##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2578654 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.688955555883034 33.0 33.0 33.0 33.0 33.0 2 32.72284067579442 33.0 33.0 33.0 33.0 33.0 3 32.75063114322433 33.0 33.0 33.0 33.0 33.0 4 36.69356028377595 37.0 37.0 37.0 37.0 37.0 5 36.685773275515054 37.0 37.0 37.0 37.0 37.0 6 36.57006213319042 37.0 37.0 37.0 37.0 37.0 7 36.52127737959416 37.0 37.0 37.0 37.0 37.0 8 36.45284439091092 37.0 37.0 37.0 37.0 37.0 9 36.45460150915943 37.0 37.0 37.0 37.0 37.0 10-11 36.40522710685497 37.0 37.0 37.0 37.0 37.0 12-13 36.39780695665257 37.0 37.0 37.0 37.0 37.0 14-15 38.25399200513136 40.0 37.0 40.0 37.0 40.0 16-17 38.2298193165892 40.0 37.0 40.0 37.0 40.0 18-19 38.24329805394597 40.0 37.0 40.0 37.0 40.0 20-21 38.1848516706778 40.0 37.0 40.0 37.0 40.0 22-23 38.092538587961 40.0 37.0 40.0 37.0 40.0 24-25 38.057430543221386 40.0 37.0 40.0 35.0 40.0 26-27 37.96261693115866 40.0 37.0 40.0 35.0 40.0 28-29 37.85326084848917 40.0 37.0 40.0 33.0 40.0 30-31 37.81871821500674 40.0 37.0 40.0 33.0 40.0 32-33 37.677390995457316 40.0 37.0 40.0 33.0 40.0 34-35 37.601325342601214 40.0 37.0 40.0 33.0 40.0 36-37 37.52102162601109 40.0 37.0 40.0 33.0 40.0 38-39 37.42058182292001 40.0 37.0 40.0 33.0 40.0 40-41 37.35713554435764 40.0 37.0 40.0 33.0 40.0 42-43 37.220168933094556 40.0 37.0 40.0 33.0 40.0 44-45 37.08745105004394 40.0 37.0 40.0 33.0 40.0 46-47 37.020981100993 38.5 37.0 40.0 33.0 40.0 48-49 36.82598363332188 37.0 37.0 40.0 33.0 40.0 50-51 36.664725666956485 37.0 37.0 40.0 33.0 40.0 52-53 36.50998641151546 37.0 37.0 40.0 33.0 40.0 54-55 36.343073556979725 37.0 37.0 40.0 33.0 40.0 56-57 36.159529351359275 37.0 37.0 40.0 33.0 40.0 58-59 35.91706506572809 37.0 37.0 40.0 33.0 40.0 60-61 35.64521064090025 37.0 35.0 40.0 27.0 40.0 62-63 35.36508193809638 37.0 33.0 40.0 27.0 40.0 64-65 35.077096617072314 37.0 33.0 37.0 27.0 40.0 66-67 34.77040521915697 37.0 33.0 37.0 27.0 40.0 68-69 34.47193128663248 37.0 33.0 37.0 27.0 40.0 70-71 34.10191033771882 37.0 33.0 37.0 27.0 40.0 72-73 33.785652127039924 37.0 33.0 37.0 27.0 37.0 74-75 33.433257428100084 37.0 33.0 37.0 27.0 37.0 76-77 30.695082395699462 33.0 30.0 35.0 24.5 37.0 78-79 33.18630378484279 35.0 33.0 37.0 27.0 37.0 80-81 33.61263221044778 37.0 33.0 37.0 27.0 37.0 82-83 33.57503468863989 37.0 33.0 37.0 27.0 37.0 84-85 33.410962851161884 37.0 33.0 37.0 27.0 37.0 86-87 33.25303084477406 37.0 33.0 37.0 27.0 37.0 88-89 33.117795369212 37.0 33.0 37.0 27.0 37.0 90-91 32.97774168228851 37.0 33.0 37.0 27.0 37.0 92-93 32.86026353283535 33.0 33.0 37.0 27.0 37.0 94-95 32.67946494566545 33.0 33.0 37.0 27.0 37.0 96-97 32.51103579619445 33.0 33.0 37.0 27.0 37.0 98-99 32.37837511352822 33.0 33.0 37.0 24.5 37.0 100-101 30.750680393724792 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15477445440355808 2306 2 0.1796230620938033 2306 3 0.15873288632057125 2306 4 0.17143163152410779 2306 5 0.14946500324316503 2306 6 0.20105919712334241 2306 7 0.26026177070190215 2306 8 0.24300524960501235 2306 9 0.18749218087448583 2306 10-11 0.20216008555804166 2306 12-13 0.2422987794986895 2306 14-15 0.47265324229110917 2306 16-17 0.3332783116633564 2306 18-19 0.4360011530243426 