##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5588832 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.665362995345 33.0 33.0 33.0 33.0 33.0 2 32.70408199781278 33.0 33.0 33.0 33.0 33.0 3 32.73659236849488 33.0 33.0 33.0 33.0 33.0 4 36.67171638009516 37.0 37.0 37.0 37.0 37.0 5 36.67001369874779 37.0 37.0 37.0 37.0 37.0 6 36.54090300084168 37.0 37.0 37.0 37.0 37.0 7 36.48724653022313 37.0 37.0 37.0 37.0 37.0 8 36.4131287181293 37.0 37.0 37.0 37.0 37.0 9 36.416509567652064 37.0 37.0 37.0 37.0 37.0 10-11 36.36241302297152 37.0 37.0 37.0 37.0 37.0 12-13 36.34587369954939 37.0 37.0 37.0 37.0 37.0 14-15 38.15271876127248 40.0 37.0 40.0 37.0 40.0 16-17 38.09168829909362 40.0 37.0 40.0 37.0 40.0 18-19 38.13152810819864 40.0 37.0 40.0 37.0 40.0 20-21 38.0670519350018 40.0 37.0 40.0 37.0 40.0 22-23 37.97394536103429 40.0 37.0 40.0 33.0 40.0 24-25 37.931244667937776 40.0 37.0 40.0 33.0 40.0 26-27 37.811461053042926 40.0 37.0 40.0 33.0 40.0 28-29 37.699223290304666 40.0 37.0 40.0 33.0 40.0 30-31 37.67210966441647 40.0 37.0 40.0 33.0 40.0 32-33 37.53868840573487 40.0 37.0 40.0 33.0 40.0 34-35 37.4368079949442 40.0 37.0 40.0 33.0 40.0 36-37 37.30227281836348 40.0 37.0 40.0 33.0 40.0 38-39 37.193244044551705 40.0 37.0 40.0 33.0 40.0 40-41 37.121542569180825 40.0 37.0 40.0 33.0 40.0 42-43 36.969191505488084 37.0 37.0 40.0 33.0 40.0 44-45 36.82022066506919 37.0 37.0 40.0 33.0 40.0 46-47 36.73769224052539 37.0 37.0 40.0 33.0 40.0 48-49 36.51851147073306 37.0 37.0 40.0 33.0 40.0 50-51 36.331737472158764 37.0 37.0 40.0 33.0 40.0 52-53 36.15549805397622 37.0 37.0 40.0 33.0 40.0 54-55 35.976841851750066 37.0 37.0 40.0 33.0 40.0 56-57 35.757007188621884 37.0 35.0 40.0 30.0 40.0 58-59 35.50201598473527 37.0 33.0 40.0 27.0 40.0 60-61 35.22062785211651 37.0 33.0 40.0 27.0 40.0 62-63 34.9471147996576 37.0 33.0 37.0 27.0 40.0 64-65 34.650948266113566 37.0 33.0 37.0 27.0 40.0 66-67 34.304536081957735 37.0 33.0 37.0 27.0 40.0 68-69 33.99512867804937 37.0 33.0 37.0 27.0 38.5 70-71 33.61778954887175 37.0 33.0 37.0 27.0 37.0 72-73 33.28659897094778 37.0 33.0 37.0 27.0 37.0 74-75 32.906088248850565 37.0 33.0 37.0 27.0 37.0 76-77 30.13883893808223 33.0 30.0 33.0 22.0 37.0 78-79 32.714571309354085 33.0 33.0 37.0 27.0 37.0 80-81 33.23515423258384 37.0 33.0 37.0 27.0 37.0 82-83 33.16837650514455 37.0 33.0 37.0 27.0 37.0 84-85 32.99237738761874 37.0 33.0 37.0 27.0 37.0 86-87 32.814503907077544 33.0 33.0 37.0 27.0 37.0 88-89 32.69465605693641 33.0 33.0 37.0 27.0 37.0 90-91 32.544758010260466 33.0 33.0 37.0 27.0 37.0 92-93 32.38007351088743 33.0 33.0 37.0 22.0 37.0 94-95 32.161072653463194 33.0 33.0 37.0 22.0 37.0 96-97 31.991484625052248 33.0 33.0 37.0 22.0 37.0 98-99 31.83489108278796 33.0 33.0 37.0 22.0 37.0 100-101 30.130755317032254 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14712848395351585 2306 2 0.13997316840309537 2306 3 0.11410148202494952 2306 4 0.17017930617756605 2306 5 0.18030906631597077 2306 6 0.19502589716169183 2306 7 0.14980358043529662 2306 8 0.18945730102995384 2306 9 0.2090415245374473 2306 10-11 0.2153769110508179 2306 12-13 0.2815326925440047 2306 14-15 0.44976591706571156 2306 16-17 0.40038447350165285 2306 18-19 0.3444855673656235 2306 20-21 0.3927107041545739 2306 22-23 0.38965430853156136 2306 24-25 0.43217176563969417 2306 26-27 0.32976607777042233 2306 28-29 0.38732231816636187 2306 30-31 0.3891837613270184 2306 32-33 