##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7491522 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67589656681246 33.0 33.0 33.0 33.0 33.0 2 32.709815175073906 33.0 33.0 33.0 33.0 33.0 3 32.73955853029598 33.0 33.0 33.0 33.0 33.0 4 36.68023533802611 37.0 37.0 37.0 37.0 37.0 5 36.67632158058136 37.0 37.0 37.0 37.0 37.0 6 36.55929409270907 37.0 37.0 37.0 37.0 37.0 7 36.48512144795143 37.0 37.0 37.0 37.0 37.0 8 36.42417735675074 37.0 37.0 37.0 37.0 37.0 9 36.446863934992116 37.0 37.0 37.0 37.0 37.0 10-11 36.392682621768984 37.0 37.0 37.0 37.0 37.0 12-13 36.38450017232813 37.0 37.0 37.0 37.0 37.0 14-15 38.25948485501344 40.0 37.0 40.0 37.0 40.0 16-17 38.22302437341838 40.0 37.0 40.0 37.0 40.0 18-19 38.21261347160163 40.0 37.0 40.0 37.0 40.0 20-21 38.158151974992535 40.0 37.0 40.0 37.0 40.0 22-23 38.021749852700154 40.0 37.0 40.0 35.0 40.0 24-25 38.01560690871628 40.0 37.0 40.0 35.0 40.0 26-27 37.917719189772114 40.0 37.0 40.0 33.0 40.0 28-29 37.76344866904215 40.0 37.0 40.0 33.0 40.0 30-31 37.72498265372511 40.0 37.0 40.0 33.0 40.0 32-33 37.570676425965246 40.0 37.0 40.0 33.0 40.0 34-35 37.45710097360723 40.0 37.0 40.0 33.0 40.0 36-37 37.301013665847876 40.0 37.0 40.0 33.0 40.0 38-39 37.23491121029879 40.0 37.0 40.0 33.0 40.0 40-41 37.2054001176263 40.0 37.0 40.0 33.0 40.0 42-43 37.002746170938295 38.5 37.0 40.0 33.0 40.0 44-45 36.87195986342962 37.0 37.0 40.0 33.0 40.0 46-47 36.84722236683013 37.0 37.0 40.0 33.0 40.0 48-49 36.60735415313471 37.0 37.0 40.0 33.0 40.0 50-51 36.47021199430503 37.0 37.0 40.0 33.0 40.0 52-53 36.32022064408274 37.0 37.0 40.0 33.0 40.0 54-55 36.10164236319402 37.0 37.0 40.0 33.0 40.0 56-57 35.92187955932052 37.0 37.0 40.0 30.0 40.0 58-59 35.71467540240822 37.0 37.0 40.0 27.0 40.0 60-61 35.39808059029927 37.0 33.0 40.0 27.0 40.0 62-63 35.14337340262766 37.0 33.0 38.5 27.0 40.0 64-65 34.864737232300726 37.0 33.0 37.0 27.0 40.0 66-67 34.45629739857935 37.0 33.0 37.0 27.0 40.0 68-69 34.19831050352652 37.0 33.0 37.0 27.0 40.0 70-71 33.7966769636397 37.0 33.0 37.0 27.0 40.0 72-73 33.30516756141142 37.0 33.0 37.0 27.0 37.0 74-75 32.809185036098135 37.0 33.0 37.0 22.0 37.0 76-77 30.11166422524021 33.0 30.0 35.0 22.0 37.0 78-79 32.519403934207226 35.0 33.0 37.0 24.5 37.0 80-81 32.93963316132556 37.0 33.0 37.0 27.0 37.0 82-83 32.87141731680158 37.0 33.0 37.0 27.0 37.0 84-85 32.69283671595706 37.0 33.0 37.0 24.5 37.0 86-87 32.5221583144253 37.0 33.0 37.0 22.0 37.0 88-89 32.38677440979283 33.0 33.0 37.0 22.0 37.0 90-91 32.23987201799581 33.0 33.0 37.0 22.0 37.0 92-93 32.109231208291185 33.0 33.0 37.0 22.0 37.0 94-95 31.93462750292931 33.0 33.0 37.0 22.0 37.0 96-97 31.769314566519327 33.0 33.0 37.0 22.0 37.0 98-99 31.623678739780782 33.0 33.0 37.0 18.5 37.0 100-101 30.009123513219343 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16275438254322694 2306 2 0.13888321661656988 2306 3 0.10905542274119284 2306 4 0.18844190862387222 2306 5 0.16241441696611503 2306 6 0.1775505727664637 2306 7 0.18258049001844512 2306 8 0.23610884538082644 2306 9 0.23162590233262392 2306 10-11 0.2516393744343972 2306 12-13 0.30293335709712466 2306 14-15 0.4933649304369041 2306 16-17 0.3894796286014426 2306 18-19 0.5031408811704736 2306 20-21 0.4334134895468864 2306 22-23 0.4712738715573437 2306 24-25 0.5258534117929088 