##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5928097 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69263492145962 33.0 33.0 33.0 33.0 33.0 2 32.726703864663484 33.0 33.0 33.0 33.0 33.0 3 32.76101048953821 33.0 33.0 33.0 33.0 33.0 4 36.698779051692306 37.0 37.0 37.0 37.0 37.0 5 36.698425987294065 37.0 37.0 37.0 37.0 37.0 6 36.57889622926211 37.0 37.0 37.0 37.0 37.0 7 36.50765599820651 37.0 37.0 37.0 37.0 37.0 8 36.45420545581491 37.0 37.0 37.0 37.0 37.0 9 36.481965123040325 37.0 37.0 37.0 37.0 37.0 10-11 36.41831830686981 37.0 37.0 37.0 37.0 37.0 12-13 36.41041737339993 37.0 37.0 37.0 37.0 37.0 14-15 38.29512286995304 40.0 37.0 40.0 37.0 40.0 16-17 38.28803113376856 40.0 37.0 40.0 37.0 40.0 18-19 38.28083675081565 40.0 37.0 40.0 37.0 40.0 20-21 38.254117299362676 40.0 37.0 40.0 37.0 40.0 22-23 38.12356680398449 40.0 37.0 40.0 37.0 40.0 24-25 38.12134602385892 40.0 37.0 40.0 37.0 40.0 26-27 38.05482206178475 40.0 37.0 40.0 37.0 40.0 28-29 37.906431946035966 40.0 37.0 40.0 33.0 40.0 30-31 37.889910961308495 40.0 37.0 40.0 33.0 40.0 32-33 37.751145519379996 40.0 37.0 40.0 33.0 40.0 34-35 37.62441404045852 40.0 37.0 40.0 33.0 40.0 36-37 37.41187551418271 40.0 37.0 40.0 33.0 40.0 38-39 37.45998715945437 40.0 37.0 40.0 33.0 40.0 40-41 37.33398491961248 40.0 37.0 40.0 33.0 40.0 42-43 37.179636821057414 40.0 37.0 40.0 33.0 40.0 44-45 37.125314919779484 40.0 37.0 40.0 33.0 40.0 46-47 37.0928098848585 40.0 37.0 40.0 33.0 40.0 48-49 36.862099759838614 37.0 37.0 40.0 33.0 40.0 50-51 36.75856130559267 37.0 37.0 40.0 33.0 40.0 52-53 36.634371957813784 37.0 37.0 40.0 33.0 40.0 54-55 36.41133090433574 37.0 37.0 40.0 33.0 40.0 56-57 36.27087680920201 37.0 37.0 40.0 33.0 40.0 58-59 36.03785540283838 37.0 37.0 40.0 33.0 40.0 60-61 35.77295361395065 37.0 37.0 40.0 27.0 40.0 62-63 35.531476121257796 37.0 35.0 40.0 27.0 40.0 64-65 35.25628890687855 37.0 33.0 38.5 27.0 40.0 66-67 34.83405872069907 37.0 33.0 37.0 27.0 40.0 68-69 34.60852209402107 37.0 33.0 37.0 27.0 40.0 70-71 34.1882819225124 37.0 33.0 37.0 27.0 40.0 72-73 33.64137243368319 37.0 33.0 37.0 27.0 40.0 74-75 33.10685688847534 37.0 33.0 37.0 22.0 37.0 76-77 30.451836314419282 33.0 30.0 35.0 22.0 37.0 78-79 32.760648653353684 35.0 33.0 37.0 24.5 37.0 80-81 33.094020897431335 37.0 33.0 37.0 27.0 37.0 82-83 33.013906823724376 37.0 33.0 37.0 27.0 37.0 84-85 32.82994390948731 37.0 33.0 37.0 24.5 37.0 86-87 32.656627497829405 37.0 33.0 37.0 22.0 37.0 88-89 32.501188745730715 37.0 33.0 37.0 22.0 37.0 90-91 32.347656170268465 35.0 33.0 37.0 22.0 37.0 92-93 32.22438423325394 33.0 33.0 37.0 22.0 37.0 94-95 32.046199733236485 33.0 33.0 37.0 22.0 37.0 96-97 31.885621136091395 33.0 33.0 37.0 22.0 37.0 98-99 31.75459654253296 33.0 33.0 37.0 22.0 37.0 100-101 30.195452520429406 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11400864189875648 2306 2 0.118667143977909 2306 3 0.10891153422599587 2306 4 0.1643996098525733 2306 5 0.12635359212406883 2306 6 0.11763400146774217 2306 7 0.14860295974673932 2306 8 0.18475149091251808 2306 9 0.17914806217599732 2306 10-11 0.18796362495093888 2306 12-13 0.1968238601583252 2306 14-15 0.44863684999316433 2306 16-17 0.3222173801522885 2306 18-19 0.347737850851054 2306 20-21 0.25467812049343763 2306 22-23 0.3427333786851463 2306 24-25 0.3625492227508076 2306 26-27 