##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6731577 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69480301569751 33.0 33.0 33.0 33.0 33.0 2 32.730098311287236 33.0 33.0 33.0 33.0 33.0 3 32.76189591235456 33.0 33.0 33.0 33.0 33.0 4 36.70319124330005 37.0 37.0 37.0 37.0 37.0 5 36.69964764571511 37.0 37.0 37.0 37.0 37.0 6 36.58732998226122 37.0 37.0 37.0 37.0 37.0 7 36.53550096804954 37.0 37.0 37.0 37.0 37.0 8 36.481796316078686 37.0 37.0 37.0 37.0 37.0 9 36.48840249469032 37.0 37.0 37.0 37.0 37.0 10-11 36.43966242976943 37.0 37.0 37.0 37.0 37.0 12-13 36.43440875741301 37.0 37.0 37.0 37.0 37.0 14-15 38.34756625379165 40.0 37.0 40.0 37.0 40.0 16-17 38.32593269006654 40.0 37.0 40.0 37.0 40.0 18-19 38.3335583177612 40.0 37.0 40.0 37.0 40.0 20-21 38.27857350513854 40.0 37.0 40.0 37.0 40.0 22-23 38.17694620443322 40.0 37.0 40.0 37.0 40.0 24-25 38.153267429014036 40.0 37.0 40.0 37.0 40.0 26-27 38.082656411714524 40.0 37.0 40.0 37.0 40.0 28-29 37.957376035362884 40.0 37.0 40.0 33.0 40.0 30-31 37.91779088317641 40.0 37.0 40.0 33.0 40.0 32-33 37.786031638648716 40.0 37.0 40.0 33.0 40.0 34-35 37.70688398869982 40.0 37.0 40.0 33.0 40.0 36-37 37.60169964630873 40.0 37.0 40.0 33.0 40.0 38-39 37.53079568130915 40.0 37.0 40.0 33.0 40.0 40-41 37.49384156194009 40.0 37.0 40.0 33.0 40.0 42-43 37.36199905311935 40.0 37.0 40.0 33.0 40.0 44-45 37.241341367706255 40.0 37.0 40.0 33.0 40.0 46-47 37.190971001297314 40.0 37.0 40.0 33.0 40.0 48-49 37.01399077808959 38.5 37.0 40.0 33.0 40.0 50-51 36.88892149640419 37.0 37.0 40.0 33.0 40.0 52-53 36.75372457300867 37.0 37.0 40.0 33.0 40.0 54-55 36.589835487286265 37.0 37.0 40.0 33.0 40.0 56-57 36.42873861503776 37.0 37.0 40.0 33.0 40.0 58-59 36.23800106572353 37.0 37.0 40.0 33.0 40.0 60-61 35.98655173965922 37.0 37.0 40.0 33.0 40.0 62-63 35.75195663660982 37.0 37.0 40.0 27.0 40.0 64-65 35.516266842078764 37.0 33.0 40.0 27.0 40.0 66-67 35.22988617674581 37.0 33.0 38.5 27.0 40.0 68-69 34.966931374327295 37.0 33.0 37.0 27.0 40.0 70-71 34.6123851810653 37.0 33.0 37.0 27.0 40.0 72-73 34.27340228003037 37.0 33.0 37.0 27.0 40.0 74-75 33.904773873937714 37.0 33.0 37.0 27.0 38.5 76-77 31.18710199705062 33.0 30.0 35.0 24.5 37.0 78-79 33.589389752208135 37.0 33.0 37.0 27.0 37.0 80-81 33.94027135097764 37.0 33.0 37.0 27.0 37.0 82-83 33.858150177885506 37.0 33.0 37.0 27.0 37.0 84-85 33.677111024652916 37.0 33.0 37.0 27.0 37.0 86-87 33.50447146337329 37.0 33.0 37.0 27.0 37.0 88-89 33.351149886571896 37.0 33.0 37.0 27.0 37.0 90-91 33.18800854539731 37.0 33.0 37.0 27.0 37.0 92-93 33.065493568594704 37.0 33.0 37.0 27.0 37.0 94-95 32.89243389773303 33.0 33.0 37.0 27.0 37.0 96-97 32.740414318962706 33.0 33.0 37.0 27.0 37.0 98-99 32.61603254334014 33.0 33.0 37.0 27.0 37.0 100-101 31.00213575511355 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1373118677877656 2306 2 0.10120702090884492 2306 3 0.09141211335101929 2306 4 0.12111200983228798 2306 5 0.0884550984533945 2306 6 0.10835893209195291 2306 7 0.12896842120652252 2306 8 0.16046796038336453 2306 9 0.13764759639710178 2306 10-11 0.17599574867652024 2306 12-13 0.20034984806519418 2306 14-15 0.38307841015641486 2306 16-17 0.3125480746917688 2306 18-19 0.33038589295311027 2306 20-21 0.2839791039619186 2306 22-23 0.3041713195154472 