##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4682624 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67685105615997 33.0 33.0 33.0 33.0 33.0 2 32.7158338999672 33.0 33.0 33.0 33.0 33.0 3 32.748874562638385 33.0 33.0 33.0 33.0 33.0 4 36.68452111465708 37.0 37.0 37.0 37.0 37.0 5 36.68351377347402 37.0 37.0 37.0 37.0 37.0 6 36.55494462079381 37.0 37.0 37.0 37.0 37.0 7 36.4927730691168 37.0 37.0 37.0 37.0 37.0 8 36.42995828834431 37.0 37.0 37.0 37.0 37.0 9 36.445816063813794 37.0 37.0 37.0 37.0 37.0 10-11 36.38722530359047 37.0 37.0 37.0 37.0 37.0 12-13 36.37431608858623 37.0 37.0 37.0 37.0 37.0 14-15 38.2175975478706 40.0 37.0 40.0 37.0 40.0 16-17 38.19615113235656 40.0 37.0 40.0 37.0 40.0 18-19 38.213445922628 40.0 37.0 40.0 37.0 40.0 20-21 38.16933988293743 40.0 37.0 40.0 37.0 40.0 22-23 38.04963232153595 40.0 37.0 40.0 37.0 40.0 24-25 38.03331091712681 40.0 37.0 40.0 35.0 40.0 26-27 37.94430622659432 40.0 37.0 40.0 33.0 40.0 28-29 37.81052343728645 40.0 37.0 40.0 33.0 40.0 30-31 37.760799180117814 40.0 37.0 40.0 33.0 40.0 32-33 37.60920063195337 40.0 37.0 40.0 33.0 40.0 34-35 37.524287984685515 40.0 37.0 40.0 33.0 40.0 36-37 37.449409455040595 40.0 37.0 40.0 33.0 40.0 38-39 37.32004886576415 40.0 37.0 40.0 33.0 40.0 40-41 37.20314464710384 40.0 37.0 40.0 33.0 40.0 42-43 37.0687390232485 40.0 37.0 40.0 33.0 40.0 44-45 36.93087582944948 37.0 37.0 40.0 33.0 40.0 46-47 36.90254951070169 37.0 37.0 40.0 33.0 40.0 48-49 36.70300989359812 37.0 37.0 40.0 33.0 40.0 50-51 36.549727460500776 37.0 37.0 40.0 33.0 40.0 52-53 36.394242736551135 37.0 37.0 40.0 33.0 40.0 54-55 36.207818201931225 37.0 37.0 40.0 33.0 40.0 56-57 36.032533468414286 37.0 37.0 40.0 33.0 40.0 58-59 35.81284467853921 37.0 37.0 40.0 27.0 40.0 60-61 35.54357161711041 37.0 33.0 40.0 27.0 40.0 62-63 35.28678599861958 37.0 33.0 40.0 27.0 40.0 64-65 34.98200773754203 37.0 33.0 37.0 27.0 40.0 66-67 34.59937013947736 37.0 33.0 37.0 27.0 40.0 68-69 34.320445545061915 37.0 33.0 37.0 27.0 40.0 70-71 33.9038820968756 37.0 33.0 37.0 27.0 40.0 72-73 33.440574878529645 37.0 33.0 37.0 27.0 37.0 74-75 33.0174525009909 37.0 33.0 37.0 24.5 37.0 76-77 30.318198087226307 33.0 30.0 35.0 22.0 37.0 78-79 32.72473937689637 35.0 33.0 37.0 27.0 37.0 80-81 33.11640791573272 37.0 33.0 37.0 27.0 37.0 82-83 33.06420812347949 37.0 33.0 37.0 27.0 37.0 84-85 32.895971254578626 37.0 33.0 37.0 27.0 37.0 86-87 32.73655753697072 37.0 33.0 37.0 27.0 37.0 88-89 32.59917110577317 35.0 33.0 37.0 24.5 37.0 90-91 32.442966059201 33.0 33.0 37.0 22.0 37.0 92-93 32.31256812419703 33.0 33.0 37.0 22.0 37.0 94-95 32.128125170844385 33.0 33.0 37.0 22.0 37.0 96-97 31.96192006874778 33.0 33.0 37.0 22.0 37.0 98-99 31.827901834526966 33.0 33.0 37.0 22.0 37.0 100-101 30.246584180151984 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13690360940140778 2306 2 0.13101751306030707 2306 3 0.08529048736648548 2306 4 0.14867189268742464 2306 5 0.14765267422982475 2306 6 0.19159658264366186 2306 7 0.13723671659303704 2306 8 0.21719714395914735 2306 9 0.14619914653054167 2306 10-11 0.19707840377915176 2306 12-13 0.20002171471551833 2306 14-15 0.3093042075271555 2306 16-17 0.239299751345321 2306 18-19 0.36329011174697 2306 20-21 