##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8114130 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66566421785207 33.0 33.0 33.0 33.0 33.0 2 32.69760257723256 33.0 33.0 33.0 33.0 33.0 3 32.73304038757082 33.0 33.0 33.0 33.0 33.0 4 36.66562391778293 37.0 37.0 37.0 37.0 37.0 5 36.6727739141473 37.0 37.0 37.0 37.0 37.0 6 36.55122902886693 37.0 37.0 37.0 37.0 37.0 7 36.45292027611093 37.0 37.0 37.0 37.0 37.0 8 36.39405062526728 37.0 37.0 37.0 37.0 37.0 9 36.44539808950559 37.0 37.0 37.0 37.0 37.0 10-11 36.37154057181731 37.0 37.0 37.0 37.0 37.0 12-13 36.36029919412186 37.0 37.0 37.0 37.0 37.0 14-15 38.21433277504797 40.0 37.0 40.0 37.0 40.0 16-17 38.21206833018451 40.0 37.0 40.0 37.0 40.0 18-19 38.19167686492575 40.0 37.0 40.0 37.0 40.0 20-21 38.192856350588414 40.0 37.0 40.0 37.0 40.0 22-23 38.029526085975945 40.0 37.0 40.0 35.0 40.0 24-25 38.052129371848864 40.0 37.0 40.0 35.0 40.0 26-27 37.98240199503829 40.0 37.0 40.0 35.0 40.0 28-29 37.814931853445785 40.0 37.0 40.0 33.0 40.0 30-31 37.820482294466565 40.0 37.0 40.0 33.0 40.0 32-33 37.67148197034063 40.0 37.0 40.0 33.0 40.0 34-35 37.51727326281437 40.0 37.0 40.0 33.0 40.0 36-37 37.229461199167375 40.0 37.0 40.0 33.0 40.0 38-39 37.35840502925144 40.0 37.0 40.0 33.0 40.0 40-41 37.17488775752915 38.5 37.0 40.0 33.0 40.0 42-43 36.993417224027716 37.0 37.0 40.0 33.0 40.0 44-45 36.98957941270352 37.0 37.0 40.0 33.0 40.0 46-47 36.99892674877036 37.0 37.0 40.0 33.0 40.0 48-49 36.698559241717845 37.0 37.0 40.0 33.0 40.0 50-51 36.59193856889155 37.0 37.0 40.0 33.0 40.0 52-53 36.492194603734475 37.0 37.0 40.0 33.0 40.0 54-55 36.23666412788555 37.0 37.0 40.0 33.0 40.0 56-57 36.103755917147 37.0 37.0 40.0 33.0 40.0 58-59 35.83769547690264 37.0 37.0 40.0 33.0 40.0 60-61 35.549454778269514 37.0 35.0 40.0 27.0 40.0 62-63 35.315827698101955 37.0 33.0 38.5 27.0 40.0 64-65 35.02608203220801 37.0 33.0 37.0 27.0 40.0 66-67 34.481540041877565 37.0 33.0 37.0 27.0 40.0 68-69 34.294425095481586 37.0 33.0 37.0 27.0 40.0 70-71 33.807812421048226 37.0 33.0 37.0 27.0 38.5 72-73 33.06845028364101 37.0 33.0 37.0 24.5 37.0 74-75 32.370930648140956 37.0 33.0 37.0 22.0 37.0 76-77 29.768274848936365 33.0 27.0 35.0 18.5 37.0 78-79 31.9955783922614 33.0 33.0 37.0 22.0 37.0 80-81 32.34589000915686 37.0 33.0 37.0 22.0 37.0 82-83 32.27438228127969 37.0 33.0 37.0 22.0 37.0 84-85 32.10114898331676 37.0 33.0 37.0 18.5 37.0 86-87 31.94317141825433 35.0 33.0 37.0 15.0 37.0 88-89 31.812462642328875 33.0 33.0 37.0 15.0 37.0 90-91 31.65587660044885 33.0 33.0 37.0 15.0 37.0 92-93 31.513807888214757 33.0 33.0 37.0 15.0 37.0 94-95 31.3347823488162 33.0 33.0 37.0 15.0 37.0 96-97 31.184651712506454 33.0 33.0 37.0 15.0 37.0 98-99 31.047111212169387 33.0 33.0 37.0 15.0 37.0 100-101 29.52440600532651 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16127748228021943 2306 2 0.1456601523814811 2306 3 0.12926561807307024 2306 4 0.16728159112624041 2306 5 0.14606925719942865 2306 6 0.14812593091328807 2306 7 0.19103065799468766 2306 8 0.2453529164057855 2306 9 0.18298139701818883 2306 10-11 0.22282612379044764 2306 12-13 0.2279211196139741 2306 14-15 0.40097469021581134 2306 16-17 0.3605851205498283 2306 18-19 0.41700182079768666 2306 20-21 0.3851104368441227 2306 22-23 0.4255591195529078 2306 24-25 0.4569369286282594 2306 26-27 0.4113206583772069 2306 28-29 