0.365628654060977 2306 28-29 0.4041188264801079 2306 30-31 0.383074286574292 2306 32-33 0.42891085396654915 2306 34-35 0.44989935640422374 2306 36-37 0.35412004756469173 2306 38-39 0.3852905984603865 2306 40-41 0.3926221547511588 2306 42-43 0.4756920143588488 2306 44-45 0.522060686443794 2306 46-47 0.4421367434686445 2306 48-49 0.37709433961907735 2306 50-51 0.32207321830211555 2306 52-53 0.34239417241089853 2306 54-55 0.2819683156994941 2306 56-57 0.4125030641068719 2306 58-59 0.2813799506504395 2306 60-61 0.3997651967789082 2306 62-63 0.37404573202492486 2306 64-65 0.299580195846481 2306 66-67 0.2180189299397668 2306 68-69 0.2346883762931924 2306 70-71 0.30896257704207386 2306 72-73 0.19442951242304218 2306 74-75 0.22531433730517136 2306 76-77 -0.06752770368778371 2306 78-79 0.15963886660474458 2306 80-81 0.28941069018119236 2306 82-83 0.35446533054668095 2306 84-85 0.3704865163023854 2306 86-87 0.3509610701604515 2306 88-89 0.32023189663138396 2306 90-91 0.3378205452427352 2306 92-93 0.24022344235221738 2306 94-95 0.22349307306094346 2306 96-97 0.42039129911970363 2306 98-99 0.37557731129817284 2306 100-101 0.1167796204277316 2307 1 0.1447337517686904 2307 2 0.12016716930815363 2307 3 0.12277762589614127 2307 4 0.16117839450750893 2307 5 0.15705020547057558 2307 6 0.1694647447837312 2307 7 0.20451139725069822 2307 8 0.1619393906554123 2307 9 0.18299271812797002 2307 10-11 0.2127293202027829 2307 12-13 0.2041048802072183 2307 14-15 0.37594744310080586 2307 16-17 0.37961531510423185 2307 18-19 0.25019559683937587 2307 20-21 0.3355187204402412 2307 22-23 0.3735131068916431 2307 24-25 0.37604066429494054 2307 26-27 0.4338135521700721 2307 28-29 0.4609757232720355 2307 30-31 0.4922159712887648 2307 32-33 0.47840717791502385 2307 34-35 0.5181411684031616 2307 36-37 0.4545807413600471 2307 38-39 0.46595602394514657 2307 40-41 0.4514464990144873 2307 42-43 0.3445742065647366 2307 44-45 0.4147533071107361 2307 46-47 0.3071394506383669 2307 48-49 0.42374101122513963 2307 50-51 0.34571615053105376 2307 52-53 0.32249505010381796 2307 54-55 0.41587268769944075 2307 56-57 0.33254712289836164 2307 58-59 0.34937487193670336 2307 60-61 0.4246617385674796 2307 62-63 0.43701790358665704 2307 64-65 0.37669732922287835 2307 66-67 0.34742607560133365 2307 68-69 0.244876437516389 2307 70-71 0.2879984763290224 2307 72-73 0.28203724511298844 2307 74-75 0.20629702408404427 2307 76-77 -0.13455754271408793 2307 78-79 0.10819211394190376 2307 80-81 0.36234310909275536 2307 82-83 0.32214172402667174 2307 84-85 0.37237931871381846 2307 86-87 0.28630879107183205 2307 88-89 0.2663296935334998 2307 90-91 0.3791914813218611 2307 92-93 0.35888969423615436 2307 94-95 0.3542837249347528 2307 96-97 0.3560978387552076 2307 98-99 0.2606139451621772 2307 100-101 0.2692591345936677 2201 1 0.13745309693662477 2201 2 0.1272974738769861 2201 3 0.1110215952639777 2201 4 0.10867782030550188 2201 5 0.11935875443187172 2201 6 0.09341773274966414 2201 7 0.10851199865174976 2201 8 0.146865832355779 2201 9 0.1276868648940095 2201 10-11 0.1804727667445789 2201 12-13 0.13850721146737754 2201 14-15 0.2190338997908583 2201 16-17 0.23224693580451827 2201 18-19 0.14942786608571623 2201 20-21 0.20119681523797084 2201 22-23 0.31487839305578547 2201 24-25 0.25755880152317445 2201 26-27 0.21339628863552917 