##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5697607 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.680683662456886 33.0 33.0 33.0 33.0 33.0 2 32.71498701121366 33.0 33.0 33.0 33.0 33.0 3 32.75029692290114 33.0 33.0 33.0 33.0 33.0 4 36.68836039410932 37.0 37.0 37.0 37.0 37.0 5 36.68565732947183 37.0 37.0 37.0 37.0 37.0 6 36.570024924499 37.0 37.0 37.0 37.0 37.0 7 36.49260206960572 37.0 37.0 37.0 37.0 37.0 8 36.437432592314636 37.0 37.0 37.0 37.0 37.0 9 36.46774742448891 37.0 37.0 37.0 37.0 37.0 10-11 36.399165912987684 37.0 37.0 37.0 37.0 37.0 12-13 36.38679580743284 37.0 37.0 37.0 37.0 37.0 14-15 38.26165958796386 40.0 37.0 40.0 37.0 40.0 16-17 38.243667385272445 40.0 37.0 40.0 37.0 40.0 18-19 38.21682752074687 40.0 37.0 40.0 37.0 40.0 20-21 38.18934545678563 40.0 37.0 40.0 37.0 40.0 22-23 38.05477115216967 40.0 37.0 40.0 35.0 40.0 24-25 38.04395740176534 40.0 37.0 40.0 35.0 40.0 26-27 37.97325499986222 40.0 37.0 40.0 33.0 40.0 28-29 37.805497904646636 40.0 37.0 40.0 33.0 40.0 30-31 37.777702287995645 40.0 37.0 40.0 33.0 40.0 32-33 37.63108503271637 40.0 37.0 40.0 33.0 40.0 34-35 37.50227595550202 40.0 37.0 40.0 33.0 40.0 36-37 37.29906731018829 40.0 37.0 40.0 33.0 40.0 38-39 37.32555149907672 40.0 37.0 40.0 33.0 40.0 40-41 37.19427252529 40.0 37.0 40.0 33.0 40.0 42-43 37.04914387742082 40.0 37.0 40.0 33.0 40.0 44-45 36.9920515753368 40.0 37.0 40.0 33.0 40.0 46-47 36.96128374596563 40.0 37.0 40.0 33.0 40.0 48-49 36.72431820938159 37.0 37.0 40.0 33.0 40.0 50-51 36.63680717185302 37.0 37.0 40.0 33.0 40.0 52-53 36.52305792589766 37.0 37.0 40.0 33.0 40.0 54-55 36.291349947442846 37.0 37.0 40.0 33.0 40.0 56-57 36.1474490957344 37.0 37.0 40.0 33.0 40.0 58-59 35.930212455860854 37.0 37.0 40.0 27.0 40.0 60-61 35.69812405453728 37.0 37.0 40.0 27.0 40.0 62-63 35.48287087894971 37.0 33.0 40.0 27.0 40.0 64-65 35.24337305117745 37.0 33.0 40.0 27.0 40.0 66-67 34.852290444040804 37.0 33.0 40.0 27.0 40.0 68-69 34.63282804517756 37.0 33.0 37.0 27.0 40.0 70-71 34.221680698581 37.0 33.0 37.0 27.0 40.0 72-73 33.69293854419935 37.0 33.0 37.0 24.5 40.0 74-75 33.163222910951916 37.0 33.0 37.0 22.0 40.0 76-77 30.550917692287307 33.0 30.0 35.0 22.0 37.0 78-79 32.785763654811575 37.0 33.0 37.0 22.0 37.0 80-81 33.07093109440507 37.0 33.0 37.0 22.0 37.0 82-83 32.93675871291228 37.0 33.0 37.0 22.0 37.0 84-85 32.71202322308295 37.0 33.0 37.0 22.0 37.0 86-87 32.50831120854773 37.0 33.0 37.0 22.0 37.0 88-89 32.31772768813293 37.0 33.0 37.0 22.0 37.0 90-91 32.13176329992574 35.0 33.0 37.0 22.0 37.0 92-93 31.99110222940965 33.0 33.0 37.0 22.0 37.0 94-95 31.7909758430162 33.0 33.0 37.0 18.5 37.0 96-97 31.62412131970492 33.0 33.0 37.0 15.0 37.0 98-99 31.47625090674032 33.0 33.0 37.0 15.0 37.0 100-101 29.875509577968437 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11516886321676623 2306 2 0.13112461058368297 2306 3 0.10541542564896389 2306 4 0.15522254372152844 2306 5 0.15730079245200557 2306 6 0.1521250911111025 2306 7 0.22432544165654633 2306 8 0.2346903003655072 2306 9 0.21487465367805925 2306 10-11 0.1582628578490457 2306 12-13 0.22461870538369055 2306 14-15 0.4037641208867697 2306 16-17 0.32339727875659463 2306 18-19 0.4004381076890553 2306 20-21 0.39842061589900624 2306 22-23 0.43116703046266736 2306 24-25 0.3976607284049223 2306 26-27 0.41314213026858937 2306 28-29 0.49974771011305563 2306 