2306 26-27 0.5471435223112167 2306 28-29 0.5716653471609874 2306 30-31 0.551818382471275 2306 32-33 0.5918611843349666 2306 34-35 0.6159097066146373 2306 36-37 0.6221921010456981 2306 38-39 0.6057134671476163 2306 40-41 0.6463401967419102 2306 42-43 0.6543609237218178 2306 44-45 0.6346557398967647 2306 46-47 0.6230436436621218 2306 48-49 0.560602667505016 2306 50-51 0.603544837333871 2306 52-53 0.6811038783827641 2306 54-55 0.6966973362728197 2306 56-57 0.5445773869046846 2306 58-59 0.5691330276216249 2306 60-61 0.6704115424010411 2306 62-63 0.7250121431839958 2306 64-65 0.6488140551712291 2306 66-67 0.6330920441571095 2306 68-69 0.7115166310141419 2306 70-71 0.7821870979528782 2306 72-73 0.6320721235560711 2306 74-75 0.5246637870026873 2306 76-77 0.22161703392623977 2306 78-79 0.38443934650301514 2306 80-81 0.6381898206149685 2306 82-83 0.701502244461615 2306 84-85 0.783957598440459 2306 86-87 0.6781667946375762 2306 88-89 0.6647592895142793 2306 90-91 0.6021052091153791 2306 92-93 0.5334266655810751 2306 94-95 0.5071582374907635 2306 96-97 0.6644418653328898 2306 98-99 0.7231617302053905 2306 100-101 0.42589839188740086 2307 1 0.09758000905350173 2307 2 0.1413014664494412 2307 3 0.12974665831411158 2307 4 0.1672941743650611 2307 5 0.12633091018382459 2307 6 0.17093643861258556 2307 7 0.18472098446255103 2307 8 0.19706651277289922 2307 9 0.18414474465764386 2307 10-11 0.1416267470343655 2307 12-13 0.16517097110327228 2307 14-15 0.3323046357870396 2307 16-17 0.342633870949534 2307 18-19 0.2521151617326467 2307 20-21 0.31028474300994446 2307 22-23 0.3988211413364411 2307 24-25 0.3221429869970578 2307 26-27 0.36096687067415445 2307 28-29 0.43411586087017184 2307 30-31 0.3991969721534687 2307 32-33 0.4323795715046401 2307 34-35 0.3915276234571152 2307 36-37 0.41037985183550774 2307 38-39 0.4115551607996153 2307 40-41 0.3497087413984943 2307 42-43 0.26752565992926236 2307 44-45 0.38110774076282894 2307 46-47 0.33481021929771515 2307 48-49 0.3730080805347811 2307 50-51 0.29105264056136093 2307 52-53 0.2304874036557365 2307 54-55 0.1806814982891609 2307 56-57 0.2953774646603833 2307 58-59 0.2174496442686049 2307 60-61 0.3092633577280779 2307 62-63 0.21335777598425665 2307 64-65 0.19892290525601908 2307 66-67 0.16172153269208422 2307 68-69 0.16695188516624881 2307 70-71 0.30386770375199745 2307 72-73 0.18873307707490738 2307 74-75 0.24709974522321687 2307 76-77 -0.1518254616209802 2307 78-79 0.1399963705140408 2307 80-81 0.3646593560246316 2307 82-83 0.26352923059043576 2307 84-85 0.44367350564565555 2307 86-87 0.2840774727716351 2307 88-89 0.35630357391683276 2307 90-91 0.4169026175746282 2307 92-93 0.3549696184962059 2307 94-95 0.3126198448212918 2307 96-97 0.3803916623304531 2307 98-99 0.2690206660269041 2307 100-101 0.1729346865752035 2201 1 0.1209906066101496 2201 2 0.14136332305806576 2201 3 0.10433896112972718 2201 4 0.13322752118661896 2201 5 0.09123221739361753 2201 6 0.10139195697840364 2201 7 0.09470099615888472 2201 8 0.12725902805402 2201 9 0.1450160263165401 2201 10-11 0.16772973396088275 2201 12-13 0.14887950844024544 2201 14-15 0.27186576193730616 2201 16-17 0.2113705301976765 2201 18-19 0.1954954402050717 2201 20-21 0.2561100363398978 2201 22-23 0.27708839130828267 2201 24-25 0.1711304614664968 2201 26-27 0.24333747760448432 