0.3897445558988579 2306 22-23 0.36820610480679505 2306 24-25 0.3506032223808191 2306 26-27 0.3920787316104466 2306 28-29 0.4106024118368694 2306 30-31 0.4741496672531653 2306 32-33 0.4680732008644952 2306 34-35 0.4793179052875942 2306 36-37 0.48268716042766613 2306 38-39 0.4688058481173343 2306 40-41 0.4841792285863349 2306 42-43 0.5251462273214784 2306 44-45 0.4967497795741096 2306 46-47 0.5327067083267139 2306 48-49 0.5433991565527663 2306 50-51 0.47231197286680526 2306 52-53 0.5393683681665848 2306 54-55 0.4445016195942628 2306 56-57 0.36589021765495744 2306 58-59 0.48400057361237003 2306 60-61 0.5238118844662125 2306 62-63 0.4947267118064431 2306 64-65 0.5803778783652049 2306 66-67 0.495345230700579 2306 68-69 0.3931819912195351 2306 70-71 0.49455476212558835 2306 72-73 0.3559361569796593 2306 74-75 0.3615866411147124 2306 76-77 0.07393192089212164 2306 78-79 0.30498506811068893 2306 80-81 0.4441758544359047 2306 82-83 0.515964171121837 2306 84-85 0.5297142017832925 2306 86-87 0.4742858822232847 2306 88-89 0.5164517422439587 2306 90-91 0.5785533913772696 2306 92-93 0.3253529215735753 2306 94-95 0.44915751823544525 2306 96-97 0.49999115718615883 2306 98-99 0.43994210345479345 2306 100-101 0.22316161997709472 2307 1 0.093488505694026 2307 2 0.14153628081906788 2307 3 0.14789930098712745 2307 4 0.14690626215253388 2307 5 0.15649790263479701 2307 6 0.11850409483455593 2307 7 0.16823387801780854 2307 8 0.15630909291943595 2307 9 0.1927163805354155 2307 10-11 0.20153022336899795 2307 12-13 0.16196067686909998 2307 14-15 0.37108290511761055 2307 16-17 0.38536089847641364 2307 18-19 0.38030472291963946 2307 20-21 0.2914376322436283 2307 22-23 0.28586740333484073 2307 24-25 0.22907813353992168 2307 26-27 0.31996674402368086 2307 28-29 0.38981382869383907 2307 30-31 0.4260899648466392 2307 32-33 0.4038566188934496 2307 34-35 0.39605093177593886 2307 36-37 0.42737309888154584 2307 38-39 0.4292284265196784 2307 40-41 0.38396447477833107 2307 42-43 0.31078151044415847 2307 44-45 0.2917144175026678 2307 46-47 0.25336182991534173 2307 48-49 0.21000770685386527 2307 50-51 0.22581444072203993 2307 52-53 0.3305510261959981 2307 54-55 0.2138902283626649 2307 56-57 0.25289539831383223 2307 58-59 0.23173627286471543 2307 60-61 0.29975898691493796 2307 62-63 0.27735802064116655 2307 64-65 0.2361285015704837 2307 66-67 0.3003330280302663 2307 68-69 0.26959268208710085 2307 70-71 0.30042072305820255 2307 72-73 0.22224319698538864 2307 74-75 0.14170004516277146 2307 76-77 -0.18909650819719914 2307 78-79 0.12432248501665555 2307 80-81 0.27510133312162566 2307 82-83 0.44215261841271314 2307 84-85 0.39115124344713337 2307 86-87 0.2044772693661301 2307 88-89 0.29036706828913594 2307 90-91 0.35813032307812165 2307 92-93 0.32855891941803606 2307 94-95 0.331803088249238 2307 96-97 0.39833668376871856 2307 98-99 0.2270222877878929 2307 100-101 0.1851594050782026 2201 1 0.12819684567421774 2201 2 0.1311257417255618 2201 3 0.07055644935166328 2201 4 0.12289521091854283 2201 5 0.05591991669955121 2201 6 0.03167711008244822 2201 7 0.040771112891214045 2201 8 0.13792263456532794 2201 9 0.06837078457803614 2201 10-11 0.12125974190376354 2201 12-13 0.11806622192816008 2201 14-15 0.3013123837712257 