FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5697607
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2041203.582556676864515TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG273050.077.780733
GATCGGA277850.076.621081
CGGAAGA276750.076.5834
ATCGGAA277750.076.498752
GAAGAGC301600.070.285086
AGAGCAC314000.067.388488
GAGCACA314750.067.303359
AAGAGCA343350.061.655697
GGAAGAG370250.057.5482035
GCCGTCT233400.045.03834550-51
ATGCCGT233800.044.74757448-49
TGCCGTC233950.044.72942750-51
CGTATGC234250.044.69163546-47
TATGCCG233450.044.66205648-49
TCTCGTA234250.044.6612242-43
CTCGTAT234850.044.39542444-45
TCGTATG238700.044.3161544-45
CGTCTTC238000.044.25747752-53
ATCTCGT235800.044.24677742-43
CCGTCTT236950.044.19298652-53
Produced by FastQC (version 0.11.2)