2306 20-21 0.39052802030108325 2306 22-23 0.46410424205718925 2306 24-25 0.4414081101084406 2306 26-27 0.5152178762723878 2306 28-29 0.5003213744921027 2306 30-31 0.4234427874537019 2306 32-33 0.5731180304516741 2306 34-35 0.5300532969418512 2306 36-37 0.43218553855346187 2306 38-39 0.41205483678922405 2306 40-41 0.5344707924145169 2306 42-43 0.5115667202268313 2306 44-45 0.5996815643317461 2306 46-47 0.5468229658199064 2306 48-49 0.5180926458703325 2306 50-51 0.514882799452522 2306 52-53 0.6812330662904742 2306 54-55 0.665893942745484 2306 56-57 0.6903330174614979 2306 58-59 0.5095052689724326 2306 60-61 0.5013105681794698 2306 62-63 0.5243494362549441 2306 64-65 0.5469368368620593 2306 66-67 0.5527174808176767 2306 68-69 0.42372310647584754 2306 70-71 0.5031197142360213 2306 72-73 0.4588768995223518 2306 74-75 0.2366307182443066 2306 76-77 0.043253831914892515 2306 78-79 0.37602458298123764 2306 80-81 0.5772264679679395 2306 82-83 0.6251055450592631 2306 84-85 0.6043783234416509 2306 86-87 0.5010348663924802 2306 88-89 0.45910075476852086 2306 90-91 0.4731997964136099 2306 92-93 0.4752150533492241 2306 94-95 0.5067836258676337 2306 96-97 0.611041546996475 2306 98-99 0.5243136565757354 2306 100-101 0.3559278537202424 2307 1 0.07888021644144061 2307 2 0.06802724371920021 2307 3 0.09874208637076975 2307 4 0.1023181072526711 2307 5 0.11517233492226353 2307 6 0.11156178074646306 2307 7 0.16342037225474115 2307 8 0.1645540696565746 2307 9 0.13021050419251168 2307 10-11 0.20216230898585508 2307 12-13 0.11896980902482568 2307 14-15 0.1900817541459361 2307 16-17 0.2755776423621725 2307 18-19 0.18996379548430298 2307 20-21 0.24632069208065133 2307 22-23 0.17524403538710231 2307 24-25 0.2463449351128162 2307 26-27 0.21099044826451063 2307 28-29 0.26997709398840186 2307 30-31 0.3321334009320225 2307 32-33 0.34744974216499 2307 34-35 0.25185220321299795 2307 36-37 0.23854226926473387 2307 38-39 0.23431611722671875 2307 40-41 0.2955094938931424 2307 42-43 0.089955026577627 2307 44-45 0.30915835718040796 2307 46-47 0.1214534321171925 2307 48-49 0.22912596171270394 2307 50-51 0.22965493268822001 2307 52-53 0.25302347412642945 2307 54-55 0.14785428469880202 2307 56-57 0.12164169742722919 2307 58-59 0.1849926443493075 2307 60-61 0.23392150981597837 2307 62-63 0.006010974214788689 2307 64-65 0.026305773697934853 2307 66-67 0.13180616692995528 2307 68-69 0.16722279173728793 2307 70-71 0.22024645752694738 2307 72-73 0.16265381164556914 2307 74-75 0.13986182824864102 2307 76-77 -0.3282651447944538 2307 78-79 -0.09679574132300672 2307 80-81 0.21639402647620187 2307 82-83 0.10421051160408723 2307 84-85 0.3588241908799006 2307 86-87 0.11203641192185643 2307 88-89 -0.01692439368233778 2307 90-91 -0.039672862440426115 2307 92-93 -0.08587757860627221 2307 94-95 0.08388740856067756 2307 96-97 0.10724887324052901 2307 98-99 -0.045201566987458364 2307 100-101 0.06454566229002623 2201 1 0.10900754743057206 2201 2 0.12880541835698267 2201 3 0.10733418437197884 2201 4 0.10060874714830703 2201 5 0.0370206613832309 2201 6 0.059876004628243606 2201 7 0.10458097480587014 2201 8 0.12280849861198107 2201 9 0.19733280490783045 2201 10-11 0.12002040077206999 2201 