##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2365610 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67167157730987 33.0 33.0 33.0 33.0 33.0 2 32.70907376955627 33.0 33.0 33.0 33.0 33.0 3 32.74214473222551 33.0 33.0 33.0 33.0 33.0 4 36.67683219127413 37.0 37.0 37.0 37.0 37.0 5 36.67941926183944 37.0 37.0 37.0 37.0 37.0 6 36.546152577982 37.0 37.0 37.0 37.0 37.0 7 36.483454161928634 37.0 37.0 37.0 37.0 37.0 8 36.41882727922185 37.0 37.0 37.0 37.0 37.0 9 36.43432518462468 37.0 37.0 37.0 37.0 37.0 10-11 36.3829061003293 37.0 37.0 37.0 37.0 37.0 12-13 36.36886088577576 37.0 37.0 37.0 37.0 37.0 14-15 38.19496430096254 40.0 37.0 40.0 37.0 40.0 16-17 38.164929553053966 40.0 37.0 40.0 37.0 40.0 18-19 38.190668368835105 40.0 37.0 40.0 37.0 40.0 20-21 38.14567997260749 40.0 37.0 40.0 37.0 40.0 22-23 38.057754448112746 40.0 37.0 40.0 37.0 40.0 24-25 38.01233339392377 40.0 37.0 40.0 35.0 40.0 26-27 37.90304509196359 40.0 37.0 40.0 33.0 40.0 28-29 37.79261797168595 40.0 37.0 40.0 33.0 40.0 30-31 37.76132646547825 40.0 37.0 40.0 33.0 40.0 32-33 37.6246834854435 40.0 37.0 40.0 33.0 40.0 34-35 37.54953732863828 40.0 37.0 40.0 33.0 40.0 36-37 37.46574667844658 40.0 37.0 40.0 33.0 40.0 38-39 37.354764521624446 40.0 37.0 40.0 33.0 40.0 40-41 37.29402078110931 40.0 37.0 40.0 33.0 40.0 42-43 37.15585092217229 40.0 37.0 40.0 33.0 40.0 44-45 37.028973076711715 37.0 37.0 40.0 33.0 40.0 46-47 36.98091316827372 37.0 37.0 40.0 33.0 40.0 48-49 36.77217377336078 37.0 37.0 40.0 33.0 40.0 50-51 36.594474152544166 37.0 37.0 40.0 33.0 40.0 52-53 36.44153009160428 37.0 37.0 40.0 33.0 40.0 54-55 36.28297732931464 37.0 37.0 40.0 33.0 40.0 56-57 36.10722097048964 37.0 37.0 40.0 33.0 40.0 58-59 35.85115128867396 37.0 37.0 40.0 27.0 40.0 60-61 35.576737923833605 37.0 33.0 40.0 27.0 40.0 62-63 35.315320784068376 37.0 33.0 40.0 27.0 40.0 64-65 35.02653839812987 37.0 33.0 37.0 27.0 40.0 66-67 34.690875926293856 37.0 33.0 37.0 27.0 40.0 68-69 34.40332852837112 37.0 33.0 37.0 27.0 40.0 70-71 34.00836951145793 37.0 33.0 37.0 27.0 40.0 72-73 33.62091299918414 37.0 33.0 37.0 27.0 37.0 74-75 33.21915150849041 37.0 33.0 37.0 27.0 37.0 76-77 30.545821162406313 33.0 30.0 35.0 24.5 37.0 78-79 32.99345982642954 35.0 33.0 37.0 27.0 37.0 80-81 33.41862056721099 37.0 33.0 37.0 27.0 37.0 82-83 33.379746450175645 37.0 33.0 37.0 27.0 37.0 84-85 33.211366624253365 37.0 33.0 37.0 27.0 37.0 86-87 33.061107283110914 37.0 33.0 37.0 27.0 37.0 88-89 32.92390799835983 37.0 33.0 37.0 27.0 37.0 90-91 32.76795752469765 33.0 33.0 37.0 27.0 37.0 92-93 32.62846115800998 33.0 33.0 37.0 27.0 37.0 94-95 32.44971444997273 33.0 33.0 37.0 24.5 37.0 96-97 32.2934963920511 33.0 33.0 37.0 22.0 37.0 98-99 32.15309856654309 33.0 33.0 37.0 22.0 37.0 100-101 30.577132325277624 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19074036246169612 2306 2 0.1637043799332787 2306 3 0.16093486897710108 2306 4 0.17212060404741436 2306 5 0.14203114660040939 2306 6 0.20627391598964806 2306 7 0.14790196285311197 2306 8 0.25568417644181807 2306 9 0.2627308030778224 2306 10-11 0.29031753719476683 2306 12-13 0.21093846433165453 2306 14-15 0.5374777207632064 2306 