2201 28-29 0.3670999714754686 2201 30-31 0.33165495543764933 2201 32-33 0.36272989555953217 2201 34-35 0.2641309091957069 2201 36-37 0.27670067648413266 2201 38-39 0.2932401528183206 2201 40-41 0.2409624177042744 2201 42-43 0.244479504064806 2201 44-45 0.27285348344798876 2201 46-47 0.3159061716359375 2201 48-49 0.35016118464098156 2201 50-51 0.28458478271940635 2201 52-53 0.12654054010943838 2201 54-55 0.14939067340341694 2201 56-57 0.2562786881024195 2201 58-59 0.22170783312995468 2201 60-61 0.19953572237952955 2201 62-63 0.23764817735818866 2201 64-65 0.20299131859592734 2201 66-67 0.2888857394883928 2201 68-69 0.19529711357964885 2201 70-71 0.30766032384667596 2201 72-73 0.11296079108553414 2201 74-75 0.16179347626189156 2201 76-77 0.044710100996372404 2201 78-79 0.20157405325886657 2201 80-81 0.39158510161396975 2201 82-83 0.33886400309086895 2201 84-85 0.34932043940803936 2201 86-87 0.3349416220554815 2201 88-89 0.28666727658350766 2201 90-91 0.276856157619342 2201 92-93 0.18320226234353498 2201 94-95 0.2567171857313397 2201 96-97 0.2587775198397537 2201 98-99 0.19170476861887664 2201 100-101 0.28698819998384195 1101 1 0.06145619853764117 1101 2 0.08936607278396025 1101 3 0.0325381651040999 1101 4 0.05328689065902381 1101 5 0.07809139106746699 1101 6 0.02356497716256456 1101 7 0.054127743265937056 1101 8 -0.007012080012202659 1101 9 0.04368186162313492 1101 10-11 0.08613107483596849 1101 12-13 0.03702003546940347 1101 14-15 0.032730104639057345 1101 16-17 0.10321257825108177 1101 18-19 0.026212708057592238 1101 20-21 0.12399027505795601 1101 22-23 0.11975519136419877 1101 24-25 0.142167691004083 1101 26-27 0.13710698294299561 1101 28-29 0.14709942997814096 1101 30-31 0.12452725434368261 1101 32-33 0.10576565359962586 1101 34-35 0.10446727542569789 1101 36-37 0.058669892913549404 1101 38-39 0.009346472073723078 1101 40-41 0.027146257643558158 1101 42-43 0.06562187740338743 1101 44-45 -0.10791445308312575 1101 46-47 0.002352972378240281 1101 48-49 0.02119075217554922 1101 50-51 0.059717917696090694 1101 52-53 0.1382991668771254 1101 54-55 0.11677305689907058 1101 56-57 0.03232308265652506 1101 58-59 -0.04393071101825541 1101 60-61 0.02730037167805932 1101 62-63 -0.04033418824717927 1101 64-65 -0.08919786178400813 1101 66-67 0.0527552235292319 1101 68-69 0.02682719532180755 1101 70-71 -0.1611240435060992 1101 72-73 -0.1468850209268595 1101 74-75 -0.105920761599954 1101 76-77 -0.2441918314430538 1101 78-79 -0.17513831692835424 1101 80-81 0.03936891635704853 1101 82-83 0.13849215650700586 1101 84-85 0.18708153152342533 1101 86-87 0.14809497587935283 1101 88-89 0.11191844508500992 1101 90-91 0.04668039323477302 1101 92-93 -0.040349123327864334 1101 94-95 0.026062991266634583 1101 96-97 0.11243321619211954 1101 98-99 0.11756844304416347 1101 100-101 0.11635324402286074 2304 1 0.054556963381372725 2304 2 0.09409186455082619 2304 3 0.07994378084438125 2304 4 0.13378835143530665 2304 5 0.1028479925700907 2304 6 0.13324963672438628 2304 7 0.07688062436207588 2304 8 0.08138528414696822 2304 9 0.0803275977014124 2304 10-11 0.06447538136633568 2304 12-13 0.06614207823566431 2304 14-15 0.1254150304126469 2304 16-17 0.16098203514246023 2304 18-19 0.10321785079241153 2304 20-21 0.14479444337353442 2304 22-23 0.106050436229566 2304 24-25 