##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4478203 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68469607116962 33.0 33.0 33.0 33.0 33.0 2 32.7240893277951 33.0 33.0 33.0 33.0 33.0 3 32.75036638580252 33.0 33.0 33.0 33.0 33.0 4 36.696972870591175 37.0 37.0 37.0 37.0 37.0 5 36.69513329342149 37.0 37.0 37.0 37.0 37.0 6 36.57557439892743 37.0 37.0 37.0 37.0 37.0 7 36.52123519188389 37.0 37.0 37.0 37.0 37.0 8 36.46933379304154 37.0 37.0 37.0 37.0 37.0 9 36.47190937972218 37.0 37.0 37.0 37.0 37.0 10-11 36.42867205885932 37.0 37.0 37.0 37.0 37.0 12-13 36.42154509297591 37.0 37.0 37.0 37.0 37.0 14-15 38.33734502433231 40.0 37.0 40.0 37.0 40.0 16-17 38.30507035969562 40.0 37.0 40.0 37.0 40.0 18-19 38.301387409190696 40.0 37.0 40.0 37.0 40.0 20-21 38.24018685620102 40.0 37.0 40.0 37.0 40.0 22-23 38.148479870162205 40.0 37.0 40.0 37.0 40.0 24-25 38.110679551596924 40.0 37.0 40.0 37.0 40.0 26-27 38.029375399016075 40.0 37.0 40.0 37.0 40.0 28-29 37.9051162932989 40.0 37.0 40.0 33.0 40.0 30-31 37.86523857895678 40.0 37.0 40.0 33.0 40.0 32-33 37.73019925626417 40.0 37.0 40.0 33.0 40.0 34-35 37.64642402320752 40.0 37.0 40.0 33.0 40.0 36-37 37.54752140534942 40.0 37.0 40.0 33.0 40.0 38-39 37.4689714825344 40.0 37.0 40.0 33.0 40.0 40-41 37.409639647867685 40.0 37.0 40.0 33.0 40.0 42-43 37.271783235373654 40.0 37.0 40.0 33.0 40.0 44-45 37.145788612083905 40.0 37.0 40.0 33.0 40.0 46-47 37.08882804106915 40.0 37.0 40.0 33.0 40.0 48-49 36.90641112071069 38.5 37.0 40.0 33.0 40.0 50-51 36.76027359635104 37.0 37.0 40.0 33.0 40.0 52-53 36.60971621876007 37.0 37.0 40.0 33.0 40.0 54-55 36.43858596852353 37.0 37.0 40.0 33.0 40.0 56-57 36.267221137585764 37.0 37.0 40.0 33.0 40.0 58-59 36.062415772576635 37.0 37.0 40.0 33.0 40.0 60-61 35.78002705549525 37.0 37.0 40.0 27.0 40.0 62-63 35.516469552630824 37.0 35.0 40.0 27.0 40.0 64-65 35.24900992652633 37.0 33.0 38.5 27.0 40.0 66-67 34.96421142587775 37.0 33.0 37.0 27.0 40.0 68-69 34.6958564629607 37.0 33.0 37.0 27.0 40.0 70-71 34.33188848294729 37.0 33.0 37.0 27.0 40.0 72-73 34.029499779264135 37.0 33.0 37.0 27.0 38.5 74-75 33.67426621794501 37.0 33.0 37.0 27.0 37.0 76-77 30.951041857637986 33.0 30.0 35.0 24.5 37.0 78-79 33.39770416839076 35.0 33.0 37.0 27.0 37.0 80-81 33.78772378116847 37.0 33.0 37.0 27.0 37.0 82-83 33.726367473738904 37.0 33.0 37.0 27.0 37.0 84-85 33.54751247319516 37.0 33.0 37.0 27.0 37.0 86-87 33.380177718607214 37.0 33.0 37.0 27.0 37.0 88-89 33.229720716099735 37.0 33.0 37.0 27.0 37.0 90-91 33.07226537073018 37.0 33.0 37.0 27.0 37.0 92-93 32.94875198824171 35.0 33.0 37.0 27.0 37.0 94-95 32.767189428438144 33.0 33.0 37.0 27.0 37.0 96-97 32.611891979885684 33.0 33.0 37.0 27.0 37.0 98-99 32.47583986255201 33.0 33.0 37.0 27.0 37.0 100-101 30.871918937127234 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1673071671723534 2306 2 0.13033146158684872 2306 3 0.12562024909435365 2306 4 0.15841707435266272 2306 5 0.14660835185507892 2306 6 0.12118196674767034 2306 7 0.18208194263110755 2306 8 0.21482817057078307 2306 9 0.16210720245687327 2306 10-11 0.22348981589113492 2306 12-13 0.21511184014000406 2306 14-15 0.4434293017036808 2306 16-17 0.3528537415782509 2306 18-19 0.3736893394862548 2306 20-21 0.3633909018251984 2306 22-23 0.3187922455110126 2306 24-25 0.3698201665561882 2306 26-27 0.41059367732255936 2306 