30-31 0.4847689828184585 2306 32-33 0.4634781424116312 2306 34-35 0.5042851980206464 2306 36-37 0.38688254255942667 2306 38-39 0.47378728137329773 2306 40-41 0.5629770654642883 2306 42-43 0.560725040122577 2306 44-45 0.6018160331879656 2306 46-47 0.44392514442108677 2306 48-49 0.5579840352308381 2306 50-51 0.4015100305197805 2306 52-53 0.39047749642595164 2306 54-55 0.3935496719816669 2306 56-57 0.4643794768385803 2306 58-59 0.4201360479131395 2306 60-61 0.492816103883122 2306 62-63 0.4949061447818579 2306 64-65 0.4566036665027582 2306 66-67 0.46264171335832316 2306 68-69 0.34140194806787605 2306 70-71 0.46058740385075936 2306 72-73 0.4272213520895889 2306 74-75 0.24312462223587517 2306 76-77 -0.09960553489819901 2306 78-79 0.3033996289998271 2306 80-81 0.42895753416007665 2306 82-83 0.4255327202815522 2306 84-85 0.5821918450748171 2306 86-87 0.5514666206543453 2306 88-89 0.4177401844007207 2306 90-91 0.4618796402864902 2306 92-93 0.5415902179404881 2306 94-95 0.41811252264811927 2306 96-97 0.4900586857875986 2306 98-99 0.45080619986243775 2306 100-101 0.38496737460894437 2307 1 0.1408700691043947 2307 2 0.1486024158470869 2307 3 0.11359685219458271 2307 4 0.13542032960144468 2307 5 0.11121457367305254 2307 6 0.1059391524777098 2307 7 0.13076886487138495 2307 8 0.12150694602837575 2307 9 0.16115719552772134 2307 10-11 0.1663833654287501 2307 12-13 0.20689672157311634 2307 14-15 0.334855156631086 2307 16-17 0.34356249325418986 2307 18-19 0.23748367545255888 2307 20-21 0.26509215440733414 2307 22-23 0.27802692953385844 2307 24-25 0.2947748471277194 2307 26-27 0.3546409467001084 2307 28-29 0.30958125650044366 2307 30-31 0.33956091454599147 2307 32-33 0.3520414878311513 2307 34-35 0.2821659770595488 2307 36-37 0.22283232296210542 2307 38-39 0.32258823023881433 2307 40-41 0.22436095711989168 2307 42-43 0.0717122264668788 2307 44-45 0.21256661894000217 2307 46-47 0.17349690074220803 2307 48-49 0.13840382502866788 2307 50-51 0.053434202713035006 2307 52-53 0.14246771495758281 2307 54-55 0.10813262934957635 2307 56-57 0.1216484635742674 2307 58-59 0.08641073658863974 2307 60-61 0.08608561692852135 2307 62-63 0.13399859608313136 2307 64-65 0.07039617908115758 2307 66-67 0.04407978299306592 2307 68-69 -0.0032675701551667657 2307 70-71 0.07885898418236081 2307 72-73 -0.12423588361320981 2307 74-75 -0.0860837943963162 2307 76-77 -0.3348474677064992 2307 78-79 -0.09606752012577857 2307 80-81 0.011120410676355164 2307 82-83 0.06658152180850152 2307 84-85 0.1215463767421241 2307 86-87 0.01612774531836436 2307 88-89 0.031090272596550506 2307 90-91 -0.04214349346303692 2307 92-93 0.009293772114446597 2307 94-95 0.025490258691988288 2307 96-97 0.13517727638349797 2307 98-99 -0.06018761562152264 2307 100-101 -0.17761217506700078 2201 1 0.1547357941544476 2201 2 0.13771287189008774 2201 3 0.06605564708444689 2201 4 0.09393304975800021 2201 5 0.08318671025850222 2201 6 0.07356586424348421 2201 7 0.15301376926471733 2201 8 0.11396977191412816 2201 9 0.0878452595012007 2201 10-11 0.1273925725386178 2201 12-13 0.10176395768363022 2201 14-15 0.20706490240811348 2201 16-17 0.20725352304977918 2201 18-19 0.12125745326136439 2201 20-21 0.12629747847463335 2201 22-23 0.25253600377850205 2201 24-25 0.14317303295438677 2201 26-27 0.15022385347601386 2201 28-29 0.20307896739021203 2201 30-31 0.21081957612023672 2201 32-33 