2201 16-17 0.23250209479471806 2201 18-19 0.20747735264971112 2201 20-21 0.21920522855661062 2201 22-23 0.23163547282111807 2201 24-25 0.19701280280883537 2201 26-27 0.23945656020479333 2201 28-29 0.2761442307623412 2201 30-31 0.25607670851716335 2201 32-33 0.22127482721598568 2201 34-35 0.27625121617841586 2201 36-37 0.24672725682420804 2201 38-39 0.30011620178272125 2201 40-41 0.30675227546561956 2201 42-43 0.3102356371465831 2201 44-45 0.45115148254883053 2201 46-47 0.1462289504793972 2201 48-49 0.2978000959510254 2201 50-51 0.3221450318105923 2201 52-53 0.24890537191242146 2201 54-55 0.27700381653657047 2201 56-57 0.25247072633632683 2201 58-59 0.2436034607282167 2201 60-61 0.29981174635846486 2201 62-63 0.24058920629125424 2201 64-65 0.12848817599071793 2201 66-67 0.2626205674129096 2201 68-69 0.12822676501996 2201 70-71 0.12494972039613828 2201 72-73 0.19002161313858323 2201 74-75 0.2910751677142329 2201 76-77 0.13040221892929793 2201 78-79 0.18251287972375962 2201 80-81 0.4191434937330101 2201 82-83 0.3080122525363507 2201 84-85 0.29340222302798225 2201 86-87 0.2580124740395391 2201 88-89 0.32480267933488705 2201 90-91 0.31671322519647305 2201 92-93 0.19487645981431712 2201 94-95 0.2977988998971952 2201 96-97 0.4538508868601667 2201 98-99 0.2706142067859467 2201 100-101 0.2729341903846567 1101 1 0.11186073740925906 1101 2 0.1182678750938635 1101 3 0.06979324873268666 1101 4 0.07653529208920418 1101 5 0.0850809787381337 1101 6 0.09985204893737176 1101 7 0.10831747011226867 1101 8 -0.00989440663347807 1101 9 0.06072313129179463 1101 10-11 0.018592802222237026 1101 12-13 0.06060468815660158 1101 14-15 0.1671284149229777 1101 16-17 0.18748566804120514 1101 18-19 0.15161519493668862 1101 20-21 0.12336433470813546 1101 22-23 0.1071871004158993 1101 24-25 0.12083396944554892 1101 26-27 0.1457192566095742 1101 28-29 0.08126031872924244 1101 30-31 0.14865075840119601 1101 32-33 0.09153719665040683 1101 34-35 0.09132438793659503 1101 36-37 0.013310885968600417 1101 38-39 0.10274939229583424 1101 40-41 -0.01019571520439655 1101 42-43 0.08137675291406055 1101 44-45 -0.06667797920723473 1101 46-47 0.02674057276495745 1101 48-49 0.004890227003549796 1101 50-51 -0.0039276440964926 1101 52-53 0.11962433980836096 1101 54-55 -0.0028689186136645617 1101 56-57 -0.04919693041386353 1101 58-59 -0.07489480649073954 1101 60-61 4.037461599040171E-4 1101 62-63 -0.06563148575283861 1101 64-65 -0.04683646079431014 1101 66-67 0.09439849842431869 1101 68-69 -0.010983428901525372 1101 70-71 -0.18426212995935032 1101 72-73 -0.2514307287731299 1101 74-75 -0.18207778697016153 1101 76-77 -0.2210833649690862 1101 78-79 -0.13695726102996986 1101 80-81 0.07861924046370206 1101 82-83 0.14583724677660115 1101 84-85 0.21322865895933063 1101 86-87 0.20176898579455838 1101 88-89 0.010750210843546881 1101 90-91 -0.1955168691339395 1101 92-93 -0.050835426421578234 1101 94-95 -0.01887432236333808 1101 96-97 0.06978669923313419 1101 98-99 0.06515676931633507 1101 100-101 0.07661236414188366 2304 1 0.08572005783295822 2304 2 0.07801725318667252 2304 3 0.056870864721489056 2304 4 0.11547363779808961 2304 5 0.1099002277925365 2304 6 0.10895801891776102 2304 7 0.0852146176093953 2304 8 