2201 28-29 0.1811130633265563 2201 30-31 0.2099794393480181 2201 32-33 0.1815845927366908 2201 34-35 0.20467163586403814 2201 36-37 0.1906593647258319 2201 38-39 0.20710651322570328 2201 40-41 0.19899650747641573 2201 42-43 0.2149029598815062 2201 44-45 0.1252975070660156 2201 46-47 0.13279302096602663 2201 48-49 0.21881379132994994 2201 50-51 0.14877619392863295 2201 52-53 0.08780244461686237 2201 54-55 0.09115993981038883 2201 56-57 0.2413156786896451 2201 58-59 0.19326974461496604 2201 60-61 0.14299619763896487 2201 62-63 0.1352069384437442 2201 64-65 0.08422640197729692 2201 66-67 0.23207569149041518 2201 68-69 0.13957402193919677 2201 70-71 0.1192956591126304 2201 72-73 0.08473784560740683 2201 74-75 0.0643070665749903 2201 76-77 -0.08917858449786209 2201 78-79 0.11745244353385687 2201 80-81 0.32021103658615147 2201 82-83 0.41936923495162404 2201 84-85 0.41011194608089596 2201 86-87 0.31956377605191477 2201 88-89 0.3079942824955211 2201 90-91 0.27513549964798045 2201 92-93 0.18789139115420284 2201 94-95 0.2506255292587909 2201 96-97 0.33425606314125744 2201 98-99 0.18138388961568808 2201 100-101 0.20056833028775856 1101 1 0.08964469844718082 1101 2 0.10410826483690272 1101 3 0.09105941979004228 1101 4 0.08839633373344213 1101 5 0.09955707835239735 1101 6 0.13422508352066842 1101 7 0.11037605313310905 1101 8 0.07021260420833642 1101 9 0.08279158948312215 1101 10-11 0.12368855353928154 1101 12-13 0.08007142405161005 1101 14-15 0.10795186384169853 1101 16-17 0.10151891325156015 1101 18-19 0.05323202239559066 1101 20-21 0.08605436400886646 1101 22-23 0.1361712620896327 1101 24-25 0.15059068065210823 1101 26-27 0.17913920337203848 1101 28-29 0.1172767497563072 1101 30-31 0.09913806810599368 1101 32-33 0.16608931287030515 1101 34-35 0.11696242971328275 1101 36-37 0.0312657452771532 1101 38-39 0.06934554559830985 1101 40-41 0.029672431014567735 1101 42-43 0.08117253540701341 1101 44-45 -0.10916358626058553 1101 46-47 -0.005668591494263353 1101 48-49 -0.008621276454462645 1101 50-51 0.0027141503646106457 1101 52-53 0.11191152952390837 1101 54-55 0.036993419410258355 1101 56-57 -0.1066089510647501 1101 58-59 -0.038209030310540015 1101 60-61 -0.019567748965201304 1101 62-63 -0.060040406143563985 1101 64-65 -0.1365564637756762 1101 66-67 -0.13270247168093618 1101 68-69 -0.17434971401414856 1101 70-71 -0.2523877753246282 1101 72-73 -0.24401917349860724 1101 74-75 -0.21932160702155556 1101 76-77 -0.26680778735424227 1101 78-79 -0.17154789670460957 1101 80-81 -0.02912239305807418 1101 82-83 0.01651119810055235 1101 84-85 -0.07389633980582744 1101 86-87 -0.0256013104861168 1101 88-89 -0.04330842915102551 1101 90-91 -0.13938718089105606 1101 92-93 -0.16363038275734 1101 94-95 -0.052168139065415176 1101 96-97 -0.15282390437406335 1101 98-99 -0.012844427270536585 1101 100-101 -0.04757461914767802 2304 1 0.05368092267822533 2304 2 0.0871321388268882 2304 3 0.07313221953030791 2304 4 0.09450138871760316 2304 5 0.08459707616260914 2304 6 0.08582047189214137 2304 7 0.0644067040546048 2304 8 0.0747974306639918 2304 9 0.055460398794579646 2304 10-11 0.005564690726330923 2304 12-13 0.03232800614539855 2304 14-15 0.07575942422999304 2304 16-17 0.05716850414935237 2304 18-19 0.0869923449936536 2304 20-21 0.07528624333948386 2304 22-23 0.007890308741956176 2304 