FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4682624
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1045812.2333845296995873TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC80340.17157046989038624No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA72400.15461416504933986No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG58800.12557062023344176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG166150.067.272423
CGGAAGA166050.067.079784
GATCGGA170050.065.94131
ATCGGAA171350.065.314062
GAAGAGC175000.063.72926
GGAAGAG199300.056.2687265
TTGTGTT560800.046.149331
GCCGTCT127100.043.98688550-51
ATGCCGT127950.043.67564448-49
TCGTATG129950.043.2588544-45
TGCCGTC127750.043.22393850-51
TCTCGTA127750.043.18583342-43
TATGCCG128100.043.10537748-49
CGTCTTC130250.043.05050352-53
AATCTCG127000.043.0479340-41
CGTATGC129150.042.8648146-47
CTCGTAT127900.042.70810744-45
ATCTCGT128100.042.45602842-43
GTATGCC132400.042.2789946-47
CCGTCTT133250.041.4752652-53
Produced by FastQC (version 0.11.2)