12-13 0.05648655613395448 2201 14-15 0.11876301381249732 2201 16-17 0.22445109855758716 2201 18-19 0.13476870419861342 2201 20-21 0.12422764655081409 2201 22-23 0.15146004171342042 2201 24-25 0.19461622780146115 2201 26-27 0.2551759984587392 2201 28-29 0.20702993631508093 2201 30-31 0.1679965196116413 2201 32-33 0.2179131489005215 2201 34-35 0.27255168643862504 2201 36-37 0.12020575947382639 2201 38-39 0.24937899395920482 2201 40-41 0.2800315029467839 2201 42-43 0.25796957495612816 2201 44-45 0.22979627189179297 2201 46-47 0.17534289680239823 2201 48-49 0.24838313713767235 2201 50-51 0.36319550762366504 2201 52-53 0.19893287511121116 2201 54-55 0.27385181519741764 2201 56-57 0.2034972850065202 2201 58-59 0.36257972199256017 2201 60-61 0.33215416443081125 2201 62-63 0.24130940685439128 2201 64-65 0.15971451268597292 2201 66-67 0.2298107566238059 2201 68-69 0.08015641650693794 2201 70-71 0.09216670782144121 2201 72-73 0.14924429290987717 2201 74-75 0.1971950281685011 2201 76-77 0.061993373103572225 2201 78-79 0.1593610537608896 2201 80-81 0.2745287213480978 2201 82-83 0.2958252720919674 2201 84-85 0.36382471709688247 2201 86-87 0.33173775054124377 2201 88-89 0.22774723677677144 2201 90-91 0.2715331029446375 2201 92-93 0.2476263526966065 2201 94-95 0.259644220140963 2201 96-97 0.32080947371834156 2201 98-99 0.1646587252196028 2201 100-101 0.17761605840408734 1101 1 0.09726941225288499 1101 2 0.09374720384820989 1101 3 0.05960951405100445 1101 4 0.14696156358444057 1101 5 0.08501502290777552 1101 6 0.10354791809554342 1101 7 0.06796413396534007 1101 8 0.053959248811224825 1101 9 0.13868276741992958 1101 10-11 0.08858659795574653 1101 12-13 0.03799639175573333 1101 14-15 0.12851982171950027 1101 16-17 0.04930893086014976 1101 18-19 0.06038072774672543 1101 20-21 0.11056401059207133 1101 22-23 0.12388473188153881 1101 24-25 0.17786078949065853 1101 26-27 0.09220123470022656 1101 28-29 0.09490602367451118 1101 30-31 0.15065962378202613 1101 32-33 0.12807837273292932 1101 34-35 0.0638722682081152 1101 36-37 0.021975869683934945 1101 38-39 0.061858123164483914 1101 40-41 0.15157074804538695 1101 42-43 0.16885920906479868 1101 44-45 0.007687401616749412 1101 46-47 0.10759078119394161 1101 48-49 0.06458899239230931 1101 50-51 0.13182210824813723 1101 52-53 0.25988082942954804 1101 54-55 0.16125070921718532 1101 56-57 0.01737689969701961 1101 58-59 0.11858255764984449 1101 60-61 0.12113342016929352 1101 62-63 0.06040058854724606 1101 64-65 -0.001195902422978179 1101 66-67 0.10890843635586123 1101 68-69 0.07670861766127501 1101 70-71 0.08833397301512491 1101 72-73 -0.04455900779027644 1101 74-75 -0.08147982747794202 1101 76-77 -0.09576417943028304 1101 78-79 0.04472535957593493 1101 80-81 0.08082527207149326 1101 82-83 0.28701618041974797 1101 84-85 0.28193875050124007 1101 86-87 0.13868164225099378 1101 88-89 0.2060393525781521 1101 90-91 0.16679768253524685 1101 92-93 0.08628164033746089 1101 94-95 0.06997115607329363 1101 96-97 0.1993437574429251 1101 98-99 0.19517529060154715 1101 100-101 0.17510971583756074 2304 1 0.07079827374294467 2304 2 0.07177546086073505 2304 3 0.05246639944019904 2304 4 0.11103372250663313 2304 5 0.11455918447864377 