2306 24-25 0.3383108194517348 2306 26-27 0.36266988658443466 2306 28-29 0.36473873809843127 2306 30-31 0.41333541089075965 2306 32-33 0.4218483974558396 2306 34-35 0.42131539666652174 2306 36-37 0.3414118452624564 2306 38-39 0.34357058558222064 2306 40-41 0.4082291169610741 2306 42-43 0.44041569247664114 2306 44-45 0.466827877562757 2306 46-47 0.36370346956690724 2306 48-49 0.33267411055992824 2306 50-51 0.29443436701746606 2306 52-53 0.3582104405112645 2306 54-55 0.37158311980009984 2306 56-57 0.36471671030931674 2306 58-59 0.32591571876512404 2306 60-61 0.40950895097730466 2306 62-63 0.32040406711324465 2306 64-65 0.3030512658958884 2306 66-67 0.31856060150674637 2306 68-69 0.28487145560895755 2306 70-71 0.2911714920891342 2306 72-73 0.3142068937150313 2306 74-75 0.3286003004107201 2306 76-77 -0.13200468712932434 2306 78-79 0.09018420625630341 2306 80-81 0.33307245870425106 2306 82-83 0.46059529882550976 2306 84-85 0.49819872413965527 2306 86-87 0.3781714952735271 2306 88-89 0.39819721323218715 2306 90-91 0.3441818259183478 2306 92-93 0.3224987167388491 2306 94-95 0.19062385940681992 2306 96-97 0.3964070988562156 2306 98-99 0.48320149334578133 2306 100-101 0.27619574211021103 2307 1 0.13460033677562677 2307 2 0.11042207093978362 2307 3 0.09718345510584925 2307 4 0.1493252840038437 2307 5 0.12592135460979392 2307 6 0.18515292737310318 2307 7 0.2202896505237959 2307 8 0.21692509576623564 2307 9 0.18000566599268097 2307 10-11 0.17337001732933288 2307 12-13 0.14895549660602825 2307 14-15 0.31470078095384224 2307 16-17 0.3360166097838615 2307 18-19 0.2993335579244132 2307 20-21 0.34238802451107375 2307 22-23 0.33761100318105974 2307 24-25 0.31485154096378665 2307 26-27 0.3171742809560172 2307 28-29 0.3893762967697043 2307 30-31 0.34493464017785413 2307 32-33 0.3368823299530632 2307 34-35 0.2486214733252936 2307 36-37 0.2774134198913103 2307 38-39 0.30117058457606305 2307 40-41 0.2723815088373982 2307 42-43 0.22010614962208308 2307 44-45 0.3284387027588309 2307 46-47 0.2510995465778265 2307 48-49 0.25838171254607545 2307 50-51 0.2252936465115809 2307 52-53 0.17440084483369844 2307 54-55 0.18275722445002884 2307 56-57 0.1802408906722306 2307 58-59 0.17838192461389468 2307 60-61 0.16036158986410243 2307 62-63 0.1510429488752152 2307 64-65 0.10140537628089419 2307 66-67 0.15847153184576968 2307 68-69 0.14017943892694262 2307 70-71 0.1342308249093307 2307 72-73 0.1298798840509292 2307 74-75 0.11742113984385583 2307 76-77 -0.21666636830974184 2307 78-79 0.017666108085997223 2307 80-81 0.2181384874701564 2307 82-83 0.25267255122392385 2307 84-85 0.21854315533641966 2307 86-87 0.1528950799940887 2307 88-89 0.19195119382350612 2307 90-91 0.2784379521301332 2307 92-93 0.20027310713612678 2307 94-95 0.2413113383405019 2307 96-97 0.357713839575716 2307 98-99 0.27115663906183585 2307 100-101 0.09302522942514457 2201 1 0.12121595162629006 2201 2 0.08913314525678828 2201 3 0.08933941256620415 2201 4 0.10860907266418707 2201 5 0.08430828704308624 2201 6 0.10631395817400602 2201 7 0.1319758463674816 2201 8 0.09165763095059276 2201 9 0.11404427857201682 2201 10-11 0.09341926481919671 2201 12-13 0.08566318515811133 2201 14-15 0.22595871468200812 2201 16-17 0.18643770766325218 2201 18-19 0.17463233310537163 2201 20-21 