.771980037837935 24.527329729299645 28.39690489240943 23.303785340452997 26-27 22.684209799289988 24.76960479604382 26.5033912118575 26.0427941928087 28-29 24.86425483126822 27.06944041550979 25.956708521542136 22.10959623167986 30-31 22.452833837525727 25.28852237094747 28.438744077466055 23.819899714060753 32-33 22.85290014227895 27.18514220988217 25.91256060668849 24.04939704115039 34-35 23.226997085353556 26.87547138535494 28.287569240119904 21.609962289171605 36-37 22.65127843852306 27.714834307184393 28.589062478416423 21.04482477587612 38-39 22.627991920705554 26.067383260632393 27.34992014361717 23.954704675044887 40-41 25.424170845247467 25.391239484480543 24.843479341925324 24.341110328346662 42-43 23.17818021079386 27.381597668282847 27.328553865705246 22.111668255218046 44-45 22.705071906222848 25.13984106176158 27.260656777114882 24.894430254900687 46-47 25.13860601633545 25.0408883604099 25.40902174045174 24.41148388280291 48-49 22.93707831580774 27.12758418626779 27.229392056444386 22.70594544148009 50-51 25.043996209557328 24.84287068015239 26.352555371681635 23.760577738608642 52-53 22.78229148478613 24.908221700125317 24.520498442918427 27.78898837217012 54-55 25.17040707620357 24.935379028657565 27.26145772092139 22.632756174217477 56-57 22.904058480892566 24.959310756962203 26.981492230848016 25.155138531297215 58-59 22.51775822293442 25.06753788559035 27.29450607136443 25.12019782011079 60-61 24.527959412134585 25.08721948588851 27.358738690751807 23.026082411225097 62-63 22.689235124974307 24.942949875456677 27.42102494623751 24.946790053331505 64-65 25.29028703944931 25.517107243963228 26.99461571380539 22.19799000278207 66-67 23.05312372733095 29.502713461654288 24.75994387961193 22.684218931402828 68-69 22.630094551426396 28.56297414548133 25.500068243242126 23.30686305985015 70-71 22.705350048019913 29.04104956384217 25.15154730209884 23.10205308603908 72-73 22.940907335235288 29.118420172877858 25.233171816366866 22.707500675519984 74-75 23.494137185898964 28.5182893994121 25.288526600313816 22.69904681437512 76-77 23.51223350497833 27.819640689313346 25.24181610317045 23.42630970253787 78-79 23.369426776228945 27.881604445262237 25.440434491672043 23.308534286836778 80-81 23.519763288107217 28.206481949613078 25.366187883158037 22.907566879121667 82-83 23.068169574406365 27.905073694970504 25.477905322337797 23.54885140828533 84-85 22.890847959598084 28.06024372656874 25.282639385793576 23.766268928039604 86-87 22.51222648235911 28.263079752967315 25.460313039941962 23.764380724731616 88-89 22.582186993195176 28.393806862292752 25.64073944119199 23.38326670332008 90-91 23.016275825637113 27.96569171222879 25.166926054665712 23.851106407468382 92-93 22.639689026433803 27.703867801421417 24.891119741897906 24.765323430246873 94-95 22.885474543912338 28.159375802971237 24.417453866280596 24.53769578683583 96-97 22.703084142516428 28.886344496486604 24.231893123730412 24.178678237266556 98-99 22.895120085252003 27.68408020474185 24.824065511646033 24.59673419836011 100-101 23.486117319932962 27.948677709743215 24.290808260591117 24.274396709732713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2994.0 1 1765.5 2 642.5 3 1886.5 4 2297.5 5 1326.5 6 1211.5 7 1428.5 8 1509.5 9 1473.0 10 1339.5 11 1206.0 12 1150.5 13 1108.5 14 1093.5 15 1100.0 16 1102.5 17 1115.0 18 1187.5 19 1276.0 20 1315.0 21 1390.0 22 1472.5 23 1550.5 24 1716.5 25 1894.5 26 2095.0 27 2428.5 28 2804.5 29 3329.0 30 4067.0 31 5013.0 32 6290.0 33 8039.5 34 10896.0 35 16081.0 36 22858.0 37 31539.5 38 40842.5 39 46799.5 40 53859.5 41 58234.5 42 62537.0 43 73032.0 44 78270.5 45 81392.0 46 91231.0 47 96695.5 48 93201.0 49 90152.0 50 87164.0 51 84465.0 52 82239.5 53 79474.0 54 74273.0 55 65028.0 56 56045.0 57 49703.5 58 45901.5 59 39773.0 60 30827.5 61 22791.0 62 16021.5 63 11230.0 64 8375.0 65 6919.0 66 6460.0 67 5910.0 68 4962.5 69 3830.0 70 2685.0 71 1879.0 72 1571.0 73 1329.0 74 879.0 75 521.5 76 389.5 77 309.5 78 257.5 79 198.0 80 152.5 81 126.0 82 99.5 83 81.0 84 56.5 85 38.5 86 33.0 87 26.0 88 15.5 89 12.5 90 7.0 91 3.0 92 4.5 93 6.0 94 3.