0.4519575892131229 2306 30-31 0.43226602628026 2306 32-33 0.42242752498037817 2306 34-35 0.5234736832907245 2306 36-37 0.39972935240397334 2306 38-39 0.37134217702352856 2306 40-41 0.4960673452580622 2306 42-43 0.48224540258294013 2306 44-45 0.3934299696359034 2306 46-47 0.4038073896132417 2306 48-49 0.36294020099339264 2306 50-51 0.32339854027257786 2306 52-53 0.43872728529395744 2306 54-55 0.40331347718996824 2306 56-57 0.32923552358297314 2306 58-59 0.31340240510957074 2306 60-61 0.3961817509570764 2306 62-63 0.37000185189540247 2306 64-65 0.377317901874072 2306 66-67 0.3213337619920509 2306 68-69 0.4221182325918633 2306 70-71 0.5089204384331936 2306 72-73 0.4414756286098793 2306 74-75 0.4558769286535238 2306 76-77 0.06305044009988947 2306 78-79 0.4951741959326341 2306 80-81 0.5518582904469724 2306 82-83 0.6141356968306368 2306 84-85 0.6029133190521705 2306 86-87 0.6358112063211259 2306 88-89 0.5711712657282497 2306 90-91 0.5344675076431038 2306 92-93 0.4975119039646927 2306 94-95 0.5260460025491689 2306 96-97 0.5884959943561476 2306 98-99 0.5044709847132296 2306 100-101 0.37020668303524573 2307 1 0.13638836141435462 2307 2 0.14045370339869123 2307 3 0.10990843106371528 2307 4 0.14709415873520726 2307 5 0.10504017863463844 2307 6 0.13881549272892357 2307 7 0.19350975545171423 2307 8 0.22642425507515895 2307 9 0.1767460197206674 2307 10-11 0.21506232211346088 2307 12-13 0.19634888766817937 2307 14-15 0.32857007068680133 2307 16-17 0.3884340394522994 2307 18-19 0.30424579882953395 2307 20-21 0.3513391900492451 2307 22-23 0.38278137791481726 2307 24-25 0.4102174280093607 2307 26-27 0.4154966708534289 2307 28-29 0.41292689329755206 2307 30-31 0.4481037337967848 2307 32-33 0.4257860974773706 2307 34-35 0.3557024368373263 2307 36-37 0.371089913323857 2307 38-39 0.39437262218086744 2307 40-41 0.3840345581918214 2307 42-43 0.3297601357675859 2307 44-45 0.4024805292917435 2307 46-47 0.2606141069745078 2307 48-49 0.2991273946917232 2307 50-51 0.3210877058036914 2307 52-53 0.36176855243918027 2307 54-55 0.2857705681360443 2307 56-57 0.32250020914575117 2307 58-59 0.2703759256430871 2307 60-61 0.3454655561808764 2307 62-63 0.36769104588297097 2307 64-65 0.31632054826700085 2307 66-67 0.2813637613506401 2307 68-69 0.2841558999392575 2307 70-71 0.22671940431442295 2307 72-73 0.14010245977531355 2307 74-75 0.10932060870081273 2307 76-77 -0.19806957218874288 2307 78-79 0.07646913684821754 2307 80-81 0.317638259826694 2307 82-83 0.24803792012737347 2307 84-85 0.3395241752219462 2307 86-87 0.21020380766692526 2307 88-89 0.2385981179969825 2307 90-91 0.293960750061494 2307 92-93 0.1416772020184034 2307 94-95 0.20524370879995146 2307 96-97 0.2943603536145041 2307 98-99 0.22662637696290844 2307 100-101 0.18364918555463916 2201 1 0.16751053109587843 2201 2 0.12739436655921565 2201 3 0.12382549769763784 2201 4 0.14854696580943028 2201 5 0.09754947263986935 2201 6 0.06080660010525918 2201 7 0.15690778840429687 2201 8 0.09937867055519689 2201 9 0.12358277574219301 2201 10-11 0.18665416046242456 2201 12-13 0.10053349495158415 2201 14-15 0.2860893710724426 2201 16-17 0.2359613241482137 2201 18-19 0.14334147146689702 2201 20-21 0.14821921098194224 2201 22-23 0.19930218541165345 2201 24-25 0.10164127934483247 2201 26-27 0.15586248130907876 2201 28-29 0.2843483069363444 2201 30-31 0.19349782240020375 