0.10164793238430292 2201 22-23 0.15215254513694987 2201 24-25 0.1117791450309511 2201 26-27 0.1915300303881864 2201 28-29 0.20091337036497947 2201 30-31 0.18771919302337636 2201 32-33 0.2471990953269838 2201 34-35 0.14111202714727966 2201 36-37 0.10865161216879216 2201 38-39 0.204957281385596 2201 40-41 0.254221762292147 2201 42-43 0.17237960055502555 2201 44-45 0.1997661030432809 2201 46-47 0.22732065082542618 2201 48-49 0.24814640719041137 2201 50-51 0.19256715051293583 2201 52-53 0.13081124412045853 2201 54-55 0.17959089742122814 2201 56-57 0.2082079457997068 2201 58-59 0.2200061365479158 2201 60-61 0.12908200422229044 2201 62-63 0.14282981550367424 2201 64-65 0.08487642215323632 2201 66-67 0.07987163585986679 2201 68-69 0.005050270402882973 2201 70-71 0.1256061459732436 2201 72-73 0.10335628983326473 2201 74-75 0.08837308108650888 2201 76-77 -0.0013954009786587562 2201 78-79 0.11527537595412696 2201 80-81 0.17174979316546057 2201 82-83 0.2140549303871353 2201 84-85 0.30747653324722535 2201 86-87 0.08803380083299572 2201 88-89 0.26980043706789303 2201 90-91 0.11545502954633236 2201 92-93 0.12574992685210162 2201 94-95 0.14072360563550035 2201 96-97 0.3512893771587713 2201 98-99 0.1412467492318612 2201 100-101 -0.10605589095525403 1101 1 0.11854229838890262 1101 2 0.12336966600965127 1101 3 0.09092835770093899 1101 4 0.11230014816842271 1101 5 0.10038158328205071 1101 6 0.11556000818168144 1101 7 0.1319810758365847 1101 8 0.06315899990939045 1101 9 0.12604358595788767 1101 10-11 0.09444110308217546 1101 12-13 0.08127060542613407 1101 14-15 0.1895065259748634 1101 16-17 0.13723032086382858 1101 18-19 0.059646301598412776 1101 20-21 0.1570585735404606 1101 22-23 0.1983614705623964 1101 24-25 0.16432246827721997 1101 26-27 0.15380080532577978 1101 28-29 0.2507811229018557 1101 30-31 0.31663275338539876 1101 32-33 0.2689265516667234 1101 34-35 0.2011695164443026 1101 36-37 0.08778591423222792 1101 38-39 0.10087417001417265 1101 40-41 0.105867360008844 1101 42-43 0.14394662840369676 1101 44-45 -0.009766928607795933 1101 46-47 0.13388212381579478 1101 48-49 0.09547624026575363 1101 50-51 0.04174234702475843 1101 52-53 0.10961161552896925 1101 54-55 0.05054323022928742 1101 56-57 0.07410925061035556 1101 58-59 -0.019711565088790906 1101 60-61 0.07288508333369492 1101 62-63 -0.07484921827636981 1101 64-65 -0.0030722662959163927 1101 66-67 0.06274065044195254 1101 68-69 -0.12201060526590624 1101 70-71 -0.10120627180787523 1101 72-73 -0.005701383076221589 1101 74-75 0.07861965636529789 1101 76-77 0.007499461126048601 1101 78-79 0.022327605567340925 1101 80-81 0.18123128549324008 1101 82-83 0.25390214854199655 1101 84-85 0.27232491350632415 1101 86-87 0.16129936024673697 1101 88-89 0.13109713622699815 1101 90-91 0.026705767482532394 1101 92-93 0.014395413006802471 1101 94-95 -0.003568428837162685 1101 96-97 0.08293951870306415 1101 98-99 -0.112244095710885 1101 100-101 -0.14032675188856203 2304 1 0.04393627448404658 2304 2 0.07430637491912506 2304 3 0.07167788840318678 2304 4 0.07310219595819945 2304 5 0.10543418456242648 2304 6 0.08576234109995795 2304 7 0.036860418563364306 2304 8 0.06709286736209918 2304 9 0.012615797193802791 2304 10-11 0.029309557097526806 2304 12-13 0.04497873228941529 2304 14-15 0.11845077663832626 