0.40291542748040854 2306 34-35 0.44033129699999307 2306 36-37 0.3371240052310185 2306 38-39 0.31328140859655207 2306 40-41 0.3762287373603499 2306 42-43 0.4203848013278062 2306 44-45 0.44989352591895937 2306 46-47 0.3506361788157122 2306 48-49 0.2753369607363112 2306 50-51 0.37445796543168086 2306 52-53 0.3902608107327552 2306 54-55 0.3682027232043268 2306 56-57 0.3673233512124696 2306 58-59 0.22952469040993861 2306 60-61 0.3535547937591801 2306 62-63 0.2297137435260126 2306 64-65 0.24747853655567553 2306 66-67 0.2642014098536265 2306 68-69 0.08732601975764709 2306 70-71 0.35365909904729165 2306 72-73 0.23050462710560993 2306 74-75 0.232844672430069 2306 76-77 -0.2521940537003786 2306 78-79 0.10543660707509872 2306 80-81 0.24178977619038733 2306 82-83 0.38895639412528027 2306 84-85 0.3684892040016905 2306 86-87 0.44937211072697636 2306 88-89 0.3555886867582956 2306 90-91 0.2702992133981823 2306 92-93 0.24779310216168682 2306 94-95 0.2904301721355864 2306 96-97 0.3701023934458334 2306 98-99 0.3170208183731482 2306 100-101 0.12410241823964796 2307 1 0.13132686782783054 2307 2 0.122417259833 2307 3 0.12187910400771784 2307 4 0.15066429525556657 2307 5 0.11164920363582809 2307 6 0.16121914780419644 2307 7 0.1429002011892706 2307 8 0.19160261793299327 2307 9 0.17772283386193521 2307 10-11 0.23421651905548657 2307 12-13 0.24308343304045366 2307 14-15 0.419186114506644 2307 16-17 0.40733215536864265 2307 18-19 0.3108577249104414 2307 20-21 0.3589292053336166 2307 22-23 0.45032283312443866 2307 24-25 0.37846925728270975 2307 26-27 0.4384760643967667 2307 28-29 0.4785435026227063 2307 30-31 0.5085430801810773 2307 32-33 0.47079376321820376 2307 34-35 0.4908846654443977 2307 36-37 0.43037214877829655 2307 38-39 0.45526638263514485 2307 40-41 0.35818530421910566 2307 42-43 0.25596237668516864 2307 44-45 0.3508710348680566 2307 46-47 0.30730801977622235 2307 48-49 0.4252515493284079 2307 50-51 0.3929112467519573 2307 52-53 0.40585500707635447 2307 54-55 0.20475733773335492 2307 56-57 0.2842770116496425 2307 58-59 0.25741547394452624 2307 60-61 0.3586312934241036 2307 62-63 0.3004738375258711 2307 64-65 0.3415697022150539 2307 66-67 0.2992086036466617 2307 68-69 0.2656896440565504 2307 70-71 0.34295665587193014 2307 72-73 0.3246499150390463 2307 74-75 0.23982210686724414 2307 76-77 -0.24207664653508587 2307 78-79 -0.037671298124074326 2307 80-81 0.3279147564287399 2307 82-83 0.41094161871183843 2307 84-85 0.38447383758214926 2307 86-87 0.1716821081060118 2307 88-89 0.2705788733036485 2307 90-91 0.28424811218445 2307 92-93 0.19109429467210504 2307 94-95 0.35398515276477127 2307 96-97 0.4100230676129826 2307 98-99 0.24910724932854933 2307 100-101 0.13334055865964345 2201 1 0.15280295404993183 2201 2 0.11884485887105001 2201 3 0.08827213344412144 2201 4 0.12190800418576231 2201 5 0.08829076505106315 2201 6 0.08629947487163037 2201 7 0.15538062762242788 2201 8 0.13325999772309416 2201 9 0.15093284145515184 2201 10-11 0.1326426078796814 2201 12-13 0.11769151533639644 2201 14-15 0.2756254133407765 2201 16-17 0.2588592199427211 2201 18-19 0.2522088529081188 2201 20-21 0.21773366483740375 2201 22-23 0.2570114214444672 2201 24-25 0.21425445872075954 2201 26-27 0.18596550510542187 2201 28-29 0.24855827049801604 2201 30-31 0.25056977686078596 2201 32-33 0.33718854299936396 2201 34-35 0.3276687927327515 2201 36-37 0.23259926051802182 2201 38-39 0.23412686019593565 2201 