16-17 0.4771096859871804 2306 18-19 0.5040665118067338 2306 20-21 0.5099963237135796 2306 22-23 0.5308002720209686 2306 24-25 0.5124676773701182 2306 26-27 0.5567430503881425 2306 28-29 0.49474326958738146 2306 30-31 0.44054989134343003 2306 32-33 0.4727097364453954 2306 34-35 0.5418260527252343 2306 36-37 0.3816762971933443 2306 38-39 0.48756863966228536 2306 40-41 0.3358850491926546 2306 42-43 0.4072774372455399 2306 44-45 0.6007969396225548 2306 46-47 0.4851947369678129 2306 48-49 0.4697906873542834 2306 50-51 0.45027157954759645 2306 52-53 0.5492601470009788 2306 54-55 0.5730317164725847 2306 56-57 0.5019609999738819 2306 58-59 0.5664173316599133 2306 60-61 0.745631195296383 2306 62-63 0.4276392976186898 2306 64-65 0.5880704829730803 2306 66-67 0.586215318251817 2306 68-69 0.420992753124132 2306 70-71 0.4894246648802181 2306 72-73 0.4213955445485169 2306 74-75 0.36311080860724587 2306 76-77 -0.05217954021022564 2306 78-79 0.2723504746474319 2306 80-81 0.4362326132375429 2306 82-83 0.47394861374946373 2306 84-85 0.536551460042503 2306 86-87 0.44699103017899233 2306 88-89 0.4267830683211926 2306 90-91 0.4111888311834164 2306 92-93 0.5077979415460021 2306 94-95 0.40990549585729497 2306 96-97 0.3799315763772384 2306 98-99 0.5420294465314086 2306 100-101 0.3149526817755941 2307 1 0.0757712391728873 2307 2 0.0846565755404427 2307 3 0.10386566081562876 2307 4 0.10592623303313786 2307 5 0.11628103262597023 2307 6 0.19358085770861777 2307 7 0.08723782975259553 2307 8 0.18951953788944564 2307 9 0.1254271949985224 2307 10-11 0.14810266699949182 2307 12-13 0.19492618212193946 2307 14-15 0.35174759060710414 2307 16-17 0.35698423301762716 2307 18-19 0.28683438777371606 2307 20-21 0.2921375655213865 2307 22-23 0.35635000722185595 2307 24-25 0.32632444619031276 2307 26-27 0.35552456004037936 2307 28-29 0.3780983166277352 2307 30-31 0.47185682225059367 2307 32-33 0.46371386475268395 2307 34-35 0.36939354090883825 2307 36-37 0.273997032972666 2307 38-39 0.3951596975793237 2307 40-41 0.20174344338742856 2307 42-43 0.016110861578219726 2307 44-45 0.1987856737224476 2307 46-47 0.21718317194111592 2307 48-49 0.27683151452938404 2307 50-51 0.284257225075379 2307 52-53 0.05040529727605758 2307 54-55 0.10682085745671088 2307 56-57 0.01706689258742955 2307 58-59 0.06896621453022789 2307 60-61 0.18370703649980413 2307 62-63 0.09028127012427234 2307 64-65 0.028924957888797564 2307 66-67 0.1097008097113985 2307 68-69 0.18088014818205522 2307 70-71 0.07345130516608833 2307 72-73 0.013909901723678786 2307 74-75 -0.1257800624457701 2307 76-77 -0.46556392327966023 2307 78-79 -0.1729278302058077 2307 80-81 0.08585762382410422 2307 82-83 0.057358530696788534 2307 84-85 0.06814715622358847 2307 86-87 -0.19032474118017717 2307 88-89 -0.1085958583909914 2307 90-91 -0.06372192638169594 2307 92-93 -0.2802569067373497 2307 94-95 -0.02413934429919351 2307 96-97 0.10412334895453768 2307 98-99 -0.04863113669335206 2307 100-101 -0.20911124121553115 2201 1 0.11103357253204393 2201 2 0.14652268954361602 2201 3 0.0638747559451005 2201 4 0.13584609998920882 2201 5 0.11694709540976334 2201 6 0.10734733209181258 2201 7 