FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5928097
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2147383.6223766244040876TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC120730.20365726134373308No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA102210.1724162070897288No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG100870.1701557852376572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG280050.082.8901753
GATCGGA282750.082.301781
CGGAAGA281550.082.225084
ATCGGAA285750.081.22012
GAAGAGC299500.077.421336
GGAAGAG334050.069.5417945
AGAGCAC341100.067.993078
GAGCACA342050.067.679259
AAGAGCA360100.064.3791667
GCCGTCT250450.045.7440150-51
TATGCCG249900.045.56889748-49
CGTATGC251100.045.44551546-47
TCGTATG253600.045.4376544-45
TGCCGTC250750.045.38620850-51
ATGCCGT251900.045.37679748-49
TTGTGTT617150.045.3331381
TCTCGTA251950.045.29219442-43
TCGTATC247350.045.26999738-39
CTCGTAT251150.045.2284344-45
CGTATCT249100.045.19030838-39
Produced by FastQC (version 0.11.2)