40-41 0.2009894174004998 2201 42-43 0.21310820013133736 2201 44-45 0.20433837709365577 2201 46-47 0.14107391461890728 2201 48-49 0.22250636481075503 2201 50-51 0.17458047151723832 2201 52-53 0.12747417666480487 2201 54-55 0.1923320266710462 2201 56-57 0.1817422548664851 2201 58-59 0.19226199300347702 2201 60-61 0.13859823473878663 2201 62-63 0.038708579129611564 2201 64-65 -0.012986307617083526 2201 66-67 0.060403273068303065 2201 68-69 0.1286033452108839 2201 70-71 0.13267398338935976 2201 72-73 0.029069839956868293 2201 74-75 0.060270424601533534 2201 76-77 -0.0853058476244648 2201 78-79 0.13895493753005184 2201 80-81 0.11083140916777268 2201 82-83 0.016638294268574327 2201 84-85 0.14178575645232172 2201 86-87 0.02622122916014291 2201 88-89 -0.02279225498790538 2201 90-91 -0.08317117540884311 2201 92-93 0.03221594017882268 2201 94-95 -0.021659145334986363 2201 96-97 0.12777751695715267 2201 98-99 -0.011566151033424177 2201 100-101 -0.08103944259194051 1101 1 0.09684046090887932 1101 2 0.08571963598132726 1101 3 0.08084787001374139 1101 4 0.13405925224291337 1101 5 0.09843793699288739 1101 6 0.14140052795508495 1101 7 0.10632202610456432 1101 8 0.0660622967339819 1101 9 0.07304415936709319 1101 10-11 0.11667488231110923 1101 12-13 0.10427561606459079 1101 14-15 0.1545542230950545 1101 16-17 0.15926003516578646 1101 18-19 0.04443290917112108 1101 20-21 0.12237911984980343 1101 22-23 0.1849486172809094 1101 24-25 0.16758182118474707 1101 26-27 0.1020430364853695 1101 28-29 0.16161984819392927 1101 30-31 0.16761374658202755 1101 32-33 0.2743191828922633 1101 34-35 0.21419524488798203 1101 36-37 0.0440627972214358 1101 38-39 0.10007881644732208 1101 40-41 0.1534713387996618 1101 42-43 0.20384815015393798 1101 44-45 0.03366054805413654 1101 46-47 0.1839386091796058 1101 48-49 0.10618314318884359 1101 50-51 0.11977937997356491 1101 52-53 0.18349641155862173 1101 54-55 0.15265692575993484 1101 56-57 0.09688372772175313 1101 58-59 0.03938485733367969 1101 60-61 0.030549859213351738 1101 62-63 -0.028153743299839107 1101 64-65 0.019476379495444007 1101 66-67 0.12187406543238666 1101 68-69 0.008970498344368139 1101 70-71 -0.044496252575164874 1101 72-73 -0.04478983640099443 1101 74-75 0.005785632883878122 1101 76-77 -0.07461943393780501 1101 78-79 0.04487945068043331 1101 80-81 0.20105536687907488 1101 82-83 0.22632902773548835 1101 84-85 0.35905244363667776 1101 86-87 0.2666727275219358 1101 88-89 0.231580820435056 1101 90-91 0.1407353286697699 1101 92-93 0.08066890282688632 1101 94-95 0.08981990491052017 1101 96-97 0.11762319375264951 1101 98-99 0.06413930797950584 1101 100-101 0.029532728368547367 2304 1 0.08261451201637016 2304 2 0.10259751479445356 2304 3 0.08147867971154454 2304 4 0.08195794182503136 2304 5 0.09140232600013576 2304 6 0.012073381714351683 2304 7 0.04591753426343104 2304 8 0.04657191360635693 2304 9 0.09005965586193554 2304 10-11 0.01289180943474122 2304 12-13 0.06914293774183733 2304 14-15 0.12402987741133842 2304 16-17 0.08239572471219958 2304 18-19 0.11266301188597794 2304 20-21 0.06866768255585498 2304 22-23 0.017870709307707955 2304 24-25 0.011373478428943429 2304 26-27 0.06812548509818583 2304 28-29 0.09112240118788861 2304 30-31 0.05624607974476703 2304 32-33 0.15709086251234083 2304 34-35 0.13557314750524796 2304 36-37 0.07359927111424014 2304 38-39 0.043815501006776 2304 40-41 0.08848417815742948 2304 42-43 0.0030821775763030246 