28-29 0.3738027041795817 2306 30-31 0.45493537291132213 2306 32-33 0.48594017948706636 2306 34-35 0.48586023126538436 2306 36-37 0.48500970222620765 2306 38-39 0.45967092085174244 2306 40-41 0.41388516669717035 2306 42-43 0.4067402123154693 2306 44-45 0.5443314377658766 2306 46-47 0.45070203691754074 2306 48-49 0.40547950414330103 2306 50-51 0.36102217850231 2306 52-53 0.5009867380052881 2306 54-55 0.5310502830930943 2306 56-57 0.5600703201105333 2306 58-59 0.4050386094540386 2306 60-61 0.4818343009976829 2306 62-63 0.5049807918176015 2306 64-65 0.5340952310348044 2306 66-67 0.5163960259558777 2306 68-69 0.2716826417266205 2306 70-71 0.3428281281947889 2306 72-73 0.3603019395563294 2306 74-75 0.4134793863078272 2306 76-77 -0.005402935792510277 2306 78-79 0.21303756282051722 2306 80-81 0.40802493102609816 2306 82-83 0.5334267972096214 2306 84-85 0.44832678627691536 2306 86-87 0.40524440042563725 2306 88-89 0.47601563038166717 2306 90-91 0.5816563011821572 2306 92-93 0.5019063175767613 2306 94-95 0.4776880213246315 2306 96-97 0.4839603611030441 2306 98-99 0.5401664554945782 2306 100-101 0.2892490195164079 2307 1 0.14339855988196604 2307 2 0.11280823479306434 2307 3 0.07415531472431525 2307 4 0.11626928347051546 2307 5 0.12383009804599254 2307 6 0.124971287660955 2307 7 0.15914852392394607 2307 8 0.15995615647194228 2307 9 0.11559199325670022 2307 10-11 0.16230158485048207 2307 12-13 0.10460777287865142 2307 14-15 0.2942796451010281 2307 16-17 0.3050219108616332 2307 18-19 0.2104534112002696 2307 20-21 0.26220649202710433 2307 22-23 0.3340309118084974 2307 24-25 0.2930043046110882 2307 26-27 0.2710726632948166 2307 28-29 0.2841379726113473 2307 30-31 0.3503321320112178 2307 32-33 0.33823260931090005 2307 34-35 0.31740885645995576 2307 36-37 0.31785407709524094 2307 38-39 0.3676784819542078 2307 40-41 0.2976536204456295 2307 42-43 0.17181962119683192 2307 44-45 0.2623857681644495 2307 46-47 0.14214354103719273 2307 48-49 0.13670858552968923 2307 50-51 0.11016684223297091 2307 52-53 0.17860219612409622 2307 54-55 0.06355908317615899 2307 56-57 0.21898121687244299 2307 58-59 0.0976641959703386 2307 60-61 0.1747243811081418 2307 62-63 0.20054194152050542 2307 64-65 -0.006004429964384883 2307 66-67 0.08193208969251486 2307 68-69 0.20685019876930255 2307 70-71 0.12371260786969884 2307 72-73 0.0598271606926275 2307 74-75 0.10768515250203592 2307 76-77 -0.2741079064012162 2307 78-79 -0.19623569718113032 2307 80-81 0.12955912950994986 2307 82-83 0.18907116300496085 2307 84-85 0.12326170354725008 2307 86-87 0.020413295137068133 2307 88-89 -0.006460616643664707 2307 90-91 0.1617826009658856 2307 92-93 0.1277787839573108 2307 94-95 -0.03409176565863703 2307 96-97 0.014766205967141843 2307 98-99 0.021808121163452654 2307 100-101 0.021005626477524686 2201 1 0.1451117953035066 2201 2 0.10650608514440307 2201 3 0.10747757037214711 2201 4 0.10689195473543833 2201 5 0.09362583707883232 2201 6 0.1349126608793938 2201 7 0.19293522682755793 2201 8 0.12366841121249195 2201 9 0.12181972532486895 2201 10-11 0.1572015339814783 2201 12-13 0.12466978413100094 2201 14-15 0.2536263402978278 2201 16-17 0.2595362545954103 2201 18-19 0.24133573865596958 2201 20-21 0.26122514841259203 2201 22-23 0.3206419148045683 2201 24-25 0.20729202132680768 2201 26-27 0.32685205492599323 2201 28-29 0.325809371080787 