##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5187311 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6629558166071 33.0 33.0 33.0 33.0 33.0 2 32.7009433211157 33.0 33.0 33.0 33.0 33.0 3 32.72759431620737 33.0 33.0 33.0 33.0 33.0 4 36.668385026461685 37.0 37.0 37.0 37.0 37.0 5 36.66362051552336 37.0 37.0 37.0 37.0 37.0 6 36.5363742409121 37.0 37.0 37.0 37.0 37.0 7 36.465069667116545 37.0 37.0 37.0 37.0 37.0 8 36.39528167869634 37.0 37.0 37.0 37.0 37.0 9 36.409319202183944 37.0 37.0 37.0 37.0 37.0 10-11 36.3508854587666 37.0 37.0 37.0 37.0 37.0 12-13 36.34125773449867 37.0 37.0 37.0 37.0 37.0 14-15 38.16127614095241 40.0 37.0 40.0 37.0 40.0 16-17 38.12328661227368 40.0 37.0 40.0 37.0 40.0 18-19 38.12169156620838 40.0 37.0 40.0 37.0 40.0 20-21 38.0756700340504 40.0 37.0 40.0 37.0 40.0 22-23 37.96703156992129 40.0 37.0 40.0 33.0 40.0 24-25 37.91849669318073 40.0 37.0 40.0 33.0 40.0 26-27 37.79615594669377 40.0 37.0 40.0 33.0 40.0 28-29 37.67049585035484 40.0 37.0 40.0 33.0 40.0 30-31 37.61948040131004 40.0 37.0 40.0 33.0 40.0 32-33 37.47136493262116 40.0 37.0 40.0 33.0 40.0 34-35 37.38850302208601 40.0 37.0 40.0 33.0 40.0 36-37 37.29747811534724 40.0 37.0 40.0 33.0 40.0 38-39 37.163306094429274 40.0 37.0 40.0 33.0 40.0 40-41 37.0846049137983 40.0 37.0 40.0 33.0 40.0 42-43 36.92099018161818 37.0 37.0 40.0 33.0 40.0 44-45 36.795851646450345 37.0 37.0 40.0 33.0 40.0 46-47 36.73769155155725 37.0 37.0 40.0 33.0 40.0 48-49 36.49731219122971 37.0 37.0 40.0 33.0 40.0 50-51 36.32671696376022 37.0 37.0 40.0 33.0 40.0 52-53 36.176541757376796 37.0 37.0 40.0 33.0 40.0 54-55 35.98464801898325 37.0 37.0 40.0 33.0 40.0 56-57 35.802463935553504 37.0 37.0 40.0 27.0 40.0 58-59 35.50449635659015 37.0 33.0 40.0 27.0 40.0 60-61 35.22439497072761 37.0 33.0 40.0 27.0 40.0 62-63 34.95064485241004 37.0 33.0 38.5 27.0 40.0 64-65 34.644407574560304 37.0 33.0 37.0 27.0 40.0 66-67 34.26214034593261 37.0 33.0 37.0 27.0 40.0 68-69 33.97473401922499 37.0 33.0 37.0 27.0 40.0 70-71 33.547113909306766 37.0 33.0 37.0 27.0 38.5 72-73 33.09307057163143 37.0 33.0 37.0 24.5 37.0 74-75 32.640580158004795 37.0 33.0 37.0 22.0 37.0 76-77 29.987172062750815 33.0 30.0 33.0 22.0 37.0 78-79 32.39737033310708 33.0 33.0 37.0 24.5 37.0 80-81 32.82406934922545 37.0 33.0 37.0 27.0 37.0 82-83 32.78694481591715 37.0 33.0 37.0 27.0 37.0 84-85 32.62204184403056 37.0 33.0 37.0 22.0 37.0 86-87 32.47085185368681 35.0 33.0 37.0 22.0 37.0 88-89 32.33914864560849 33.0 33.0 37.0 22.0 37.0 90-91 32.168120534897554 33.0 33.0 37.0 22.0 37.0 92-93 32.009534997998 33.0 33.0 37.0 22.0 37.0 94-95 31.827647600076418 33.0 33.0 37.0 22.0 37.0 96-97 31.665450654491316 33.0 33.0 37.0 18.5 37.0 98-99 31.527147495108736 33.0 33.0 37.0 15.0 37.0 100-101 29.933078814823325 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16725421335465285 2306 2 0.17048222116189038 2306 3 0.1383258261972813 2306 4 0.1685324075692307 2306 5 0.18924974228097113 2306 6 0.18124767554451893 2306 7 0.17620948366048594 2306 8 0.24116383886257609 2306 9 0.20922099266015692 2306 10-11 0.22772196114409127 2306 12-13 0.2093180127864045 2306 14-15 0.37980672473657506 2306 16-17 0.36466233256727065 2306 18-19 0.408421956663112 2306 20-21 0.4060597188705941 