2304 44-45 0.10804513665909354 2304 46-47 -0.024042621256370467 2304 48-49 0.012002065099359527 2304 50-51 0.008998633951826207 2304 52-53 0.0694355294962321 2304 54-55 0.04537910764737063 2304 56-57 0.04928697877210908 2304 58-59 0.05236257665907118 2304 60-61 -0.04438966339463235 2304 62-63 0.009082253915075 2304 64-65 -0.056693147635328955 2304 66-67 -0.09664867942294819 2304 68-69 -0.15039858781471338 2304 70-71 -0.17776819106526887 2304 72-73 -0.07453013323861768 2304 74-75 -0.07560890287588506 2304 76-77 -0.48314161344482187 2304 78-79 -0.08451278500434256 2304 80-81 0.04565078577242332 2304 82-83 0.26464625360106453 2304 84-85 0.22267518075565818 2304 86-87 0.02793134164492983 2304 88-89 -0.00951494361831351 2304 90-91 -0.05864256502093923 2304 92-93 -0.022359876377812782 2304 94-95 -0.06962245336558226 2304 96-97 0.01913393834613686 2304 98-99 0.07021791817481926 2304 100-101 0.0797743403848763 2202 1 0.04372967721248244 2202 2 0.06735977733958265 2202 3 0.044501712085860845 2202 4 0.06475222961002913 2202 5 0.07992570080149619 2202 6 0.047027619498351214 2202 7 0.07036782250056461 2202 8 0.1383968835193201 2202 9 0.07558222280143667 2202 10-11 0.06525696631951661 2202 12-13 0.10795085113105785 2202 14-15 0.062131535024192885 2202 16-17 0.004886366147871968 2202 18-19 -0.033989306644308215 2202 20-21 -0.010919177554384873 2202 22-23 -0.013619405189253087 2202 24-25 -0.07276055516215507 2202 26-27 0.04278285027169204 2202 28-29 0.037398008414101014 2202 30-31 0.024933661611427738 2202 32-33 0.011924777085106086 2202 34-35 -0.07587434646072211 2202 36-37 -0.13040737463001761 2202 38-39 0.05429696838976383 2202 40-41 -0.06831657252243417 2202 42-43 -0.15738790327637275 2202 44-45 -0.09636897233244213 2202 46-47 -0.13569532210811275 2202 48-49 -0.25661524442143957 2202 50-51 -0.07779261687099392 2202 52-53 0.03530685671685774 2202 54-55 -0.030818555869849718 2202 56-57 -0.031123451622107723 2202 58-59 -0.0022586092688356985 2202 60-61 0.009882404864711702 2202 62-63 -0.198242817396185 2202 64-65 -0.19555491848181816 2202 66-67 -0.13753920582973223 2202 68-69 -0.11358182119038673 2202 70-71 -0.06528813827166147 2202 72-73 -0.25777734727878965 2202 74-75 -0.15180565214615171 2202 76-77 -0.5359429076860636 2202 78-79 -0.25617923280583454 2202 80-81 -0.0267757337060317 2202 82-83 0.08893073068504975 2202 84-85 0.02114793120945535 2202 86-87 -0.006005173046190748 2202 88-89 -0.008034777790541625 2202 90-91 -0.08964829485597647 2202 92-93 -0.10819864809533897 2202 94-95 -0.10293079728202059 2202 96-97 0.02653281432105814 2202 98-99 -0.058488979236695826 2202 100-101 0.023123983329881526 1102 1 0.11123703103901761 1102 2 0.07221603942532084 1102 3 0.042851012523975385 1102 4 -0.01545647449720633 1102 5 0.04063265708921193 1102 6 -0.00466454383077064 1102 7 -0.007050145484200243 1102 8 -0.02364759538284744 1102 9 8.658730925859004E-4 1102 10-11 0.005665209852431019 1102 12-13 0.03419064041887765 1102 14-15 -0.011383290207064078 1102 16-17 -0.056803717387374775 1102 18-19 -0.06886867671210695 1102 20-21 -0.15126666921350562 1102 22-23 -0.13227177802269097 1102 24-25 -0.06587409718700599 1102 26-27 -0.05822316980681563 1102 28-29 -0.17635671405798092 1102 30-31 -0.13815054437347385 1102 32-33 -0.08109802402356081 1102 34-35 -0.09034229479879485 1102 36-37 -0.10426891395680826 1102 38-39 -0.061526998007408906 1102 40-41 -0.09918560174939017 