##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574902 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67580125379529 33.0 33.0 33.0 33.0 33.0 2 32.71649522972137 33.0 33.0 33.0 33.0 33.0 3 32.73962387694755 33.0 33.0 33.0 33.0 33.0 4 36.68774229077456 37.0 37.0 37.0 37.0 37.0 5 36.68268772947475 37.0 37.0 37.0 37.0 37.0 6 36.569959555742315 37.0 37.0 37.0 37.0 37.0 7 36.51779252181248 37.0 37.0 37.0 37.0 37.0 8 36.446019304812374 37.0 37.0 37.0 37.0 37.0 9 36.452801698860775 37.0 37.0 37.0 37.0 37.0 10-11 36.40781959857113 37.0 37.0 37.0 37.0 37.0 12-13 36.39910625724785 37.0 37.0 37.0 37.0 37.0 14-15 38.253642468723086 40.0 37.0 40.0 37.0 40.0 16-17 38.23479670294248 40.0 37.0 40.0 37.0 40.0 18-19 38.25854634467642 40.0 37.0 40.0 37.0 40.0 20-21 38.204960615976844 40.0 37.0 40.0 37.0 40.0 22-23 38.11528613516165 40.0 37.0 40.0 37.0 40.0 24-25 38.085839577583926 40.0 37.0 40.0 37.0 40.0 26-27 37.990404100816264 40.0 37.0 40.0 37.0 40.0 28-29 37.88236503758202 40.0 37.0 40.0 33.0 40.0 30-31 37.84680038308254 40.0 37.0 40.0 33.0 40.0 32-33 37.717151565379964 40.0 37.0 40.0 33.0 40.0 34-35 37.653962558575046 40.0 37.0 40.0 33.0 40.0 36-37 37.571132804277596 40.0 37.0 40.0 33.0 40.0 38-39 37.4666797027615 40.0 37.0 40.0 33.0 40.0 40-41 37.40243861708135 40.0 37.0 40.0 33.0 40.0 42-43 37.284595103036935 40.0 37.0 40.0 33.0 40.0 44-45 37.15086554750433 40.0 37.0 40.0 33.0 40.0 46-47 37.11653511473446 40.0 37.0 40.0 33.0 40.0 48-49 36.91805998830247 37.0 37.0 40.0 33.0 40.0 50-51 36.73995651096624 37.0 37.0 40.0 33.0 40.0 52-53 36.609845928116876 37.0 37.0 40.0 33.0 40.0 54-55 36.452583826491264 37.0 37.0 40.0 33.0 40.0 56-57 36.27673907589492 37.0 37.0 40.0 33.0 40.0 58-59 36.01839235046616 37.0 37.0 40.0 33.0 40.0 60-61 35.75381995120591 37.0 37.0 40.0 30.0 40.0 62-63 35.496048393298075 37.0 33.0 40.0 27.0 40.0 64-65 35.20733293927303 37.0 33.0 37.0 27.0 40.0 66-67 34.90902022678921 37.0 33.0 37.0 27.0 40.0 68-69 34.647427164218286 37.0 33.0 37.0 27.0 40.0 70-71 34.26009688912433 37.0 33.0 37.0 27.0 40.0 72-73 33.9531896747915 37.0 33.0 37.0 27.0 37.0 74-75 33.605721887667954 37.0 33.0 37.0 27.0 37.0 76-77 30.88922393939653 33.0 30.0 35.0 24.5 37.0 78-79 33.35435756389952 35.0 33.0 37.0 27.0 37.0 80-81 33.79463451424559 37.0 33.0 37.0 27.0 37.0 82-83 33.77460617918663 37.0 33.0 37.0 27.0 37.0 84-85 33.62403112817498 37.0 33.0 37.0 27.0 37.0 86-87 33.49332654213636 37.0 33.0 37.0 27.0 37.0 88-89 33.382939816738656 37.0 33.0 37.0 27.0 37.0 90-91 33.2524414521407 37.0 33.0 37.0 27.0 37.0 92-93 33.14087176910034 37.0 33.0 37.0 27.0 37.0 94-95 32.98105733732779 35.0 33.0 37.0 27.0 37.0 96-97 32.82876338594634 33.0 33.0 37.0 27.0 37.0 98-99 32.7058231730761 33.0 33.0 37.0 27.0 37.0 100-101 31.13968395690399 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17247768322945944 2306 2 0.15317242989726765 2306 3 0.12012166015755099 2306 4 0.18971241955036788 2306 5 0.17118226239496437 2306 6 0.17982204947836067 2306 7 0.22021804446298887 2306 8 0.1939794558254846 2306 9 0.19532260347329355 2306 10-11 0.2880054708261426 2306 12-13 0.18849886841715602 2306 14-15 0.38687956512169563 2306 16-17 0.4232781687380509 2306 18-19 0.4131533493462456 2306 20-21 0.3302308478209781 2306 22-23 0.25383878047389175 2306 24-25 0.36612630295113746 2306 26-27 0.3294302076512068 2306 28-29 0.4265961308244073 2306 30-31 