0.17440484446868254 2201 8 0.11861019883556168 2201 9 0.04511419818497586 2201 10-11 0.10867138133495757 2201 12-13 0.15974210060091565 2201 14-15 0.3704896962613091 2201 16-17 0.3155950705546928 2201 18-19 0.1611878879494526 2201 20-21 0.1499039155447548 2201 22-23 0.19162040289160132 2201 24-25 0.1580811498961836 2201 26-27 0.22938127960739507 2201 28-29 0.2283867418371628 2201 30-31 0.271626045196669 2201 32-33 0.3179948462889328 2201 34-35 0.22482406039038239 2201 36-37 0.22356517240744012 2201 38-39 0.3506943420656725 2201 40-41 0.29951621691185437 2201 42-43 0.31370760798799324 2201 44-45 0.30780273578483985 2201 46-47 0.20549690542853227 2201 48-49 0.23769400260059825 2201 50-51 0.3667641715727257 2201 52-53 0.2246228711107463 2201 54-55 0.2369985866147104 2201 56-57 0.2890771322488348 2201 58-59 0.23873950551799084 2201 60-61 0.3941677264122774 2201 62-63 0.2645141254627035 2201 64-65 0.07085753589428379 2201 66-67 0.1408852211021241 2201 68-69 0.19556448764432588 2201 70-71 0.16554871263262783 2201 72-73 0.2685881496623921 2201 74-75 0.16123766778721205 2201 76-77 0.05057347640912013 2201 78-79 0.17516756747951945 2201 80-81 0.34569021758535 2201 82-83 0.411288301121175 2201 84-85 0.2413016116513944 2201 86-87 0.16314052422529102 2201 88-89 0.2866755174027915 2201 90-91 0.1973421458018052 2201 92-93 0.17104660553184203 2201 94-95 0.2134573441462564 2201 96-97 0.3741398793260018 2201 98-99 0.3054062629427321 2201 100-101 0.39067131352518913 1101 1 0.06177165201282975 1101 2 0.06394782590115966 1101 3 0.0212509462554209 1101 4 0.15288369511502253 1101 5 0.14912132005294154 1101 6 0.07621878167829266 1101 7 0.16973187592289207 1101 8 0.008816313321979408 1101 9 0.08211956659965125 1101 10-11 0.09215232897395254 1101 12-13 0.15582577267098685 1101 14-15 0.17500742405391634 1101 16-17 0.19947835107547007 1101 18-19 0.11879287168579111 1101 20-21 0.14781483354796165 1101 22-23 0.24410606811267144 1101 24-25 0.25919508043364203 1101 26-27 0.22692312208395293 1101 28-29 0.1533724392658442 1101 30-31 0.2678774354924869 1101 32-33 0.32869004429903015 1101 34-35 0.3498583555467789 1101 36-37 0.09739370950847359 1101 38-39 0.18038793542030618 1101 40-41 0.19109330264187463 1101 42-43 0.16416378724751723 1101 44-45 0.26691658711539645 1101 46-47 0.2400482913822799 1101 48-49 0.300480978505 1101 50-51 0.19426633425511852 1101 52-53 0.19099834903612134 1101 54-55 0.2704315329634852 1101 56-57 0.3269967200675765 1101 58-59 0.19246466329956746 1101 60-61 0.24532185683741403 1101 62-63 0.13033906771563153 1101 64-65 0.145375602463389 1101 66-67 0.01636791797302095 1101 68-69 0.043347195441960196 1101 70-71 0.003085459147172287 1101 72-73 -0.09222051461820513 1101 74-75 0.014576787359565913 1101 76-77 -0.08292343189744145 1101 78-79 0.0929064299634561 1101 80-81 0.3259728287601078 1101 82-83 0.38221555149180375 1101 84-85 0.27224669788910205 1101 86-87 0.23526496780873885 1101 88-89 0.19218037311978975 1101 90-91 0.17385539124727956 1101 92-93 0.1533433861530682 1101 94-95 0.0862360319551172 1101 96-97 0.14220680629389193 1101 98-99 0.11727198148053475 1101 100-101 0.10448151375164372 2304 1 