1102 42-43 -0.19181283272002503 1102 44-45 -0.22761749788691787 1102 46-47 -0.20045444158906633 1102 48-49 -0.188656790474532 1102 50-51 -0.1604275647308242 1102 52-53 -0.17628893779649246 1102 54-55 -0.1700734196999676 1102 56-57 -0.2636237790669682 1102 58-59 -0.21846284578254682 1102 60-61 -0.18057551214811696 1102 62-63 -0.18059521902966935 1102 64-65 -0.203942074188177 1102 66-67 -0.19536474169748885 1102 68-69 -0.20069953639148963 1102 70-71 -0.3819358313801473 1102 72-73 -0.29296704448571376 1102 74-75 -0.4432891981833862 1102 76-77 -0.5199633153332144 1102 78-79 -0.36186241112343254 1102 80-81 -0.21247080950149666 1102 82-83 -0.1106777886942183 1102 84-85 -0.2081025390089124 1102 86-87 -0.141866821080896 1102 88-89 -0.21598647901286228 1102 90-91 -0.12017819689428677 1102 92-93 -0.19283761512853204 1102 94-95 -0.29258363401140386 1102 96-97 -0.2759156994567249 1102 98-99 -0.3599436648311247 1102 100-101 -0.4773013741512031 2305 1 0.002124321858012479 2305 2 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-0.1009331660636974 1213 88-89 -0.29302885036099724 1213 90-91 -0.14469207996462075 1213 92-93 -0.06658694620526973 1213 94-95 0.10871234399049712 1213 96-97 -0.051655475141973994 1213 98-99 -0.08725386439409277 1213 100-101 0.2755648589221771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 18.0 9 60.0 10 47.0 11 40.0 12 64.0 13 167.0 14 473.0 15 1147.0 16 2417.0 17 4186.0 18 6055.0 19 8319.0 20 10944.0 21 14274.0 22 18436.0 23 23434.0 24 29727.0 25 37697.0 26 48313.0 27 61800.0 28 82604.0 29 107078.0 30 123164.0 31 148055.0 32 200130.0 33 283788.0 34 433770.0 35 739767.0 36 1332439.0 37 1609326.0 38 261035.0 39 58.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.98465743222769 24.704134244914467 22.919522973532597 15.391685349325247 2 31.67635026424126 21.96721962656956 32.53302300015459 13.823407109034589 3 39.462840602904585 18.788500622523284 24.90248805324319 16.846170721328946 4 31.24112457376481 18.23964509747222 32.7946104689947 17.724619859768264 5 34.31822606226131 16.79492244533384 31.389170402688794 17.497681089716064 6 28.058528150425705 17.794254685057627 36.434840052447456 17.712377112069213 7 34.509929802864 18.64198100783849 27.51075716715049 19.337332022147024 8 30.10800825646575 25.99918909711367 24.89767092659074 18.995131719829832 9 31.658725830370283 23.664998339545722 24.841666380381447 19.83460944970255 10-11 26.183028940572918 27.984836545453508 28.35318721335692 17.47894730061666 12-13 25.84487734371547 26.2527087516902 27.970939887407543 19.931474017186783 14-15 25.453091093094226 26.756350879754482 27.717481935402606 20.07307609174869 16-17 24.85984771593132 27.072698470154272 28.539293052581744 19.528160761332668 18-19 23.395067162512667 27.20714274467366 29.208276434145812 20.189513658667856 20-21 23.37444568024231 27.83521673222598 29.022146308924658 19.76819127860705 22-23 23.216129476982054 28.467100455042104 28.44799088752758 19.868779180448264 24-25 23.49510067314638 26.785512671795825 29.463282916689703 20.256103738368093 26-27 22.632072318509486 26.6723351140274 29.362816774596194 21.33277579286692 28-29 22.38715531259483 27.88811687307831 28.34557381578119 21.37915399854567 30-31 20.96002348970232 28.453387040440646 28.82054783539745 21.76604163445958 32-33 20.991586435233696 28.089429061385278 28.880023589902148 22.03896091347888 34-35 21.996152326639983 28.119265349182083 