FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365610
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT339071.4333300924497276TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC49770.2103897092081958No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG39330.1662573289764585No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38880.16435507120784915No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37360.1579296671894353No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA31250.1321012339312059No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30350.12829671839398718No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28250.11941951547381013No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC26840.11345910779883414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC408.96398E-871.365731
CCGCGAT200.002061038771.365731
TCGGAAG54500.069.21023
CGGAAGA54650.068.57694
GATCGGA55700.067.735511
ATCGGAA57050.066.116662
GAAGAGC57450.064.982436
GGAAGAG64550.058.2027745
CGGGTCG250.00498977557.0925831
CGGCGCC250.00498977557.0925831
CGCGGGT250.005019529757.0068863
GCGCAAC504.2403735E-757.0068862
GCGCGGG752.3537723E-950.748961
TTGTGTT258900.050.68281
GGCGCCG601.4763591E-647.5771521
ACGAGGC652.5617755E-643.9173741
TCGTATG43350.043.22546844-45
GCCGTCT43300.043.16568450-51
ATGCCGT43250.043.16068348-49
CGTATGC42950.042.96449346-47
Produced by FastQC (version 0.11.2)