2304 6 0.08960898453543109 2304 7 0.03567650831382707 2304 8 0.04205297706352695 2304 9 0.03767923362459413 2304 10-11 0.04464044194275374 2304 12-13 0.10394490083510277 2304 14-15 0.06018830369416861 2304 16-17 0.10521437794753297 2304 18-19 0.03589571610059039 2304 20-21 0.02117113719059205 2304 22-23 0.08734468922577321 2304 24-25 0.022051864278239464 2304 26-27 0.03767629263528249 2304 28-29 0.05090417829679694 2304 30-31 -1.4126366232858345E-4 2304 32-33 0.18845279598087927 2304 34-35 0.19078604180910474 2304 36-37 0.14372655259384004 2304 38-39 0.26315858426097805 2304 40-41 0.2413494419428872 2304 42-43 0.05341153236978613 2304 44-45 -0.0034077507194041345 2304 46-47 0.08298609462875817 2304 48-49 0.032253782848677304 2304 50-51 0.14887812049674665 2304 52-53 0.16015128521075894 2304 54-55 0.16560496910075528 2304 56-57 0.09608475767616653 2304 58-59 0.04420111487191036 2304 60-61 0.10900687028372857 2304 62-63 0.14983904331231912 2304 64-65 0.09802814638074153 2304 66-67 0.16901223217728756 2304 68-69 0.11214688819372753 2304 70-71 0.0937736033598 2304 72-73 0.13957816274371737 2304 74-75 0.04093435988358607 2304 76-77 -0.3400307018640376 2304 78-79 0.06419499084940838 2304 80-81 0.225607512351651 2304 82-83 0.2574161387671481 2304 84-85 0.2008337705179386 2304 86-87 0.10927021971384221 2304 88-89 0.2565325372107097 2304 90-91 0.14000461372512518 2304 92-93 0.03396768048224885 2304 94-95 0.09105339496121445 2304 96-97 0.09963862500045195 2304 98-99 0.04496505371386661 2304 100-101 0.08922517705516242 2202 1 0.05400858021609167 2202 2 0.04507257010950383 2202 3 0.011011243181414443 2202 4 0.03627648007567785 2202 5 -0.0071359598751072895 2202 6 0.03408394562671191 2202 7 0.03448317781536048 2202 8 0.012288465425903894 2202 9 -0.00893082053833183 2202 10-11 0.09463536958119079 2202 12-13 0.0853481564075409 2202 14-15 0.047783640232104574 2202 16-17 0.0083997856646576 2202 18-19 -0.014186166307581516 2202 20-21 0.03334233063066705 2202 22-23 -0.023783594863232338 2202 24-25 -0.1138070986200006 2202 26-27 0.06523989421701515 2202 28-29 -0.008428047953856321 2202 30-31 -0.006524927900976252 2202 32-33 -0.055253675822271475 2202 34-35 -0.11846462016058723 2202 36-37 -0.1257533483059987 2202 38-39 -0.03366196407013433 2202 40-41 -0.12192938206101189 2202 42-43 -0.22298047740591187 2202 44-45 -0.11497796651923409 2202 46-47 -0.17906801032565056 2202 48-49 -0.2624524567216113 2202 50-51 -0.1381458772523274 2202 52-53 -0.20862654429214444 2202 54-55 -0.13979352587477223 2202 56-57 -0.0881602045144092 2202 58-59 -0.18986742810902513 2202 60-61 -0.18381093084222755 2202 62-63 -0.1531237460553072 2202 64-65 -0.19729366933381698 2202 66-67 -0.19564514305410796 2202 68-69 -0.13946380752573617 2202 70-71 -0.18852585190037985 2202 72-73 -0.25186402099453176 2202 74-75 -0.337217176122941 2202 76-77 -0.522166569045222 2202 78-79 -0.32615769557579455 2202 80-81 -0.07133127325783306 2202 82-83 -0.08093821393764955 2202 84-85 0.03186881152277721 2202 86-87 -0.1607555344508782 2202 88-89 -0.158073283739526 2202 90-91 -0.13770559147752692 2202 92-93 -0.09911093900120704 2202 94-95 -0.1191064745642123 2202 96-97 -0.10948026161087654 2202 98-99 -0.17297046331530197 2202 100-101 -0.21809038781327317 1102 1 0.0755897836154773 1102 2 0.11492163995857396 1102 3 0.06104474776037705 1102 4 0.0174554158000646 1102 5 -0.017713834089349234 1102 6 4.3577561490337757E-4 1102 7 0.04005663793427772 1102 8 -0.012223059085620491 1102 9 0.0017215082958159655 1102 10-11 0.05582855804710363 1102 12-13 -0.046476407235203965 1102 14-15 -0.10170528198409556 1102 16-17 -0.05672141582017076 1102 18-19 0.032661744176692764 1102 20-21 -0.0074215737695979556 1102 22-23 -0.058519334144428115 1102 24-25 -0.0684083941303868 1102 26-27 -0.05676995506556182 1102 28-29 -0.11121530983203343 1102 30-31 -0.033554614021575446 1102 32-33 0.013037165195846967 1102 34-35 -0.0915093886599081 1102 36-37 -0.07729402711940025 1102 38-39 -0.14324586910859693 1102 40-41 -0.03294547489529265 1102 42-43 -0.10679670213122705 1102 44-45 -0.22929771720261272 1102 46-47 -0.10156075100021411 1102 48-49 -0.08321625475813477 1102 50-51 -0.1041653205444959 1102 52-53 -0.06829554032478313 1102 54-55 -0.00973991854843348 1102 56-57 -0.2767856658671448 1102 58-59 -0.3319401383635494 1102 60-61 -0.2730102245869759 1102 62-63 -0.25332370989162456 1102 64-65 -0.1271175309758661 1102 66-67 -0.23248954353486795 1102 68-69 -0.15785409864329125 1102 70-71 -0.10679329289509099 1102 72-73 -0.19940808217496198 1102 74-75 -0.43698902589478905 1102 76-77 -0.4116679358193167 1102 78-79 -0.25361230484858055 1102 80-81 -0.052411822520198825 1102 82-83 -0.05108457044764947 1102 84-85 -0.11512588092646325 1102 86-87 -0.14788286248730032 1102 88-89 -0.13985849279746532 1102 90-91 -0.014919286873038118 1102 92-93 -0.0824969223871932 1102 94-95 -0.052621158078899555 1102 96-97 -0.048201450332065576 1102 98-99 -0.13287665049421804 1102 100-101 -0.18797615315358485 2305 1 -0.041861620715458514 2305 2 0.009040302482141271 2305 3 0.03176104345575226 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1213 70-71 -0.09047933209813408 1213 72-73 0.005922044043430219 1213 74-75 0.1697233174587751 1213 76-77 0.3333445631834664 1213 78-79 -0.1217916362478988 1213 80-81 -0.22221133444323726 1213 82-83 -0.1704918550180139 1213 84-85 -0.2026599573571417 1213 86-87 -0.23651779914444404 1213 88-89 -0.3262556923799522 1213 90-91 -0.27289977247323094 1213 92-93 -0.17102671320705554 1213 94-95 -0.04028771826453692 1213 96-97 -0.116912919806893 1213 98-99 -0.17998198559400436 1213 100-101 0.1393765517147365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 12.0 10 19.0 11 17.0 12 36.0 13 95.0 14 269.0 15 630.0 16 1233.0 17 2023.0 18 2902.0 19 3784.0 20 4809.0 21 6161.0 22 7847.0 23 9751.0 24 12146.0 25 14949.0 26 18947.0 27 24507.0 28 33002.0 29 44230.0 30 50193.0 31 59767.0 32 79966.0 33 112529.0 34 171551.0 35 294419.0 36 571356.0 37 848142.0 38 202862.0 39 495.