FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5187311
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1185842.2860399154783666TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152470.2939287812124625No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC126970.244770363681684No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG119230.22984933812528302No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA86720.16717717522623957No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA80290.15478154288416482No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA59880.11543553104874568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG156.57951E-495.144031
CGGAAGA183950.069.639244
TCGGAAG185450.069.258583
GATCGGA187650.068.423591
GAAGAGC188100.068.025096
ATCGGAA191100.067.16122
GGAAGAG200750.063.998835
CGGACGG752.3574103E-950.743481
CGACGCC902.237357E-1047.5720181
CGTCGCC405.389932E-447.500022
GCCGTCT146650.043.321750-51
CGTCTTC147050.043.23657652-53
ATGCCGT147550.042.99244748-49
TCGTATG148800.042.98180444-45
TATGCCG147300.042.71070548-49
TGCCGTC147600.042.67278350-51
CGTATGC148100.042.57559246-47
CTGCTTG150500.042.3874758-59
CTCGTAT148100.042.36712344-45
TCTCGTA149300.042.34473842-43
Produced by FastQC (version 0.11.2)