24-25 0.11091012329074346 2304 26-27 0.10130964627906991 2304 28-29 0.05628712234688038 2304 30-31 0.02763550993410746 2304 32-33 0.08166304441014205 2304 34-35 0.14534665656294976 2304 36-37 0.077785563220786 2304 38-39 0.15177840546645172 2304 40-41 0.06036399281101268 2304 42-43 0.0228492195843657 2304 44-45 0.06371116178095804 2304 46-47 0.08282547061202905 2304 48-49 0.11336761570390053 2304 50-51 0.03352594129783171 2304 52-53 0.05945933034766426 2304 54-55 0.013556341162669128 2304 56-57 0.024337667578691935 2304 58-59 0.07575342170235189 2304 60-61 0.05983486144221217 2304 62-63 0.0539248591360888 2304 64-65 -0.034951799227322056 2304 66-67 0.043627405136000164 2304 68-69 -0.02637256686433176 2304 70-71 0.011655539098988754 2304 72-73 -0.0638579314945531 2304 74-75 -0.06552388698417388 2304 76-77 -0.35224510206831283 2304 78-79 0.03371730310956167 2304 80-81 0.10989465153677003 2304 82-83 0.15888849975194574 2304 84-85 0.27344692892121003 2304 86-87 0.17668498198565885 2304 88-89 0.1620820370681031 2304 90-91 0.09048716492937814 2304 92-93 0.13574020583128998 2304 94-95 0.12740315329610752 2304 96-97 0.0985137089264505 2304 98-99 0.08464781153475087 2304 100-101 0.13491256718258526 2202 1 0.03231676525594196 2202 2 0.04793286129881835 2202 3 0.06077411979709524 2202 4 0.04653566990720037 2202 5 0.03835007460225626 2202 6 0.04076844718525763 2202 7 0.04979269089381688 2202 8 0.029159561956191737 2202 9 0.0686439589354535 2202 10-11 0.046926726275472674 2202 12-13 0.10732250738875848 2202 14-15 0.03771423868253976 2202 16-17 -0.018554420619885548 2202 18-19 -0.04169770885535229 2202 20-21 -0.04416745195435823 2202 22-23 -0.06992809514365916 2202 24-25 -0.06576169279978217 2202 26-27 0.01766230159630311 2202 28-29 0.019174525924562147 2202 30-31 0.026141141954717284 2202 32-33 -0.012270468506116572 2202 34-35 -0.06571606548978792 2202 36-37 -0.07360258171868139 2202 38-39 -0.016486135186823958 2202 40-41 -0.007502049714425141 2202 42-43 -0.05640248373637036 2202 44-45 -0.03357232305929614 2202 46-47 -0.06295756527091356 2202 48-49 -0.26119112524903443 2202 50-51 0.004455761637281341 2202 52-53 0.006964447425900744 2202 54-55 -0.05581519623793696 2202 56-57 -0.05477275004216864 2202 58-59 -0.1283128117916732 2202 60-61 -0.061883545289816766 2202 62-63 -0.21336853640435294 2202 64-65 -0.22548824498630182 2202 66-67 -0.15263447541289565 2202 68-69 -0.15861934588676974 2202 70-71 -0.12221499041186945 2202 72-73 -0.06661714288228637 2202 74-75 -0.057231244449937435 2202 76-77 -0.5685190928200505 2202 78-79 -0.16994939719655378 2202 80-81 -0.015773803196175606 2202 82-83 0.07397217142054302 2202 84-85 0.18427417480749853 2202 86-87 0.08142710970689393 2202 88-89 0.08590653228223744 2202 90-91 0.02832740858251981 2202 92-93 0.054764351195125016 2202 94-95 0.011213519940262984 2202 96-97 -0.017172367704223035 2202 98-99 -0.09099981261423551 2202 100-101 -0.08558860890441977 1102 1 0.08720625438874663 1102 2 0.06975568275384347 1102 3 0.03704828602653265 1102 4 -0.0186203284743911 1102 5 0.03252321121694024 1102 6 -0.02563479255204726 1102 7 0.004696414623204248 1102 8 0.008932026432496798 1102 9 0.04675058064543691 1102 10-11 0.04724573290600631 1102 12-13 0.018733503480326874 1102 14-15 -0.03687201404648732 1102 16-17 -0.11594977382194571 1102 