FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588832
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT428170.7661171421864175TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC236830.42375580443284033No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA233920.4185489919897396No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT129900.23242781318171668No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC108310.19379720127568695No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC80600.14421617969550704No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT78570.14058393596372193No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC72150.12909674150162323No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA67410.12061554185203635No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT60360.10800109933524571No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60040.10742852889476728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGG301.290329E-463.4453851
TTGTGTT627350.046.3894161
CGGAAGA109950.042.251554
GAAGAGC110950.041.953736
CGGACCG802.2334825E-741.6360321
GCCGTCT55450.041.24455350-51
TCGGAAG112850.041.1273653
TATGCCG56050.040.8454148-49
CGTATGC56900.040.4856746-47
TGCCGTC56400.040.46561450-51
TCGTATG57400.040.29850444-45
ATGCCGT57000.040.20631848-49
CTCGTAT56500.040.14179644-45
TCTCGTA56750.040.0068142-43
CATCTCG56550.039.6863440-41
CGACGCC1208.54925E-1139.6533661
ATCTCGT57850.038.99977542-43
GATCGGA120550.038.840891
CTGCTTG60200.038.26738458-59
CTTGAAA57750.038.1646462-63
Produced by FastQC (version 0.11.2)