0.11860913687217334 2304 9 0.052298493360936504 2304 10-11 0.06073371370806768 2304 12-13 0.005951811500395365 2304 14-15 0.10165414976087561 2304 16-17 0.04045275644833879 2304 18-19 0.10371683985127333 2304 20-21 0.07632597746636804 2304 22-23 0.08797037563883947 2304 24-25 0.13338771588768594 2304 26-27 0.1678845733655976 2304 28-29 0.11636272900786793 2304 30-31 0.06563982156649217 2304 32-33 0.021433856073421964 2304 34-35 0.10684918183521575 2304 36-37 0.02778269792846544 2304 38-39 0.1168288202147707 2304 40-41 0.10526272158453054 2304 42-43 0.03445830166286612 2304 44-45 -0.014262161036825205 2304 46-47 0.0910956631869908 2304 48-49 0.07443460614715036 2304 50-51 0.04602519157651841 2304 52-53 0.0528555594005482 2304 54-55 0.0811283770270208 2304 56-57 0.09656470383163906 2304 58-59 0.02106597732591098 2304 60-61 0.02365518037639447 2304 62-63 0.13809728149778522 2304 64-65 0.02834432144810961 2304 66-67 0.08188199521318751 2304 68-69 0.033410776302105205 2304 70-71 0.04717681391024797 2304 72-73 0.07851566131716226 2304 74-75 0.007949031285846786 2304 76-77 -0.4006458133390929 2304 78-79 0.1334957668838328 2304 80-81 0.1865078731880203 2304 82-83 0.3588275683875537 2304 84-85 0.35103307347148416 2304 86-87 0.28806288214538966 2304 88-89 0.3166072331408216 2304 90-91 0.09877202222740067 2304 92-93 0.10373270623568231 2304 94-95 0.12395190369399955 2304 96-97 0.1479495219445539 2304 98-99 0.19940132558435764 2304 100-101 0.27737078811476223 2202 1 0.05560892546669294 2202 2 0.05302427062629533 2202 3 0.03126921818444117 2202 4 0.04646895367449844 2202 5 0.05602697594017059 2202 6 0.04398541261431177 2202 7 0.04240157247372167 2202 8 0.04270397045404195 2202 9 0.04842529703726939 2202 10-11 0.026093729878027716 2202 12-13 0.06721045003103399 2202 14-15 0.03608145172400867 2202 16-17 -0.05461411537836369 2202 18-19 -0.10160969528826058 2202 20-21 0.003213210612777573 2202 22-23 -0.0018006198325153377 2202 24-25 0.011291346381945289 2202 26-27 0.06658352895401976 2202 28-29 0.004007100160599464 2202 30-31 0.06244647007341797 2202 32-33 -0.0150689768620893 2202 34-35 -0.07202653410327287 2202 36-37 -0.1622890618121673 2202 38-39 -0.061500076838079565 2202 40-41 -0.1184295497364829 2202 42-43 -0.09527493012698329 2202 44-45 -0.11498348580726514 2202 46-47 -0.17093317231228866 2202 48-49 -0.1631329341062937 2202 50-51 -0.058633534578419244 2202 52-53 -0.06460971642022884 2202 54-55 -0.10075997918738011 2202 56-57 -0.14682689484244804 2202 58-59 -0.19596028164162504 2202 60-61 -0.2760660516611182 2202 62-63 -0.38643043995693205 2202 64-65 -0.3515422042626497 2202 66-67 -0.15159207329979552 2202 68-69 -0.14488223532754319 2202 70-71 -0.08572575320937403 2202 72-73 -0.23381100237131136 2202 74-75 -0.30780895908603156 2202 76-77 -0.7422064988520134 2202 78-79 -0.2539220124623185 2202 80-81 -0.04902700425042639 2202 82-83 0.0608300166479907 2202 84-85 -0.03441361050718683 2202 86-87 -0.023414678289967128 2202 88-89 0.015552409931714806 2202 90-91 0.06235012237397086 2202 92-93 -0.047622811831828926 2202 94-95 -0.11184836623101546 2202 96-97 -0.1022455226186132 2202 98-99 -0.02718423401029213 2202 100-101 -0.05384132569353639 1102 1 0.06372623277396627 