0.4678842138615309 2306 32-33 0.3978916225954663 2306 34-35 0.5237798769415818 2306 36-37 0.4886906780161482 2306 38-39 0.39862999012726874 2306 40-41 0.5247952996604468 2306 42-43 0.6171437244501519 2306 44-45 0.49821387226833735 2306 46-47 0.5041827418002995 2306 48-49 0.3131974496610468 2306 50-51 0.3955672454168848 2306 52-53 0.5415959224195106 2306 54-55 0.4240159398526018 2306 56-57 0.49231227963825575 2306 58-59 0.46808174307762584 2306 60-61 0.33209712428617877 2306 62-63 0.3281773020211247 2306 64-65 0.37287287497353105 2306 66-67 0.3716867787411857 2306 68-69 0.5298634868684715 2306 70-71 0.5048013567731502 2306 72-73 0.503761037515126 2306 74-75 0.47710231548182236 2306 76-77 -0.07574691141958922 2306 78-79 0.20397290351697706 2306 80-81 0.43793866785329527 2306 82-83 0.5109934115068526 2306 84-85 0.5012654004151713 2306 86-87 0.3762072547311064 2306 88-89 0.4182919758857011 2306 90-91 0.38977353258223246 2306 92-93 0.3625700678009949 2306 94-95 0.4259670855822719 2306 96-97 0.6425889702519854 2306 98-99 0.4367290932931738 2306 100-101 0.15352227424116904 2307 1 0.0902214273276698 2307 2 0.13192265121454483 2307 3 0.10557595076189585 2307 4 0.13424994020665082 2307 5 0.1400617330040319 2307 6 0.11292484397788627 2307 7 0.18620674252720448 2307 8 0.0666050902683537 2307 9 0.15154752084817602 2307 10-11 0.18200334618024527 2307 12-13 0.1816312087736236 2307 14-15 0.30213312095266787 2307 16-17 0.3753393703433403 2307 18-19 0.3145836122711785 2307 20-21 0.2316072568743337 2307 22-23 0.39722877103101695 2307 24-25 0.3341324924577478 2307 26-27 0.32242606901805715 2307 28-29 0.31525306690797805 2307 30-31 0.4466189429691809 2307 32-33 0.5177714473121853 2307 34-35 0.4387687741913382 2307 36-37 0.4489988992153968 2307 38-39 0.39380158479204397 2307 40-41 0.3279278613341958 2307 42-43 0.31666770786610954 2307 44-45 0.4381977898792897 2307 46-47 0.3591776883890603 2307 48-49 0.247207829441507 2307 50-51 0.30904380791687913 2307 52-53 0.3313465347306561 2307 54-55 0.2819256350249404 2307 56-57 0.32998896460048144 2307 58-59 0.13818122519518994 2307 60-61 0.2208916459457697 2307 62-63 0.336820848409225 2307 64-65 0.2088092683871281 2307 66-67 0.43059756574543684 2307 68-69 0.37996953473201955 2307 70-71 0.42038172445491995 2307 72-73 0.3508710026945394 2307 74-75 0.39211777080523547 2307 76-77 -0.16054868490873275 2307 78-79 0.17467307761990725 2307 80-81 0.3657785374236511 2307 82-83 0.4577631013675756 2307 84-85 0.4724426976885425 2307 86-87 0.36691838403942256 2307 88-89 0.2888752206921126 2307 90-91 0.4553098212389912 2307 92-93 0.3558866547934443 2307 94-95 0.4268899786417606 2307 96-97 0.49485581249702904 2307 98-99 0.4254577730618294 2307 100-101 0.34861954318592936 2201 1 0.10499149206712133 2201 2 0.09620084308170362 2201 3 0.14122591688185082 2201 4 0.1396152323505646 2201 5 0.1355993643402087 2201 6 0.1780888152084259 2201 7 0.06396429206145626 2201 8 0.14280007602241085 2201 9 0.12720450789211668 2201 10-11 0.1597453843708152 2201 12-13 0.16323638792965056 2201 14-15 0.32478294828693066 2201 16-17 0.28411960725870955 2201 18-19 0.26700950478572594 2201 20-21 0.14646467497036042 2201 22-23 0.2717542463720619 2201 24-25 0.2192432352878413 2201 26-27 0.33382445747108846 2201 28-29 0.29708030319471845 2201 30-31 0.3454294678240686 2201 32-33 0.2711974133015218 2201 34-35 0.2687570998642528 2201 36-37 