2306 22-23 0.427171214961497 2306 24-25 0.43357078892319834 2306 26-27 0.5085364349274712 2306 28-29 0.47518572899991085 2306 30-31 0.5499439834044395 2306 32-33 0.5055601087187114 2306 34-35 0.5610368780374557 2306 36-37 0.5644605549732802 2306 38-39 0.5600249329054705 2306 40-41 0.4905675632374269 2306 42-43 0.6047814465978405 2306 44-45 0.6710073136324439 2306 46-47 0.6462331051258872 2306 48-49 0.5709377795917234 2306 50-51 0.5526492285514806 2306 52-53 0.6319238074817477 2306 54-55 0.5627516065974589 2306 56-57 0.58967213542698 2306 58-59 0.5578158379072775 2306 60-61 0.5706470153023773 2306 62-63 0.5177074474573615 2306 64-65 0.5236641179665824 2306 66-67 0.5285345311535394 2306 68-69 0.5453980698651719 2306 70-71 0.5347669119022456 2306 72-73 0.36478948488411334 2306 74-75 0.37976572279184495 2306 76-77 0.06310909578386159 2306 78-79 0.312205637783606 2306 80-81 0.5012727755204835 2306 82-83 0.5329200403690137 2306 84-85 0.552688751572056 2306 86-87 0.4409944477597989 2306 88-89 0.41498264688600983 2306 90-91 0.5303508103188221 2306 92-93 0.47126526985213246 2306 94-95 0.4152142141018693 2306 96-97 0.5633924881949817 2306 98-99 0.5508987834705188 2306 100-101 0.33522352323753424 2307 1 0.15824394708444345 2307 2 0.15153459129083302 2307 3 0.13203895673067478 2307 4 0.1524417742006392 2307 5 0.14627263113174394 2307 6 0.15848682555738236 2307 7 0.20765687527973853 2307 8 0.22013680681294545 2307 9 0.20556722256362292 2307 10-11 0.21115306706131065 2307 12-13 0.24282223222584065 2307 14-15 0.3990694426178507 2307 16-17 0.4111145547418431 2307 18-19 0.3066193435173332 2307 20-21 0.36781428854018827 2307 22-23 0.37799187329888184 2307 24-25 0.46651559312785906 2307 26-27 0.4870412976467833 2307 28-29 0.4249744592130966 2307 30-31 0.42701269403332986 2307 32-33 0.4920235942472928 2307 34-35 0.5053462039278074 2307 36-37 0.48366686674006587 2307 38-39 0.4407239160467711 2307 40-41 0.4508018183669904 2307 42-43 0.35720274011453057 2307 44-45 0.4922779581512202 2307 46-47 0.36729798943081704 2307 48-49 0.45949808946202353 2307 50-51 0.5161371396549654 2307 52-53 0.515046883598437 2307 54-55 0.4632203372394912 2307 56-57 0.4753074874678518 2307 58-59 0.47406273467753834 2307 60-61 0.3904982747014145 2307 62-63 0.44863667118533357 2307 64-65 0.3267745548250858 2307 66-67 0.4894461063791695 2307 68-69 0.524589067997276 2307 70-71 0.4339125111531814 2307 72-73 0.32139385466044246 2307 74-75 0.2785172237782092 2307 76-77 -0.15867888274488706 2307 78-79 0.25108052937903125 2307 80-81 0.30296549502186565 2307 82-83 0.3072575216662443 2307 84-85 0.3980465938684219 2307 86-87 0.35663368330506273 2307 88-89 0.41822048889647334 2307 90-91 0.39040592987358025 2307 92-93 0.44235296676724545 2307 94-95 0.4532185633885497 2307 96-97 0.4866897323363695 2307 98-99 0.3607158894735072 2307 100-101 0.29945433324601467 2201 1 0.1498136588354484 2201 2 0.10641490580574242 2201 3 0.12792320376557598 2201 4 0.11893706583906294 2201 5 0.08610822856444145 2201 6 0.09392927414812391 2201 7 0.1396086868481916 2201 8 0.12761238730715974 2201 9 0.10615076163222881 2201 10-11 0.13225374247692656 2201 12-13 0.11352525089979082 2201 14-15 0.32603289614450404 2201 16-17 0.26436869552545517 