2304 16-17 0.10993787184808212 2304 18-19 0.13591051206098115 2304 20-21 0.11333005001687724 2304 22-23 0.1541143706964263 2304 24-25 0.09448340855934134 2304 26-27 0.12778006337174475 2304 28-29 0.09562119465202557 2304 30-31 0.05280217233315199 2304 32-33 0.10744411920708075 2304 34-35 0.1194837166972107 2304 36-37 0.07394139840599934 2304 38-39 0.15030722230837057 2304 40-41 0.07437446152019334 2304 42-43 0.10787650889591305 2304 44-45 0.03614165680061632 2304 46-47 0.062392437713505444 2304 48-49 0.09783790094991929 2304 50-51 0.019487636675968645 2304 52-53 0.022997768356781023 2304 54-55 -0.003032006656717101 2304 56-57 0.023725025420553436 2304 58-59 0.019928588531179514 2304 60-61 0.007536629103917392 2304 62-63 0.004509697583785055 2304 64-65 0.05110979477365163 2304 66-67 0.01863439316828419 2304 68-69 0.01665879804603776 2304 70-71 0.07496736434200812 2304 72-73 -0.08138976606580428 2304 74-75 -0.13706024960814744 2304 76-77 -0.48958384119339016 2304 78-79 -0.1117967246442646 2304 80-81 0.036898556355211554 2304 82-83 0.1334954778849351 2304 84-85 0.1170300017984971 2304 86-87 0.038406148588208566 2304 88-89 0.0074996944246947805 2304 90-91 -0.10877649013092139 2304 92-93 -0.0695945901073074 2304 94-95 -0.030464484220516397 2304 96-97 -0.08467369884414921 2304 98-99 -0.18579867133052375 2304 100-101 -0.07479183629342856 2202 1 0.04036971361693986 2202 2 0.04124799551922109 2202 3 0.020942869340281334 2202 4 0.007047782900720279 2202 5 0.041216147927158886 2202 6 0.03952474447907406 2202 7 0.038303199947478106 2202 8 0.009295601300983947 2202 9 0.04330756104494782 2202 10-11 0.02525495159711255 2202 12-13 0.061676940215711795 2202 14-15 -0.007175293540157668 2202 16-17 -0.13299114193836914 2202 18-19 -0.13566567960160114 2202 20-21 -0.11696689350412726 2202 22-23 -0.08867965074330186 2202 24-25 -0.13709078179290657 2202 26-27 -0.025887626133481945 2202 28-29 -0.09580155681093316 2202 30-31 -0.03603360321543647 2202 32-33 -0.006914219373790331 2202 34-35 -0.10415190259062257 2202 36-37 -0.14913235840908357 2202 38-39 -0.037418600817595404 2202 40-41 -0.04982195111300314 2202 42-43 -0.1442625567059892 2202 44-45 -0.15993603320193728 2202 46-47 -0.17054269772811637 2202 48-49 -0.3070940016427315 2202 50-51 -0.053045598169511265 2202 52-53 -0.07366158700025949 2202 54-55 -0.13515855145567457 2202 56-57 -0.1793250267985087 2202 58-59 -0.12858734855415577 2202 60-61 -0.22165788192407376 2202 62-63 -0.2987165384175583 2202 64-65 -0.26056936466640224 2202 66-67 -0.15952520256221447 2202 68-69 -0.053109601335741274 2202 70-71 -0.06230597765165413 2202 72-73 -0.268913145719182 2202 74-75 -0.1578646574401219 2202 76-77 -0.46292118595669507 2202 78-79 -0.20449003788921516 2202 80-81 0.01634133918084757 2202 82-83 0.1847074899273906 2202 84-85 0.1008734150008479 2202 86-87 -0.007397970939756249 2202 88-89 0.08884695348242388 2202 90-91 0.12283418869768781 2202 92-93 -0.03551255127900532 2202 94-95 -0.07514406794796713 2202 96-97 0.016607620281241964 2202 98-99 0.006744141214447552 2202 100-101 -0.014374667597380153 1102 1 0.0635336162626885 1102 2 0.07345210468867691 1102 3 0.06397938998765085 1102 4 0.003479072368449465 1102 5 0.010456855503306883 1102 6 -0.03329955115893313 1102 7 0.010857661241296057 