2201 30-31 0.3004055301727959 2201 32-33 0.323325289781188 2201 34-35 0.3114140106419683 2201 36-37 0.26183012686735907 2201 38-39 0.38814443794996834 2201 40-41 0.2871147658035511 2201 42-43 0.45431752374243217 2201 44-45 0.303104723545502 2201 46-47 0.3846384770737501 2201 48-49 0.29896883094459525 2201 50-51 0.34731523190788494 2201 52-53 0.19170947671202043 2201 54-55 0.1503061276258819 2201 56-57 0.32409401047782893 2201 58-59 0.2403980328286508 2201 60-61 0.31092129614263087 2201 62-63 0.23898055873991808 2201 64-65 0.23784118515808217 2201 66-67 0.28151331240394484 2201 68-69 0.25713716519270235 2201 70-71 0.3244611671759259 2201 72-73 0.2872259298462225 2201 74-75 0.13431894970495506 2201 76-77 0.1066754354782482 2201 78-79 0.17062391150472678 2201 80-81 0.2512228007840491 2201 82-83 0.3149216387168039 2201 84-85 0.3658117901468856 2201 86-87 0.15458727528490357 2201 88-89 0.32698565131467916 2201 90-91 0.21202731247542062 2201 92-93 0.2166167075640999 2201 94-95 0.21657131537191532 2201 96-97 0.28099290842221336 2201 98-99 0.12882304851456894 2201 100-101 0.29353610965150523 1101 1 0.11239401886859213 1101 2 0.0651560482748792 1101 3 0.07608612072988308 1101 4 0.10602758400638379 1101 5 0.14454952736772952 1101 6 0.1052067385955624 1101 7 0.10606716648399583 1101 8 0.05402937761108717 1101 9 0.08891338633998203 1101 10-11 0.10704149983613576 1101 12-13 0.08357384084351338 1101 14-15 0.16591440708357652 1101 16-17 0.08444452446081385 1101 18-19 0.0912125740350973 1101 20-21 0.14053806197463103 1101 22-23 0.14123787007132194 1101 24-25 0.1833005203955267 1101 26-27 0.19630203793317946 1101 28-29 0.16432555684639993 1101 30-31 0.3062188914045123 1101 32-33 0.2954570315578593 1101 34-35 0.2913057446809262 1101 36-37 0.12516376190077239 1101 38-39 0.10525784257325199 1101 40-41 0.1244262808969907 1101 42-43 0.2113511730622264 1101 44-45 0.09068781307854579 1101 46-47 0.17676219717771602 1101 48-49 0.12315892151673324 1101 50-51 0.12806122084968763 1101 52-53 0.15807373341619524 1101 54-55 0.13014617334231815 1101 56-57 0.14759768900042758 1101 58-59 0.11242812829897986 1101 60-61 0.04262163342557557 1101 62-63 -0.05768139608138512 1101 64-65 -0.0389342088932807 1101 66-67 0.07619771193648717 1101 68-69 0.04125048196802794 1101 70-71 0.0034150132751662454 1101 72-73 0.003722047232407988 1101 74-75 -0.12094119118898305 1101 76-77 -0.2837170142110459 1101 78-79 -0.08345733632680208 1101 80-81 0.10433417159385527 1101 82-83 0.067940148016703 1101 84-85 0.21396858012902698 1101 86-87 0.12191608489174399 1101 88-89 0.02161312714095942 1101 90-91 -0.036595471027261794 1101 92-93 -0.01935006815827478 1101 94-95 -0.027394816132051858 1101 96-97 -0.020586447128671637 1101 98-99 -0.02716813829368192 1101 100-101 -0.02373067396830919 2304 1 0.036371696393132424 2304 2 0.06448324730113342 2304 3 0.051858073769679436 2304 4 0.06114975833534686 2304 5 0.07862945575118374 2304 6 0.045407480816940904 2304 7 0.0577076190380339 2304 8 0.051205335072943114 2304 9 0.059445541201462504 2304 10-11 0.05530968872663067 2304 12-13 0.06312320377637093 2304 14-15 0.07586132280757596 2304 16-17 0.11984846560422113 2304 18-19 0.11947870636937097 2304 20-21 0.16330127790745763 2304 22-23 0.13108932343309476 2304 24-25 0.08112969036571371 2304 26-27 0.1263928521061075 2304 28-29 