0.2904370456914691 2201 38-39 0.4118061728842335 2201 40-41 0.45738471938907566 2201 42-43 0.378390609956881 2201 44-45 0.21976541525584992 2201 46-47 0.29307732183151813 2201 48-49 0.3236705899708454 2201 50-51 0.330945825207948 2201 52-53 0.34073906152853795 2201 54-55 0.2990934269443457 2201 56-57 0.42581559676121117 2201 58-59 0.42157429080817366 2201 60-61 0.26434545071509774 2201 62-63 0.11670508480209918 2201 64-65 0.11018487965652213 2201 66-67 0.1922486258233178 2201 68-69 0.2691295546518475 2201 70-71 0.3457974692851522 2201 72-73 0.12314406765442243 2201 74-75 0.2712239908540539 2201 76-77 0.06200562520384878 2201 78-79 0.36085818509719303 2201 80-81 0.4494303975603131 2201 82-83 0.36643720803663626 2201 84-85 0.34913159728116483 2201 86-87 0.3566319140130503 2201 88-89 0.4141725894783974 2201 90-91 0.4191422660716668 2201 92-93 0.25470615989994627 2201 94-95 0.3565754249194484 2201 96-97 0.43584984471164034 2201 98-99 0.292979468533332 2201 100-101 0.5215073471878533 1101 1 0.08805423361705778 1101 2 0.11769822931587015 1101 3 0.0955637628707251 1101 4 0.1007013972635491 1101 5 0.06799972848023828 1101 6 0.12266664734657695 1101 7 0.10075946888159848 1101 8 0.15505322036036517 1101 9 0.1432998321554635 1101 10-11 0.13708492819048246 1101 12-13 0.143236332079951 1101 14-15 0.16290609128449063 1101 16-17 0.17951605633495404 1101 18-19 0.14550709207880175 1101 20-21 0.14885518163881528 1101 22-23 0.15088335411729759 1101 24-25 0.2381057724278861 1101 26-27 0.1652541538721337 1101 28-29 0.2077174365414578 1101 30-31 0.22059436405533006 1101 32-33 0.27399687937841577 1101 34-35 0.14388192393770538 1101 36-37 0.16804508693088138 1101 38-39 0.21770096880943157 1101 40-41 0.2873240666178134 1101 42-43 0.2668908780354329 1101 44-45 0.05008523854741043 1101 46-47 0.16095508918402146 1101 48-49 0.19759213231608896 1101 50-51 0.1673771412502134 1101 52-53 0.26111051786524797 1101 54-55 0.13290190157741222 1101 56-57 0.13234679998708998 1101 58-59 0.25244311662414276 1101 60-61 0.2747221848482013 1101 62-63 0.16694607624592805 1101 64-65 0.1855999825316701 1101 66-67 0.2558089323264525 1101 68-69 -0.03056983435246252 1101 70-71 0.12666727247081866 1101 72-73 -0.0324096262445579 1101 74-75 0.11016463379576891 1101 76-77 -0.06246868952036522 1101 78-79 0.04277195874954032 1101 80-81 0.2857614633959358 1101 82-83 0.2708877307898021 1101 84-85 0.31091141543455336 1101 86-87 0.20770101684350806 1101 88-89 0.23742351658336958 1101 90-91 0.049664823764409505 1101 92-93 -0.08887433626876629 1101 94-95 0.2017967851946736 1101 96-97 0.1669121897287269 1101 98-99 0.20364103612264017 1101 100-101 0.16423418073341622 2304 1 0.07615172053391461 2304 2 0.09256914695502871 2304 3 0.07775459487475445 2304 4 0.09973940134941017 2304 5 0.10048195779207703 2304 6 0.0598588086540488 2304 7 0.03667130108152605 2304 8 0.10879171139106347 2304 9 0.12668353909302965 2304 10-11 0.11573561523047005 2304 12-13 0.10271531509285126 2304 14-15 0.17394193350316556 2304 16-17 0.06772950748655404 2304 18-19 0.122345728633249 2304 20-21 0.11286166724035951 2304 22-23 0.14854395496245587 2304 24-25 0.0712808137062595 2304 26-27 0.16087269975409413 2304 28-29 0.14092868384787494 2304 30-31 0.04436633254383793 2304 32-33 0.15396252737885874 2304 34-35 0.1977501969626374 2304 36-37 0.196521040531195 2304 38-39 0.21308076479633797 2304 40-41 