2201 32-33 0.26126277420419086 2201 34-35 0.30591760446834115 2201 36-37 0.15436885590067817 2201 38-39 0.21522988461845927 2201 40-41 0.20715137890531565 2201 42-43 0.22910246477753304 2201 44-45 0.15561424110768485 2201 46-47 0.20545381707870547 2201 48-49 0.2200845451012725 2201 50-51 0.2686409142798638 2201 52-53 0.1417210430919198 2201 54-55 0.06692247639180948 2201 56-57 0.14755973343762463 2201 58-59 0.15460662491342703 2201 60-61 0.14032081851459566 2201 62-63 0.13583110473119575 2201 64-65 0.09866837022350694 2201 66-67 0.23161493635559793 2201 68-69 0.10841537503889498 2201 70-71 0.23830010713453476 2201 72-73 -0.07739340600223699 2201 74-75 -0.020687225769854933 2201 76-77 -0.07931487027042294 2201 78-79 0.06255009528755551 2201 80-81 0.20454276781230618 2201 82-83 0.2536666397107652 2201 84-85 0.29233619313277615 2201 86-87 0.058253492338653956 2201 88-89 0.12564691030762276 2201 90-91 0.06933073538485601 2201 92-93 0.05709853085002692 2201 94-95 0.05421974878490232 2201 96-97 0.0761980838482792 2201 98-99 -0.02202106579237295 2201 100-101 -0.06225340208866115 1101 1 0.08790941302917332 1101 2 0.09866304090827072 1101 3 0.042072459359722814 1101 4 0.10554595284896351 1101 5 0.09795705964857149 1101 6 0.07991785630095904 1101 7 0.10430981883338575 1101 8 0.05269205132478305 1101 9 0.10223131278493014 1101 10-11 0.13387278703539351 1101 12-13 0.09469814239088237 1101 14-15 0.12487635176714917 1101 16-17 0.12445034937999822 1101 18-19 0.07569289792417067 1101 20-21 0.14536579298694363 1101 22-23 0.1659432334349944 1101 24-25 0.17185577913080863 1101 26-27 0.18171858050849465 1101 28-29 0.18356706989799676 1101 30-31 0.1655737414590206 1101 32-33 0.16311204207050167 1101 34-35 0.17977846445610624 1101 36-37 0.062411508024105444 1101 38-39 0.09490452899243706 1101 40-41 0.1354364435795219 1101 42-43 0.16169657610524268 1101 44-45 0.00816176289011139 1101 46-47 0.10645555294488673 1101 48-49 0.06890920545401258 1101 50-51 0.11720579541061227 1101 52-53 0.09676478643910968 1101 54-55 0.08674658077764974 1101 56-57 0.0477466271044662 1101 58-59 0.0521687804447879 1101 60-61 0.09016113965041228 1101 62-63 -0.026896767036909353 1101 64-65 0.015018293659345261 1101 66-67 0.10674996628615929 1101 68-69 0.05546288646631581 1101 70-71 -0.05620585523569588 1101 72-73 -0.10675732563865381 1101 74-75 -0.10889196301055648 1101 76-77 -0.14712492479270978 1101 78-79 -0.07026366815980012 1101 80-81 0.16366116839429168 1101 82-83 0.29678819416628954 1101 84-85 0.3439888931192385 1101 86-87 0.3090137733496583 1101 88-89 0.24488549310578023 1101 90-91 0.1757638067077778 1101 92-93 0.08363196226419944 1101 94-95 0.10794665496242928 1101 96-97 0.1660208232032403 1101 98-99 0.16776075696208537 1101 100-101 0.11094693398583289 2304 1 0.03197415248686042 2304 2 0.07042457911652633 2304 3 0.027327020242417177 2304 4 0.0888642835672897 2304 5 0.11860846111576251 2304 6 0.10003923762325684 2304 7 0.05410240910015318 2304 8 0.06374398803490777 2304 9 0.03150257585648575 2304 10-11 0.0369983266676428 2304 12-13 0.034010381644961285 2304 14-15 0.08088441137630298 2304 16-17 0.08817326248631474 2304 18-19 0.06066296133357696 2304 20-21 0.056516498202746845 2304 22-23 0.05290952714707231 2304 24-25 0.06842223947668202 2304 26-27 0.06748724544916485 2304 28-29 0.09194530011789226 2304 30-31 