0.32596319643293725 2201 34-35 0.21022593080443386 2201 36-37 0.09868029586660754 2201 38-39 0.14682498706617508 2201 40-41 0.16470765091508355 2201 42-43 0.2589556727206386 2201 44-45 0.1623120638020552 2201 46-47 0.16510325260085779 2201 48-49 0.19007840543353183 2201 50-51 0.33122510437249986 2201 52-53 0.20502823619462163 2201 54-55 0.15037270893359533 2201 56-57 0.08067685281984183 2201 58-59 0.12553084788014957 2201 60-61 0.04316477195070689 2201 62-63 0.13507928018141513 2201 64-65 0.08565099319369551 2201 66-67 0.22158339174775676 2201 68-69 0.02664692318919748 2201 70-71 0.14313721072432628 2201 72-73 0.06983509006371236 2201 74-75 -0.026240001116597966 2201 76-77 -0.0661829715472031 2201 78-79 0.1907835233166466 2201 80-81 0.2590027866669402 2201 82-83 0.18110746318116355 2201 84-85 0.12148765706749032 2201 86-87 0.15536225582259533 2201 88-89 0.1930139415234322 2201 90-91 0.20661086688097186 2201 92-93 0.11259302401565918 2201 94-95 0.05160165379939485 2201 96-97 0.21473325880925742 2201 98-99 0.14595109567584785 2201 100-101 0.002826995178395464 1101 1 0.0787811247712682 1101 2 0.08584357545205279 1101 3 0.04094843193708897 1101 4 0.07354617733896163 1101 5 0.06062779788917538 1101 6 0.07254802377302383 1101 7 0.11011150330912756 1101 8 0.08022997101528517 1101 9 0.020007702798537252 1101 10-11 0.035815917741253145 1101 12-13 0.08051423686694648 1101 14-15 0.06820608625542235 1101 16-17 0.10331219243896328 1101 18-19 2.5178805974945817E-4 1101 20-21 0.030115955920749116 1101 22-23 0.13042080842770787 1101 24-25 0.11469881073471555 1101 26-27 0.06415923401193879 1101 28-29 0.07531987964499365 1101 30-31 0.21685031092072649 1101 32-33 0.1257688873456786 1101 34-35 0.13169557179156755 1101 36-37 0.09344833173413036 1101 38-39 0.16421576292442097 1101 40-41 0.11853526981716556 1101 42-43 0.10134191536715065 1101 44-45 -0.02498041201685197 1101 46-47 0.028582273146994908 1101 48-49 -0.013091016133650157 1101 50-51 0.19215447155615095 1101 52-53 0.2254652052929984 1101 54-55 0.10085668087245381 1101 56-57 0.017384963254627905 1101 58-59 -0.014104004102044598 1101 60-61 -0.03978569986596625 1101 62-63 -0.07963468728800649 1101 64-65 0.015503486860332316 1101 66-67 -0.04286597371514489 1101 68-69 -0.13386136177113173 1101 70-71 -0.20457356111218417 1101 72-73 -0.15051524154228701 1101 74-75 -0.0986564143194073 1101 76-77 -0.18261053057337406 1101 78-79 -0.043042189760683414 1101 80-81 0.13929513317989262 1101 82-83 0.23160621651666702 1101 84-85 0.2744192632200537 1101 86-87 0.2004415388823375 1101 88-89 0.04739439966669323 1101 90-91 0.07876596700054961 1101 92-93 0.0365091237077948 1101 94-95 0.046305736991396174 1101 96-97 0.050879150944080465 1101 98-99 0.02681816590048669 1101 100-101 -0.01922484775663591 2304 1 0.07372653715217581 2304 2 0.07977186047897789 2304 3 0.06369751125839684 2304 4 0.09996753251662227 2304 5 0.08988778599056957 2304 6 0.11723377424706172 2304 7 0.06561039713851358 2304 8 0.10348110082964723 2304 9 0.086014360878508 2304 10-11 0.039453129470409465 2304 12-13 0.02351446456071926 2304 14-15 0.09847787071436898 2304 16-17 0.13183824285492562 2304 18-19 0.061984035350889144 2304 20-21 0.026239161627792384 2304 22-23 0.050252057185296906 2304 24-25 0.07962734504706503 2304 26-27 0.039679298365761895 2304 28-29 0.043069514817460686 2304 30-31 -0.0052385546039772635 