FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491522
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2025422.7036161677159862TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA118580.15828559270065548No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC98610.1316287931878195No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG96220.1284385202366088No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC93630.12498127883759802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA341500.064.527354
TCGGAAG342300.064.450663
GATCGGA348900.063.4365351
GAAGAGC348800.063.1833156
ATCGGAA355450.062.0796552
GGAAGAG395900.055.8343965
ATGCCGT253450.043.16994548-49
GCCGTCT254550.043.16109550-51
TATGCCG253150.043.1272948-49
TGCCGTC254500.043.0109350-51
TCGTATG256450.042.83091444-45
CGTATGC256400.042.6820946-47
CTCGTAT254350.042.43756544-45
TCTCGTA254100.042.43258742-43
ATCTCGT254300.042.11904542-43
CATCTCG255050.042.0870440-41
CGTCTTC261650.041.91756452-53
GTATGCC264200.041.49389646-47
CGAGATT268700.040.4971232-33
GTCACGA269900.040.31653228-29
Produced by FastQC (version 0.11.2)