2201 18-19 0.23499789526813686 2201 20-21 0.23798971325928164 2201 22-23 0.27564460573495353 2201 24-25 0.226647748424476 2201 26-27 0.2611044689231292 2201 28-29 0.35344473581146474 2201 30-31 0.4015752483205759 2201 32-33 0.46526789506231836 2201 34-35 0.39090650784099523 2201 36-37 0.29734991681174705 2201 38-39 0.41680740700453356 2201 40-41 0.3206569104450807 2201 42-43 0.39474626742848784 2201 44-45 0.37552766818583905 2201 46-47 0.41903955334542786 2201 48-49 0.3702442052696 2201 50-51 0.46425500863049507 2201 52-53 0.2970994353804457 2201 54-55 0.308268381988654 2201 56-57 0.2515576164135922 2201 58-59 0.3836145853848123 2201 60-61 0.3224271686026867 2201 62-63 0.23905289828066856 2201 64-65 0.21653857111862607 2201 66-67 0.3709374949709101 2201 68-69 0.28078683413695416 2201 70-71 0.3788825599835235 2201 72-73 0.2518427169472659 2201 74-75 0.26217378471081076 2201 76-77 -0.00916432011149837 2201 78-79 0.22248908712852256 2201 80-81 0.3976395700366453 2201 82-83 0.4003737371022922 2201 84-85 0.3131243925760998 2201 86-87 0.25367703876360537 2201 88-89 0.3104344200641549 2201 90-91 0.2636819036631408 2201 92-93 0.21121743049166497 2201 94-95 0.3337797818412618 2201 96-97 0.4011202830278684 2201 98-99 0.3370901584920283 2201 100-101 0.2534484708742468 1101 1 0.05749369173555152 1101 2 0.0743503503490004 1101 3 0.024609312068811562 1101 4 0.08169333317279381 1101 5 0.10619461532699148 1101 6 0.11234931346191246 1101 7 0.08421416750638144 1101 8 0.07983771262952644 1101 9 0.10468089313667406 1101 10-11 0.08695607211452483 1101 12-13 0.05898447284513253 1101 14-15 0.1477129016545078 1101 16-17 0.08906501033801106 1101 18-19 0.03015881507953111 1101 20-21 0.10090078461587382 1101 22-23 0.17365228615771144 1101 24-25 0.14733200399766133 1101 26-27 0.1817801026139918 1101 28-29 0.191012721227942 1101 30-31 0.1811503539623729 1101 32-33 0.18638021584712305 1101 34-35 0.21306916382459207 1101 36-37 0.12032387942686995 1101 38-39 0.1484947538599073 1101 40-41 0.11059394421366875 1101 42-43 0.18530590106496447 1101 44-45 0.027153240378936516 1101 46-47 0.17066086707838224 1101 48-49 0.1550356996652269 1101 50-51 0.17238209987429798 1101 52-53 0.24070390300494893 1101 54-55 0.15532333691695044 1101 56-57 0.13869717674857895 1101 58-59 0.1322765692114629 1101 60-61 0.041338910116955674 1101 62-63 0.12733889406459298 1101 64-65 0.11159271953855665 1101 66-67 0.0596670503636787 1101 68-69 -0.015708178266564232 1101 70-71 -0.07874153814259444 1101 72-73 -0.06593764867253071 1101 74-75 0.03777950068391789 1101 76-77 -0.04374836452103992 1101 78-79 0.0430325036291066 1101 80-81 0.14191846378867012 1101 82-83 0.2502475227413967 1101 84-85 0.301171089890353 1101 86-87 0.24297150988284955 1101 88-89 0.1601744790588171 1101 90-91 0.0881089658374492 1101 92-93 0.09901202896485728 1101 94-95 0.2198162410576181 1101 96-97 0.12287804542098613 1101 98-99 0.052101560369109734 1101 100-101 0.161539055318908 2304 1 0.06451001103179976 2304 2 0.06782455839186952 2304 3 0.06224431823375198 2304 4 0.11719506409706071 2304 5 0.09965103990137436 2304 6 0.09056604652019473 2304 7 0.06235606180144515 2304 8 0.08040456068756896 2304 9 0.06409605413634267 2304 10-11 0.005873088522740488 2304 