0.0861366991665804 2304 26-27 0.12000172906162021 2304 28-29 0.16259559466500662 2304 30-31 0.13856327124941714 2304 32-33 0.19043848501893024 2304 34-35 0.17841387938931774 2304 36-37 0.20326265694865953 2304 38-39 0.16108689368298457 2304 40-41 0.20239357198435215 2304 42-43 0.11754417122084249 2304 44-45 0.2240246796808023 2304 46-47 0.15644747717379914 2304 48-49 0.21855262141392728 2304 50-51 0.15860739215493425 2304 52-53 0.18564881857474091 2304 54-55 0.10785759960315744 2304 56-57 0.13289485318011884 2304 58-59 0.14565874190207495 2304 60-61 0.25235843027338944 2304 62-63 0.18305734149658548 2304 64-65 0.10180958826520481 2304 66-67 0.204123298436663 2304 68-69 0.11188215621993436 2304 70-71 0.08734299807229462 2304 72-73 0.07444088092084655 2304 74-75 0.10004627601612981 2304 76-77 -0.29293282356857375 2304 78-79 0.1459485326529233 2304 80-81 0.3182406785242975 2304 82-83 0.4018755829415923 2304 84-85 0.3157604500161071 2304 86-87 0.2119418736705896 2304 88-89 0.20209870668739427 2304 90-91 0.13730092079097034 2304 92-93 0.14435942069268748 2304 94-95 0.21584413742880315 2304 96-97 0.273893482568937 2304 98-99 0.21034275279409798 2304 100-101 0.15040919483964643 2202 1 0.05905146366753655 2202 2 0.05308843723749135 2202 3 0.02040872820172268 2202 4 0.046059105643223575 2202 5 0.04532573068655665 2202 6 0.03197109383892638 2202 7 0.0629731140426486 2202 8 0.024175192596381123 2202 9 0.05021115597240566 2202 10-11 0.10548059347489414 2202 12-13 0.11987365212429069 2202 14-15 0.02213131832606763 2202 16-17 -0.0032527595900475603 2202 18-19 -3.342197280389314E-4 2202 20-21 -0.0065534303649457115 2202 22-23 0.06800956398470248 2202 24-25 -0.015926876252436273 2202 26-27 0.14285832324927838 2202 28-29 0.11698459787592697 2202 30-31 0.05249806964075532 2202 32-33 0.017495589952275736 2202 34-35 -0.008497739649321545 2202 36-37 0.004745587830363718 2202 38-39 0.11512104824804226 2202 40-41 0.10568075236219698 2202 42-43 -0.03953553621456507 2202 44-45 0.08628562836844367 2202 46-47 0.011969545575887253 2202 48-49 -0.022408650965623167 2202 50-51 0.08916180574972543 2202 52-53 0.11932183622619874 2202 54-55 0.05609844151133103 2202 56-57 0.05273845736279981 2202 58-59 0.09490771574732548 2202 60-61 -0.0060202848954560295 2202 62-63 -0.20970855542729083 2202 64-65 -0.06221520360173827 2202 66-67 0.07348870894595905 2202 68-69 -0.08114793381164986 2202 70-71 0.005009043541782887 2202 72-73 0.011622359792042403 2202 74-75 0.016809902487530337 2202 76-77 -0.3235036886339806 2202 78-79 0.07094075251345089 2202 80-81 0.290785342361616 2202 82-83 0.29061067661523765 2202 84-85 0.3957213263399595 2202 86-87 0.21619081989975086 2202 88-89 0.09189917839912454 2202 90-91 0.1255321498885209 2202 92-93 0.16638455355199966 2202 94-95 0.1641652468845365 2202 96-97 0.18917303974870237 2202 98-99 0.12213372269017242 2202 100-101 0.07052107304020439 1102 1 0.08498828379097034 1102 2 0.08424472603473987 1102 3 0.03747450103946903 1102 4 0.009328712719550936 1102 5 0.0025042408743018996 1102 6 -0.014049941807918742 1102 7 -0.012581255769262611 1102 8 -0.04412667001282955 1102 9 -0.02169651895597724 1102 10-11 0.003853682809477732 1102 12-13 0.01567852947606241 1102 14-15 -0.045468918865317676 1102 16-17 -0.13183685904494524 1102 18-19 -0.0521475384656398 