2304 32-33 0.04878778200305334 2304 34-35 0.10036811620523878 2304 36-37 0.1541887460225908 2304 38-39 0.18141108153331942 2304 40-41 0.2598981053755054 2304 42-43 0.13223559736459833 2304 44-45 0.15911328200768793 2304 46-47 0.1828640982589107 2304 48-49 0.1819416818341253 2304 50-51 0.0437394414830834 2304 52-53 0.025495134711498224 2304 54-55 0.04771750912104267 2304 56-57 0.15144832636303107 2304 58-59 0.09306463811145704 2304 60-61 0.10219809640306465 2304 62-63 0.059536194211659677 2304 64-65 -0.03469835046424663 2304 66-67 0.07247238708241355 2304 68-69 -0.00622689039719404 2304 70-71 0.02689444541456254 2304 72-73 0.04710243206260856 2304 74-75 0.06172476218645073 2304 76-77 -0.3598224435258288 2304 78-79 -0.02318951441239392 2304 80-81 0.23995951362977053 2304 82-83 0.2707521248631366 2304 84-85 0.20145505254121332 2304 86-87 0.1033370149496946 2304 88-89 0.1411052871049563 2304 90-91 0.1693375443937697 2304 92-93 0.00721873331290368 2304 94-95 0.1316335544609366 2304 96-97 0.06643247803902952 2304 98-99 0.12647730778761357 2304 100-101 0.14404555037556221 2202 1 0.0522722035732599 2202 2 0.06543847417673732 2202 3 0.06914163146892349 2202 4 0.04525269785184349 2202 5 0.06051821229396381 2202 6 0.07251658005822748 2202 7 0.08448610800532919 2202 8 0.07470080622621822 2202 9 0.09810547702969075 2202 10-11 0.07125710599074608 2202 12-13 0.12203073323827596 2202 14-15 0.09881416308346758 2202 16-17 0.06498642578064562 2202 18-19 0.05781411831945604 2202 20-21 0.0514555516523032 2202 22-23 0.018955197759964904 2202 24-25 -0.07422800890562797 2202 26-27 0.08076198282999059 2202 28-29 0.09406972428541849 2202 30-31 0.02649078391068116 2202 32-33 0.006840982047350508 2202 34-35 -0.09524816499224187 2202 36-37 -0.052471070801296094 2202 38-39 0.005568815716081588 2202 40-41 -0.0010136700941103527 2202 42-43 -0.061822133928949086 2202 44-45 0.003345509735460439 2202 46-47 -0.07610094948533686 2202 48-49 -0.22649855238475425 2202 50-51 0.05534131754546934 2202 52-53 0.07287287284285782 2202 54-55 -0.002895786556216251 2202 56-57 -0.03285000415332462 2202 58-59 -0.06755105320501542 2202 60-61 -0.15401527680140958 2202 62-63 -0.16434658318891593 2202 64-65 -0.17867458187095764 2202 66-67 0.02071105053242661 2202 68-69 -0.045380001838957185 2202 70-71 -0.1483150827287787 2202 72-73 -0.17769327649282474 2202 74-75 -0.0076486156407682415 2202 76-77 -0.43921958513098147 2202 78-79 -0.10111050887955031 2202 80-81 0.05106757501592085 2202 82-83 0.14400264612534386 2202 84-85 0.15455866935450757 2202 86-87 0.10458828499962891 2202 88-89 0.018408351115468236 2202 90-91 0.08150847505746839 2202 92-93 -0.06032727740607413 2202 94-95 -0.015530261515451116 2202 96-97 0.024617317856240106 2202 98-99 -0.0348388371353181 2202 100-101 -0.06661384028938855 1102 1 0.08432339819009371 1102 2 0.07639590103345029 1102 3 0.0947359917067061 1102 4 0.053199038130095744 1102 5 0.05828455728930493 1102 6 0.006580706885387144 1102 7 0.03220045136389871 1102 8 0.03991604469492671 1102 9 0.007613997625753655 1102 10-11 0.0075433615034867785 1102 12-13 0.031396178292723675 1102 14-15 -3.065332837977053E-4 1102 16-17 -0.05846819497681821 1102 18-19 -0.04308882403544345 1102 20-21 -0.03139156282517064 1102 22-23 0.002932388790668483 1102 24-25 -0.011571293590428411 1102 26-27 0.041141140119307806 1102 28-29 -0.09136518202638655 1102 30-31 -0.0018767660734084757 1102 32-33 0.04539064076885069 1102 34-35 0.026070605751584708 1102 36-37 0.010643642536130926 1102 38-39 0.02152005139432589 1102 40-41 0.05979464277956481 1102 42-43 -0.08668191776695267 1102 44-45 -0.09114045195382658 1102 46-47 -0.09313803901339668 1102 48-49 -0.08914105960246133 1102 50-51 -0.10610694579877133 1102 52-53 -0.1044002451525543 1102 54-55 -0.19086998180957693 1102 56-57 -0.24525495198118819 1102 58-59 -0.14320807115267797 1102 60-61 -0.09806616878577046 1102 62-63 -0.1926510492201956 1102 64-65 -0.12693922260501012 1102 66-67 -0.056573931224377816 1102 68-69 -0.03303154895869653 1102 70-71 -0.06608719265967267 1102 72-73 -0.12286754946448042 1102 74-75 -0.2000754332826773 1102 76-77 -0.340245249927861 1102 78-79 -0.10211986988457511 1102 80-81 -0.010921022275439896 1102 82-83 0.004038500259333944 1102 84-85 -0.007423338931658918 1102 86-87 0.011750391577400876 1102 88-89 -0.01849650720138385 1102 90-91 -0.0019391446599570372 1102 92-93 -0.037949044353489114 1102 94-95 -0.22594740770540866 1102 96-97 -0.15012759351364835 1102 98-99 -0.10051658469720692 1102 100-101 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-0.10880944914959656 1213 72-73 0.010662037614856956 1213 74-75 0.015228966225841134 1213 76-77 0.11623718945685368 1213 78-79 -0.39522543073577765 1213 80-81 -0.490261292450171 1213 82-83 -0.47516923026452673 1213 84-85 -0.44506926609160047 1213 86-87 -0.4668503539203286 1213 88-89 -0.612625352738533 1213 90-91 -0.43913012828186027 1213 92-93 -0.4451593139348802 1213 94-95 -0.2106316668907624 1213 96-97 -0.3098058443231224 1213 98-99 -0.36327970388219555 1213 100-101 -0.013574232379326645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 1.0 9 19.0 10 30.0 11 31.0 12 86.0 13 198.0 14 602.0 15 1390.0 16 2833.0 17 4727.0 18 6952.0 19 9823.0 20 12846.0 21 16606.0 22 21667.0 23 27109.0 24 34522.0 25 43502.0 26 55934.0 27 76638.0 28 115398.0 29 158159.0 30 138196.0 31 134581.0 32 174737.0 33 242655.0 34 360962.0 35 579253.0 36 1017658.0 37 1669373.0 38 786541.0 39 4577.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.20154721523758 27.152171895303507 19.19699360413116 16.449287285327756 2 29.44025447876626 27.567240071138638 28.17482848501134 14.817676965083763 3 33.61969039456474 21.71417207681652 27.644719022079872 17.02141850653887 4 29.18027198884228 22.307831241874325 27.98249461711056 20.52940215217284 5 32.98805972402098 20.533883786649376 29.259810302816604 17.21824618651304 6 30.37870109328355 21.30638353961584 30.821518577887176 17.493396789213435 7 30.833032183511428 25.012676374484936 24.98194417410678 19.172347267896857 8 28.471830366678503 30.546701448520402 22.74886632229987 18.232601862501223 9 30.925158579733562 25.283017940689838 24.653543145394195 19.138280334182404 10-11 26.08319598034754 29.90430894935365 27.810789687670628 16.20170538262818 12-13 24.05671752547211 29.54758702078979 27.182671874001773 19.213023579736323 14-15 23.25521925257393 29.83563976946813 27.71723286635951 19.19190811159843 16-17 24.735529273171924 27.876409846521504 27.69762423024688 19.69043665005969 18-19 21.953813241243207 28.301364063895594 29.89546313039843 19.849359564462766 20-21 23.648156146957835 28.06611617824817 30.111316909011098 18.1744107657829 22-23 21.579840634653188 32.50447556866049 27.594364293737716 