0.07214069621512209 2304 32-33 0.1050296477295305 2304 34-35 0.14189648482106065 2304 36-37 0.05386602470921531 2304 38-39 0.10288873800472942 2304 40-41 0.05378443013776035 2304 42-43 0.05703940727571677 2304 44-45 0.11446871360629274 2304 46-47 -0.03077595211311035 2304 48-49 0.011654024442584898 2304 50-51 -0.026435433695262134 2304 52-53 0.019670234044994572 2304 54-55 -0.02684599485291983 2304 56-57 -0.03440850808885898 2304 58-59 -0.07105249104412081 2304 60-61 -0.03948679382323661 2304 62-63 -0.04617685620828382 2304 64-65 -0.12659663798353193 2304 66-67 -0.1157402076977263 2304 68-69 -0.11846896811597674 2304 70-71 -0.08642689169911932 2304 72-73 -0.12176310941251955 2304 74-75 -0.19007897279998787 2304 76-77 -0.4862805319981369 2304 78-79 -0.08795909515424682 2304 80-81 0.03145746436373287 2304 82-83 0.11338782644481427 2304 84-85 0.1865448453383607 2304 86-87 0.054598881011866496 2304 88-89 -0.026679808627406487 2304 90-91 -0.12071700403415875 2304 92-93 -0.12923770098257137 2304 94-95 -0.118409043156678 2304 96-97 -0.07444602390767585 2304 98-99 -0.10668411789793808 2304 100-101 -0.07776980931441457 2202 1 0.058291204082166814 2202 2 0.0697133857034089 2202 3 0.0509633720172431 2202 4 0.055918175381847846 2202 5 0.006819302250498538 2202 6 0.042013202431967045 2202 7 0.052211978496302436 2202 8 0.05329705163101295 2202 9 0.07096053698032989 2202 10-11 0.06274539701346526 2202 12-13 0.037959481042818766 2202 14-15 0.0756924719718981 2202 16-17 0.02500369032909333 2202 18-19 -0.01855320831835172 2202 20-21 -0.01996731666670115 2202 22-23 -0.028555311315592746 2202 24-25 -0.07096920660073636 2202 26-27 0.027545400946074494 2202 28-29 0.027388761679809193 2202 30-31 0.003182374784991282 2202 32-33 -0.024847067151760882 2202 34-35 -0.0634344990246305 2202 36-37 -0.0265369400205131 2202 38-39 0.057959656272394966 2202 40-41 0.040616137369127614 2202 42-43 -0.09809114121687657 2202 44-45 -0.11041039733201075 2202 46-47 -0.06977075764501706 2202 48-49 -0.14898499227705742 2202 50-51 -0.06735560155781428 2202 52-53 -0.047980826731716775 2202 54-55 -0.09316973461426414 2202 56-57 -0.11904785652830441 2202 58-59 -0.1589289276372341 2202 60-61 -0.14139394014198814 2202 62-63 -0.23474091199910418 2202 64-65 -0.22442060761181182 2202 66-67 -0.15755865487943765 2202 68-69 -0.14311843397170065 2202 70-71 -0.13860634662091798 2202 72-73 -0.18020077683875257 2202 74-75 -0.053868090529284984 2202 76-77 -0.41690970409985084 2202 78-79 -0.09034628762650243 2202 80-81 0.1102726429849028 2202 82-83 0.19081390490406847 2202 84-85 0.22586213951928613 2202 86-87 0.1490032639546648 2202 88-89 0.179912223716439 2202 90-91 0.133348279076678 2202 92-93 0.16059109012643802 2202 94-95 0.1490412297535464 2202 96-97 0.17958841663167036 2202 98-99 0.07796019581009972 2202 100-101 -0.032113092944488386 1102 1 0.06543513945494794 1102 2 0.08495807576393588 1102 3 0.06274661447878316 1102 4 0.02938720872416667 1102 5 0.026812406491629304 1102 6 0.027438817042053643 1102 7 -0.02482593607340533 1102 8 5.928275079867262E-4 1102 9 0.03225033159597501 1102 10-11 0.022551963367320127 1102 12-13 -0.010772310317392453 1102 14-15 -0.040860936290030736 1102 16-17 -0.03698886870002838 1102 18-19 -0.03516160083449904 1102 20-21 -0.05725209041538193 1102 22-23 -0.07452770107567375 1102 24-25 0.014662291678646966 