0.033197996387372086 2304 30-31 0.08502533503254028 2304 32-33 0.15092428392429724 2304 34-35 0.2083896549666875 2304 36-37 0.14418516218291444 2304 38-39 0.21980011457468152 2304 40-41 0.21639970944608677 2304 42-43 0.03934979023753726 2304 44-45 0.12385152705159186 2304 46-47 0.09546157425520363 2304 48-49 0.17116368974936336 2304 50-51 0.013568850580227831 2304 52-53 0.09293580456373007 2304 54-55 0.09885162237880962 2304 56-57 0.11229738991573157 2304 58-59 0.10855254343672271 2304 60-61 0.1382364925561248 2304 62-63 0.09576305642799099 2304 64-65 0.08029382410840213 2304 66-67 0.15149139933249245 2304 68-69 -0.03012167374956931 2304 70-71 0.02278619096534129 2304 72-73 -0.012499737932849087 2304 74-75 0.06873168609137537 2304 76-77 -0.2983632307059274 2304 78-79 0.09866560665168578 2304 80-81 0.2244988217187469 2304 82-83 0.3623042999152446 2304 84-85 0.29973709471847343 2304 86-87 0.22854154598839926 2304 88-89 0.19988095776261616 2304 90-91 0.0963258763986019 2304 92-93 0.08255938846420463 2304 94-95 0.20037809167094878 2304 96-97 0.09809557484763332 2304 98-99 -0.018613306410180996 2304 100-101 0.062027022763160744 2202 1 0.07613714032108732 2202 2 0.08081736184063004 2202 3 0.053153699878365046 2202 4 0.06771474950901535 2202 5 0.0370350830252022 2202 6 0.07742722245781408 2202 7 0.045892145545089136 2202 8 0.09949094361528665 2202 9 0.1159583620462854 2202 10-11 0.09386785082043758 2202 12-13 0.12616136202888129 2202 14-15 0.10200488886265902 2202 16-17 0.04148776847483049 2202 18-19 -0.016101161170858802 2202 20-21 0.003737086283187807 2202 22-23 0.07612677601042606 2202 24-25 0.013123211965421433 2202 26-27 0.10899509947655162 2202 28-29 0.05818067821203243 2202 30-31 0.016932265755521314 2202 32-33 -0.024456365253705314 2202 34-35 -0.14923430756131495 2202 36-37 -0.09569597902549276 2202 38-39 -0.006688169490871587 2202 40-41 0.05002734385886498 2202 42-43 -0.08806585244479948 2202 44-45 -0.002700230014873739 2202 46-47 -0.07810093626518011 2202 48-49 -0.03429191313894364 2202 50-51 -0.01665338854654408 2202 52-53 -0.007407645605240987 2202 54-55 -0.027991873108753396 2202 56-57 0.01285666986807854 2202 58-59 -0.09331398900059185 2202 60-61 -0.10273903864248979 2202 62-63 -0.041745683585737936 2202 64-65 -0.13248599120755244 2202 66-67 -0.01661467206370304 2202 68-69 -0.10733389530999915 2202 70-71 -0.20519329050366508 2202 72-73 -0.14542873772999343 2202 74-75 -0.07858313351905366 2202 76-77 -0.46778408071002886 2202 78-79 -0.1288644654696114 2202 80-81 0.0527091312662904 2202 82-83 0.2111972854607771 2202 84-85 0.17845281444241579 2202 86-87 0.09791125072700169 2202 88-89 0.10356249971684406 2202 90-91 0.024080626218406564 2202 92-93 0.09425091021928722 2202 94-95 0.0710722861438029 2202 96-97 0.12623612613748492 2202 98-99 0.044068102825313815 2202 100-101 0.2090737073846185 1102 1 0.05116576255991134 1102 2 0.060415351802291184 1102 3 0.05577875791082931 1102 4 -0.0029988287957678494 1102 5 -0.0022611594572410354 1102 6 0.0034842071964931165 1102 7 0.006150780147578416 1102 8 -0.010498733182700448 1102 9 0.00514841015723988 1102 10-11 0.024966816817617143 1102 12-13 -0.0010096267874075693 1102 14-15 -0.10911650094936931 1102 16-17 -0.13877622916856325 1102 18-19 -0.1057634495612163 1102 20-21 -0.22383465534952762 1102 22-23 -0.151719901451834 1102 24-25 -0.02495612620263188 1102 26-27 -0.11181448271103989 1102 28-29 -0.18732596800098378 1102 30-31 -0.13990326520804075 1102 32-33 -0.18281084187614027 1102 34-35 -0.08278293076704557 1102 36-37 -0.1456580194551904 1102 38-39 -0.21329389901018203 1102 40-41 -0.21484268238283022 1102 42-43 -0.22589671450563742 1102 44-45 -0.27491635440139817 1102 46-47 -0.23685456010959882 1102 48-49 -0.257316800919142 1102 50-51 -0.23073244920151126 1102 52-53 -0.3002724846512095 1102 54-55 -0.2866341480903216 1102 56-57 -0.30337818866542676 1102 58-59 -0.3493634659559888 1102 60-61 -0.2812584779475813 1102 62-63 -0.38989531598162586 1102 64-65 -0.4419285631826213 1102 66-67 -0.29159858057112586 1102 68-69 -0.11475852538934106 1102 70-71 -0.3625447149562433 1102 72-73 -0.4327528244192891 1102 74-75 -0.5359968147477687 1102 76-77 -0.5212043108681392 1102 78-79 -0.23420763491539986 1102 80-81 -0.07647524927404703 1102 82-83 -0.045460806581139934 1102 84-85 -0.1710306462759732 1102 86-87 -0.14041243403047332 1102 88-89 -0.17881733368525943 1102 90-91 -0.1307661005373859 1102 92-93 -0.27056126722508367 1102 94-95 -0.3546285350910807 1102 96-97 -0.15809788158501448 1102 98-99 -0.32550149240609727 1102 100-101 -0.3060837558093503 2305 1 0.034860787065447596 2305 2 0.05868526224152504 2305 3 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-0.12559293341620759 1213 70-71 -0.14067162776056108 1213 72-73 -0.1148044819300793 1213 74-75 -0.055341349789820526 1213 76-77 0.0703237419735494 1213 78-79 -0.29742846215065555 1213 80-81 -0.3845310723086328 1213 82-83 -0.3959521152848069 1213 84-85 -0.3398427600620906 1213 86-87 -0.36525427575495684 1213 88-89 -0.5397012446510914 1213 90-91 -0.40122295281100406 1213 92-93 -0.27308312831084436 1213 94-95 -0.14372081653144164 1213 96-97 -0.3171471860188646 1213 98-99 -0.24430894676962822 1213 100-101 0.019662695680615627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 32.0 9 112.0 10 211.0 11 166.0 12 161.0 13 283.0 14 519.0 15 1123.0 16 2181.0 17 3513.0 18 5066.0 19 6701.0 20 9023.0 21 11161.0 22 13978.0 23 17502.0 24 21503.0 25 26818.0 26 32746.0 27 40847.0 28 52390.0 29 68303.0 30 81240.0 31 98496.0 32 130671.0 33 185134.0 34 282233.0 35 488301.0 36 948024.0 37 1498698.0 38 450796.0 39 266.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.091028402930114 33.186262493603344 15.790809753688587 17.931899349777954 2 30.14173319074638 25.798517842983 29.99484391395388 14.064905052316746 3 31.595317601024213 23.712488786749148 25.3511140449861 19.341079567240538 4 27.6251376485661 23.546229037834568 29.351196512463794 19.477436801135536 5 29.611341870835243 21.87660988124031 29.944153938532935 18.567894309391512 6 27.495515500302243 22.199105310768626 31.516302409694248 18.789076779234883 7 30.85876187390344 23.346239551891685 25.110340018083143 20.68465855612173 8 27.240792791215583 26.964141643422597 25.277817910443094 20.517247654918723 9 26.229226321361494 24.172039543540123 28.644257529191957 20.954476605906432 10-11 22.425535421239278 29.229481111061734 31.022242627232398 17.322740840466587 12-13 22.034805713856333 29.01172490786759 30.056497011132706 18.896972367143373 14-15 21.787120860755977 29.028920752364286 30.498036824145757 18.68592156273398 16-17 22.091283043667293 28.396792195440895 30.748047821860684 18.763876939031125 18-19 