0.23411694714841502 2304 42-43 0.21942425054822934 2304 44-45 0.26065652419372753 2304 46-47 0.15034864695974193 2304 48-49 0.276465302774767 2304 50-51 0.09216655159919185 2304 52-53 0.20118691783166298 2304 54-55 0.22774332966908872 2304 56-57 0.2670166080076797 2304 58-59 0.25369983310470445 2304 60-61 0.33780725327234506 2304 62-63 0.23399719146156173 2304 64-65 0.04826632037064371 2304 66-67 0.11094459124118572 2304 68-69 0.01965247415787985 2304 70-71 0.162854060593844 2304 72-73 0.15648789145616604 2304 74-75 0.036846552470386484 2304 76-77 -0.2069071287781945 2304 78-79 0.16427816449772337 2304 80-81 0.33024415446581656 2304 82-83 0.2655119227132374 2304 84-85 0.23779447870939663 2304 86-87 0.16398529887973012 2304 88-89 0.26763106282913895 2304 90-91 0.26353138268753185 2304 92-93 0.18674886217525 2304 94-95 0.2930840756544768 2304 96-97 0.24564980621769195 2304 98-99 0.18316364127753815 2304 100-101 0.21146380740903936 2202 1 0.05202754421863176 2202 2 0.050716533826154375 2202 3 0.03881518700858777 2202 4 0.07376698587317065 2202 5 0.05098876755300097 2202 6 0.06897520374379695 2202 7 0.07003033411766069 2202 8 0.06441416189074545 2202 9 0.09980067268605097 2202 10-11 0.03633185361806568 2202 12-13 0.0368756083388746 2202 14-15 0.06535243514657907 2202 16-17 0.003984471781656396 2202 18-19 -0.07683324113882861 2202 20-21 0.026099219656657624 2202 22-23 -0.001282671091814791 2202 24-25 -0.08806156972591594 2202 26-27 0.10413235180178049 2202 28-29 -0.042403368462245794 2202 30-31 -0.036023750910196384 2202 32-33 0.07081434812224074 2202 34-35 -0.18478637955168864 2202 36-37 -0.04766819855686322 2202 38-39 0.01637879207341797 2202 40-41 -0.05668179327560097 2202 42-43 -0.11220670858686077 2202 44-45 -0.06124869540798272 2202 46-47 -0.051029192553670555 2202 48-49 -0.09209656509970898 2202 50-51 -0.03876128426924197 2202 52-53 0.11689869394789554 2202 54-55 0.009672503084239281 2202 56-57 -0.00468286870093948 2202 58-59 -0.17318715370683435 2202 60-61 -0.16398948083161002 2202 62-63 -0.22213238575323402 2202 64-65 -0.1719906977876633 2202 66-67 -0.05274065897830127 2202 68-69 -0.0930304876523067 2202 70-71 -0.06484524020519444 2202 72-73 -0.24339301300432936 2202 74-75 -0.1271372289833579 2202 76-77 -0.4518140710259715 2202 78-79 -0.16454240139229626 2202 80-81 0.09847159604323252 2202 82-83 0.04020483371566996 2202 84-85 0.11801134669480007 2202 86-87 0.08648999772569965 2202 88-89 -0.013269198024168816 2202 90-91 -0.021846846679544285 2202 92-93 0.0416739738223626 2202 94-95 8.92321534863072E-4 2202 96-97 0.09133015632529151 2202 98-99 0.10563010752350266 2202 100-101 -0.048843939268486736 1102 1 0.026637863802314143 1102 2 0.07188951297115409 1102 3 0.06034095498942804 1102 4 0.022047705197941525 1102 5 0.015030364563912713 1102 6 -0.038207183346465 1102 7 -0.044312427205753124 1102 8 -0.02751010804956877 1102 9 0.06569477553638592 1102 10-11 0.04102799054386708 1102 12-13 0.01881495198399108 1102 14-15 -0.10250999615891487 1102 16-17 -0.1336340614404392 1102 18-19 -0.04459096559252629 1102 20-21 -0.018078470749998132 1102 22-23 -0.031445569878954416 1102 24-25 0.055335794052219 1102 26-27 0.11826285670164083 1102 28-29 -0.03545738269261278 1102 30-31 -8.828121347335127E-4 1102 32-33 -0.0036616347208493494 1102 34-35 -0.040782295510688016 1102 36-37 -0.13057075048114797 1102 38-39 -0.057094311969045464 