1102 26-27 -0.013593028817140862 1102 28-29 -0.10853181932082379 1102 30-31 -0.07337896872388683 1102 32-33 -0.07976754929376995 1102 34-35 -0.1526916247740715 1102 36-37 -0.08191126612590693 1102 38-39 -0.052222222985498945 1102 40-41 -0.08689500899537705 1102 42-43 -0.14269584489527887 1102 44-45 -0.13022771981673742 1102 46-47 -0.1734526700061636 1102 48-49 -0.14310343225572097 1102 50-51 -0.13999848703232942 1102 52-53 -0.17142666566660125 1102 54-55 -0.2143206559393036 1102 56-57 -0.26475106552467054 1102 58-59 -0.24011003463346725 1102 60-61 -0.25176902526433764 1102 62-63 -0.2850342507311012 1102 64-65 -0.2222504166532886 1102 66-67 -0.1539145733467393 1102 68-69 -0.14325123853145527 1102 70-71 -0.27203123696012455 1102 72-73 -0.3722954905107798 1102 74-75 -0.4085301579123879 1102 76-77 -0.5639348470622316 1102 78-79 -0.17906858603982911 1102 80-81 0.005674590259630463 1102 82-83 -0.0672176427927127 1102 84-85 -0.08088215814556321 1102 86-87 -0.14663439481518026 1102 88-89 -0.06465883023848917 1102 90-91 -0.07773025142086354 1102 92-93 -0.08340430432514978 1102 94-95 -0.13535877308606103 1102 96-97 -0.0925745082950371 1102 98-99 -0.16410867281098618 1102 100-101 -0.2272949891413063 2305 1 0.009626138812585339 2305 2 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0.3726705130292203 1213 78-79 -0.1546203562616668 1213 80-81 -0.3184769126755498 1213 82-83 -0.2712602429837929 1213 84-85 -0.2657299538590365 1213 86-87 -0.3097657960268059 1213 88-89 -0.44871693195100093 1213 90-91 -0.2865927534227559 1213 92-93 -0.28352816186799146 1213 94-95 -0.0897017118034178 1213 96-97 -0.2073873413678946 1213 98-99 -0.25049787310478067 1213 100-101 0.037975279636409454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 16.0 8 144.0 9 381.0 10 295.0 11 140.0 12 131.0 13 224.0 14 639.0 15 1447.0 16 2742.0 17 4927.0 18 7549.0 19 11005.0 20 15513.0 21 20954.0 22 28040.0 23 37203.0 24 48627.0 25 63397.0 26 86709.0 27 127706.0 28 209083.0 29 286512.0 30 213635.0 31 182244.0 32 237386.0 33 336101.0 34 517066.0 35 891693.0 36 1712162.0 37 2480856.0 38 589426.0 39 177.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.09688558251965 24.27465537305025 23.33844412923344 14.290014915196664 2 27.409013658888874 27.136131661681535 32.54247836798277 12.912376311446822 3 34.844344651970665 18.253152959176468 31.057219450444904 15.845282938407959 4 26.372877929978777 18.264148571400398 33.54111768722407 21.821855811396752 5 35.844187855013416 17.273373731995914 31.406275226056273 15.476163186934397 6 29.211178524376614 18.418893954126936 36.86302782923123 15.506899692265222 7 30.25206645690912 24.79885089344144 28.443406748474576 16.50567590117486 8 26.327172475668988 31.61513310730787 25.836263407167497 16.221431009855646 9 34.5475238873422 23.316621745029966 24.529789392085167 17.606064975542665 10-11 26.207997653476095 29.45343493387461 27.431813392193614 16.906754020455676 12-13 23.05914353786065 27.847861334137857 27.109710179435265 21.98328494856623 14-15 22.2689062166862 28.47168457986254 27.55594253481273 21.703466668638534 16-17 24.486938217652416 26.208515269043016 28.42154365286235 20.883002860442215 18-19 20.327502763697403 26.455571946715175 31.837584559281158 21.379340730306268 20-21 23.660447885355545 26.717670286278384 31.397642137850884 18.224239690515187 22-23 