21.217684861539325 28.399114555548287 31.24817030402597 19.13503027888642 20-21 21.759029235610804 28.75458079948586 30.7776914088084 18.708698556094934 22-23 21.29642800439552 29.820316078100184 30.190476211743256 18.692779705761044 24-25 21.174522590258032 28.958002680763094 30.65191893399193 19.215555794986937 26-27 21.000298557256116 28.92881363350433 30.49639554080063 19.574492268438924 28-29 21.08968709100503 29.705587263462597 30.292619606569865 18.912106038962502 30-31 20.48099427381921 29.649951107620627 30.695493259238138 19.17356135932203 32-33 20.36326848068299 30.066301148027456 30.32355835588516 19.246872015404392 34-35 20.871094945896825 30.106808467592916 30.406649720881347 18.615446865628915 36-37 20.80584555903339 29.797220000969137 30.76223878193999 18.634695658057485 38-39 20.769538416739763 29.118908871338 31.114106890520908 18.997445821401328 40-41 20.623328151939514 29.36905718655452 29.894145039874253 20.11346962163171 42-43 20.919071779461536 29.54361381116488 30.341422664403556 19.195891744970027 44-45 20.509178078903396 29.214522137534132 30.53514276670468 19.741157016857787 46-47 20.947868261650505 29.367936349814748 30.046517942786604 19.637677445748142 48-49 20.563720720216764 30.06006942502108 30.205344112087612 19.170865742674547 50-51 21.143491902358242 29.377148539927937 29.865582864849422 19.6137766928644 52-53 20.544870152280534 29.249503617509866 29.635421952750796 20.570204277458803 54-55 21.04676087815294 29.454167979543023 29.884921688553646 19.61414945375039 56-57 20.568352133213086 29.268779344164585 30.081843484315595 20.081025038306734 58-59 20.672793535132836 29.526400221173795 30.107271747314297 19.693534496379073 60-61 20.81989016799937 29.690972445530615 29.87641890153708 19.612718484932937 62-63 20.29447638222195 29.759586097122202 29.664100280651486 20.281837240004357 64-65 20.685812362268262 29.572727214870387 29.789123127052125 19.952337295809226 66-67 20.623356199426997 29.871506355005316 29.539167481239147 19.965969964328536 68-69 20.491455482350652 30.065163085254014 29.746359965358316 19.697021467037025 70-71 20.535057169710466 30.59742199483693 29.41320384312306 19.45431699232954 72-73 20.39765152810235 30.529833188194306 29.358243574736807 19.714271708966532 74-75 20.544900941719177 30.06764754225766 29.36276510976004 20.024686406263122 76-77 20.651725339216963 29.753916962188136 29.489923983242367 20.104433715352528 78-79 20.378845602428957 29.58486564900361 29.873322119923927 20.162966628643503 80-81 20.405714160023322 29.808511571918782 29.61706892214528 20.168705345912617 82-83 20.060870398237864 29.910435056204467 29.528350992574477 20.500343552983193 84-85 20.075756433853805 29.989543714030347 29.602610889387233 20.332088962728616 86-87 20.17878939322278 29.730402369924153 30.000113887128478 20.090694349724593 88-89 20.32450498095226 30.12015072045099 29.63913917032291 19.91620512827384 90-91 20.595043939407812 29.86058553272303 29.230732640578527 20.313637887290632 92-93 20.23850493404193 30.136715404479332 29.211712350503117 20.41306731097562 94-95 19.775581508309333 30.498571283410698 29.17505155997539 20.55079564830458 96-97 19.94975552774618 30.647665272388892 29.038426256834832 20.3641529430301 98-99 20.133419282540004 30.28255708641569 28.931670939003272 20.65235269204104 100-101 20.183382977782603 