FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8114130
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4739025.840453628423504TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC160730.19808654778762483No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA134980.16635178386345795No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG131040.1614960568785563No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA111810.13779665842179012No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC106500.13125251875432115No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC102170.12591614874299525No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA95980.11828748122103047No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG87660.10803376332397929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG610650.082.345763
CGGAAGA608650.082.325054
GATCGGA614500.081.996481
GAAGAGC618150.081.070216
ATCGGAA621350.080.9430162
GGAAGAG650700.077.102435
AGAGCAC898000.055.832188
GAGCACA900350.055.7708669
AAGAGCA905750.055.343977
TTGTGTT929550.046.631581
ATGCCGT541700.045.55209748-49
GCCGTCT543650.045.5294850-51
TCGTATG547200.045.46217744-45
TGCCGTC542950.045.4613350-51
TATGCCG542900.045.44703748-49
CGTCTTC545300.045.40548352-53
CGTATGC545150.045.3412646-47
TCTCGTA544550.045.27782442-43
CTCGTAT544200.045.27639844-45
CGTATCT540550.045.26523238-39
Produced by FastQC (version 0.11.2)