18-19 -0.010419055015304934 1102 20-21 -0.09311899729280526 1102 22-23 -0.0753364203130289 1102 24-25 -0.022814524639272804 1102 26-27 -0.06267804587004377 1102 28-29 -0.08680989754031287 1102 30-31 -0.11131302019550304 1102 32-33 -0.107022183806599 1102 34-35 -0.18672539791879927 1102 36-37 -0.11206455646641444 1102 38-39 -0.0983110357460788 1102 40-41 -0.08505379073513808 1102 42-43 -0.13840333402047378 1102 44-45 -0.12658719639587446 1102 46-47 -0.13929086590101747 1102 48-49 -0.147194946313995 1102 50-51 -0.15023584922455058 1102 52-53 -0.1750933570431954 1102 54-55 -0.24756219516314815 1102 56-57 -0.209677943047339 1102 58-59 -0.18949716814382356 1102 60-61 -0.17127848962243775 1102 62-63 -0.19784201984711558 1102 64-65 -0.15503146294356185 1102 66-67 -0.2194647846448916 1102 68-69 -0.0282069170143302 1102 70-71 -0.14469579463971627 1102 72-73 -0.14088415200080107 1102 74-75 -0.20015811132675765 1102 76-77 -0.3603673453850824 1102 78-79 -0.032854875841586306 1102 80-81 0.0636086712506696 1102 82-83 0.07317158990330341 1102 84-85 0.03664366638594174 1102 86-87 0.12810190952597367 1102 88-89 0.09015247072211707 1102 90-91 0.150099200351697 1102 92-93 0.095447918324993 1102 94-95 -0.05769134350477145 1102 96-97 0.07559356607820433 1102 98-99 -0.0546626792281657 1102 100-101 -0.1320307733909516 2305 1 -9.986675208537577E-4 2305 2 0.04617884743746714 2305 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0.06924366886831024 1213 68-69 0.03504426390185955 1213 70-71 -0.13104391703819118 1213 72-73 0.10558663399274337 1213 74-75 0.2287839519582846 1213 76-77 0.34942926638197136 1213 78-79 -0.16562495004429678 1213 80-81 -0.28439264470488723 1213 82-83 -0.20412620038342055 1213 84-85 -0.15905708797221507 1213 86-87 -0.20240662946817878 1213 88-89 -0.38288873627121234 1213 90-91 -0.14345501393875537 1213 92-93 -0.07376169246643371 1213 94-95 0.09092689049064262 1213 96-97 -0.0157998155715795 1213 98-99 -0.1618345103299461 1213 100-101 0.1928426721993617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 28.0 10 25.0 11 33.0 12 65.0 13 153.0 14 440.0 15 1098.0 16 2031.0 17 3538.0 18 5638.0 19 7940.0 20 10901.0 21 14507.0 22 18820.0 23 24158.0 24 31261.0 25 39921.0 26 52298.0 27 73230.0 28 113529.0 29 159023.0 30 136823.0 31 129985.0 32 170288.0 33 240858.0 34 366607.0 35 625227.0 36 1196626.0 37 1913542.0 38 588871.0 39 628.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.251785643084936 25.19574662486594 22.10662970469393 15.445838027355194 2 28.825422391030376 25.863004603332236 31.65538620572504 13.656186799912351 3 35.919951793066474 19.3999842774139 28.246570637429404 16.433493292090223 4 27.9897645761414 19.81107518343276 32.042011934242396 20.157148306183448 5 34.76203240264118 18.35889325022853 30.26279765665103 16.616276690479253 6 28.97035929067962 19.467360267552976 34.77345259364009 16.788827848127315 7 31.085574341985296 23.197697338623172 27.507832614749727 18.208895704641808 8 27.700288979751846 29.31112631928931 25.364261077374405 17.62432362358443 9 32.131559250801736 23.548383233270307 25.76906214591293 18.550995370015034 10-11 24.50696235233668 30.084080945369145 29.1687113081989 16.240245394095272 12-13 22.65690860930392 28.516575947490697 29.11745219899993 19.70906324420545 14-15 21.859848109772834 28.84977253239952 