1102 2 0.04470274594544321 1102 3 0.042213087857639664 1102 4 -0.02048962061918047 1102 5 -0.03339817578667237 1102 6 0.012629016574649654 1102 7 0.015616494473590592 1102 8 -0.023790297158221563 1102 9 -0.007870221621530504 1102 10-11 0.013524847418516117 1102 12-13 -0.03557039673054874 1102 14-15 -0.0986448703320022 1102 16-17 -0.0745574871732444 1102 18-19 -0.13781070917601568 1102 20-21 -0.1726032831700408 1102 22-23 -0.14828162119332688 1102 24-25 -0.20181923932560863 1102 26-27 -0.10507462643346344 1102 28-29 -0.09859613202473838 1102 30-31 -0.08815160201761074 1102 32-33 -0.06366386437407101 1102 34-35 -0.1989587213307331 1102 36-37 -0.08996399429317847 1102 38-39 -0.06486184627920721 1102 40-41 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0.045653525001796424 1213 68-69 0.01009806743020647 1213 70-71 -0.07001552426182656 1213 72-73 0.07778886416539166 1213 74-75 0.1256584467774715 1213 76-77 0.25980051800281245 1213 78-79 -0.16354377896283978 1213 80-81 -0.23920548598723457 1213 82-83 -0.23266480196107153 1213 84-85 -0.3516573412570665 1213 86-87 -0.2216145453488707 1213 88-89 -0.472859287510623 1213 90-91 -0.32366879335492627 1213 92-93 -0.21180628634923693 1213 94-95 -0.033714551960748906 1213 96-97 -0.09595270480467022 1213 98-99 -0.21702120732876296 1213 100-101 0.0799946227389512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 25.0 10 50.0 11 48.0 12 76.0 13 191.0 14 427.0 15 1086.0 16 2086.0 17 3518.0 18 5321.0 19 7523.0 20 9948.0 21 12589.0 22 16264.0 23 20434.0 24 25958.0 25 32335.0 26 40877.0 27 53398.0 28 73936.0 29 105196.0 30 115996.0 31 115481.0 32 147551.0 33 206028.0 34 314374.0 35 536030.0 36 1012925.0 37 1459554.0 38 363165.0 39 229.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.14847319982512 24.87423180874337 23.925836978757154 15.051458012674354 2 28.755906944482412 23.88562481207118 33.72784148374928 13.630626759697126 3 37.0580172502534 19.05212023152398 27.499665767263814 16.39019675095881 4 27.924627591970353 19.174136113108588 34.46878743488506 18.432448860036004 5 33.65546753273379 18.020131447666948 32.09542342071454 16.22897759888473 6 26.954587855014623 19.390901340786705 37.4450735314217 16.209437272776974 7 31.982943751195908 21.547939787606264 29.11739229970205 17.351724161495778 8 27.762382800754448 28.662476423475386 26.468236612634282 17.106904163135884 9 31.86264795123418 23.802978842631823 25.736958594155755 18.59741461197824 10-11 24.982445739824506 28.8885462509909 28.839962380067245 17.28904562911735 12-13 23.945774818656602 27.104240648107048 28.8412377553898 20.108746777846555 14-15 22.90624871866714 27.603380070661238 29.562644790613124 19.927726420058498 16-17 23.126659326053083 27.13472189951617 30.451419973074927 19.28719880135582 18-19 21.161372341661426 27.36302338176202 31.686069605417817 19.789534671158734 20-21 22.45607377402072 27.541107293688327 31.243187580296862 18.759631351994095 22-23 21.30049513984329 29.981325611439676 29.833790574137343 18.884388674579693 24-25 21.36136932640431 27.141280950732 31.171489657453776 20.325860065409916 26-27 21.24721096547577 27.34326950017768 29.615724004318945 21.79379553002761 28-29 22.016747447584944 28.90501137823579 29.35579495599049 