18.32131950294861 24-25 21.193106577237277 28.35115807431368 30.131046290502766 20.324689057946276 26-27 21.350630185620034 28.16257772780748 27.84853360366905 22.638258482903435 28-29 23.052432363271105 30.67773891740866 27.760233375169612 18.509595344150622 30-31 20.854676357986783 28.408093081885077 30.07027336213256 20.66695719799558 32-33 20.939440014026943 30.663311807922167 27.657663998236455 20.73958417981444 34-35 22.665901667138503 30.593159549263397 28.16191078114022 18.57902800245787 36-37 20.838494125691717 30.232806509820705 30.00576382330336 18.92293554118422 38-39 20.67463840979819 28.660390508817752 29.905217005020234 20.75975407636382 40-41 22.555794037742512 28.326462320058226 30.124778700952877 18.992964941246388 42-43 20.617787432513335 30.361737831338665 29.82137939664845 19.199095339499546 44-45 20.28646094088204 28.294823408555935 30.330452266704206 21.088263383857818 46-47 22.547565248312342 28.13112215759006 27.92203199204148 21.399280602056116 48-49 20.327744407454066 30.561011965487932 29.876720677884887 19.234522949173112 50-51 22.34095095077175 28.154320552121938 30.07811910385844 19.426609393247873 52-53 20.46173858946615 28.333449904734692 27.758847966817786 23.445963538981374 54-55 22.16372820219437 28.286767313473742 30.258211197521135 19.291293286810753 56-57 20.38217751139674 28.104729345779468 29.962873106412147 21.55022003641165 58-59 20.191147639707886 28.63713797034247 29.93076598157557 21.240948408374077 60-61 22.129849373449044 28.203652074510206 30.177262787908 19.48923576413275 62-63 20.195952818275238 28.414027445154012 29.822511274592827 21.567508461977923 64-65 21.984758964130492 28.427947421971655 30.161028153862922 19.42626546003493 66-67 20.273776781719473 32.031843089924514 27.871910085142975 19.822470043213038 68-69 20.111362405687743 32.483507147853366 27.842611193462012 19.562519252996886 70-71 20.064871988493728 32.17322741214513 28.069939860271937 19.69196073908921 72-73 20.0971104012531 32.21762973460597 27.805377949658666 19.879881914482255 74-75 19.94623734241261 31.96210091596094 28.248696656197552 19.842965085428897 76-77 20.226855824643135 31.456541225794098 28.02155715697155 20.295045792591218 78-79 20.09767380550647 31.584078579952614 28.148952553397372 20.16929506114354 80-81 20.08913593779001 31.10778410270159 28.371169521950723 20.431910437557672 82-83 20.19978738442297 31.22306434964714 27.98667054431799 20.590477721611897 84-85 20.02631301127684 31.00187685556356 28.48275529803408 20.48905483512552 86-87 20.30719079231484 30.568881816003028 28.32702194110875 20.796905450573377 88-89 20.2556679584748 30.97485542084192 28.205865169913913 20.56361145076937 90-91 20.28503971946951 30.45794728152712 28.438246747616287 20.818766251387082 92-93 20.347884866052578 30.621110511584344 28.110265493622528 20.920739128740554 94-95 20.121629651980065 30.536293665732252 28.476247823355987 20.865828858931703 96-97 20.32219810033084 30.318131041784856 28.206595691199848 21.153075166684452 98-99 20.222898562451803 30.619243902954903 28.18370819664621 20.97414933794709 100-101 20.344862116324133 30.29753954393717 28.23428907886022 21.123309260878482 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31.0 1 20.5 2 17.5 3 43.0 4 53.5 5 35.5 6 28.5 7 38.5 8 45.5 9 44.5 10 47.5 11 54.0 12 78.5 13 100.0 14 124.5 15 184.0 16 288.5 17 