1102 40-41 -0.016009121909696944 1102 42-43 -0.11360027915610971 1102 44-45 -0.1457757888981206 1102 46-47 -0.2188687545415462 1102 48-49 -0.08904373898050721 1102 50-51 -0.27289890690254026 1102 52-53 -0.14462753324155386 1102 54-55 -0.08020610184000532 1102 56-57 -0.2601581113434861 1102 58-59 -0.18220632056392816 1102 60-61 -0.028239033741002117 1102 62-63 -0.12094658885683174 1102 64-65 -0.05109145799658421 1102 66-67 -0.09497591519301807 1102 68-69 -0.04222579745594146 1102 70-71 -0.14679730319986817 1102 72-73 -0.18197231268204206 1102 74-75 -0.21199934430958933 1102 76-77 -0.40352409463877237 1102 78-79 -0.142098307307009 1102 80-81 -0.006873592161234399 1102 82-83 -0.046400816518250565 1102 84-85 0.021929689928455787 1102 86-87 0.06331362991272016 1102 88-89 0.024370418704293684 1102 90-91 0.041396110026198585 1102 92-93 -0.09780070975192956 1102 94-95 -0.1318029305032411 1102 96-97 0.07110775722271967 1102 98-99 0.008248817504473038 1102 100-101 -0.22780855870299987 2305 1 -0.038610911764848765 2305 2 0.020283641738373603 2305 3 -0.012532338230769824 2305 4 -0.05674055139198941 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-0.3537741668473018 1213 88-89 -0.5308616352993241 1213 90-91 -0.5437840099381646 1213 92-93 -0.4235795491273322 1213 94-95 -0.1838424041729212 1213 96-97 -0.3240764144273598 1213 98-99 -0.4004179790635689 1213 100-101 -0.07282765840252381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 52.0 9 119.0 10 94.0 11 58.0 12 40.0 13 106.0 14 318.0 15 766.0 16 1527.0 17 2453.0 18 3178.0 19 3734.0 20 4437.0 21 5580.0 22 7026.0 23 8803.0 24 10839.0 25 13542.0 26 17527.0 27 22682.0 28 29904.0 29 37592.0 30 43510.0 31 54924.0 32 74492.0 33 106542.0 34 165337.0 35 291821.0 36 581453.0 37 881392.0 38 204942.0 39 108.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.97976774625179 24.33452163546158 25.091300889378992 13.594409728907635 2 32.450438890489814 21.007828647459203 33.719419224498644 12.82231323755234 3 38.55295812829883 17.48428014929489 27.96191504483896 16.000846677567317 4 31.669612628368775 17.136059153823478 34.761221744887095 16.43310647292065 5 30.50337449735951 16.03113438880392 37.3245273023983 16.14096381143826 6 25.349741465888798 16.872991671139328 41.62597256128583 16.151294301686043 7 34.33854958363464 17.861572984136874 30.158235148366813 17.641642283861678 8 30.756937545584258 27.73864791747414 24.451765542921635 17.052648994019968 9 29.22647153173208 26.481046657309676 25.39273339334856 18.89974841760968 10-11 24.638995969555346 27.45556918282715 30.173284264799204 17.7321505828183 12-13 24.319871777569656 26.01933550442046 29.63097020642157 20.02982251158831 14-15 23.205193828736007 26.890188442123236 30.77066622341355 19.13395150572721 16-17 22.287178308145318 27.858516557134987 31.547006449177484 18.307298685542207 18-19 20.93316561173979 28.156061861771825 31.874611150249603 19.036161376238788 20-21 21.548082218274715 28.13553680877952 31.48244088512883 18.833940087816934 22-23 20.83701228379471 28.295098463180945 31.813356381508928 19.054532871515416 24-25 21.563105059787745 27.043142055392046 31.90876696012137 19.48498592469884 26-27 21.38087197104977 27.390615254483468 31.623921997808075 19.604590776658686 28-29 21.090725006233246 28.647944659641418 30.93678905061241 19.324541283512925 30-31 20.094667680556384 28.95702438384063 30.89131159166446 