1102 8 -0.016080122174209066 1102 9 -0.02951520680142039 1102 10-11 0.05373805838353718 1102 12-13 0.01360178909341414 1102 14-15 -0.0073827834490884925 1102 16-17 -0.059480038106805466 1102 18-19 -0.06003706973289269 1102 20-21 -0.08747221743652034 1102 22-23 -0.07287762282643939 1102 24-25 2.980410731510119E-4 1102 26-27 -0.043589818556625914 1102 28-29 -0.06969207189060711 1102 30-31 -0.07134982394782696 1102 32-33 -0.08711383694119945 1102 34-35 -0.11479020550702757 1102 36-37 -0.060641837091807815 1102 38-39 -0.07495334622376504 1102 40-41 -0.046324138015023664 1102 42-43 -0.17182564317382543 1102 44-45 -0.1155847293160619 1102 46-47 -0.05317686180429604 1102 48-49 -0.1443107661951828 1102 50-51 -0.15984340959219168 1102 52-53 -0.21027130198493893 1102 54-55 -0.1951584850757655 1102 56-57 -0.23440220503689346 1102 58-59 -0.18272637053359375 1102 60-61 -0.22503726482901243 1102 62-63 -0.21181744916049183 1102 64-65 -0.09552698636053236 1102 66-67 -0.1569908991619684 1102 68-69 -0.1871877392380057 1102 70-71 -0.21146283949598654 1102 72-73 -0.18721825477031473 1102 74-75 -0.3010197215478385 1102 76-77 -0.43725265331205776 1102 78-79 -0.117353245326548 1102 80-81 -0.12943021298064394 1102 82-83 -0.15933800275537635 1102 84-85 -0.16965616657495275 1102 86-87 -0.12512232424398917 1102 88-89 -0.11248603418365377 1102 90-91 -0.17996411709729188 1102 92-93 -0.1499020515362517 1102 94-95 -0.2645226787386221 1102 96-97 -0.17569380078624874 1102 98-99 -0.17014844755262004 1102 100-101 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-0.5765537052370675 1213 62-63 -0.21098592808271377 1213 64-65 -0.20711948789587353 1213 66-67 -0.1592372546066727 1213 68-69 -0.17415605804493595 1213 70-71 -0.18475456994720219 1213 72-73 -0.08506752996601818 1213 74-75 0.04092988423017374 1213 76-77 0.17164587529775943 1213 78-79 -0.2664661148617711 1213 80-81 -0.3121993681377262 1213 82-83 -0.28083134655874176 1213 84-85 -0.3598835334499597 1213 86-87 -0.39351206671300076 1213 88-89 -0.5589557943856462 1213 90-91 -0.38531450242128784 1213 92-93 -0.34459135129257845 1213 94-95 -0.24454217889711316 1213 96-97 -0.32930702081976193 1213 98-99 -0.3441473619861526 1213 100-101 -0.04753094334461139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 16.0 10 16.0 11 18.0 12 50.0 13 168.0 14 492.0 15 1431.0 16 2992.0 17 4947.0 18 6300.0 19 7753.0 20 10091.0 21 13114.0 22 16995.0 23 21778.0 24 27601.0 25 35376.0 26 45584.0 27 59431.0 28 82424.0 29 111289.0 30 123622.0 31 142331.0 32 191371.0 33 273560.0 34 419328.0 35 715493.0 36 1357254.0 37 2237665.0 38 822646.0 39 437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.02651412751235 27.64362029843569 20.17795209728195 16.151913476770012 2 30.623403698717254 24.639649817568753 30.165487225355957 14.571459258358036 3 35.72187295449596 20.95546933054989 25.448944961883612 17.87371275307054 4 29.657230076968055 21.32867060143477 30.06169567429058 18.952403647306593 5 30.552424788426247 19.703332517774065 31.480409419664962 18.263833274134726 6 27.54247630235827 20.11374154971413 33.74314815087163 18.600633997055965 7 31.964352483823628 21.244813808116582 26.317488457756628 20.47334525030316 8 29.196442378955183 27.711262903180046 23.512142845576896 19.58015187228788 9 27.982076116785116 