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.452606165684415 26.079367631671545 21.794677597640426 14.673348605003609 2 32.57459124023618 22.638399723266478 31.24808524137011 13.538923795127225 3 37.733787755273 18.892858444825197 26.263227579916183 17.11012621998562 4 31.79338731114263 18.656046402765675 31.62887095316713 17.921695332924564 5 31.28298717082633 17.212972349140287 33.87996218182044 17.624078298212943 6 27.10375257789529 17.886889827018283 37.17699233786308 17.832365257223344 7 33.85816786587111 18.824937351036628 27.695766861316017 19.621127921776242 8 31.073265354716067 26.697145099730324 23.378592087189674 18.85099745836394 9 29.101849259342277 25.493610232315 25.610299016463628 19.7942414918791 10-11 24.055495619032254 28.240236960833055 30.587333546881435 17.116933873253256 12-13 23.762831806242875 27.091490860919137 30.025466804154576 19.12021052868341 14-15 22.755728376121805 27.813754772838855 30.72800771255081 18.70250913848853 16-17 22.499164777276203 27.947802990979593 31.33298056524461 18.22005166649959 18-19 21.554093724865766 27.878963211039558 31.707006833797784 18.859936230296892 20-21 22.099649662188103 28.11937933511049 31.219950408236237 18.561020594465173 22-23 21.42810468351929 28.649503800149574 30.936864795647796 18.985526720683335 24-25 21.69693279726524 27.414376307005305 31.410029075263147 19.478661820466307 26-27 21.53495583354727 27.71713459812755 30.96830749685689 19.779602071468293 28-29 21.440662454133047 28.680272731432755 30.468414917239766 19.41064989719443 30-31 20.561676750738954 28.969667896507246 30.728317951923756 19.740337400830047 32-33 20.332991552957473 29.58671461933241 30.941394231253984 19.138899596456135 34-35 21.12565315082985 28.899922207477236 31.050365811000624 18.924058830692292 36-37 20.467267031559878 29.172661396216785 31.756509403743195 18.603562168480146 38-39 21.090890405845744 29.192196236176027 30.42663199706045 19.290281360917774 40-41 20.995565903762195 28.543748017376508 30.891329352445112 19.569356726416185 42-43 20.893632879789223 29.089323344659658 30.836126134021857 19.180917641529263 44-45 20.500676904844358 28.499206367365236 31.384845320165716 19.61527140762469 46-47 21.160593928244012 28.389244202731064 30.71615991139474 19.73400195763018 48-49 20.756004803416143 29.924243232180174 30.07562491237915 19.244127052024535 50-51 21.469809952108466 29.108663696495768 29.62354761105614 19.797978740339627 52-53 20.796363311311776 28.87341026239142 29.52215137654616 20.808075049750652 54-55 21.79173750636006 28.878143576029085 29.741075749866592 19.589043167744258 56-57 21.246252491471456 29.145722212106705 29.534739823385 20.073285473036844 58-59 21.01002650167257 29.27962648290209 30.01456457618169 19.69578243924365 60-61 21.099617217068616 29.523944147547393 29.93854647969036 19.437892155693625 62-63 20.639048889933374 29.30146213356012 29.710128909587226 20.34936006691928 64-65 21.104264643776656 29.016206724446903 29.701592440300455 20.177936191475986 66-67 21.198610014950873 29.18701000021331 29.434562304024325 20.179817680811496 68-69 21.318216308445948 29.677425860343465 29.25263766784961 19.751720163360982 70-71 20.561964630644717 30.76901596850938 28.99091051355705 19.678108887288857 72-73 20.39070130733415 30.520771544137336 29.000890637025563 20.087636511502946 74-75 20.870724501658472 29.779604041610636 29.083210600259267 20.266460856471628 76-77 20.818166446515736 29.281657717136945 29.273377818558917 20.626798017788403 78-79 20.660092129869142 29.340993758468958 29.525672339399055 20.473241772262842 80-81 20.11095915565248 