1102 20-21 -0.09864852956487624 1102 22-23 -0.07708579187755049 1102 24-25 -0.08274017206423423 1102 26-27 -0.11712968495837117 1102 28-29 -0.10906975162264843 1102 30-31 -0.054014680675642524 1102 32-33 -0.08159134478416519 1102 34-35 -0.14714966641344063 1102 36-37 -0.14857586460750838 1102 38-39 -0.09171387885484705 1102 40-41 -0.08877026592181636 1102 42-43 -0.18112971736953654 1102 44-45 -0.18261963060724895 1102 46-47 -0.21687940550483376 1102 48-49 -0.19177153021565374 1102 50-51 -0.22456413668988517 1102 52-53 -0.17736733717718778 1102 54-55 -0.2138557527422691 1102 56-57 -0.23003485201680007 1102 58-59 -0.21375428024098397 1102 60-61 -0.27144983279550416 1102 62-63 -0.35973648758099586 1102 64-65 -0.2674507309980143 1102 66-67 -0.2965150263597991 1102 68-69 -0.17937089648386007 1102 70-71 -0.33697060026807435 1102 72-73 -0.34361885640133494 1102 74-75 -0.5074808132783062 1102 76-77 -0.5146615491813584 1102 78-79 -0.2743320810225356 1102 80-81 -0.14839943328705374 1102 82-83 -0.04891162011049488 1102 84-85 -0.0898203745687951 1102 86-87 -0.09436592406524369 1102 88-89 -0.1185322520822254 1102 90-91 -0.18801616559307632 1102 92-93 -0.27575784210441867 1102 94-95 -0.33090096122349877 1102 96-97 -0.2197065593931704 1102 98-99 -0.2666800015674866 1102 100-101 -0.27106135730988257 2305 1 -0.006933023409281702 2305 2 0.035661974688153464 2305 3 0.024650938029026292 2305 4 0.011455097711461804 2305 5 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1213 84-85 -0.4543963830121811 1213 86-87 -0.45816727698440474 1213 88-89 -0.6305822032209356 1213 90-91 -0.5325197870334257 1213 92-93 -0.38520182916589363 1213 94-95 -0.25422211980899334 1213 96-97 -0.41963581872292366 1213 98-99 -0.4325720311527874 1213 100-101 -0.10990054883383849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 24.0 10 30.0 11 46.0 12 83.0 13 271.0 14 921.0 15 2741.0 16 6152.0 17 10411.0 18 12843.0 19 14986.0 20 18155.0 21 22018.0 22 27381.0 23 33589.0 24 41997.0 25 52880.0 26 67791.0 27 89411.0 28 130421.0 29 181659.0 30 177117.0 31 177582.0 32 233311.0 33 331465.0 34 511951.0 35 883816.0 36 1641174.0 37 2267523.0 38 553406.0 39 361.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.26849602041696 27.35584163485429 20.312511071108435 15.063151273620315 2 31.54958364935723 25.707820119863495 28.287936683627173 14.4546595471521 3 34.95364493017705 19.997570447024398 27.078636086337237 17.970148536461316 4 30.914683066769367 19.679415726317277 28.27736179767481 21.128539409238538 5 31.808489650033735 18.24864159779548 31.223748658817264 18.719120093353528 6 29.09177867995315 18.8180986453754 33.30420707567835 18.785915598993103 7 31.84059260588169 22.02939536185037 25.86099326678878 20.269018765479164 8 29.84191997300415 28.394443745877005 22.154923926005957 19.608712355112885 9 29.43472634799711 25.529792210448022 24.744224204373957 20.29125723718091 10-11 24.499934726214512 28.72669932758657 28.922554055103888 17.850811891095027 12-13 22.66130326732868 27.955290767191027 28.576800361208527 20.806605604271763 14-15 22.7227457918431 28.535489851060973 28.455746375703093 20.286017981392835 16-17 23.65740390653621 27.530956161575453 28.93607793823992 19.875561993648418 18-19 21.360606029055244 27.336761475171535 30.797680364550757 20.504952131222467 