29.66854287303329 19.62183648479436 16-17 23.154785014042872 27.289248749577546 30.33217323638372 19.22379299999587 18-19 20.657792880244706 27.41499506502677 32.41093220977997 19.516279844948556 20-21 22.79422047243829 27.696316372690934 31.93825101040013 17.571212144470646 22-23 20.822112031119662 31.674200015688037 29.6920928090606 17.811595144131704 24-25 20.6914851489825 27.381719812037442 31.79467166141301 20.132123377567048 26-27 20.64610278812914 27.663017322422355 29.470435453400984 22.220444436047522 28-29 22.319970472817836 29.67636157100668 29.412398278908054 18.591269677267427 30-31 19.949327752228076 27.997188642493537 31.40349255418729 20.649991051091103 32-33 19.940775260593746 30.090980292663904 29.702094955598735 20.26614949114362 34-35 22.309216600200703 29.911833426477337 29.71346454013826 18.0654854331837 36-37 20.223682237318314 29.71025946437786 31.864002900087495 18.202055398216324 38-39 20.787029699805274 27.49550841927313 31.19684421592954 20.520617664992052 40-41 22.57186412435559 27.707694391640352 30.781007800648336 18.939433683355723 42-43 20.37972725480032 29.846002857240695 31.1650855240729 18.609184363886083 44-45 20.15234079169338 27.776499026119794 31.49256071579879 20.578599466388038 46-47 22.297873122859958 27.66484638920762 29.3544031630048 20.682877324927624 48-49 20.3441494973446 30.047834669777824 30.793104762920137 18.814911069957446 50-51 22.705557100933135 27.88013069339733 30.32749428018177 19.086817925487757 52-53 20.566227592588064 27.737594688263588 28.35177264526599 23.34440507388236 54-55 22.675472542213683 27.936873812402833 30.17977083423303 19.20788281115046 56-57 20.49327466976438 28.209020763644197 30.290462941391706 21.007241625199715 58-59 20.483038781958093 28.17422008897652 30.617704696567927 20.72503643249746 60-61 22.28837815805422 28.43599377361411 30.476412987691038 18.79921508064064 62-63 20.100284487162735 28.305717804827584 30.45983783329233 21.134159874717348 64-65 22.14649224827942 28.028925069498488 30.390697108716058 19.433885573506036 66-67 20.337415972236222 31.406622214008262 28.57541598556375 19.680545828191757 68-69 20.177808114074676 31.80931961631857 28.541249059910324 19.47162320969643 70-71 19.907281386289963 32.50817439278199 28.251180576919005 19.333363644009044 72-73 19.94499380071058 32.252424234679395 28.1125101652067 19.690071799403327 74-75 20.18045421015539 31.59136353725005 28.355612802438785 19.872569450155776 76-77 20.088473144491097 31.26660663864891 28.508292512172545 20.136627704687456 78-79 19.906398663922534 31.182133337831914 28.68694119234792 20.224526805897636 80-81 19.79610203501539 31.246928752109703 28.4512222997268 20.505746913148105 82-83 19.519442748659475 31.00365935307739 28.596124523603443 20.880773374659693 84-85 19.661164280516854 30.75856377743528 28.833694370228258 20.746577571819603 86-87 19.981049256161374 30.596232337738893 29.11944408902194 20.303274317077793 88-89 20.208771067101793 30.701754533944804 28.723789746085593 20.365684652867806 90-91 20.271700118511895 30.527390587371944 28.600547432171975 20.600361861944187 92-93 19.847030693565564 31.007524383263245 28.699201640346256 20.446243282824934 94-95 19.544725135906972 31.15917884925798 28.708230874501318 20.587865140333736 96-97 19.749298010134567 31.298600271685768 28.562594349394914 