20.613191798935116 33.15750808128598 28.00729272205466 18.222007397724237 24-25 20.73052088899756 26.053119940922798 31.603962865757108 21.612396304322534 26-27 20.508193731182516 26.170544469955498 28.22275462680534 25.098507172056646 28-29 23.17784531428508 29.77038203726093 27.909880664963467 19.141891983490527 30-31 19.415544241958163 27.07194733138365 31.155046813398357 22.357461613259833 32-33 19.350792999372697 30.14382318252234 28.432949681604807 22.072434136500156 34-35 22.87629111192451 30.342797071281822 28.519490074721503 18.261421742072162 36-37 19.350700568021466 30.859272651535036 31.78918750377428 18.00083927666922 38-39 19.897445870632033 27.276159587993998 30.85738311040846 21.96901143096551 40-41 23.17005027033089 26.877545713465278 30.697419193431706 19.25498482277213 42-43 20.071018088199228 30.24335942362274 31.160358535049355 18.52526395312868 44-45 19.48688692966999 27.415173158684652 31.32270198683332 21.77523792481204 46-47 23.03387805143773 27.187916407650736 28.132605640294777 21.64559990061676 48-49 20.22825242794428 30.5270522115012 30.66758862233092 18.577106738223605 50-51 23.19758592768875 27.481276396983517 30.091630731234186 19.229506944093547 52-53 19.56976896449198 27.326362881496667 27.32831629233995 25.775551861671396 54-55 23.026296451379434 27.25262211179481 30.471889229969086 19.249192206856666 56-57 19.836813531735586 26.996954750652453 30.157680407006076 23.00855131060588 58-59 19.772831208017937 26.759167889553154 30.763643798910195 22.704357103518717 60-61 22.639469983007157 27.0226080736465 30.93229310707759 19.40562883626875 62-63 19.608833188574273 27.123532215540745 30.757004125248066 22.510630470636915 64-65 23.08861604413893 27.18890374384275 30.498584178291 19.22389603372732 66-67 20.18914769651571 33.383026550487536 26.9412954886751 19.486530264321654 68-69 20.156765661822256 33.29345130034018 26.977154096595534 19.572628941242026 70-71 19.72921919325083 34.29833933003277 26.949212853084642 19.02322862363176 72-73 19.717649170022558 34.38165626724729 26.767860210900373 19.132834351829782 74-75 20.524872138763776 33.24676856535413 26.72470080461479 19.503658491267302 76-77 20.81429512866402 31.975340808153685 27.317901546871976 19.89246251631032 78-79 20.6631983530656 31.629624877745982 27.874452248694393 19.832724520494025 80-81 20.090182673092862 31.987710586505962 27.666062092497075 20.2560446479041 82-83 19.32335321223594 31.71852065470975 28.069928630672663 20.888197502381647 84-85 19.439880020946298 31.234638590707046 28.310555857020443 21.014925531326213 86-87 19.340955707827217 31.17351266766223 28.87037810048722 20.615153524023334 88-89 19.567682214734642 31.427263944741572 28.520561534549365 20.48449230597442 90-91 20.049868340850683 30.806971461187004 28.102087559666284 21.04107263829603 92-93 19.665073678108836 31.48442141742272 27.80189333244098 21.04861157202747 94-95 19.412375058398467 31.99082436113262 27.638632464242512 20.958168116226403 96-97 19.870415558267858 32.30058758874765 27.081687746914994 20.74730910606949 98-99 20.18268691366616 31.312201512085075 27.03911882155398 21.465992752694785 100-101 20.205600276230616 31.47298570698836 26.754960137168737 21.566453879612286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 71.5 2 110.0 3 349.5 4 416.0 5 258.0 6 201.0 7 191.0 8 184.0 9 191.0 10 187.5 11 