FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809825
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT709323.919273962952219TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134380.7425027281643253No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA39350.21742433660713054No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC36490.20162170375588798No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT22210.12271904742171204TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA100900.070.565554
GATCGGA101950.070.3630751
ATCGGAA101850.069.955922
TCGGAAG103400.068.907253
GAAGAGC105650.067.342396
GGAAGAG127350.055.942075
GAGCACA137650.051.9285939
AAGAGCA139950.050.803687
AGAGCAC143800.049.410478
GATCTCG81200.042.58332440-41
GTATGCC82950.042.57658446-47
TATGCCG82450.042.34507848-49
CTCGTAT81250.042.06313744-45
TCGTATG84950.041.96444744-45
TACTCGA82850.041.8504936-37
GCCGTCT85100.041.8079150-51
TCGATCT82400.041.73319238-39
CACATTA84500.041.64930330-31
ACTCGAT84550.041.62697236-37
TCTCGTA83350.041.57324642-43
Produced by FastQC (version 0.11.2)