29.757971989910054 29.492363414115335 20.638705440322127 82-83 19.538623638533902 29.51326544778788 29.65549081032197 21.292620103356246 84-85 19.725374935576582 29.37130110682246 29.944781034055506 20.958542923545444 86-87 20.011599240988485 29.34885250504437 30.547401960042926 20.092146293924223 88-89 20.204031176708412 29.88217112285985 29.92595580229414 19.9878418981376 90-91 20.46884119938646 29.618089692202982 29.416149405561793 20.496919702848764 92-93 19.89807731463975 30.09790860699846 29.59009860670758 20.413915471654207 94-95 19.594670981406068 30.443384849455015 29.62543172764552 20.3365124414934 96-97 19.851737022868715 30.958816766189308 29.20603795040435 19.983408260537626 98-99 20.392861872972023 30.254219227846352 28.81177104116463 20.541147858016995 100-101 20.390770798784395 30.092772142217783 28.291295451407578 21.22516160759024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 366.0 1 235.0 2 104.5 3 151.0 4 178.5 5 142.0 6 120.5 7 133.0 8 148.0 9 155.0 10 160.0 11 153.5 12 147.0 13 145.0 14 141.5 15 144.0 16 157.5 17 180.5 18 203.5 19 219.0 20 299.0 21 453.0 22 623.0 23 975.5 24 1514.5 25 2428.5 26 4184.5 27 6821.0 28 9781.5 29 12734.5 30 16159.5 31 19393.5 32 24624.5 33 35593.5 34 52499.0 35 76483.5 36 110989.0 37 150456.0 38 183023.5 39 199316.0 40 199267.0 41 185051.5 42 167022.5 43 158146.0 44 145355.5 45 130294.0 46 122231.0 47 108852.0 48 90517.0 49 75121.0 50 61933.5 51 50876.5 52 41215.5 53 32880.5 54 27133.0 55 21499.0 56 15055.0 57 9913.0 58 6755.0 59 4824.0 60 3581.5 61 2646.0 62 1891.0 63 1333.0 64 990.0 65 750.5 66 515.0 67 371.5 68 305.5 69 225.0 70 157.0 71 128.0 72 87.0 73 46.5 74 31.5 75 25.5 76 19.5 77 17.0 78 13.5 79 6.0 80 1.0 81 2.0 82 2.0 83 0.5 84 1.5 85 3.0 86 2.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15325825023442463 2 0.0 3 0.00542918902652314 4 0.007251845342570193 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.1633976485406726E-4 14-15 0.0 16-17 1.9389960809011213E-5 18-19 0.0 20-21 0.0 22-23 2.1328956889912336E-4 24-25 5.816988242703363E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010276679228775941 40-41 0.0 42-43 0.0 44-45 1.9389960809011213E-4 46-47 0.0010082779620685831 48-49 0.002811544317306626 50-51 5.623088634613252E-4 52-53 0.001725706512001998 54-55 0.0024043551403173905 56-57 0.004168841573937411 58-59 0.005332239222478084 60-61 0.0014930269822938634 62-63 9.694980404505607E-4 64-65 0.00127973741339474 66-67 0.008105003618166687 68-69 0.007465134911469317 70-71 0.00913267154104428 72-73 0.006301737262928645 74-75 0.00878365224648208 76-77 0.004653590594162691 78-79 0.004653590594162691 80-81 0.0018420462768560652 82-83 0.0 84-85 4.2657913779824673E-4 86-87 0.0014348570998668298 88-89 0.004905660084679837 90-91 0.006553806753445789 92-93 0.008880602050527136 94-95 0.007969273892503608 96-97 0.010179729424730886 98-99 0.007387575068233272 100-101 0.007213065420952171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2578654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.53613913962407 #Duplication Level Percentage of deduplicated Percentage of total 1 86.80907051219768 40.39758983937068 2 5.877097030447138 5.46994810291919 3 