20-21 22.69439374268673 27.80352910930516 30.42787567065811 19.074201477349998 22-23 21.28835032667978 30.64915279769013 28.742961783207804 19.31953509242228 24-25 21.371257101362605 26.93064958780061 30.64917399940199 21.048919311434798 26-27 21.099537583951566 27.153180622041823 29.145666261141596 22.601615532865015 28-29 22.34503215768438 29.36024882527209 28.5094203821333 19.785298634910237 30-31 20.182007074129928 28.759089274515915 29.979228519918916 21.07967513143524 32-33 20.0493237555733 30.21153111477214 28.678678378038537 21.060466751616026 34-35 22.088508850404498 29.93713426991204 28.799107844841142 19.17524903484232 36-37 21.69125312586681 30.534569610821404 29.04932402254175 18.724853240770035 38-39 20.365339323987698 28.644514780957188 31.3734737343449 19.61667216071022 40-41 20.571333835767952 28.04837254699379 28.12952428091381 23.25076933632445 42-43 20.833103073047106 29.68796594336905 29.796708866369208 19.682222117214632 44-45 20.281435787307863 28.133267062790452 30.011025141982323 21.57427200791936 46-47 21.981812888517442 28.070800360784194 28.612977792443367 21.334408958254993 48-49 20.83686714511814 29.70264054247846 29.45790398368352 20.002588328719884 50-51 22.08484863532218 28.3292176653427 28.884414035732664 20.701519663602458 52-53 20.540815413241948 28.180119452518305 27.789186001041056 23.489879133198695 54-55 22.396997385914588 27.982961390480394 29.467916216313267 20.152125007291755 56-57 20.91967537681003 27.616565471378884 29.481017648888024 21.98274150292306 58-59 20.656474447399724 27.832303545544306 29.777309934119877 21.733912072936086 60-61 21.837135085366306 27.955830641633046 29.563268778134372 20.643765494866276 62-63 20.144467330530006 28.1226675498066 29.610056208733383 22.12280891093001 64-65 22.045543723273163 28.209524860885786 29.56247326523629 20.182458150604763 66-67 21.09504511203318 30.758215361399543 27.876214172224294 20.270525354342986 68-69 20.929021952314816 30.526825385891588 28.03710577170041 20.507046890093182 70-71 20.64715706115736 31.2591712010936 27.906736761164304 20.186934976584737 72-73 20.77814078884557 31.42950759359705 27.7113095524151 20.081042065142288 74-75 21.60524667285422 30.675194412377515 27.404340699994695 20.315218214773573 76-77 21.663023038224125 29.701412024494473 27.690561634054223 20.94500330322718 78-79 21.301798015065053 29.631903662675192 28.28734868450254 20.778949637757215 80-81 20.80846862117265 30.194258115301427 28.15937078556895 20.83790247795698 82-83 20.15169280688223 30.19274321025821 28.29879562524144 21.35676835761812 84-85 20.381264964493678 29.95336170421798 28.445235743043135 21.220137588245212 86-87 20.232610618290682 30.125389721699015 28.895186329237237 20.746813330773065 88-89 20.235414620367507 30.484773665408028 28.793821949747212 20.485989764477253 90-91 20.790271762796458 29.637941064885975 28.526750302829335 21.045036869488232 92-93 20.39536357146023 30.044025699613343 28.060152570094832 21.5004581588316 94-95 20.218208585183152 30.428115103833903 27.67995620289455 21.673720108088393 96-97 20.46824830675636 30.806958443883914 27.37799864939669 21.346794599963033 98-99 20.649847195840426 30.05692460553459 27.699483417414665 21.593744781210315 100-101 20.899870410597828 30.222170901155295 27.25933599364835 