29.01666215767445 20.867920166503485 36-37 21.3100161178579 29.359810779783686 29.316402783264913 20.013770319093506 38-39 21.266081893580484 28.575390593023254 28.826653056057427 21.331874457338838 40-41 21.726453756348374 27.820821953495827 27.793428036484187 22.659296253671606 42-43 22.389284558920362 28.45056892030392 28.298184665418464 20.861961855357254 44-45 21.27525530406565 28.6382417344895 28.259494927915092 21.82700803352976 46-47 22.042298159123348 28.357203341875746 28.43663037220573 21.163868126795172 48-49 22.09366866368039 28.305737063398535 28.734040501170405 20.86655377175067 50-51 21.771307308711517 28.30287664100229 27.589084259439844 22.33673179084635 52-53 21.074881171954793 28.447838406741543 27.834293118959742 22.642987302343922 54-55 22.038633964510606 28.44255717826927 28.302926798413257 21.215882058806873 56-57 21.967924139367465 28.224925043356397 27.601571709655648 22.20557910762049 58-59 21.377837611165038 27.885893511502186 28.19032325686325 22.54594562046953 60-61 21.09023022554112 27.87412433256387 28.909215087415994 22.12643035447902 62-63 20.801361156109515 27.940810321969284 28.821101068865048 22.436727453056154 64-65 21.90929098635652 28.42487139342429 28.26539029299933 21.40044732721986 66-67 22.14579809248951 28.729606388741107 27.73865757056982 21.385937948199558 68-69 21.77002916317915 27.90622331238064 28.647985351754286 21.675762172685925 70-71 21.38688153996427 29.274318572306303 28.39850433554263 20.940295552186804 72-73 22.36617711148575 29.555740997868725 27.64330227726322 20.434779613382304 74-75 23.372585707020512 28.481007221897535 27.452632132737754 20.693774938344202 76-77 23.404996580044187 27.22060258324092 27.89445689804416 21.479943938670733 78-79 23.072471672467806 27.41102531673656 28.62337784572616 20.893125165069474 80-81 22.1853661783549 28.48363424052523 28.227591299675364 21.103408281444505 82-83 21.345551270820092 28.442597666202886 28.578359485488203 21.63349157748882 84-85 21.570127999623534 27.951491602275297 28.744299654013723 21.734080744087443 86-87 20.76018855753841 28.593244100419685 29.248927979358513 21.397639362683393 88-89 20.718254436191614 29.138272899037148 29.15785768721277 20.985614977558463 90-91 21.437007239971226 28.2538356100651 28.40702738877894 21.902129761184735 92-93 21.36225815999207 28.613521602805285 27.61918557730058 22.40503465990206 94-95 21.088985049563984 29.58852480489706 27.138670358167698 22.18381978737126 96-97 21.119122736960758 30.4088875869388 26.417100251539022 22.054889424561424 98-99 21.352967423180495 29.078808322740663 27.303605493224282 22.264618760854564 100-101 21.953607522889666 29.075209481380533 27.057247045188106 21.9139359505417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 131.5 2 139.5 3 289.0 4 287.0 5 185.0 6 172.5 7 186.0 8 193.0 9 191.5 10 186.5 11 184.0 12 184.5 13 171.5 14 169.0 15 208.0 16 213.5 17 211.5 18 248.5 19 281.0 20 318.5 21 403.0 22 547.0 23 736.0 24 1002.5 25 1393.0 26 1945.5 27 2735.0 28 3667.0 29 4865.0 30 6542.5 31 9297.5 32 15393.5 33 27071.5 34 46131.0 35 78656.5 36 123070.5 37 187185.0 38 270683.0 39 322950.0 40 341679.0 41 333881.0 42 343329.5 43 386532.5 44 384088.5 45 365086.5 46 378517.5 47 364376.5 48 301593.0 49 245121.5 50 198795.0 51 164144.5 52 147340.0 53 134089.0 54 106797.5 55 67667.5 56 43490.5 57 34503.0 58 30347.5 59 26388.5 60 21428.5 61 16664.5 62 11961.0 63 8097.0 64 5729.0 65 