19.72244621818878 30-31 20.410906363611513 28.167967361889403 30.530723799305687 20.890402475193394 32-33 20.320519008145858 29.275466063472106 29.77423555681601 20.62977937156603 34-35 20.58081110078452 29.4213030984337 31.115588183035836 18.882297617745948 36-37 20.45211189281907 28.480911557280702 31.087804615531805 19.979171934368424 38-39 22.191103347587223 29.41436256902309 29.2773548095212 19.117179273868484 40-41 20.81678990241369 30.48886692589454 29.037191540469614 19.657151631222153 42-43 20.87102231569308 29.414255767706308 30.557589078260396 19.157132838340214 44-45 20.474670416706044 28.27617246123562 30.814571123949374 20.434585998108965 46-47 21.899915473984226 28.101312481020084 29.481384415692023 20.517387629303663 48-49 20.80777319392782 29.656577866215766 30.156812516384136 19.37883642347228 50-51 22.004868019440043 28.45268202885041 29.791563558939714 19.750886392769832 52-53 20.5856330205911 28.168359723117092 28.88537181715033 22.360635439141475 54-55 22.059588579453575 28.381884238104877 29.86022661360109 19.69830056884046 56-57 20.824146488354312 28.391344646571643 29.620773019079877 21.163735845994168 58-59 20.760672479962068 28.334048782988102 30.001761928254282 20.903516808795548 60-61 21.7679553055541 28.648051953737607 29.920446132935457 19.663546607772837 62-63 20.56168981040278 28.59270345254848 29.84440537058114 21.0012013664676 64-65 21.975049761770297 28.44165067381404 29.709333173591673 19.873966390823995 66-67 21.05048910806189 30.49432991314534 28.403410968770874 20.051770010021897 68-69 21.205042626112373 30.51262925720522 28.293497882709985 19.988830233972426 70-71 20.954431575495324 31.189021059649196 28.180766714283166 19.67578065057231 72-73 20.61512958130572 31.578238657010647 28.041794572168712 19.76483718951492 74-75 21.090902450682435 30.94316012580395 27.960997444367035 20.00493997914658 76-77 21.16901838590976 30.205237069103596 28.370565031960176 20.255179513026466 78-79 20.948984557222655 30.00152378617404 28.854220647441476 20.19527100916183 80-81 20.555985284890617 30.187554430998258 28.7329040216259 20.523556262485226 82-83 19.845774505918047 30.18909910340869 28.98156033881858 20.983566051854687 84-85 19.932746814886208 29.996763552583516 29.047494625585955 21.02299500694432 86-87 19.964013259419318 30.021304594625413 29.499314263699457 20.515367882255813 88-89 20.08449753704576 30.280999960490217 29.26591897078092 20.368583531683107 90-91 20.28902385306406 29.974974972302682 28.946743729727565 20.789257444905694 92-93 19.927705118039814 30.65298723626918 28.783518475507037 20.63578917018397 94-95 19.676614081064002 31.01473792930454 28.718934330235417 20.589713659396033 96-97 20.04698494576623 31.108822508008092 28.45609206734189 20.388100478883793 98-99 20.34553896237933 30.516461052784226 28.18717891573965 20.950821069096794 100-101 20.357689180105197 30.544825675112165 27.754730470374728 21.342754674407914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 42.0 1 32.0 2 73.5 3 199.0 4 220.5 5 136.5 6 102.0 7 102.5 8 104.0 9 99.5 10 100.5 11 107.5 12 114.0 13 119.5 14 135.0 15 156.0 16 184.5 17 214.0 18 277.0 19 406.5 20 566.5 21 818.0 22 1281.0 23 2004.5 24 3049.5 25 4662.0 26 7307.5 