0.11897570907716215 2304 2 0.10901404639602674 2304 3 0.09731912821474253 2304 4 0.10408483704041771 2304 5 0.09950762475504149 2304 6 0.08036660033641851 2304 7 0.06399332822275028 2304 8 0.10077493005928062 2304 9 0.039235264613964205 2304 10-11 0.07753272089424001 2304 12-13 0.09071242734945173 2304 14-15 0.18754003750347437 2304 16-17 0.20732243461203836 2304 18-19 0.1428287954947436 2304 20-21 0.11702467653321946 2304 22-23 0.13908144556815216 2304 24-25 0.20035757858173753 2304 26-27 0.13705926244355027 2304 28-29 0.13439697841928222 2304 30-31 0.06792782499088901 2304 32-33 0.27129214735087714 2304 34-35 0.21233627687351486 2304 36-37 0.2543363710498383 2304 38-39 0.19471744902858035 2304 40-41 0.14989656092620862 2304 42-43 0.07574485534520647 2304 44-45 0.12859586150662494 2304 46-47 0.13503653235409274 2304 48-49 0.20295401169553173 2304 50-51 0.11828420478426693 2304 52-53 0.1634730855336386 2304 54-55 0.09917776594926409 2304 56-57 0.11613463381814171 2304 58-59 0.14555829461699687 2304 60-61 0.1639550150790754 2304 62-63 0.2394130364830076 2304 64-65 0.2035337775828694 2304 66-67 0.03574835779882335 2304 68-69 0.0045428459765943785 2304 70-71 -0.018493089475590807 2304 72-73 0.033342042435918984 2304 74-75 -0.06165573710994465 2304 76-77 -0.46386826160666317 2304 78-79 0.14748389006658869 2304 80-81 0.2792509850012621 2304 82-83 0.3701249550770882 2304 84-85 0.39213267390618967 2304 86-87 0.2725134971539731 2304 88-89 0.23008139324098664 2304 90-91 0.22601406671318358 2304 92-93 0.16957448232869865 2304 94-95 0.18225131381250748 2304 96-97 0.20445469246330816 2304 98-99 0.26641070252764365 2304 100-101 0.2090539171621586 2202 1 0.04356066987315188 2202 2 0.06531662237579638 2202 3 0.017250449773762 2202 4 0.01991731265228225 2202 5 0.019691501468379613 2202 6 0.02685140521064966 2202 7 -0.022426491312096175 2202 8 0.05079592111882647 2202 9 0.08491835178192986 2202 10-11 0.0673193490510755 2202 12-13 0.08820944016020604 2202 14-15 0.13840253405671632 2202 16-17 0.023681094397055347 2202 18-19 -0.03879665775895802 2202 20-21 -0.05515003863839496 2202 22-23 -0.041023915473928696 2202 24-25 -0.05110682728847138 2202 26-27 0.13311629684984183 2202 28-29 0.15049488535809274 2202 30-31 0.030981904087852286 2202 32-33 -0.07193051438171949 2202 34-35 -0.14185183282982194 2202 36-37 -0.19614087538685254 2202 38-39 -0.13626125601764016 2202 40-41 0.01803689381873852 2202 42-43 -0.22134011994165803 2202 44-45 -0.2005135213727911 2202 46-47 -0.2861341725728863 2202 48-49 -0.31115491989680066 2202 50-51 -0.15006040796588138 2202 52-53 -0.06660880029983218 2202 54-55 -0.22406550594001828 2202 56-57 -0.2578066011517208 2202 58-59 -0.30462002588294723 2202 60-61 -0.18417384104481727 2202 62-63 -0.2043019421905754 2202 64-65 -0.4295275966318357 2202 66-67 -0.2776568557676171 2202 68-69 -0.29129446544543924 2202 70-71 -0.038781962889153476 2202 72-73 -0.18871914712369176 2202 74-75 -0.1741359363718189 2202 76-77 -0.383795442917247 2202 78-79 -0.23295132456802747 2202 80-81 -0.09282486103780485 2202 82-83 0.06983853269059637 2202 84-85 0.06914837455629197 2202 86-87 0.14609660063451457 2202 