20.38950736878475 98-99 20.274163628351847 30.760145542196643 28.175680052796835 20.790010776654675 100-101 20.429851390859415 30.685628151554667 27.635935441314658 21.24858501627126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 87.0 2 47.0 3 83.0 4 110.0 5 89.0 6 64.0 7 60.0 8 63.5 9 65.0 10 62.5 11 64.5 12 74.0 13 85.0 14 91.5 15 103.5 16 135.0 17 184.5 18 284.5 19 491.5 20 803.0 21 1290.5 22 2115.5 23 3418.0 24 5274.5 25 8180.5 26 13175.0 27 19851.0 28 27580.0 29 36947.0 30 47699.5 31 59476.0 32 76981.0 33 105359.5 34 142151.0 35 189471.5 36 257320.0 37 329469.5 38 389817.5 39 420461.0 40 426950.5 41 415344.0 42 385846.0 43 358347.0 44 323134.5 45 290539.5 46 271308.0 47 244094.5 48 209062.0 49 180303.5 50 150683.5 51 122956.5 52 99849.5 53 78329.0 54 62352.5 55 49336.5 56 35978.5 57 24683.0 58 17447.0 59 12721.0 60 9185.5 61 6471.5 62 4442.5 63 2952.5 64 1998.5 65 1423.0 66 989.0 67 671.0 68 445.0 69 316.5 70 227.5 71 151.5 72 110.0 73 85.0 74 59.0 75 39.5 76 30.0 77 23.0 78 14.0 79 9.0 80 7.0 81 7.0 82 4.0 83 1.5 84 2.0 85 1.5 86 1.5 87 3.0 88 1.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15554738729814982 2 0.0 3 0.005381153513513696 4 0.007557231266627385 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.590968906210542E-5 14-15 0.0 16-17 8.434409895789493E-6 18-19 0.0 20-21 0.0 22-23 2.024258374989478E-4 24-25 2.5303229687368475E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010374324171821075 40-41 0.0 42-43 0.0 44-45 1.0964732864526339E-4 46-47 0.001062735646869476 48-49 0.003019518742692638 50-51 4.048516749978956E-4 52-53 0.0015519314208252667 54-55 0.0027158799864442163 56-57 0.004790744820808431 58-59 0.005963127796323171 60-61 0.001678447569262109 62-63 0.0010374324171821075 64-65 0.0015266281911378982 66-67 0.008729614242142124 68-69 0.007945214121833702 70-71 0.009994775726510547 72-73 0.006772831146318961 74-75 0.009353760574430546 76-77 0.005220899725493696 78-79 0.005010039478098958 80-81 0.0022266842124884258 82-83 0.0 84-85 5.482366432263171E-4 86-87 0.001644709929678951 88-89 0.005263071774972643 90-91 0.007118641952046331 92-93 0.009581489641616862 94-95 0.00865370455308002 96-97 0.011150289882233709 98-99 0.007911476482250544 100-101 0.007877738842667384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5928097.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.04920640875416 #Duplication Level Percentage of deduplicated Percentage of total 1 91.65949506634922 50.45782463229641 2 4.029297092724478 4.436192146791655 3 1.2892438161042983 2.129155468318062 4 0.6330947973875632 1.3940546471078545 5 0.36262803207291244 0.9981192693591039 6 0.28179696207796423 0.9307619478473833 7 0.23877321222204548 0.920099309114485 8 0.1755263758600156 0.7730070155918849 9 0.10562422569014777 0.52330768216036 >10 0.9911744393631423 11.149854175139595 >50 0.12702548998470314 4.877296166465092 >100 0.08968005782183447 9.769514464980608 >500 0.013476488219847768 5.114378964522642 >1k 0.003040872848016834 2.340871347195874 >5k 0.0 0.0 >10k+ 1.2307127360451457E-4 4.185562763109005 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 214738 3.6223766244040876 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 12073 0.20365726134373308 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10221 