24.841935255290103 26.673437739774798 20.502550888149983 10-11 22.738861933838088 29.3078575792864 30.85671455589084 17.09656593098467 12-13 22.134889997495385 28.220450373122247 30.76043582080506 18.88422380857731 14-15 21.621159499475382 28.37157622946302 31.344208051100065 18.663056219961533 16-17 21.883581060097885 27.75664066210939 31.95762537943378 18.40215289835895 18-19 20.907092052872603 27.81583721021092 32.50051956621755 18.776551170698934 20-21 21.647579757313924 28.17732754152556 31.893291869052376 18.28180083210814 22-23 21.07050690121529 29.718135507210206 30.70922703298845 18.502130558586053 24-25 21.0533747382113 28.14746595669898 31.39457428530088 19.404585019788833 26-27 21.099595236004877 28.307193099031625 30.669083930853052 19.924127734110446 28-29 21.673316668590438 29.096800051458967 30.41421794625539 18.81566533369521 30-31 20.861545518977202 28.705680704536245 30.972214980234202 19.46055879625235 32-33 20.740192082776442 29.392867377139115 30.35505647487951 19.511884065204928 34-35 21.52373062062575 29.22188218303081 30.53123361732325 18.72315357902019 36-37 21.4462450626354 29.06832975393433 30.71027784425551 18.775147339174758 38-39 21.09487901692337 28.513106995151173 31.50304091795018 18.888973069975282 40-41 20.989026791196178 28.675308920925957 29.9197052934253 20.415958994452563 42-43 20.771618596949867 29.180391162427465 30.754041734945613 19.29394850567705 44-45 20.697300823240223 28.427643413402308 30.948852801225748 19.926202962131722 46-47 21.394921620947905 28.49727951407097 30.105698298660123 20.002100566320998 48-49 20.854495312424792 29.487706294013798 30.26646563900709 19.39133275455432 50-51 21.56986496504856 28.801011534707406 30.0501288447013 19.578994655542736 52-53 20.777966063674768 28.759008593789577 29.48092168018101 20.982103662354646 54-55 21.391224204566523 28.91600647946111 30.00375552037726 19.689013795595105 56-57 20.70054790169591 28.83575383539358 30.207966732215997 20.25573153069451 58-59 20.721471892451085 28.905122848729402 30.319620104838265 20.053785153981245 60-61 21.169108582949736 28.95944447134961 30.278719541163568 19.592727404537083 62-63 20.306572547295207 28.880190179859017 30.2847164218793 20.528520850966476 64-65 21.064861203978847 28.721560746443174 30.195341392858083 20.018236656719896 66-67 20.52468156828779 29.79404356090854 29.611805613846904 20.06946925695677 68-69 20.46566330710503 30.053558218275754 29.51909769693199 19.961680777687228 70-71 20.27528872326639 30.328134750870582 29.363972010272814 20.032604515590215 72-73 20.277334917207256 30.20011140698976 29.368934148277663 20.15361952752532 74-75 20.39145722954376 30.052014729508848 29.37628083170083 20.18024720924656 76-77 20.4556275826312 29.867127501294334 29.424335544656167 20.252909371418298 78-79 20.309638011614283 29.80455647399577 29.65641970445589 20.229385809934055 80-81 20.20851345770457 30.060752050818103 29.51913106517754 20.211603426299792 82-83 20.10239948232041 29.89850669464228 29.496483513447146 20.50261030959016 84-85 20.20910646542318 29.667548930431593 29.635186384348206 20.488158219797022 86-87 20.312543290048918 29.62035040270098 29.77355585882369 20.293550448426412 88-89 20.518284106734992 29.865636786641808 29.37307739647031 20.243001710152893 