12-13 0.08039725086002392 2304 14-15 0.1195101730776642 2304 16-17 0.11654232159613542 2304 18-19 0.0505809057040878 2304 20-21 0.07024653081020915 2304 22-23 0.1180996945135675 2304 24-25 0.09493560752115826 2304 26-27 0.12278566883962583 2304 28-29 0.10760306717568113 2304 30-31 0.006257351164443037 2304 32-33 0.0923734670208276 2304 34-35 0.17954806670314127 2304 36-37 0.11510369044787438 2304 38-39 0.15352784593084579 2304 40-41 0.10395589411171358 2304 42-43 0.02379792221543653 2304 44-45 0.16249877317164874 2304 46-47 0.08736161872631953 2304 48-49 0.14122350198419298 2304 50-51 0.043744639590713064 2304 52-53 0.16697327216603952 2304 54-55 0.11896874048312611 2304 56-57 0.028175143031120342 2304 58-59 -0.07260825319097108 2304 60-61 0.04068092178290783 2304 62-63 0.04250433959975197 2304 64-65 -0.036149372745789776 2304 66-67 -0.07944413188129573 2304 68-69 -0.06032134336828676 2304 70-71 -0.0759254518097805 2304 72-73 0.016898118032074194 2304 74-75 0.1109951519145369 2304 76-77 -0.32712422998317336 2304 78-79 0.08004768181946531 2304 80-81 0.13029733762088824 2304 82-83 0.2923750776330394 2304 84-85 0.3548056603603058 2304 86-87 0.24704985198935958 2304 88-89 0.31272292419090064 2304 90-91 0.15958628302340117 2304 92-93 0.16427921075933227 2304 94-95 0.21677452696836141 2304 96-97 0.21787362919297593 2304 98-99 0.23263562756691059 2304 100-101 0.2410526478166588 2202 1 0.01653950399466453 2202 2 0.029886856517236993 2202 3 0.03134713857004101 2202 4 -0.016196498185649943 2202 5 0.016488582845219923 2202 6 0.02891875810535538 2202 7 0.032283592448713705 2202 8 0.036200053766890505 2202 9 -0.003142240988523781 2202 10-11 0.02879018334345318 2202 12-13 0.10941834515324445 2202 14-15 0.012275675679127573 2202 16-17 -0.001520833693625434 2202 18-19 -0.04806719791604053 2202 20-21 -0.042006724016800945 2202 22-23 -0.008742438241576167 2202 24-25 -0.07808707008569371 2202 26-27 -0.055444753388762535 2202 28-29 0.048291701569802115 2202 30-31 -0.0490011297107813 2202 32-33 -0.11209838784522219 2202 34-35 -0.0753042672501607 2202 36-37 -0.05147374891694767 2202 38-39 -0.039246069897487246 2202 40-41 -0.07477446218095452 2202 42-43 -0.12809721600980595 2202 44-45 -0.04997662937877578 2202 46-47 -0.049354302781061676 2202 48-49 -0.12739670311872686 2202 50-51 -0.0020386425353962068 2202 52-53 0.047680180174580755 2202 54-55 0.02998013451842496 2202 56-57 -0.07660768011386665 2202 58-59 -0.1260185857467775 2202 60-61 -0.16435551840739038 2202 62-63 -0.2160429637148482 2202 64-65 -0.172885845647059 2202 66-67 -0.1875978974104484 2202 68-69 -0.09234137840841328 2202 70-71 -0.12362500156299916 2202 72-73 -0.18152152259254706 2202 74-75 -0.16931699806954015 2202 76-77 -0.5123485912629775 2202 78-79 -0.16285071979587684 2202 80-81 -5.564486309168615E-4 2202 82-83 0.009033951896192605 2202 84-85 0.020375760308589008 2202 86-87 0.005534195252444363 2202 88-89 0.06505522909822758 2202 90-91 0.023295539884998107 2202 92-93 -0.05526743405435042 2202 94-95 -0.022524726159730335 2202 96-97 -0.014030697217958021 2202 98-99 -0.029513671459294955 2202 100-101 -0.00420227081695046 1102 1 0.05506194784542373 1102 2 0.061344256934930286 1102 3 0.05070171148086189 1102 4 -0.0170956624833849 