191.0 12 213.5 13 216.5 14 224.0 15 258.5 16 302.0 17 390.0 18 519.5 19 742.5 20 1203.0 21 1830.0 22 2649.5 23 4012.0 24 5970.5 25 8394.0 26 11847.5 27 16646.0 28 22046.5 29 27683.5 30 34345.5 31 42078.5 32 53104.5 33 77935.5 34 120830.0 35 200988.5 36 312114.5 37 431301.0 38 564551.5 39 632495.0 40 635842.0 41 592145.5 42 529764.5 43 520456.0 44 496010.0 45 454230.0 46 442533.5 47 393083.5 48 300403.0 49 242045.5 50 199996.0 51 164081.0 52 142485.5 53 118833.5 54 87901.0 55 58619.5 56 40522.0 57 29508.0 58 22755.5 59 17417.0 60 12918.0 61 9740.5 62 7019.0 63 4995.5 64 3819.0 65 2813.0 66 1964.5 67 1481.5 68 1124.5 69 740.0 70 480.5 71 328.0 72 220.0 73 149.0 74 100.5 75 78.5 76 64.5 77 45.5 78 33.5 79 25.5 80 12.5 81 8.5 82 10.5 83 8.5 84 6.0 85 3.5 86 2.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15190784471040025 2 0.0 3 0.004966644606384171 4 0.007271266297187745 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.394508098834996E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.6021434214142491E-4 24-25 2.4648360329449982E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.058272421072869E-4 40-41 0.0 42-43 0.0 44-45 1.4789016197669992E-4 46-47 0.0010598794941663492 48-49 0.002692833365992411 50-51 4.929672065889997E-4 52-53 0.0014480911693551864 54-55 0.002495646483356811 56-57 0.00424568006674776 58-59 0.005810850947667834 60-61 0.001552846700755349 62-63 8.93503061942562E-4 64-65 0.001404956538778649 66-67 0.008405090872342444 68-69 0.007622505431882408 70-71 0.009588212168156043 72-73 0.006605760568292596 74-75 0.009175352132637756 76-77 0.0049419962460547215 78-79 0.0047879439939956595 80-81 0.001904085835450011 82-83 0.0 84-85 5.792364677420746E-4 86-87 0.001515874160261174 88-89 0.004923509975807635 90-91 0.0069693238831519825 92-93 0.009409511555767532 94-95 0.008497522223577882 96-97 0.01093770989619343 98-99 0.0077827197740238324 100-101 0.0076348296120471336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8114130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.10772864027729 #Duplication Level Percentage of deduplicated Percentage of total 1 89.38030477338155 30.485591809957786 2 4.494463547491527 3.0659188612291803 3 1.5884181101810633 1.625320016080733 4 1.0580660798262684 1.4435292293678528 5 0.5929019810541342 1.0111269940038616 6 0.3766902374443447 0.7708829040115995 7 0.2948169227398651 0.7038874919561039 8 0.23337989302538914 0.6368046449125531 9 0.14782458704083679 0.45377648110349195 >10 1.4025434546349378 9.708808677160434 >50 0.1875733882596283 4.539381540818061 >100 0.18341889108473716 13.636275580368915 >500 0.032196066388259066 7.539093371933775 >1k 0.026633216299491783 16.44921176704407 >5k 5.126755439613229E-4 1.0823244147576223 >10k+ 2.561756040798715E-4 6.848066215293938 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 473902 5.840453628423504 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16073 0.19808654778762483 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13498 0.16635178386345795 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13104 0.1614960568785563 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11181 0.13779665842179012 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10650 0.13125251875432115 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10217 0.12591614874299525 