21.61862269459853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3180.0 1 1971.0 2 905.0 3 1496.5 4 1567.0 5 1101.0 6 1111.5 7 1264.5 8 1332.0 9 1308.0 10 1228.0 11 1217.5 12 1216.5 13 1210.0 14 1288.5 15 1347.5 16 1449.0 17 1634.0 18 1809.0 19 2150.0 20 2656.0 21 3379.5 22 4590.5 23 6341.0 24 8711.5 25 11729.5 26 15539.0 27 20653.5 28 26773.5 29 33696.5 30 41944.5 31 51786.0 32 66821.5 33 89252.0 34 120404.5 35 170837.5 36 240775.5 37 325967.0 38 408138.5 39 448550.5 40 474006.5 41 469598.0 42 442223.5 43 451064.5 44 447592.0 45 433223.5 46 443974.5 47 412409.5 48 327863.5 49 258544.0 50 215144.5 51 189999.0 52 171047.5 53 145667.0 54 114546.5 55 84125.5 56 63194.5 57 52239.0 58 44610.0 59 34842.5 60 25204.0 61 18558.0 62 13285.0 63 9287.5 64 6669.5 65 4701.0 66 3343.0 67 2458.0 68 1784.0 69 1185.5 70 747.5 71 494.0 72 339.5 73 229.0 74 147.0 75 105.0 76 88.5 77 65.0 78 45.5 79 33.0 80 23.5 81 19.5 82 17.5 83 10.0 84 5.0 85 5.5 86 3.0 87 3.0 88 3.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15306635954616432 2 0.0 3 0.005766518472481293 4 0.006834392263681533 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.0067900755013464E-5 14-15 0.0 16-17 6.674211195001497E-6 18-19 0.0 20-21 0.0 22-23 1.535068574850344E-4 24-25 2.669684478000599E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010078058904452259 40-41 0.0 42-43 0.0 44-45 1.1346159031502545E-4 46-47 8.409506105701885E-4 48-49 0.002689707111585603 50-51 3.8043003811508527E-4 52-53 0.0012480774934652797 54-55 0.0024427612973705478 56-57 0.004184730419265938 58-59 0.005546269503046244 60-61 0.0017286206995053876 62-63 9.410637784952109E-4 64-65 0.0014082585621453157 66-67 0.008222628192241843 68-69 0.00744841969362167 70-71 0.009103624069982042 72-73 0.0063271522128614185 74-75 0.009070253014007034 76-77 0.0048454773275710865 78-79 0.004598531513356031 80-81 0.001988914936110446 82-83 0.0 84-85 5.205884732101167E-4 86-87 0.0015817880532153546 88-89 0.004752038370841065 90-91 0.006727604884561508 92-93 0.008943443001302004 94-95 0.008222628192241843 96-97 0.01061867001124738 98-99 0.007488464960791678 100-101 0.0074017002152566595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7491522.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.424990865098486 #Duplication Level Percentage of deduplicated Percentage of total 1 88.98935104089362 37.75372405000954 2 5.337643227136071 4.528989303048051 3 1.7381398063508338 2.212216962200945 4 0.8675028552776662 1.4721520284240734 5 0.48661232659270043 1.032226175526982 6 0.36772348144111083 0.9360399204612804 7 0.2283848530842817 0.6782457714079257 8 0.18726567604056013 0.6355795676293805 9 0.15809312684846283 0.6036389516142802 >10 1.269928829075629 10.724678763876538 >50 0.17699802413600957 5.177661087372832 >100 0.15623901393660072 14.03825716531911 >500 0.0215200677005128 6.400630331889171 >1k 0.014249536777173477 10.099382100990931 >5k 2.8488075614038023E-4 0.8298144258153806 >10k+ 6.32539526726217E-5 2.8767633944136453 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 202542 2.7036161677159862 TruSeq Adapter, Index 7 (97% over 38bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11858 0.15828559270065548 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 9861 0.1316287931878195 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9622 