88-89 0.0018686225618935737 2202 90-91 0.005665283904825458 2202 92-93 -0.1726944731777067 2202 94-95 -0.15813341463273645 2202 96-97 0.012559559987785462 2202 98-99 -0.13502468020106306 2202 100-101 -0.06388761342143923 1102 1 0.0924988684508321 1102 2 0.0709442104239244 1102 3 0.07394878998926657 1102 4 0.03424255572095092 1102 5 0.03391006863157031 1102 6 0.02919133058767187 1102 7 0.07062865646585692 1102 8 -0.11152757655455758 1102 9 -0.016408904818568715 1102 10-11 0.025097874438984036 1102 12-13 -0.02240016344911311 1102 14-15 -0.150951905030567 1102 16-17 -0.08918972976208295 1102 18-19 -0.1556550002584416 1102 20-21 -0.2322125584351582 1102 22-23 -0.22871988181294967 1102 24-25 -0.11151172118548658 1102 26-27 -0.015154820446198869 1102 28-29 -0.1490554940261788 1102 30-31 -0.24473219754091247 1102 32-33 -0.12563402018022174 1102 34-35 -0.2139607904036751 1102 36-37 -0.08918333008238477 1102 38-39 -0.14233618841443274 1102 40-41 -0.1108483371818636 1102 42-43 -0.2700012119402686 1102 44-45 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quality scores pass #Quality Count 8 6.0 9 22.0 10 25.0 11 20.0 12 19.0 13 67.0 14 187.0 15 421.0 16 894.0 17 1483.0 18 2258.0 19 3141.0 20 4072.0 21 5409.0 22 6969.0 23 9082.0 24 11380.0 25 14649.0 26 18766.0 27 24785.0 28 34681.0 29 47016.0 30 49678.0 31 56426.0 32 74815.0 33 106783.0 34 162208.0 35 279292.0 36 534935.0 37 752901.0 38 163146.0 39 74.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.66698502933424 25.502712432993548 24.741012816637497 15.089289721034712 2 28.707817433981088 23.393923765963113 34.05413402885514 13.844124771200663 3 36.83027504707278 19.47557499811878 26.818674893869588 16.875475060938854 4 27.861957416873956 19.289782383349596 34.726926530494914 18.12133366928154 5 32.2406905618424 18.146820481820757 32.77695816301081 16.835530793326033 6 25.740168497765904 19.37225493635891 38.126614277078644 16.760962288796545 7 31.4906091874823 20.845109718000852 29.67095167842544 17.99332941609141 8 27.467460824058065 28.16005174141131 26.67324706946623 17.6992403650644 9 30.3051644184798 24.52128626443074 25.97638664023233 19.197162676857133 10-11 24.241485282865728 28.79973875659978 28.998693782998885 17.960082177535604 12-13 23.658172372213055 26.89609328681956 29.014322959324403 20.43141138164298 14-15 22.966570990146305 27.434276994094546 29.77289155862547 19.826260457133678 16-17 22.64451874992074 27.62667979929067 30.62704756912593 19.10175388166266 18-19 20.896808856912173 27.933070117221355 31.49902139405904 19.67109963180744 20-21 21.794928158064938 28.094254758814852 31.006886173122368 19.103930909997842 22-23 21.116099264247413 29.538659243618444 30.267098691455253 19.078142800678897 24-25 21.491113489639872 27.39479660197294 31.19699037203121 19.91709953635598 26-27 21.372817159210523 27.562552576291104 30.26060931429948 20.804020950198893 28-29 21.366793342943257 28.78521818896606 30.040539226668812 19.807449241421875 30-31 20.16600369460731 28.716441002532118 30.521028402822104 20.596526900038466 32-33 19.966942987221056 29.50778023427361 30.40503295133179 20.12024382717354 34-35 21.03110825537599 