FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574902
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT184440.7162991057523742TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.2145712730037881No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG53150.206415622808169No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52130.2024543069988683No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC49950.19398796536722562No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49510.19227916246909593No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT40190.1560836101723483No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT39440.1531708779596272No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA37130.14419966274444618No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA35940.13957812763359537No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT33720.1309564402839409No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG31390.121907552209754No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT29400.11417910273866733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG200.002074803771.246249
CGGAAGA34600.059.303234
TCGGAAG35100.058.4607243
GAAGAGC36050.056.9179426
GATCGGA37700.055.0216061
ATCGGAA38350.053.7541432
CGAGGCG1650.049.0177151
CGCGGCG405.347108E-447.5760231
ACGGCGA502.8013781E-547.576021
GGAAGAG45050.045.652425
GCCGTCT21900.044.57043550-51
TATGCCG21650.044.4264948-49
ATGCCGT21950.044.36027548-49
TGCCGTC21800.044.23017550-51
TCGTATG22700.044.04503344-45
CGTATGC22200.043.96727846-47
AATCTCG22350.043.77845440-41
TCTCGTA22250.043.54826442-43
CTCGTAT22150.043.10156244-45
GTTGGCG1006.2755134E-1042.8184171
Produced by FastQC (version 0.11.2)