0.1284385202366088 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9363 0.12498127883759802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0019488696689404369 0.0 0.0 2 0.002149096004790482 0.0 0.0 3 0.0023626707630305297 0.0 0.0 4 0.0026830329003906016 0.0 0.0 5 0.0032169697959907213 0.0 1.3348422390002993E-5 6 0.003750906691590841 0.0 1.3348422390002993E-5 7 0.004578508879771026 0.0 1.3348422390002993E-5 8 0.005339368956001197 0.0 1.3348422390002993E-5 9 0.006060183765061359 0.0 2.6696844780005985E-5 10-11 0.010531905265712361 0.0 2.6696844780005985E-5 12-13 0.014389599336423226 0.0 2.6696844780005985E-5 14-15 0.020309624666389553 0.0 4.0045267170008976E-5 16-17 0.02875917603926145 0.0 4.0045267170008976E-5 18-19 0.03942456552887384 0.0 4.0045267170008976E-5 20-21 0.045538142983495206 0.0 4.0045267170008976E-5 22-23 0.05145816831346153 0.0 4.0045267170008976E-5 24-25 0.05785206263827297 0.0 4.0045267170008976E-5 26-27 0.06494674913855955 0.0 4.0045267170008976E-5 28-29 0.07328951313231144 0.0 4.0045267170008976E-5 30-31 0.12241838173871744 0.0 5.339368956001197E-5 32-33 0.13402483500682505 0.0 5.339368956001197E-5 34-35 0.1432218980335371 0.0 5.339368956001197E-5 36-37 0.1530196400677993 0.0 5.339368956001197E-5 38-39 0.16349815164395165 0.0 5.339368956001197E-5 40-41 0.17515132439042425 0.0 5.339368956001197E-5 42-43 0.18793243882885213 0.0 5.339368956001197E-5 44-45 0.20136762596439015 0.0 6.674211195001496E-5 46-47 0.21549025685301332 0.0 6.674211195001496E-5 48-49 0.22981978828868152 0.0 7.341632314501646E-5 50-51 0.24707262422776038 0.0 8.009053434001795E-5 52-53 0.2649261391743894 0.0 8.009053434001795E-5 54-55 0.28542931596543397 0.0 8.009053434001795E-5 56-57 0.3068268370566088 0.0 8.009053434001795E-5 58-59 0.33027334098464906 0.0 8.009053434001795E-5 60-61 0.3547943929150845 0.0 8.676474553501946E-5 62-63 0.3824056046288057 0.0 9.343895673002095E-5 64-65 0.41198570864505235 0.0 9.343895673002095E-5 66-67 0.44444239768634464 0.0 9.343895673002095E-5 68-69 0.4799225043989726 0.0 9.343895673002095E-5 70-71 0.5197008031211816 0.0 9.343895673002095E-5 72-73 0.5630431306215212 0.0 9.343895673002095E-5 74-75 0.610963967001632 0.0 1.0678737912002394E-4 76-77 0.6637970228212637 0.0 1.0678737912002394E-4 78-79 0.7237981814643273 0.0 1.0678737912002394E-4 80-81 0.789452396989557 0.0 1.0678737912002394E-4 82-83 0.8621278826919283 0.0 1.0678737912002394E-4 84-85 0.9439537119426467 0.0 1.0678737912002394E-4 86-87 1.0346962873498868 0.0 1.1346159031502543E-4 88-89 1.132613639791754 0.0 1.2013580151002693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 34150 0.0 64.52735 4 TCGGAAG 34230 0.0 64.45066 3 GATCGGA 34890 0.0 63.436535 1 GAAGAGC 34880 0.0 63.183315 6 ATCGGAA 35545 0.0 62.079655 2 GGAAGAG 39590 0.0 55.834396 5 ATGCCGT 25345 0.0 43.169945 48-49 GCCGTCT 25455 0.0 43.161095 50-51 TATGCCG 25315 0.0 43.12729 48-49 TGCCGTC 25450 0.0 43.01093 50-51 TCGTATG 25645 0.0 42.830914 44-45 CGTATGC 25640 0.0 42.68209 46-47 CTCGTAT 25435 0.0 42.437565 44-45 TCTCGTA 25410 0.0 42.432587 42-43 ATCTCGT 25430 0.0 42.119045 42-43 CATCTCG 25505 0.0 42.08704 40-41 CGTCTTC 26165 0.0 41.917564 52-53 GTATGCC 26420 0.0 41.493896 46-47 CGAGATT 26870 0.0 40.49712 32-33 GTCACGA 26990 0.0 40.316532 28-29 >>END_MODULE