28.93080431685696 30.643597211712837 19.394490216054212 36-37 20.16243167724181 29.39034752135813 31.266607767129834 19.18061303427023 38-39 20.724072452054283 29.7654456051593 29.723067206855617 19.787414735930795 40-41 20.615063345183696 28.58545998706465 31.11087626447301 19.68860040327865 42-43 20.589129230938322 29.48309315567655 30.79694032406018 19.130837289324955 44-45 20.116359264619255 28.86663852727707 31.189617706594657 19.82738450150902 46-47 21.10913273626995 28.77683593607989 30.28145763952827 19.832573688121887 48-49 20.733487702107016 30.139809071684247 30.015419263405196 19.111283962803537 50-51 21.35557102266302 29.336443476024094 29.68394612176606 19.624039379546822 52-53 20.197103059333195 28.941569760621622 29.43578583462848 21.425541345416708 54-55 21.388093270878816 29.121690558457264 29.80869446852259 19.681521702141332 56-57 20.71197664100768 28.991935364548834 29.448432538734725 20.84765545570876 58-59 20.55731887654376 28.836061595798917 30.224782560539353 20.381836967117962 60-61 20.818093025605407 29.156680544197616 30.334914415081833 19.690312015115143 62-63 20.224019939186498 29.260654728011115 29.944648128397404 20.570677204404983 64-65 21.16354900475845 28.981225123716936 29.816664528362264 20.038561343162346 66-67 20.839734608825346 30.18302504339621 28.890143543582646 20.0870968041958 68-69 21.046343656584384 30.29727699363796 28.724776859413293 19.93160249036436 70-71 20.576821939027443 31.1560409437421 28.753902101638968 19.513235015591494 72-73 20.212030192795076 31.482879687503303 28.628916513243695 19.67617360645793 74-75 20.88778050434926 30.600685844640946 28.572174442045746 19.939359208964046 76-77 21.293669614101184 29.63588935717068 29.099534200646925 19.97090682808121 78-79 21.142296344927324 29.473216523984075 29.687441212391253 19.697045918697345 80-81 20.33532249156494 30.091793808139972 29.42865651817625 20.14422718211884 82-83 19.548192643757847 29.985669658143145 29.66251833565127 20.80361936244774 84-85 19.64549426816455 29.496363190196494 30.10548893760091 20.75265360403804 86-87 19.68133450344608 29.717122706274644 30.721229937300894 19.880312852978378 88-89 19.952513140476004 30.148660122726877 30.27370786640352 19.625118870393592 90-91 20.34090298982901 29.649521677243218 29.68917581537236 20.320399517555412 92-93 19.680400980552353 30.48142144156077 29.595250531463684 20.242927046423198 94-95 19.29262774653005 30.969915868938934 29.525032906467004 20.21242347806401 96-97 19.82256037961112 31.315824852623354 29.057127685840005 19.80448708192552 98-99 20.2741007607874 30.450393245411856 28.711206014600222 20.564299979200523 100-101 20.21869531930041 30.344223921414944 28.33814639747328 21.09893436181137 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 120.0 1 76.0 2 72.0 3 157.5 4 166.5 5 109.5 6 86.0 7 69.0 8 70.5 9 82.0 10 75.5 11 65.5 12 63.5 13 76.5 14 81.5 15 79.0 16 82.0 17 99.5 18 132.5 19 190.5 20 295.5 21 458.0 22 686.0 23 1034.5 24 1542.5 25 2341.5 26 3521.5 27 5070.0 28 6951.0 29 8871.0 30 11009.5 31 13683.5 32 19272.5 33 29680.0 34 45563.5 35 68541.5 36 101428.0 37 142772.0 38 183118.0 39 200944.5 40 198408.5 