20.056996343938525 32-33 19.62742659720642 29.811988961133277 31.354436013487113 19.20614842817319 34-35 20.20589521465283 29.45016936566906 31.639048787099473 18.70488663257864 36-37 20.45592414779281 30.22899512292118 31.32748353141207 17.98759719787394 38-39 21.021601998541676 30.26254647065734 30.348667072511216 18.367184458289763 40-41 21.1355616640944 29.57646543441265 29.856612018632166 19.431360882860783 42-43 20.446914096148124 29.729500384869016 30.90488492377574 18.91870059520712 44-45 19.86992113672786 29.27224926642768 31.83924265782024 19.018586939024225 46-47 20.555506146507195 29.022939736538873 31.17288709324756 19.248667023706375 48-49 20.529917965537564 30.652615267206702 29.798229593575503 19.019237173680228 50-51 20.791730447671288 29.72210673731301 29.741680377600378 19.74448243741532 52-53 19.949628329307227 29.520459364780997 29.79626076757571 20.733651538336066 54-55 21.155002684630226 29.33363885363862 29.986222458266866 19.52513600346429 56-57 21.00959607767898 29.445409782965836 29.32973098513983 20.215263154215354 58-59 20.541700448099583 29.5149941013977 30.311125783446048 19.632179667056672 60-61 20.442646284936433 29.83921424701585 30.348019597220038 19.37011987082768 62-63 20.350330957235087 29.888253023102635 29.84159064530981 19.91982537435247 64-65 20.949040046759734 29.6380781128331 29.588735720125133 19.824146120282034 66-67 21.08592532923394 29.988146141145588 29.12860543545259 19.79732309416788 68-69 21.88773507794778 30.070266315998595 28.664202047264066 19.377796558789562 70-71 20.99881246340533 31.09850533517565 28.754776120707316 19.147906080711707 72-73 20.282583762794005 31.20156551668692 28.85789289097428 19.6579578295448 74-75 21.057518256226672 30.1774460760886 28.73174595807345 20.03328970961127 76-77 21.301826970560413 29.258599224827424 29.184321077341902 20.255252727270257 78-79 21.103185729108066 29.296605787019818 29.659199943140763 19.941008540731353 80-81 19.71654276626934 29.846567791023208 30.030384288330524 20.406505154376926 82-83 19.095697622666805 29.71332501198104 30.134933290665046 21.056044074687115 84-85 19.061815686387973 29.250722895379745 30.638977182895495 21.048484235336794 86-87 19.184503868556842 29.336232388376544 31.59860606121804 19.880657681848582 88-89 19.522331912305503 29.95928389207697 31.043279628410897 19.47510456720663 90-91 19.984802298802805 29.7070976138677 30.044996924922074 20.26310316240742 92-93 19.11699934438047 30.67173036947734 30.1784038616301 20.03286642451209 94-95 18.92275616763332 30.885259629213756 30.37620825178633 19.81577595136659 96-97 19.524211523085924 31.551058860267965 29.445204100081895 19.479525516564212 98-99 20.023039474519766 30.723014986339248 28.87405054896442 20.37989499017657 100-101 19.549052651693607 30.711461821181622 28.622199833534843 21.117285693589928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1488.0 1 932.0 2 423.0 3 659.0 4 725.0 5 543.5 6 472.5 7 499.0 8 538.5 9 517.5 10 497.0 11 479.0 12 446.5 13 436.0 14 453.0 15 473.0 16 481.0 17 502.5 18 527.0 19 565.0 20 632.5 21 784.0 22 1026.5 23 1294.5 24 1688.0 25 2266.0 26 3395.5 27 5373.0 28 7478.5 29 9432.0 30 11933.0 31 15215.0 32 22494.5 33 34088.5 34 49936.5 35 76413.5 36 121785.0 37 181664.5 38 228975.0 39 235000.5 40 215287.0 41 189811.5 42 164353.0 43 152072.5 44 135622.5 45 116461.5 46 111465.5 47 99982.0 48 76154.0 49 57917.0 50 47042.5 51 39805.5 52 33941.5 53 27737.5 54 21945.5 55 16207.5 56 11306.5 57 8273.0 58 6641.0 59 5249.5 60 4067.0 61 3227.0 62 2399.0 63 1710.5 64 1241.5 65 868.0 66 604.0 67 449.5 68 324.5 69 242.5 70 198.0 71 154.0 72 105.5 73 75.0 74 52.0 75 36.5 76 25.0 77 13.0 78 9.0 79 10.0 80 6.5 81 3.5 82 2.0 83 3.0 84 4.0 85 2.