4205.0 66 3367.5 67 2891.5 68 2340.5 69 1737.0 70 1238.5 71 869.5 72 623.0 73 479.5 74 320.0 75 189.0 76 135.0 77 98.0 78 60.0 79 34.0 80 19.5 81 14.5 82 11.5 83 8.0 84 6.0 85 3.0 86 2.5 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14931563518101815 2 0.0 3 0.005117348311776056 4 0.006584560065502058 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.367847879485373E-5 14-15 0.0 16-17 8.946413132475623E-6 18-19 0.0 20-21 0.0 22-23 1.520890232520856E-4 24-25 5.367847879485373E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.751590314398429E-4 40-41 0.0 42-43 0.0 44-45 1.0735695758970746E-4 46-47 9.751590314398429E-4 48-49 0.002755495244802492 50-51 3.936421778289274E-4 52-53 0.0015029974062559046 54-55 0.0026570847003452597 56-57 0.004795277439006934 58-59 0.006271435605865411 60-61 0.0016998184951703683 62-63 9.572662051748917E-4 64-65 0.0014672117537260021 66-67 0.008964305958740574 68-69 0.008042825406095584 70-71 0.009778429553795855 72-73 0.007005041482728413 74-75 0.009841054445723186 76-77 0.005537829729002411 78-79 0.004938420049126544 80-81 0.0021650319780591007 82-83 0.0 84-85 4.74159896021208E-4 86-87 0.0015566758850507584 88-89 0.005564668968399838 90-91 0.007371844421159913 92-93 0.009483197920424161 94-95 0.008946413132475623 96-97 0.011335105438846613 98-99 0.008168075189950243 100-101 0.008007039753565683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5588832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.062542025339837 #Duplication Level Percentage of deduplicated Percentage of total 1 82.58639437501722 24.82756951620245 2 7.687177091591321 4.6219216874438755 3 2.730274905288786 2.4623701224292467 4 1.2005406103065264 1.4436521020186837 5 0.9063411685375197 1.3623459734227399 6 0.5726844473971262 1.0329810160280777 7 0.4470836008524123 0.9408328677626137 8 0.43886829819302414 1.0554797326413732 9 0.35599425744481916 0.9631883092693073 >10 2.394031943328995 14.387312408509695 >50 0.3133131379208977 6.644680350574415 >100 0.3094855172349414 18.997700478553835 >500 0.03640959654770284 7.738212609092113 >1k 0.020684174928508128 10.634561516603434 >5k 4.1818209174446057E-4 0.8450239923488084 >10k+ 2.986933184732586E-4 2.0421673170994703 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 42817 0.7661171421864175 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23683 0.42375580443284033 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 23392 0.4185489919897396 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12990 0.23242781318171668 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10831 0.19379720127568695 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8060 0.14421617969550704 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7857 0.14058393596372193 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7215 0.12909674150162323 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6741 0.12061554185203635 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6036 0.10800109933524571 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6004 0.10742852889476728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.083560930083423E-4 0.0 0.0 2 6.799273980681473E-4 0.0 0.0 3 7.157130505980498E-4 0.0 0.0 4 7.514987031279523E-4 0.0 0.0 5 7.693915293929036E-4 0.0 0.0 6 8.76748486982611E-4 0.0 1.7892826264951246E-5 7 0.001019891097102221 0.0 1.7892826264951246E-5 8 0.0011451408809568797 