1102 5 0.004326690488234419 1102 6 -0.06760247671842734 1102 7 -0.023744429088765173 1102 8 -0.029036815693061158 1102 9 -0.01538843247431032 1102 10-11 0.006605687094129564 1102 12-13 0.02669925060828149 1102 14-15 -0.04458305672817886 1102 16-17 -0.043211367947272095 1102 18-19 -0.0261826409776873 1102 20-21 -0.09551446701854616 1102 22-23 -0.09625963082705624 1102 24-25 -0.10758118816152518 1102 26-27 -0.08048830925911687 1102 28-29 -0.21078033853249423 1102 30-31 -0.14512701429703156 1102 32-33 -0.004589149801603298 1102 34-35 -0.12064329394382867 1102 36-37 -0.05757323456549557 1102 38-39 -0.054275459711242036 1102 40-41 -0.09056721863570516 1102 42-43 -0.1799136483185606 1102 44-45 -0.18664688905812454 1102 46-47 -0.22869222186023563 1102 48-49 -0.2014526756803079 1102 50-51 -0.24400827900333155 1102 52-53 -0.17752211000456697 1102 54-55 -0.3249368260859882 1102 56-57 -0.31392872272929395 1102 58-59 -0.19664849989477062 1102 60-61 -0.2609569522848716 1102 62-63 -0.34296051140248096 1102 64-65 -0.2552612469394333 1102 66-67 -0.1598480542828824 1102 68-69 -0.2240349986123178 1102 70-71 -0.2663892801240735 1102 72-73 -0.3392195225481771 1102 74-75 -0.4638744553733005 1102 76-77 -0.5919492716773718 1102 78-79 -0.34131843259571326 1102 80-81 -0.11270610018395644 1102 82-83 -0.08632036589884962 1102 84-85 -0.18637916295067924 1102 86-87 -0.14365725805186003 1102 88-89 -0.2037846358330242 1102 90-91 -0.0535468001447299 1102 92-93 -0.09731649709805623 1102 94-95 -0.14571524615225329 1102 96-97 -0.004158805748335936 1102 98-99 -0.12489607575918527 1102 100-101 -0.19333307073962303 2305 1 -0.003814193860314674 2305 2 0.02352111561971526 2305 3 0.019363581694555876 2305 4 -0.008671425284745737 2305 5 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1213 90-91 -0.3125214200554183 1213 92-93 -0.1755808016617877 1213 94-95 -0.10457822596479005 1213 96-97 -0.17079181354380069 1213 98-99 -0.21703599749906033 1213 100-101 0.07755374602817966 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 7.0 8 97.0 9 232.0 10 177.0 11 93.0 12 122.0 13 293.0 14 856.0 15 2231.0 16 4543.0 17 7182.0 18 9261.0 19 10912.0 20 13420.0 21 16144.0 22 20021.0 23 25084.0 24 31097.0 25 39120.0 26 49568.0 27 65106.0 28 91645.0 29 125304.0 30 125030.0 31 131693.0 32 171951.0 33 240689.0 34 366553.0 35 618705.0 36 1149806.0 37 1555009.0 38 315308.0 39 51.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.507757225065234 24.971343012126006 24.28009997364542 14.240799789163344 2 30.835475258761235 23.2088263071175 32.82411638708379 13.131582047037474 3 36.73354304859831 18.035936921935914 28.770859950187322 16.459660079278464 4 29.843539585516304 17.643521655852638 33.870792674286456 18.642146084344606 5 31.388401428023112 16.57109049370666 35.63090780560486 16.409600272665354 6 26.80967075234163 17.518575616538126 39.48488918439631 16.186864446723938 7 33.06682402501026 20.135981821795532 29.29479261991425 17.50240153327996 8 29.71026799819791 28.59932631762391 24.556769393622243 17.133636290555934 9 30.415951540210333 25.194556486009805 25.439461794367062 18.950030179412803 10-11 24.944176279386372 27.288358072226632 29.434065164012722 18.333400484374273 12-13 23.952934739946038 