90-91 20.586387867021674 29.757409663917358 29.29413017880175 20.362072290259217 92-93 20.308482316593906 29.87540234635775 29.269859517599617 20.546255819448728 94-95 20.069255327691458 29.917942034021667 29.214164628037487 20.79863801024939 96-97 20.112435721514668 30.246633866538463 29.056311905091587 20.584618506855282 98-99 20.37507119012054 30.189469158475017 28.934376283919903 20.501083367484537 100-101 20.464824920349123 29.99891473799911 28.661390604661786 20.874869736989986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 134.0 1 83.0 2 32.0 3 37.5 4 40.5 5 38.5 6 44.5 7 57.0 8 59.5 9 64.5 10 88.5 11 109.5 12 121.5 13 136.5 14 166.5 15 207.0 16 235.0 17 269.0 18 334.0 19 445.0 20 660.5 21 1113.5 22 1936.5 23 3395.0 24 5750.0 25 9598.0 26 16303.5 27 25822.5 28 37146.5 29 50891.5 30 67089.0 31 85359.5 32 110094.0 33 144615.0 34 186322.5 35 232143.5 36 286127.5 37 346387.5 38 400457.0 39 433024.5 40 451046.5 41 446627.0 42 420246.5 43 398432.0 44 369617.0 45 338786.0 46 319645.5 47 292564.5 48 251656.0 49 212840.0 50 175236.5 51 140056.0 52 112289.5 53 89599.0 54 72770.5 55 57077.5 56 40651.0 57 27641.5 58 19389.0 59 14463.5 60 10549.5 61 7328.0 62 5026.0 63 3336.5 64 2253.5 65 1549.5 66 1098.5 67 771.0 68 571.0 69 420.5 70 298.5 71 217.0 72 152.0 73 111.5 74 92.0 75 79.0 76 63.0 77 44.5 78 30.0 79 21.5 80 11.5 81 10.5 82 6.5 83 4.0 84 6.0 85 5.0 86 4.0 87 4.0 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15345586925619362 2 0.0 3 0.005214231375500867 4 0.007026585300888633 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.456608013248604E-5 14-15 0.0 16-17 7.427680022081007E-6 18-19 0.0 20-21 0.0 22-23 1.3369824039745814E-4 24-25 4.456608013248604E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.467555225172349E-4 40-41 0.0 42-43 0.0 44-45 8.913216026497208E-5 46-47 8.764662426055589E-4 48-49 0.0024511344072867323 50-51 3.491009610378073E-4 52-53 0.0013072716838862571 54-55 0.002272870086756788 56-57 0.003862393611482124 58-59 0.005221659055522948 60-61 0.0017083664050786315 62-63 8.096171224068298E-4 64-65 0.0011884288035329611 66-67 0.008051605143935811 68-69 0.007286554101661467 70-71 0.009024631226828422 72-73 0.005964427057731049 74-75 0.008898360666453047 76-77 0.004634872333778548 78-79 0.0044194696131382 80-81 0.0017380771251669558 82-83 0.0 84-85 4.4566080132486043E-4 86-87 0.0013889761641291483 88-89 0.00456802321357982 90-91 0.006462081619210476 92-93 0.008786945466121831 94-95 0.008059032823957892 96-97 0.010532450271310868 98-99 0.007152855861264009 100-101 0.007182566581352335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6731577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.07977163343833 #Duplication Level Percentage of deduplicated Percentage of total 1 91.62224022410007 60.54376710552559 2 4.784828777578404 6.323607858549696 3 1.2974520739131108 2.5720601024852794 4 0.5119868633026565 1.3532790002543977 5 0.3110408739275417 1.027675495889852 6 0.2196936420640469 0.8710383418146336 7 0.17010583243476862 0.7868388192563806 8 0.07790411678548308 0.4118308997191545 9 0.10189819902897024 0.60600687495237 >10 0.7519529419512714 9.93872696892591 >50 0.08908916815196521 4.067874090670701 >100 0.05599300379054632 7.556004361111904 >500 0.004983330133155081 