41 178976.0 42 152597.5 43 143371.0 44 135029.5 45 124707.0 46 122549.5 47 108866.0 48 80799.5 49 60499.0 50 48673.5 51 39677.5 52 32674.5 53 25725.5 54 19048.0 55 13411.0 56 9279.5 57 6199.0 58 4385.5 59 3293.0 60 2457.0 61 1828.0 62 1311.5 63 895.5 64 620.5 65 468.5 66 317.0 67 210.5 68 175.0 69 127.5 70 91.0 71 67.0 72 54.5 73 40.0 74 25.0 75 24.5 76 17.5 77 7.0 78 5.0 79 4.5 80 3.0 81 2.5 82 2.0 83 1.5 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14896791947954227 2 0.0 3 0.00524177696239025 4 0.007017217546425657 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.0568098714496473E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.1136197428992947E-4 24-25 8.454478971597178E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.60903107443746E-4 40-41 0.0 42-43 0.0 44-45 2.324981717189224E-4 46-47 0.0013527166354555485 48-49 0.002642024678624118 50-51 6.974945151567672E-4 52-53 0.0019233939660383578 54-55 0.0020502111506123157 56-57 0.0041004223012246314 58-59 0.00524177696239025 60-61 0.0015006700174584992 62-63 7.186307125857601E-4 64-65 0.0012047632534525979 66-67 0.008285389392165235 68-69 0.007313124310431558 70-71 0.009215382079040924 72-73 0.006129497254407954 74-75 0.0086869771433161 76-77 0.004502010052375498 78-79 0.004354056670372547 80-81 0.0015429424123164848 82-83 0.0 84-85 2.7477056657690825E-4 86-87 0.001099082266307633 88-89 0.004797916816381398 90-91 0.006636765992703785 92-93 0.008813794327890057 94-95 0.00817970840502027 96-97 0.010229919555632586 98-99 0.007017217546425657 100-101 0.007313124310431558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2365610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.802429820650524 #Duplication Level Percentage of deduplicated Percentage of total 1 87.92546084241704 35.87572445471076 2 5.298029553110934 4.323449580570825 3 1.7283787983824728 2.115661638735034 4 1.0317504076241337 1.6839169439804518 5 0.6826359037283682 1.3926601777466543 6 0.4577110188513822 1.1205433034893182 7 0.38957324044395614 1.112687436225255 8 0.25384334181073415 0.8285940111737506 9 0.1954050205480297 0.717569967376238 >10 1.5992209659674004 12.874194621967455 >50 0.20515979462910144 5.928503889068043 >100 0.194206086776055 17.34535836009438 >500 0.031103791339984404 8.473071458496486 >1k 0.007417353486690263 4.770884607457961 >5k 0.0 0.0 >10k+ 1.0388088378410848E-4 1.4371795489074144 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 33907 1.4333300924497276 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 4977 0.2103897092081958 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3933 0.1662573289764585 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3888 0.16435507120784915 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3736 0.1579296671894353 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3125 0.1321012339312059 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3035 0.12829671839398718 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2825 0.11941951547381013 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2684 0.11345910779883414 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.031755023017319E-4 