2.074165612374822 2.895709786082949 4 1.1208205520920522 2.0863464465082404 5 0.7109005777257997 1.6541284099743476 6 0.5573691701079124 1.5562685551367164 7 0.344304850600446 1.1215832903792884 8 0.29274148260218635 1.0898446693052146 9 0.2149863514160143 0.9004171286354183 >10 1.651237232716747 15.269942870104847 >50 0.18309985678485927 5.920803962128427 >100 0.14824041709686597 14.908350889197578 >500 0.013592109909063378 4.068206658708822 >1k 0.0022065503765874533 1.6096080899965342 >5k 8.384677649325682E-5 0.1993093205428411 >10k+ 8.384677522664604E-5 0.8519419810090457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 21834 0.8467208086079016 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5108 0.19808783962485854 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4839 0.1876560407096105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.041389810342916E-4 0.0 0.0 2 5.42918902652314E-4 0.0 0.0 3 5.816988242703364E-4 0.0 0.0 4 6.204787458883588E-4 0.0 0.0 5 7.368185107424261E-4 0.0 0.0 6 8.143783539784709E-4 0.0 0.0 7 9.307181188325382E-4 0.0 0.0 8 0.001085837805304628 0.0 0.0 9 0.0011633976485406728 0.0 0.0 10-11 0.0023074053362723344 0.0 0.0 12-13 0.0033544632199589398 0.0 0.0 14-15 0.004750540398207747 0.0 0.0 16-17 0.0069803858912440364 0.0 0.0 18-19 0.009462300874797472 0.0 0.0 20-21 0.012099335544822997 0.0 0.0 22-23 0.014581250528376432 0.0 0.0 24-25 0.017664254297009213 0.0 0.0 26-27 0.020921767712923096 0.0 0.0 28-29 0.02367514214780269 0.0 0.0 30-31 0.035309118633209416 0.0 0.0 32-33 0.039167720834202654 0.0 0.0 34-35 0.04285181338791478 0.0 0.0 36-37 0.04665224570648098 0.0 0.0 38-39 0.05120888649659861 0.0 0.0 40-41 0.05657990564069472 0.0 0.0 42-43 0.06197031474559984 0.0 0.0 44-45 0.06734133388969595 0.0 0.0 46-47 0.07375941091747865 0.0 0.0 48-49 0.08095308637762182 0.0 0.0 50-51 0.08808859195533794 0.0 0.0 52-53 0.09596091604379649 0.0 0.0 54-55 0.10476395825108759 0.0 0.0 56-57 0.11401296955698592 0.0 0.0 58-59 0.12442537851142495 0.0 0.0 60-61 0.134876567387482 0.0 0.0 62-63 0.14668505352016983 0.0 0.0 64-65 0.16056826545942185 0.0 0.0 66-67 0.17538219551750642 0.0 0.0 68-69 0.1928137702848075 0.0 0.0 70-71 0.21160264230873937 0.0 0.0 72-73 0.23223356060952732 0.0 0.0 74-75 0.25538517381548664 0.0 0.0 76-77 0.28355878687098 0.0 0.0 78-79 0.31206202926022647 0.0 0.0 80-81 0.3470996884421097 0.0 0.0 82-83 0.3854530309223339 0.0 0.0 84-85 0.4299917709006327 0.0 0.0 86-87 0.48236405504577196 0.0 0.0 88-89 0.5366753352718123 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 4310 0.0 51.030495 3 CGGAAGA 4315 0.0 50.969387 4 GAAGAGC 4445 0.0 49.58174 6 CGCGTTG 40 5.3905405E-4 47.498024 5 ATCGGAA 4715 0.0 46.747925 2 GATCGGA 4710 0.0 46.578083 1 ACTGGCG 200 0.0 42.753197 2 CGACGCC 45 9.539561E-4 42.300846 1 CACGCGG 45 9.539561E-4 42.300846 1 TGCCGTC 2615 0.0 41.685646 50-51 TATGCCG 2615 0.0 41.59483 48-49 ATGCCGT 2640 0.0 41.560772 48-49 GCCGTCT 2650 0.0 41.493553 50-51 TCGTATG 2670 0.0 41.449585 44-45 CGTATGC 2640 0.0 41.290897 46-47 TCTCGTA 2735 0.0 40.203995 42-43 CTCGTAT 2715 0.0 39.88784 44-45 GTATGCC 2810 0.0 39.384483 46-47 CGTCTTC 2820 0.0 39.161366 52-53 CCGTCTT 2785 0.0 39.141857 52-53 >>END_MODULE