26.172525325274687 28.83807940457748 21.036460530201794 14-15 23.18390009775778 26.895408815858545 29.601011005509402 20.31968008087427 16-17 23.144876631626026 26.780036280846915 30.628476030929324 19.446611056597735 18-19 20.918709134655703 27.026767818625103 31.895118684806057 20.15940436191314 20-21 22.404286151341225 27.304445405336214 31.145722706812833 19.145545736509725 22-23 21.04573214195005 29.84616188352966 30.119367413146346 18.98873856137394 24-25 21.521956613539253 26.71377136837507 31.554729865260594 20.209542152825083 26-27 21.24326071831822 26.729696754252828 30.272312571966474 21.754729955462473 28-29 21.963817091360053 28.384195588041667 29.812797034918475 19.839190285679805 30-31 20.186605352946835 27.75727154203787 30.785555367704 21.270567737311296 32-33 19.873803594964713 29.27469550215902 30.195027828483774 20.656473074392494 34-35 21.521902195569147 28.039691470204893 30.343013943062214 20.095392391163745 36-37 19.907713264155554 28.849041439774865 31.72335339061028 19.519891905459303 38-39 20.436304063768613 29.856326680744765 29.547907235697345 20.159462019789277 40-41 20.496823884282243 27.849824311671306 32.19880203828149 19.45454976576496 42-43 20.365946826785592 29.378631819067717 31.382685557121988 18.8727357970247 44-45 20.029630043746245 28.48354226498262 31.5249689024305 19.961858788840637 46-47 21.64331695825488 28.00656031299806 30.325244433348868 20.024878295398192 48-49 20.880808798198164 30.142160834662096 30.188747083419365 18.788283283720375 50-51 21.576945305846106 29.213844552889164 29.821985772124975 19.387224369139755 52-53 19.950098559441706 28.776195364573542 29.155955255459325 22.117750820525426 54-55 21.716482564853333 28.671388191548143 30.11469075792061 19.49743848567792 56-57 20.56977253054229 28.407501730263906 29.610994260729086 21.41173147846472 58-59 20.36511113561684 28.300291729548277 30.299049777559112 21.03554735727577 60-61 21.21360007996577 28.37880679620363 30.50195774441394 19.905635379416662 62-63 20.1767562657405 28.377093785230574 30.04536502536216 21.40078492366677 64-65 21.655726174391585 28.157830332710425 29.899097369626187 20.287346123271803 66-67 20.90735724687675 30.87192062335142 28.239348022423176 19.98137410734865 68-69 21.429400438681302 30.81391055829204 28.047638776774374 19.709050226252284 70-71 20.73909768021597 31.646377172041127 28.23153400532795 19.382991142414948 72-73 20.26451375147811 31.712509746813712 28.29947417090752 19.723502330800663 74-75 21.327903683743603 30.855776153814197 27.81712797769813 19.99919218474407 76-77 21.73551272511981 29.576964218634373 28.439235182816567 20.248287873429245 78-79 21.50616314773683 29.478174550769893 29.144757097543668 19.87090520394961 80-81 20.499514814706643 30.124179867205648 29.04469439977514 20.33161091831257 82-83 19.55427388101465 29.950296020423682 29.20383990857691 21.291590189984753 84-85 19.38312564581052 29.36254645980167 29.910328727194212 21.343999167193598 86-87 19.670941116311393 29.39507549085806 30.484898695688006 20.449084697142546 88-89 19.758087487460337 29.974857531958733 30.058354084569032 20.208700896011898 90-91 20.209645804587254 29.6723173339806 29.393202811908914 20.724834049523235 92-93 19.544408141916033 