27 11115.0 28 15320.5 29 19503.0 30 24739.0 31 31368.0 32 44239.5 33 67050.5 34 100199.5 35 140730.0 36 195057.5 37 261287.5 38 319780.5 39 342529.5 40 336754.0 41 314206.0 42 288535.0 43 286240.0 44 277494.5 45 254941.0 46 239056.0 47 210851.5 48 168797.5 49 138454.5 50 116395.0 51 98033.0 52 82921.0 53 67690.5 54 52332.5 55 38742.5 56 28924.0 57 21507.5 58 16484.0 59 12879.5 60 9769.5 61 7248.5 62 5393.0 63 3968.0 64 2862.5 65 2005.5 66 1396.0 67 961.5 68 661.0 69 483.5 70 346.5 71 251.0 72 169.0 73 103.0 74 77.5 75 54.5 76 34.0 77 23.0 78 21.0 79 14.0 80 5.5 81 4.0 82 4.5 83 4.5 84 3.5 85 3.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1570914085777547 2 0.0 3 0.0055097312959571385 4 0.006983264084410791 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.3388869146871496E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.601666074406145E-4 24-25 1.06777738293743E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.076107754968154E-4 40-41 0.0 42-43 0.0 44-45 1.388110597818659E-4 46-47 8.969330016674412E-4 48-49 0.0025946990405379547 50-51 4.591442746630949E-4 52-53 0.0014308216931361562 54-55 0.0024772435284148376 56-57 0.004356531722384714 58-59 0.005424309105322145 60-61 0.001569632752918022 62-63 8.435441325205697E-4 64-65 0.0013560772763305362 66-67 0.008040363693518848 68-69 0.007292919525462647 70-71 0.009044074433480032 72-73 0.006267853237842714 74-75 0.008926618921356913 76-77 0.004997198152147172 78-79 0.004452631686849083 80-81 0.001921999289287374 82-83 0.0 84-85 4.3778872700434625E-4 86-87 0.0014094661454774075 88-89 0.005103975890440916 90-91 0.0066522530957001886 92-93 0.008787807861575047 94-95 0.008232563622447585 96-97 0.010378796162151819 98-99 0.007356986168438892 100-101 0.00739969726375639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4682624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.34597984416082 #Duplication Level Percentage of deduplicated Percentage of total 1 91.00002305676526 43.08485257463778 2 4.102281855185872 3.884531080613939 3 1.3574205556032055 1.928052187969169 4 0.7913555718643346 1.498700198202126 5 0.5197395523219849 1.2303789184224934 6 0.3308665352600332 0.9399120185717317 7 0.2382009213375965 0.7894499214357267 8 0.16290601344752556 0.6170355863343311 9 0.12714908161403218 0.5417998069771376 >10 1.0602779664263802 9.936201223885421 >50 0.13315756369427117 4.445340732350611 >100 0.14094231209600708 14.137810501291314 >500 0.027347266625725942 8.909701440549272 >1k 0.008150096025735331 5.352785176207645 >5k 1.3623889309477994E-4 0.45483647023422163 >10k+ 4.541283935781593E-5 2.2486121623171136 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 104581 2.2333845296995873 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8034 0.17157046989038624 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7240 0.15461416504933986 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5880 0.12557062023344176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015162438837711505 0.0 2.13555476587486E-5 2 0.0015803105267473963 0.0 2.13555476587486E-5 3 0.0018152215509936308 0.0 2.13555476587486E-5 4 0.0019433548369461224 0.0 2.13555476587486E-5 5 0.0020501325752398654 0.0 2.13555476587486E-5 