0.0 1.7892826264951246E-5 9 0.0012703906648115384 0.0 1.7892826264951246E-5 10-11 0.0022902817619137594 0.0 1.7892826264951246E-5 12-13 0.0032386015539561754 0.0 1.7892826264951246E-5 14-15 0.004580563523827519 0.0 1.7892826264951246E-5 16-17 0.006146185822010753 0.0 1.7892826264951246E-5 18-19 0.007828111490916171 0.0 1.7892826264951246E-5 20-21 0.009930518577047942 0.0 1.7892826264951246E-5 22-23 0.012462353493538542 0.0 1.7892826264951246E-5 24-25 0.01535204493532817 0.0 1.7892826264951246E-5 26-27 0.018268575616515223 0.0 1.7892826264951246E-5 28-29 0.021703998259385862 8.946413132475623E-6 1.7892826264951246E-5 30-31 0.03382638805389033 1.7892826264951246E-5 1.7892826264951246E-5 32-33 0.038049095052418824 1.7892826264951246E-5 1.7892826264951246E-5 34-35 0.041922891938780765 1.7892826264951246E-5 1.7892826264951246E-5 36-37 0.046270848721163924 1.7892826264951246E-5 1.7892826264951246E-5 38-39 0.05129873290161522 1.7892826264951246E-5 1.7892826264951246E-5 40-41 0.05669342002049802 1.7892826264951246E-5 1.7892826264951246E-5 42-43 0.06284855225564125 1.7892826264951246E-5 1.7892826264951246E-5 44-45 0.07008620047981404 1.7892826264951246E-5 1.7892826264951246E-5 46-47 0.07783379425253792 1.7892826264951246E-5 3.578565252990249E-5 48-49 0.08710227825778266 1.7892826264951246E-5 3.578565252990249E-5 50-51 0.09698806476916821 1.7892826264951246E-5 3.578565252990249E-5 52-53 0.10798320650898077 1.7892826264951246E-5 3.578565252990249E-5 54-55 0.12046345282878426 1.7892826264951246E-5 3.578565252990249E-5 56-57 0.13518924884483913 1.7892826264951246E-5 3.578565252990249E-5 58-59 0.15100650726305603 1.7892826264951246E-5 3.578565252990249E-5 60-61 0.17049179506558793 1.7892826264951246E-5 3.578565252990249E-5 62-63 0.19173057984208508 1.7892826264951246E-5 3.578565252990249E-5 64-65 0.21585010964723936 1.7892826264951246E-5 3.578565252990249E-5 66-67 0.24141895837985466 1.7892826264951246E-5 3.578565252990249E-5 68-69 0.2708973896513619 1.7892826264951246E-5 6.262489192732936E-5 70-71 0.30429434987489334 1.7892826264951246E-5 8.05177181922806E-5 72-73 0.3419498027494833 1.7892826264951246E-5 8.946413132475623E-5 74-75 0.38371165925187944 1.7892826264951246E-5 8.946413132475623E-5 76-77 0.43176284418640604 1.7892826264951246E-5 8.946413132475623E-5 78-79 0.48664908875414403 1.7892826264951246E-5 8.946413132475623E-5 80-81 0.5464379677184785 1.7892826264951246E-5 8.946413132475623E-5 82-83 0.6143412433939686 1.7892826264951246E-5 1.0735695758970747E-4 84-85 0.6919961093838569 1.7892826264951246E-5 1.163033707221831E-4 86-87 0.7756719114119015 1.7892826264951246E-5 1.2524978385465872E-4 88-89 0.8664153798146017 1.7892826264951246E-5 1.3419619698713434E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGG 30 1.290329E-4 63.445385 1 TTGTGTT 62735 0.0 46.389416 1 CGGAAGA 10995 0.0 42.25155 4 GAAGAGC 11095 0.0 41.95373 6 CGGACCG 80 2.2334825E-7 41.636032 1 GCCGTCT 5545 0.0 41.244553 50-51 TCGGAAG 11285 0.0 41.127365 3 TATGCCG 5605 0.0 40.84541 48-49 CGTATGC 5690 0.0 40.48567 46-47 TGCCGTC 5640 0.0 40.465614 50-51 TCGTATG 5740 0.0 40.298504 44-45 ATGCCGT 5700 0.0 40.206318 48-49 CTCGTAT 5650 0.0 40.141796 44-45 TCTCGTA 5675 0.0 40.00681 42-43 CATCTCG 5655 0.0 39.68634 40-41 CGACGCC 120 8.54925E-11 39.653366 1 ATCTCGT 5785 0.0 38.999775 42-43 GATCGGA 12055 0.0 38.84089 1 CTGCTTG 6020 0.0 38.267384 58-59 CTTGAAA 5775 0.0 38.16464 62-63 >>END_MODULE