30.39230155470213 29.27307874092639 20.790211562455447 94-95 19.431782942074836 30.765460156296463 29.326201998178668 20.476554903450026 96-97 20.1446207198183 31.135986297364653 28.505981455209 20.213411527608045 98-99 20.4080637206987 30.421875475959947 28.285024964882588 20.88503583845876 100-101 20.168418385996734 30.388715503506525 28.38754100712101 21.055325103375733 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4724.0 1 2835.5 2 1096.5 3 1771.0 4 1860.0 5 1222.5 6 1065.0 7 1159.0 8 1228.5 9 1207.5 10 1150.0 11 1068.5 12 992.5 13 966.5 14 964.0 15 991.0 16 1027.5 17 1083.5 18 1169.0 19 1314.5 20 1511.0 21 1778.5 22 2210.0 23 2894.5 24 3770.5 25 4768.5 26 6178.5 27 8165.0 28 10436.0 29 12934.5 30 16143.0 31 21131.0 32 32586.0 33 53536.5 34 82361.0 35 123183.5 36 177430.0 37 265472.0 38 371281.5 39 430255.5 40 428178.0 41 375199.5 42 327998.0 43 320468.0 44 311677.5 45 299356.5 46 294847.5 47 262692.0 48 205500.5 49 163644.0 50 135477.5 51 112317.5 52 90499.5 53 64867.5 54 42603.0 55 29436.0 56 20138.5 57 13890.5 58 9879.0 59 6806.0 60 4840.5 61 3675.0 62 2628.5 63 1708.0 64 1216.0 65 882.5 66 642.0 67 454.5 68 340.5 69 251.5 70 164.0 71 120.5 72 105.0 73 81.0 74 52.5 75 34.5 76 23.5 77 14.0 78 8.0 79 8.5 80 8.5 81 5.0 82 1.5 83 1.5 84 1.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.153181484588065 2 0.0 3 0.005359231401394673 4 0.007248456859440277 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.783343238915114E-5 14-15 0.0 16-17 1.9277810796383715E-5 18-19 0.0 20-21 0.0 22-23 1.4458358097287787E-4 24-25 2.891671619457557E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010988352153938718 40-41 0.0 42-43 0.0 44-45 1.6386139176926157E-4 46-47 0.0011277519315884474 48-49 0.0030458941058286266 50-51 5.590565130951277E-4 52-53 0.0016578917284889995 54-55 0.0027952825654756386 56-57 0.004645952401928475 58-59 0.006072510400860869 60-61 0.00176391968786911 62-63 0.0010602795938011043 64-65 0.0015904193907016564 66-67 0.008588264709788945 68-69 0.007749679940146252 70-71 0.009899155843943037 72-73 0.006930372981299945 74-75 0.009282265898458758 76-77 0.005156814388032643 78-79 0.005205008915023603 80-81 0.0022651427685750863 82-83 0.0 84-85 5.976121346878951E-4 86-87 0.0018024753094618773 88-89 0.005214647820421795 90-91 0.007171345616254742 92-93 0.009590710871200897 94-95 0.008434042223417875 96-97 0.011017268870133294 98-99 0.00819306958846308 100-101 0.007846068994128172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5187311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.70020715800663 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9560284039799 27.780797281316378 2 6.675039969051369 4.3655037955190785 3 2.2167720626664567 2.174667170138244 4 1.2520088095765705 1.6376378974721253 5 0.7846118966323976 1.2828485779257943 6 0.6908240611312837 1.3554053945237046 7 0.34759715506285777 0.7956549284662442 8 0.29272740418490706 0.7657797406137596 9 0.22919537767919704 0.674526269679058 >10 1.9773523829117936 12.874693230545834 >50 0.2654453228148235 6.1004148216242156 >100 0.24946311113660438 17.086819980168883 >500 0.044628792080935314 10.072852026315886 >1k 0.017833207095158548 9.437526485350956 >5k 2.3602388250337073E-4 