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15029077396985896 2 0.0 3 0.004751840647576423 4 0.007514538698492948 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.762698050916525E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3813490254582626E-4 24-25 5.52539610183305E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.459284737474618E-4 40-41 0.0 42-43 0.0 44-45 1.3813490254582626E-4 46-47 0.0010498252593482795 48-49 0.002403547304297377 50-51 5.52539610183305E-4 52-53 0.0016023648695315845 54-55 0.0022654124017515505 56-57 0.003950658212810631 58-59 0.00552539610183305 60-61 0.0012708411034216016 62-63 7.183014932382965E-4 64-65 0.0011879601618941058 66-67 0.007956570386639593 68-69 0.006740983244236321 70-71 0.00878537980191455 72-73 0.0059674277899796945 74-75 0.008647244899368724 76-77 0.004530824803503101 78-79 0.00430980895942978 80-81 0.001657618830549915 82-83 0.0 84-85 5.52539610183305E-4 86-87 0.0013260950644399321 88-89 0.00442031688146644 90-91 0.0064370864586355036 92-93 0.008481483016313731 94-95 0.00773555454256627 96-97 0.01000096694431782 98-99 0.007265895873910461 100-101 0.006851491166272982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1809825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.50853911637503 #Duplication Level Percentage of deduplicated Percentage of total 1 82.133563783896 39.02045627794506 2 8.278361885765309 7.865857589387787 3 2.9857427780913777 4.2554483269316545 4 1.5496103102967909 2.944788881674926 5 0.8552882080326998 2.0316746643547914 6 0.6829281955990366 1.946695253657535 7 0.5800342534555638 1.928960601339173 8 0.4106001971282551 1.5605612421167205 9 0.28369057585499774 1.2129952337958705 >10 1.9927221631390828 17.748061081463852 >50 0.15079787685730717 4.876227424978246 >100 0.09186745191316033 7.863977861524759 >500 0.0035120406102246886 1.1245972829965227 >1k 0.0010478757114231038 0.9619858442169458 >5k 0.0 0.0 >10k+ 2.324036487448724E-4 4.657712433616092 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 70932 3.919273962952219 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13438 0.7425027281643253 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3935 0.21742433660713054 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3649 0.20162170375588798 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2221 0.12271904742171204 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002762698050916525 0.0 0.0 2 0.0031494757780448387 0.0 0.0 3 0.0034809995441548217 0.0 0.0 4 0.003757269349246474 0.0 0.0 5 0.004862348569613084 0.0 0.0 6 0.005746411945906372 0.0 0.0 7 0.006354205517108007 0.0 0.0 8 0.007404030776456287 0.0 0.0 9 0.009116903568024533 0.0 0.0 10-11 0.017929910350448247 0.0 0.0 12-13 0.03409169394830992 0.0 0.0 14-15 0.055005318193748015 0.0 0.0 16-17 0.08735651236998052 0.0 0.0 18-19 0.12479107095989944 0.0 0.0 20-21 0.13633914881273052 0.0 0.0 22-23 0.14498639371209923 0.0 0.0 24-25 0.15379940049452295 0.0 0.0 26-27 0.16261240727694665 0.0 0.0 28-29 0.17341455665603028 0.0 0.0 30-31 0.23057477932949316 0.0 0.0 32-33 0.2432831903637092 0.0 0.0 34-35 0.25256585581478874 0.0 0.0 36-37 0.2618485212658682 0.0 0.0 38-39 0.2698050916525079 0.0 0.0 40-41 0.27897724918155076 0.0 0.0 42-43 0.2883151685936486 0.0 0.0 44-45 0.29699004047352645 0.0 0.0 46-47 0.30770930891108256 0.0 0.0 48-49 0.31862196621220285 0.0 0.0 50-51 0.32981089331841473 0.0 0.0 52-53 0.34276794717721326 0.0 0.0 54-55 0.35669194535383253 0.0 0.0 56-57 0.3718315306728551 0.0 0.0 58-59 0.3886011078419184 0.0 0.0 60-61 0.4062823753677842 0.0 0.0 62-63 0.42650532510049316 0.0 0.0 64-65 0.44813725083916955 0.0 0.0 66-67 0.47206221596010667 0.0 0.0 68-69 0.497202768223447 0.0 0.0 70-71 0.5249126296741398 0.0 0.0 72-73 0.557540093655464 0.0 0.0 74-75 0.5958863426021852 0.0 0.0 76-77 0.6385424005083364 0.0 0.0 78-79 0.6878013067561781 0.0 0.0 80-81 0.7448233945270952 0.0 0.0 82-83 0.804635807329438 0.0 0.0 84-85 0.8716588620446728 0.0 0.0 86-87 0.9479645822109872 0.0 0.0 88-89 1.0257897863053058 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10090 0.0 70.56555 4 GATCGGA 10195 0.0 70.363075 1 ATCGGAA 10185 0.0 69.95592 2 TCGGAAG 10340 0.0 68.90725 3 GAAGAGC 10565 0.0 67.34239 6 GGAAGAG 12735 0.0 55.94207 5 GAGCACA 13765 0.0 51.928593 9 AAGAGCA 13995 0.0 50.80368 7 AGAGCAC 14380 0.0 49.41047 8 GATCTCG 8120 0.0 42.583324 40-41 GTATGCC 8295 0.0 42.576584 46-47 TATGCCG 8245 0.0 42.345078 48-49 CTCGTAT 8125 0.0 42.063137 44-45 TCGTATG 8495 0.0 41.964447 44-45 TACTCGA 8285 0.0 41.85049 36-37 GCCGTCT 8510 0.0 41.80791 50-51 TCGATCT 8240 0.0 41.733192 38-39 CACATTA 8450 0.0 41.649303 30-31 ACTCGAT 8455 0.0 41.626972 36-37 TCTCGTA 8335 0.0 41.573246 42-43 >>END_MODULE