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9598 0.11828748122103047 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8766 0.10803376332397929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018979237453676488 0.0 0.0 2 0.0021197589883326985 0.0 0.0 3 0.0023662425916271984 0.0 0.0 4 0.0025880778345922484 0.0 1.2324180164724993E-5 5 0.002982451599863448 0.0 1.2324180164724993E-5 6 0.0033152044643110228 0.0 1.2324180164724993E-5 7 0.0038204958510647475 0.0 2.4648360329449985E-5 8 0.004325787237818473 0.0 3.6972540494174974E-5 9 0.004954320426219447 0.0 3.6972540494174974E-5 10-11 0.008331145791354095 0.0 3.6972540494174974E-5 12-13 0.011498460093688417 0.0 4.929672065889997E-5 14-15 0.014733557386928728 0.0 4.929672065889997E-5 16-17 0.0175311462843213 0.0 4.929672065889997E-5 18-19 0.020963430460197212 0.0 5.5458810741262465E-5 20-21 0.025147489626121344 0.0 6.162090082362496E-5 22-23 0.029892298989540468 0.0 6.162090082362496E-5 24-25 0.03506845465872496 0.0 6.778299090598745E-5 26-27 0.040355527949391984 0.0 8.010717107071244E-5 28-29 0.04638205204994251 0.0 8.626926115307494E-5 30-31 0.07136316524384007 0.0 8.626926115307494E-5 32-33 0.07911507456745209 0.0 8.626926115307494E-5 34-35 0.08607207427043935 0.0 8.626926115307494E-5 36-37 0.09323858503622692 0.0 9.859344131779994E-5 38-39 0.10077482120695627 0.0 1.1091762148252493E-4 40-41 0.10881634876443932 0.0 1.1091762148252493E-4 42-43 0.11737549188884083 0.0 1.1091762148252493E-4 44-45 0.12684046225534962 0.0 1.478901619766999E-4 46-47 0.13601581438798738 0.0 1.478901619766999E-4 48-49 0.1461709388437208 0.0 1.478901619766999E-4 50-51 0.1569299481275257 0.0 1.478901619766999E-4 52-53 0.16813262789726072 0.0 1.478901619766999E-4 54-55 0.18032740417025608 0.0 1.478901619766999E-4 56-57 0.1936313566580767 0.0 1.5405225205906238E-4 58-59 0.20786578474833406 0.0 1.602143421414249E-4 60-61 0.2243124031781596 0.0 1.602143421414249E-4 62-63 0.24261381072277619 0.0 1.663764322237874E-4 64-65 0.2608905699070634 0.0 1.848627024708749E-4 66-67 0.28109606328713 0.0 1.9718688263559988E-4 68-69 0.30322412877289373 0.0 1.9718688263559988E-4 70-71 0.32753357414781376 0.0 1.9718688263559988E-4 72-73 0.35465293260029107 0.0 1.9718688263559988E-4 74-75 0.38513063014765603 0.0 2.033489727179624E-4 76-77 0.4181779192593661 0.0 2.0951106280032487E-4 78-79 0.45494711078082306 0.0 2.0951106280032487E-4 80-81 0.49609138626075744 0.0 2.0951106280032487E-4 82-83 0.5423132239685586 0.0 2.0951106280032487E-4 84-85 0.5951962810553935 0.0 2.0951106280032487E-4 86-87 0.6533047905320718 0.0 2.1567315288268735E-4 88-89 0.7190419675307149 0.0 2.2183524296504986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 61065 0.0 82.34576 3 CGGAAGA 60865 0.0 82.32505 4 GATCGGA 61450 0.0 81.99648 1 GAAGAGC 61815 0.0 81.07021 6 ATCGGAA 62135 0.0 80.943016 2 GGAAGAG 65070 0.0 77.10243 5 AGAGCAC 89800 0.0 55.83218 8 GAGCACA 90035 0.0 55.770866 9 AAGAGCA 90575 0.0 55.34397 7 TTGTGTT 92955 0.0 46.63158 1 ATGCCGT 54170 0.0 45.552097 48-49 GCCGTCT 54365 0.0 45.52948 50-51 TCGTATG 54720 0.0 45.462177 44-45 TGCCGTC 54295 0.0 45.46133 50-51 TATGCCG 54290 0.0 45.447037 48-49 CGTCTTC 54530 0.0 45.405483 52-53 CGTATGC 54515 0.0 45.34126 46-47 TCTCGTA 54455 0.0 45.277824 42-43 CTCGTAT 54420 0.0 45.276398 44-45 CGTATCT 54055 0.0 45.265232 38-39 >>END_MODULE