0.0 0.0 2 8.877202920177037E-4 0.0 0.0 3 0.0010568098714496472 0.0 0.0 4 0.001141354661165619 0.0 4.227239485798589E-5 5 0.0016063510046034639 0.0 4.227239485798589E-5 6 0.0017331681891774215 0.0 4.227239485798589E-5 7 0.0018177129788933932 0.0 4.227239485798589E-5 8 0.0019868025583253367 0.0 4.227239485798589E-5 9 0.002240436927473252 0.0 4.227239485798589E-5 10-11 0.0033183829963518924 0.0 4.227239485798589E-5 12-13 0.004290648078085568 0.0 4.227239485798589E-5 14-15 0.006129497254407954 0.0 8.454478971597178E-5 16-17 0.007926074035872354 0.0 8.454478971597178E-5 18-19 0.00997628518648467 0.0 8.454478971597178E-5 20-21 0.012068768731954973 0.0 8.454478971597178E-5 22-23 0.014901019187440027 0.0 8.454478971597178E-5 24-25 0.018346219368365876 0.0 8.454478971597178E-5 26-27 0.021580057575001796 0.0 8.454478971597178E-5 28-29 0.02525775592764657 0.0 8.454478971597178E-5 30-31 0.039144237638494936 0.0 8.454478971597178E-5 32-33 0.043477158111438485 0.0 8.454478971597178E-5 34-35 0.04726053745122823 0.0 8.454478971597178E-5 36-37 0.05184709229331969 0.0 8.454478971597178E-5 38-39 0.0563279661482662 0.0 8.454478971597178E-5 40-41 0.06182337747980436 0.0 8.454478971597178E-5 42-43 0.06714969923191058 0.0 8.454478971597178E-5 44-45 0.07319465169660258 0.0 8.454478971597178E-5 46-47 0.07938755754329749 0.0 8.454478971597178E-5 48-49 0.08613000452314626 0.0 8.454478971597178E-5 50-51 0.09238631896212816 0.0 8.454478971597178E-5 52-53 0.09910762974454793 0.0 8.454478971597178E-5 54-55 0.10792142407243797 0.0 8.454478971597178E-5 56-57 0.11673521840032802 0.0 8.454478971597178E-5 58-59 0.12685945696881565 0.0 8.454478971597178E-5 60-61 0.13761778146017306 0.0 8.454478971597178E-5 62-63 0.1488622384923973 0.0 8.454478971597178E-5 64-65 0.16006442312976357 0.0 8.454478971597178E-5 66-67 0.1723234176385795 0.0 8.454478971597178E-5 68-69 0.18644239752114677 0.0 8.454478971597178E-5 70-71 0.2018929578417406 0.0 1.0568098714496473E-4 72-73 0.21814669366463618 0.0 1.2681718457395767E-4 74-75 0.2360913252818512 0.0 1.2681718457395767E-4 76-77 0.2565934367879743 0.0 1.2681718457395767E-4 78-79 0.2799700711444405 0.0 1.2681718457395767E-4 80-81 0.3053123718618031 0.0 1.2681718457395767E-4 82-83 0.3327048837297779 0.0 1.2681718457395767E-4 84-85 0.36413440930669044 0.0 1.2681718457395767E-4 86-87 0.39968549338225656 0.0 1.2681718457395767E-4 88-89 0.4389776844027545 0.0 1.2681718457395767E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCC 40 8.96398E-8 71.36573 1 CCGCGAT 20 0.0020610387 71.36573 1 TCGGAAG 5450 0.0 69.2102 3 CGGAAGA 5465 0.0 68.5769 4 GATCGGA 5570 0.0 67.73551 1 ATCGGAA 5705 0.0 66.11666 2 GAAGAGC 5745 0.0 64.98243 6 GGAAGAG 6455 0.0 58.202774 5 CGGGTCG 25 0.004989775 57.092583 1 CGGCGCC 25 0.004989775 57.092583 1 CGCGGGT 25 0.0050195297 57.006886 3 GCGCAAC 50 4.2403735E-7 57.006886 2 GCGCGGG 75 2.3537723E-9 50.74896 1 TTGTGTT 25890 0.0 50.6828 1 GGCGCCG 60 1.4763591E-6 47.577152 1 ACGAGGC 65 2.5617755E-6 43.917374 1 TCGTATG 4335 0.0 43.225468 44-45 GCCGTCT 4330 0.0 43.165684 50-51 ATGCCGT 4325 0.0 43.160683 48-49 CGTATGC 4295 0.0 42.964493 46-47 >>END_MODULE