0.1724162070897288 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10087 0.1701557852376572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.916216114547383E-4 0.0 0.0 2 8.265721697873702E-4 0.0 0.0 3 8.603098093705281E-4 0.0 1.6868819791578983E-5 4 9.109162687452651E-4 0.0 1.6868819791578983E-5 5 9.78391547911581E-4 0.0 3.3737639583157966E-5 6 0.001012129187494739 0.0 3.3737639583157966E-5 7 0.0012482926645768447 0.0 3.3737639583157966E-5 8 0.0014001120427010556 0.0 5.060645937473695E-5 9 0.0016868819791578983 0.0 5.060645937473695E-5 10-11 0.003036387562484217 0.0 6.747527916631593E-5 12-13 0.004242508177582115 0.0 8.434409895789492E-5 14-15 0.005659489040074749 0.0 1.012129187494739E-4 16-17 0.006916216114547383 0.0 1.1808173854105288E-4 18-19 0.008619966913496861 0.0 1.1808173854105288E-4 20-21 0.01045866827077897 0.0 1.1808173854105288E-4 22-23 0.012921515960349503 0.0 1.2651614843684237E-4 24-25 0.01558678948741898 0.0 1.3495055833263186E-4 26-27 0.01874969319834004 0.0 1.5181937812421085E-4 28-29 0.022148760386343204 0.0 1.6025378802000034E-4 30-31 0.03193267586545902 0.0 1.6868819791578983E-4 32-33 0.0356353818097106 0.0 1.8555701770736881E-4 34-35 0.039000711358130616 0.0 1.8555701770736881E-4 36-37 0.04257690115394536 0.0 1.8555701770736881E-4 38-39 0.04686158138100642 0.0 2.1929465729052678E-4 40-41 0.051736670300772745 0.0 2.1929465729052678E-4 42-43 0.055877965559605385 0.0 2.1929465729052678E-4 44-45 0.06093017708718329 0.0 2.1929465729052678E-4 46-47 0.06646314997882119 0.0 2.1929465729052678E-4 48-49 0.07272148212149701 0.0 2.1929465729052678E-4 50-51 0.07897137985427702 0.0 2.1929465729052678E-4 52-53 0.08502728615945387 0.0 2.1929465729052678E-4 54-55 0.0914964785495244 0.0 2.3616347708210576E-4 56-57 0.09852234199271706 0.0 2.3616347708210576E-4 58-59 0.10657720344319602 0.0 2.3616347708210576E-4 60-61 0.11474171222232024 0.0 2.5303229687368475E-4 62-63 0.12372435876133606 0.0 2.5303229687368475E-4 64-65 0.13365165920868027 0.0 2.6146670676947424E-4 66-67 0.14357895965602452 0.0 2.6990111666526373E-4 68-69 0.15463647102940453 0.0 2.6990111666526373E-4 70-71 0.16706035680590248 0.0 2.867699364568427E-4 72-73 0.1811542557417667 0.0 3.120731661442112E-4 74-75 0.19717963454376675 0.0 3.2894198593579017E-4 76-77 0.2147063383072173 0.0 3.3737639583157966E-4 78-79 0.23538751137169314 0.0 3.3737639583157966E-4 80-81 0.25863274504448897 0.0 3.3737639583157966E-4 82-83 0.28411309733966905 0.0 3.6267962551894814E-4 84-85 0.31250332104889644 0.0 4.048516749978956E-4 86-87 0.34596262510549336 0.0 4.048516749978956E-4 88-89 0.38451631273914716 0.0 4.048516749978956E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28005 0.0 82.890175 3 GATCGGA 28275 0.0 82.30178 1 CGGAAGA 28155 0.0 82.22508 4 ATCGGAA 28575 0.0 81.2201 2 GAAGAGC 29950 0.0 77.42133 6 GGAAGAG 33405 0.0 69.541794 5 AGAGCAC 34110 0.0 67.99307 8 GAGCACA 34205 0.0 67.67925 9 AAGAGCA 36010 0.0 64.379166 7 GCCGTCT 25045 0.0 45.74401 50-51 TATGCCG 24990 0.0 45.568897 48-49 CGTATGC 25110 0.0 45.445515 46-47 TCGTATG 25360 0.0 45.43765 44-45 TGCCGTC 25075 0.0 45.386208 50-51 ATGCCGT 25190 0.0 45.376797 48-49 TTGTGTT 61715 0.0 45.333138 1 TCTCGTA 25195 0.0 45.292194 42-43 TCGTATC 24735 0.0 45.269997 38-39 CTCGTAT 25115 0.0 45.22843 44-45 CGTATCT 24910 0.0 45.190308 38-39 >>END_MODULE