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15561757301831294 2 0.0 3 0.005126408694389145 4 0.007417758035063083 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.825464425442212E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.136003622662144E-4 24-25 5.825464425442212E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.514925228222279E-4 40-41 0.0 42-43 0.0 44-45 9.709107375737018E-5 46-47 9.709107375737018E-4 48-49 0.002699131850454891 50-51 4.0778250978095473E-4 52-53 0.001631130039123819 54-55 0.00231076755542541 56-57 0.0038836429502948073 58-59 0.005417681915661256 60-61 0.0018253121866385594 62-63 8.738196638163316E-4 64-65 0.0014369478916090787 66-67 0.008272159484127939 68-69 0.007670194826832245 70-71 0.009495507013470803 72-73 0.00625266514997464 74-75 0.009029469859435427 76-77 0.004679789755105243 78-79 0.004757462614111139 80-81 0.0020000761194018256 82-83 0.0 84-85 5.24291798289799E-4 86-87 0.0015534571801179228 88-89 0.004757462614111139 90-91 0.006621611230252647 92-93 0.00924307022170164 94-95 0.008194486625122043 96-97 0.010505254180547454 98-99 0.007398339820311608 100-101 0.007340085176057186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2574902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.00158298506275 #Duplication Level Percentage of deduplicated Percentage of total 1 85.86039702165155 33.48691399570377 2 6.421728683025889 5.009151682771838 3 1.9871080927866764 2.3250108354332797 4 1.052464293492876 1.6419109392591134 5 0.7750409828804246 1.5113912605317736 6 0.5678651595035416 1.3288584085621962 7 0.419624269292604 1.1456207532953269 8 0.33804475940429735 1.054742458925782 9 0.28925136771280524 1.0153135099254487 >10 1.8551020143409043 14.166630801434039 >50 0.18949928569791843 5.221109006990037 >100 0.20671086862821467 18.427700977794327 >500 0.029806325536190583 7.622608635027234 >1k 0.006956490855021065 4.696303500239889 >5k 3.0028889340797814E-4 0.6266953248854565 >10k+ 1.0009629766725693E-4 0.7200379092203253 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 18444 0.7162991057523742 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5525 0.2145712730037881 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5315 0.206415622808169 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5213 0.2024543069988683 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4995 0.19398796536722562 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4951 0.19227916246909593 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4019 0.1560836101723483 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 3944 0.1531708779596272 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3713 0.14419966274444618 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3594 0.13957812763359537 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3372 0.1309564402839409 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3139 0.121907552209754 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2940 