504.0 18 847.5 19 1419.0 20 2453.5 21 3985.5 22 6242.0 23 9501.5 24 13871.5 25 19607.5 26 27052.0 27 35910.0 28 46357.5 29 58698.0 30 72002.0 31 87314.0 32 106434.5 33 127992.5 34 151932.0 35 179881.5 36 211674.0 37 246121.5 38 278844.5 39 303720.5 40 319571.0 41 324278.5 42 316103.0 43 300426.0 44 281604.5 45 264283.0 46 248421.5 47 230984.5 48 211090.5 49 190313.5 50 171351.0 51 152148.0 52 131811.5 53 111331.0 54 92392.5 55 76204.5 56 62201.5 57 50447.0 58 40663.0 59 32198.5 60 25021.0 61 19113.0 62 14407.0 63 10581.5 64 7594.0 65 5455.5 66 3964.5 67 2818.5 68 1973.0 69 1392.0 70 974.5 71 709.5 72 547.0 73 423.5 74 307.5 75 216.5 76 166.0 77 132.5 78 99.5 79 71.0 80 54.0 81 48.5 82 33.0 83 21.0 84 16.0 85 12.5 86 13.0 87 7.5 88 6.0 89 4.5 90 4.5 91 4.5 92 1.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15682022294622988 2 0.0 3 0.005107407372954996 4 0.00680987649727333 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.265368425726801E-5 14-15 0.0 16-17 1.7551228085756003E-5 18-19 0.0 20-21 0.0 22-23 1.2285859660029203E-4 24-25 3.5102456171512006E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0011583810536598962 40-41 0.0 42-43 0.0 44-45 1.1408298255741401E-4 46-47 0.0011408298255741402 48-49 0.0031504454413932024 50-51 4.387807021439001E-4 52-53 0.0017112447383612103 54-55 0.0027818696515923263 56-57 0.0044667875478249025 58-59 0.005888437022771139 60-61 0.0018516545630472584 62-63 0.001088176141316872 64-65 0.0016498154400610642 66-67 0.008494794393505905 68-69 0.007757642813904153 70-71 0.009495214394393997 72-73 0.00666946667258728 74-75 0.009302150885450682 76-77 0.0050547536886977286 78-79 0.004844138951668657 80-81 0.0022728840371054023 82-83 0.0 84-85 7.985808779018981E-4 86-87 0.0018516545630472584 88-89 0.005186387899340898 90-91 0.007038042462388158 92-93 0.009144189832678878 94-95 0.008363160182862735 96-97 0.010794005272739942 98-99 0.007748867199861275 100-101 0.007573354919003714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5697607.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.93295746774587 #Duplication Level Percentage of deduplicated Percentage of total 1 96.97547613511315 85.2734041840331 2 2.0571092444280406 3.6177539939359544 3 0.3738786452607952 0.9862876503544784 4 0.17452810650555264 0.6138709026511593 5 0.07945115919188096 0.34931877009913875 6 0.06397289437016812 0.33751954798443545 7 0.0587935022936161 0.36189205755950543 8 0.03775336189121348 0.26558118123555935 9 0.024424020371525225 0.19329087100686174 >10 0.13465629432558682 2.2711736992287905 >50 0.011000752789271652 0.6701465307742394 >100 0.008751328379868537 1.29629570897723 >500 6.628618833571585E-5 0.037768084162303185 >1k 1.1831668238533374E-4 0.1444997818216297 >5k 0.0 0.0 >10k+ 1.9952208596055734E-5 3.5811970361755443 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 204120 3.582556676864515 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014918543872892603 0.0 0.0 2 0.0016849178962325762 0.0 0.0 3 0.0019306350894331603 0.0 0.0 4 0.0021939035107195 0.0 0.0 5 0.0024922743881773523 0.0 0.0 6 0.0029661575464927643 0.0 0.0 7 0.0037033091260945165 0.0 1.7551228085756003E-5 8 0.004651075442725341 0.0 1.7551228085756003E-5 9 0.005212714741469533 0.0 1.7551228085756003E-5 10-11 0.007108247374731181 0.0 1.7551228085756003E-5 12-13 0.008907248253521172 8.775614042878002E-6 