FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6731577
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT745581.1075859341726315TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA152400.053.737814
TCGGAAG152950.053.577623
GATCGGA158800.051.9802671
ATCGGAA159900.051.2488822
GAAGAGC175950.046.7276736
GCCGTCT96800.042.0521350-51
TATGCCG96250.041.7987848-49
ATGCCGT98550.041.23293748-49
TGCCGTC98000.041.12522550-51
TCTCGTA97700.041.05673242-43
CGTATGC98100.041.0345846-47
TCGTATG100750.040.56814244-45
CTCGTAT98450.040.5268544-45
CCGTCTT100250.040.34465452-53
CATCTCG100200.039.88896640-41
ATCTCGT101550.038.98566442-43
CGTCTTC105250.038.74395452-53
GTATGCC105250.038.65312246-47
CGAGATT105300.038.3184432-33
AGAGCAC214800.038.2320068
Produced by FastQC (version 0.11.2)