0.11417910273866733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.1069143602358457E-4 0.0 0.0 2 3.1069143602358457E-4 0.0 0.0 3 3.4952786552653264E-4 0.0 0.0 4 4.272007245324288E-4 0.0 0.0 5 5.04873583538325E-4 0.0 0.0 6 6.602193015501172E-4 0.0 0.0 7 7.378921605560134E-4 0.0 0.0 8 8.932378785678057E-4 0.0 0.0 9 9.709107375737018E-4 0.0 3.883642950294807E-5 10-11 0.001689384683378241 0.0 3.883642950294807E-5 12-13 0.002563204347194573 0.0 3.883642950294807E-5 14-15 0.0040389886683066 0.0 3.883642950294807E-5 16-17 0.0055341912041701 0.0 3.883642950294807E-5 18-19 0.00735950339080866 0.0 3.883642950294807E-5 20-21 0.00869936020866037 0.0 3.883642950294807E-5 22-23 0.010116889885517972 0.0 3.883642950294807E-5 24-25 0.011748019924641792 0.0 3.883642950294807E-5 26-27 0.013243222460505293 0.0 3.883642950294807E-5 28-29 0.015651081089688074 0.0 3.883642950294807E-5 30-31 0.02304942090999968 0.0 3.883642950294807E-5 32-33 0.02549611596868541 0.0 3.883642950294807E-5 34-35 0.02790397459786819 0.0 3.883642950294807E-5 36-37 0.030447760730311288 0.0 3.883642950294807E-5 38-39 0.03273911007098522 0.0 3.883642950294807E-5 40-41 0.03536056906243422 0.0 3.883642950294807E-5 42-43 0.03813737377189501 0.0 3.883642950294807E-5 44-45 0.041050105984616114 0.0 3.883642950294807E-5 46-47 0.0439045835530828 0.0 3.883642950294807E-5 48-49 0.04769113542962024 0.0 3.883642950294807E-5 50-51 0.05165245123892094 0.0 3.883642950294807E-5 52-53 0.056079804202257014 0.0 3.883642950294807E-5 54-55 0.06114795825239174 0.0 3.883642950294807E-5 56-57 0.0658860026517514 3.883642950294807E-5 3.883642950294807E-5 58-59 0.07140077564117003 3.883642950294807E-5 3.883642950294807E-5 60-61 0.07839133295170068 3.883642950294807E-5 3.883642950294807E-5 62-63 0.08639163742930799 3.883642950294807E-5 7.767285900589614E-5 64-65 0.09516867049697425 3.883642950294807E-5 7.767285900589614E-5 66-67 0.10505254180547455 3.883642950294807E-5 7.767285900589614E-5 68-69 0.11639277922033538 3.883642950294807E-5 7.767285900589614E-5 70-71 0.12940298310382298 3.883642950294807E-5 7.767285900589614E-5 72-73 0.14371420737565935 3.883642950294807E-5 7.767285900589614E-5 74-75 0.159307033821093 3.883642950294807E-5 7.767285900589614E-5 76-77 0.17895826714958474 3.883642950294807E-5 7.767285900589614E-5 78-79 0.20288150772340074 3.883642950294807E-5 7.767285900589614E-5 80-81 0.22921260692639953 3.883642950294807E-5 9.709107375737019E-5 82-83 0.26123324305158024 3.883642950294807E-5 1.1650928850884422E-4 84-85 0.29577824709445255 3.883642950294807E-5 1.1650928850884422E-4 86-87 0.3381487916821688 3.883642950294807E-5 1.1650928850884422E-4 88-89 0.3885973136064984 3.883642950294807E-5 1.1650928850884422E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAACG 20 0.0020748037 71.24624 9 CGGAAGA 3460 0.0 59.30323 4 TCGGAAG 3510 0.0 58.460724 3 GAAGAGC 3605 0.0 56.917942 6 GATCGGA 3770 0.0 55.021606 1 ATCGGAA 3835 0.0 53.754143 2 CGAGGCG 165 0.0 49.017715 1 CGCGGCG 40 5.347108E-4 47.576023 1 ACGGCGA 50 2.8013781E-5 47.57602 1 GGAAGAG 4505 0.0 45.65242 5 GCCGTCT 2190 0.0 44.570435 50-51 TATGCCG 2165 0.0 44.42649 48-49 ATGCCGT 2195 0.0 44.360275 48-49 TGCCGTC 2180 0.0 44.230175 50-51 TCGTATG 2270 0.0 44.045033 44-45 CGTATGC 2220 0.0 43.967278 46-47 AATCTCG 2235 0.0 43.778454 40-41 TCTCGTA 2225 0.0 43.548264 42-43 CTCGTAT 2215 0.0 43.101562 44-45 GTTGGCG 100 6.2755134E-10 42.818417 1 >>END_MODULE