FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578654
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT218340.8467208086079016TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC51080.19808783962485854No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG48390.1876560407096105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG43100.051.0304953
CGGAAGA43150.050.9693874
GAAGAGC44450.049.581746
CGCGTTG405.3905405E-447.4980245
ATCGGAA47150.046.7479252
GATCGGA47100.046.5780831
ACTGGCG2000.042.7531972
CGACGCC459.539561E-442.3008461
CACGCGG459.539561E-442.3008461
TGCCGTC26150.041.68564650-51
TATGCCG26150.041.5948348-49
ATGCCGT26400.041.56077248-49
GCCGTCT26500.041.49355350-51
TCGTATG26700.041.44958544-45
CGTATGC26400.041.29089746-47
TCTCGTA27350.040.20399542-43
CTCGTAT27150.039.8878444-45
GTATGCC28100.039.38448346-47
CGTCTTC28200.039.16136652-53
CCGTCTT27850.039.14185752-53
Produced by FastQC (version 0.11.2)