2.1347563714806452 >1k 8.084380575786662E-4 0.6972810082952796 >5k 0.0 0.0 >10k+ 2.2514780215294052E-5 1.1092527010680484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 74558 1.1075859341726315 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.319001624730728E-4 0.0 0.0 2 9.358876827822069E-4 0.0 0.0 3 0.001054730563135503 0.0 0.0 4 0.001099296643267989 0.0 0.0 5 0.0012627056037537712 0.0 0.0 6 0.0015895235247253355 0.0 0.0 7 0.0019163414456968998 0.0 0.0 8 0.0024065683271542464 0.0 0.0 9 0.0026591094479050006 0.0 0.0 10-11 0.004753715214131845 0.0 0.0 12-13 0.007145428181241928 0.0 0.0 14-15 0.010250198430471789 0.0 2.971072008832403E-5 16-17 0.015241599405310228 0.0 5.199376015456705E-5 18-19 0.02041869238070069 0.0 7.427680022081007E-5 20-21 0.02277326694770037 0.0 8.170448024289107E-5 22-23 0.025202118314920856 0.0 8.913216026497208E-5 24-25 0.027757240242516724 0.0 9.65598402870531E-5 26-27 0.030483198810620456 0.0 1.039875203091341E-4 28-29 0.03374395034031402 0.0 1.1884288035329612E-4 30-31 0.05274395583679723 0.0 1.3369824039745812E-4 32-33 0.05797304257234226 0.0 1.4112592041953912E-4 34-35 0.06203598354442057 0.0 1.4855360044162014E-4 36-37 0.06656686835788998 0.0 1.4855360044162014E-4 38-39 0.07165482917301547 0.0 1.4855360044162014E-4 40-41 0.07708446326915669 0.0 1.7826432052994417E-4 42-43 0.08338313592788138 0.0 1.7826432052994417E-4 44-45 0.09024631226828424 0.0 1.7826432052994417E-4 46-47 0.09728032524919494 0.0 1.7826432052994417E-4 48-49 0.10512395535251248 0.0 1.7826432052994417E-4 50-51 0.11316813281642622 0.0 1.7826432052994417E-4 52-53 0.12188822916234933 0.0 1.7826432052994417E-4 54-55 0.13184132039193786 0.0 1.7826432052994417E-4 56-57 0.14268573322417616 0.0 1.931196805741062E-4 58-59 0.1547482855800357 0.0 2.079750406182682E-4 60-61 0.16743476305775007 0.0 2.079750406182682E-4 62-63 0.18246838742244204 0.0 2.302580806845112E-4 64-65 0.19905439691174892 0.0 2.3768576070659224E-4 66-67 0.2175121817666202 0.0 2.3768576070659224E-4 68-69 0.23683157750405293 0.0 2.5254112075075424E-4 70-71 0.25969397661201826 0.0 2.5254112075075424E-4 72-73 0.28667873813223854 0.0 2.5996880077283526E-4 74-75 0.3164563073407613 0.0 2.971072008832403E-4 76-77 0.3504156604017157 0.0 3.045348809053213E-4 78-79 0.3877248971526286 0.0 3.2681792097156434E-4 80-81 0.4297729937576291 0.0 3.2681792097156434E-4 82-83 0.476522811816607 0.0 3.4167328101572633E-4 84-85 0.5315099270200727 0.0 3.5652864105988833E-4 86-87 0.5923871924810487 0.0 3.5652864105988833E-4 88-89 0.6617542962072631 0.0 3.6395632108196936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 15240 0.0 53.73781 4 TCGGAAG 15295 0.0 53.57762 3 GATCGGA 15880 0.0 51.980267 1 ATCGGAA 15990 0.0 51.248882 2 GAAGAGC 17595 0.0 46.727673 6 GCCGTCT 9680 0.0 42.05213 50-51 TATGCCG 9625 0.0 41.79878 48-49 ATGCCGT 9855 0.0 41.232937 48-49 TGCCGTC 9800 0.0 41.125225 50-51 TCTCGTA 9770 0.0 41.056732 42-43 CGTATGC 9810 0.0 41.03458 46-47 TCGTATG 10075 0.0 40.568142 44-45 CTCGTAT 9845 0.0 40.52685 44-45 CCGTCTT 10025 0.0 40.344654 52-53 CATCTCG 10020 0.0 39.888966 40-41 ATCTCGT 10155 0.0 38.985664 42-43 CGTCTTC 10525 0.0 38.743954 52-53 GTATGCC 10525 0.0 38.653122 46-47 CGAGATT 10530 0.0 38.31844 32-33 AGAGCAC 21480 0.0 38.232006 8 >>END_MODULE