6 0.0022636880518273517 0.0 2.13555476587486E-5 7 0.002669443457343575 0.0 2.13555476587486E-5 8 0.002989776672224804 0.0 2.13555476587486E-5 9 0.0033955320777410273 0.0 2.13555476587486E-5 10-11 0.005424309105322144 0.0 2.13555476587486E-5 12-13 0.007506475002050133 0.0 2.13555476587486E-5 14-15 0.010090496268758714 0.0 2.13555476587486E-5 16-17 0.012781295273761036 0.0 4.27110953174972E-5 18-19 0.015632260886203973 0.0 4.27110953174972E-5 20-21 0.01899575964245688 0.0 4.27110953174972E-5 22-23 0.023533813519940956 0.0 4.27110953174972E-5 24-25 0.0286271116365525 0.0 4.27110953174972E-5 26-27 0.03382718749145778 0.0 4.27110953174972E-5 28-29 0.040116396276959246 0.0 4.27110953174972E-5 30-31 0.06069246644616352 0.0 4.27110953174972E-5 32-33 0.06759030833993931 0.0 4.27110953174972E-5 34-35 0.07487255009157259 0.0 4.27110953174972E-5 36-37 0.08208004742640024 0.0 4.27110953174972E-5 38-39 0.08987482232184348 0.0 4.27110953174972E-5 40-41 0.09850246357597792 0.0 4.27110953174972E-5 42-43 0.10832601549900227 0.0 4.27110953174972E-5 44-45 0.11907853374518218 0.0 4.27110953174972E-5 46-47 0.1297563075745565 0.0 4.27110953174972E-5 48-49 0.14150185878686822 0.0 4.27110953174972E-5 50-51 0.15397349861957738 0.0 4.27110953174972E-5 52-53 0.16770511576415276 1.06777738293743E-5 4.27110953174972E-5 54-55 0.1821841770767843 2.13555476587486E-5 4.27110953174972E-5 56-57 0.19762423803405954 2.13555476587486E-5 4.27110953174972E-5 58-59 0.21473003170871718 2.13555476587486E-5 4.27110953174972E-5 60-61 0.23297834718311783 2.13555476587486E-5 4.27110953174972E-5 62-63 0.2526788398983134 2.13555476587486E-5 5.3388869146871496E-5 64-65 0.2737460876636689 2.13555476587486E-5 6.406664297624579E-5 66-67 0.2967673680397999 2.13555476587486E-5 6.406664297624579E-5 68-69 0.32407043572150995 2.13555476587486E-5 6.406664297624579E-5 70-71 0.3529858472514556 2.13555476587486E-5 7.474441680562009E-5 72-73 0.38353495817729544 2.13555476587486E-5 8.54221906349944E-5 74-75 0.41674283478664953 2.13555476587486E-5 8.54221906349944E-5 76-77 0.4540936876418008 2.13555476587486E-5 1.0677773829374299E-4 78-79 0.4948614281223519 2.13555476587486E-5 1.0677773829374299E-4 80-81 0.5396013004674303 2.13555476587486E-5 1.0677773829374299E-4 82-83 0.5880997492004483 2.13555476587486E-5 1.0677773829374299E-4 84-85 0.6442861950906158 2.13555476587486E-5 1.0677773829374299E-4 86-87 0.7044661283929694 2.13555476587486E-5 1.0677773829374299E-4 88-89 0.7723020255309843 2.13555476587486E-5 1.0677773829374299E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 16615 0.0 67.27242 3 CGGAAGA 16605 0.0 67.07978 4 GATCGGA 17005 0.0 65.9413 1 ATCGGAA 17135 0.0 65.31406 2 GAAGAGC 17500 0.0 63.7292 6 GGAAGAG 19930 0.0 56.268726 5 TTGTGTT 56080 0.0 46.14933 1 GCCGTCT 12710 0.0 43.986885 50-51 ATGCCGT 12795 0.0 43.675644 48-49 TCGTATG 12995 0.0 43.25885 44-45 TGCCGTC 12775 0.0 43.223938 50-51 TCTCGTA 12775 0.0 43.185833 42-43 TATGCCG 12810 0.0 43.105377 48-49 CGTCTTC 13025 0.0 43.050503 52-53 AATCTCG 12700 0.0 43.04793 40-41 CGTATGC 12915 0.0 42.86481 46-47 CTCGTAT 12790 0.0 42.708107 44-45 ATCTCGT 12810 0.0 42.456028 42-43 GTATGCC 13240 0.0 42.27899 46-47 CCGTCTT 13325 0.0 41.47526 52-53 >>END_MODULE