0.5375973516573714 >10k+ 2.3602011351045148E-4 3.05727504868245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 118584 2.2860399154783666 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15247 0.2939287812124625 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 12697 0.244770363681684 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11923 0.22984933812528302 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8672 0.16717717522623957 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8029 0.15478154288416482 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5988 0.11543553104874568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014265579989323949 0.0 0.0 2 0.0016000582960998483 0.0 0.0 3 0.001831392025656453 0.0 0.0 4 0.0019856145120275225 0.0 0.0 5 0.0021976704307877432 0.0 0.0 6 0.002641060079104569 0.0 0.0 7 0.003026616295032243 0.0 0.0 8 0.0034314503217563012 0.0 0.0 9 0.0038941177808695104 0.0 0.0 10-11 0.00700748422448548 0.0 0.0 12-13 0.010496767978630932 0.0 0.0 14-15 0.015316220677726861 0.0 0.0 16-17 0.02149475903796784 0.0 0.0 18-19 0.029870967828996564 0.0 0.0 20-21 0.03614589524321946 0.0 0.0 22-23 0.042430461562840555 0.0 0.0 24-25 0.04944758469272423 0.0 0.0 26-27 0.057602098659594536 0.0 0.0 28-29 0.06630603023416179 0.0 0.0 30-31 0.09557938592847046 0.0 0.0 32-33 0.10653882136621459 0.0 0.0 34-35 0.11702595043944733 0.0 0.0 36-37 0.12796610806639508 0.0 0.0 38-39 0.13963882250360543 0.0 0.0 40-41 0.151976621413291 0.0 0.0 42-43 0.16534578320058313 0.0 0.0 44-45 0.17963064100070344 0.0 0.0 46-47 0.1948119170028556 0.0 0.0 48-49 0.21056188842350113 0.0 0.0 50-51 0.22627330422255384 0.0 0.0 52-53 0.24273655464266553 0.0 0.0 54-55 0.26014441779180003 0.0 0.0 56-57 0.2789113666020796 0.0 0.0 58-59 0.2988831785871331 0.0 0.0 60-61 0.3200694926523588 0.0 0.0 62-63 0.3419016133792634 0.0 0.0 64-65 0.36559404284801894 0.0 9.638905398191858E-6 66-67 0.39095400295066174 0.0 1.9277810796383715E-5 68-69 0.418752606119047 0.0 1.9277810796383715E-5 70-71 0.4473512384354823 0.0 1.9277810796383715E-5 72-73 0.4773571509400535 0.0 1.9277810796383715E-5 74-75 0.5099173733751456 0.0 1.9277810796383715E-5 76-77 0.5445403215654507 0.0 1.9277810796383715E-5 78-79 0.5814669681459237 0.0 1.9277810796383715E-5 80-81 0.6214009532106326 0.0 1.9277810796383715E-5 82-83 0.6635615254223238 0.0 1.9277810796383715E-5 84-85 0.7099342992930249 0.0 1.9277810796383715E-5 86-87 0.760683136214505 0.0 1.9277810796383715E-5 88-89 0.8164634817538412 0.0 1.9277810796383715E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 15 6.57951E-4 95.14403 1 CGGAAGA 18395 0.0 69.63924 4 TCGGAAG 18545 0.0 69.25858 3 GATCGGA 18765 0.0 68.42359 1 GAAGAGC 18810 0.0 68.02509 6 ATCGGAA 19110 0.0 67.1612 2 GGAAGAG 20075 0.0 63.99883 5 CGGACGG 75 2.3574103E-9 50.74348 1 CGACGCC 90 2.237357E-10 47.572018 1 CGTCGCC 40 5.389932E-4 47.50002 2 GCCGTCT 14665 0.0 43.3217 50-51 CGTCTTC 14705 0.0 43.236576 52-53 ATGCCGT 14755 0.0 42.992447 48-49 TCGTATG 14880 0.0 42.981804 44-45 TATGCCG 14730 0.0 42.710705 48-49 TGCCGTC 14760 0.0 42.672783 50-51 CGTATGC 14810 0.0 42.575592 46-47 CTGCTTG 15050 0.0 42.38747 58-59 CTCGTAT 14810 0.0 42.367123 44-45 TCTCGTA 14930 0.0 42.344738 42-43 >>END_MODULE