2.6326842128634003E-5 14-15 0.010723800360396918 1.7551228085756003E-5 3.5102456171512006E-5 16-17 0.012575454923444175 1.7551228085756003E-5 3.5102456171512006E-5 18-19 0.014593846153306115 1.7551228085756003E-5 3.5102456171512006E-5 20-21 0.017761842822785076 1.7551228085756003E-5 6.142929830014602E-5 22-23 0.021131678615250227 1.7551228085756003E-5 8.775614042878002E-5 24-25 0.0247735584430446 1.7551228085756003E-5 1.3163421064317E-4 26-27 0.028073189323166728 1.7551228085756003E-5 1.4040982468604802E-4 28-29 0.031855478975647146 1.7551228085756003E-5 1.6673666681468204E-4 30-31 0.04786219898985662 1.7551228085756003E-5 1.9306350894331603E-4 32-33 0.05311879180154054 1.7551228085756003E-5 2.1061473702907202E-4 34-35 0.05791027706895193 1.7551228085756003E-5 2.2816596511482804E-4 36-37 0.06348279198617947 1.7551228085756003E-5 2.4571719320058406E-4 38-39 0.0696871511144942 1.7551228085756003E-5 2.6326842128634006E-4 40-41 0.07556681252322248 1.7551228085756003E-5 2.7204403532921805E-4 42-43 0.08256097691539624 1.7551228085756003E-5 2.9837087745785204E-4 44-45 0.09009045376418556 1.7551228085756003E-5 2.9837087745785204E-4 46-47 0.09772523798148942 1.7551228085756003E-5 3.1592210554360803E-4 48-49 0.10554431009369372 1.7551228085756003E-5 3.3347333362936403E-4 50-51 0.11528524168128831 1.7551228085756003E-5 3.7735140384375406E-4 52-53 0.1256492418659272 1.7551228085756003E-5 4.0367824597238805E-4 54-55 0.1362765104718525 1.7551228085756003E-5 4.2122947405814405E-4 56-57 0.14777256486802265 1.7551228085756003E-5 4.2122947405814405E-4 58-59 0.1602778148791238 3.5102456171512006E-5 4.5633193022965603E-4 60-61 0.1739589971719706 3.5102456171512006E-5 4.738831583154121E-4 62-63 0.18902672648359214 3.5102456171512006E-5 4.914343864011681E-4 64-65 0.20410323140925657 3.5102456171512006E-5 5.177612285298021E-4 66-67 0.22113669826648275 3.5102456171512006E-5 5.528636847013141E-4 68-69 0.24067121512592918 3.5102456171512006E-5 5.616392987441921E-4 70-71 0.26242596233822374 3.5102456171512006E-5 5.791905268299481E-4 72-73 0.2865325741140096 3.5102456171512006E-5 6.055173689585821E-4 74-75 0.31308758220775845 3.5102456171512006E-5 6.318442110872161E-4 76-77 0.3430299773220582 3.5102456171512006E-5 6.406198251300941E-4 78-79 0.3762369008603086 3.5102456171512006E-5 6.493954391729721E-4 80-81 0.41343672878806836 5.2653684257268006E-5 6.493954391729721E-4 82-83 0.45448905128065165 5.2653684257268006E-5 6.581710532158501E-4 84-85 0.5010524593921624 5.2653684257268006E-5 6.84497895344484E-4 86-87 0.5533287922455866 5.2653684257268006E-5 7.371515796017521E-4 88-89 0.6123447966839412 5.2653684257268006E-5 7.459271936446301E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27305 0.0 77.78073 3 GATCGGA 27785 0.0 76.62108 1 CGGAAGA 27675 0.0 76.583 4 ATCGGAA 27775 0.0 76.49875 2 GAAGAGC 30160 0.0 70.28508 6 AGAGCAC 31400 0.0 67.38848 8 GAGCACA 31475 0.0 67.30335 9 AAGAGCA 34335 0.0 61.65569 7 GGAAGAG 37025 0.0 57.548203 5 GCCGTCT 23340 0.0 45.038345 50-51 ATGCCGT 23380 0.0 44.747574 48-49 TGCCGTC 23395 0.0 44.729427 50-51 CGTATGC 23425 0.0 44.691635 46-47 TATGCCG 23345 0.0 44.662056 48-49 TCTCGTA 23425 0.0 44.66122 42-43 CTCGTAT 23485 0.0 44.395424 44-45 TCGTATG 23870 0.0 44.31615 44-45 CGTCTTC 23800 0.0 44.257477 52-53 ATCTCGT 23580 0.0 44.246777 42-43 CCGTCTT 23695 0.0 44.192986 52-53 >>END_MODULE