30.136358093921135 28.855149422422677 20.825109505873588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 49.5 2 41.5 3 75.0 4 92.0 5 76.0 6 65.0 7 65.0 8 65.5 9 67.0 10 78.5 11 89.0 12 93.5 13 105.5 14 113.5 15 136.5 16 183.0 17 257.5 18 402.5 19 641.0 20 971.5 21 1461.5 22 2291.0 23 3705.0 24 5727.5 25 8630.5 26 13085.5 27 18662.0 28 25049.0 29 32406.0 30 40362.0 31 48949.0 32 62621.5 33 85299.5 34 113350.0 35 149376.0 36 195100.0 37 250379.5 38 302014.0 39 316803.5 40 321517.0 41 308802.0 42 274543.0 43 261188.5 44 245292.0 45 230890.0 46 233459.0 47 215540.0 48 170843.5 49 128568.0 50 97697.5 51 78186.5 52 62630.0 53 47530.5 54 36531.5 55 27812.0 56 19078.0 57 12501.0 58 8205.5 59 5581.0 60 3991.0 61 2920.5 62 1975.0 63 1225.0 64 797.5 65 551.0 66 380.5 67 255.5 68 179.5 69 142.0 70 110.0 71 81.0 72 58.0 73 43.0 74 31.0 75 20.5 76 18.5 77 14.5 78 11.0 79 6.0 80 3.0 81 4.5 82 4.0 83 1.5 84 1.5 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1588806938854715 2 0.0 3 0.005403953326814349 4 0.007011741093469859 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.349557847198977E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3398231388795907E-4 24-25 1.116519282399659E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0011388496680476523 40-41 0.0 42-43 0.0 44-45 1.2281712106396248E-4 46-47 8.597198474477375E-4 48-49 0.0030034368696550827 50-51 3.684513631918874E-4 52-53 0.0011946756321676352 54-55 0.002724307049055168 56-57 0.004566563865014605 58-59 0.006174351631670114 60-61 0.0020320650939673795 62-63 0.0011388496680476523 64-65 0.0015296314168875326 66-67 0.008798171945309312 68-69 0.008061269218925539 70-71 0.00979187410664501 72-73 0.006978245514997869 74-75 0.009713717756877033 76-77 0.005426283712462343 78-79 0.005158319084686424 80-81 0.0022218733719753213 82-83 0.0 84-85 7.927286905037578E-4 86-87 0.00189808278007942 88-89 0.005448614098110336 90-91 0.007413688035133736 92-93 0.00969138737122904 94-95 0.009110797344381218 96-97 0.011087036474228614 98-99 0.008139425568693515 100-101 0.008061269218925539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4478203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.766230199456075 #Duplication Level Percentage of deduplicated Percentage of total 1 90.43651553572431 44.10247935051817 2 4.369074599488596 4.261265953545144 3 1.450279509183958 2.121739931952572 4 0.73721563163925 1.438049087966283 5 0.47683679236400595 1.1626766391996677 6 0.33715403354400375 0.9865038727489213 7 0.28603108100441493 0.9764060278322396 8 0.1765099293705914 0.6886179078540794 9 0.13425778988500217 0.5892521658841998 >10 1.2919403871418644 12.860897122434181 >50 0.1571248078702825 5.25265928390802 >100 0.11816003401748396 10.954956890070216 >500 0.0177886653455468 6.095700512125093 >1k 0.011065425459243643 7.878583482110502 >5k 0.0 0.0 >10k+ 4.5777961402007205E-5 0.6302117718505094 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 28230 0.6303867868428474 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.36902726383775E-4 0.0 0.0 2 8.485546546237408E-4 0.0 2.233038564799318E-5 3 0.00147380545276755 0.0 2.233038564799318E-5 4 0.0021883777935033316 0.0 2.233038564799318E-5 5 0.0030369324481270723 0.0 2.233038564799318E-5 6 0.004979675999502479 0.0 4.466077129598636E-5 7 0.006364159909678056 0.0 4.466077129598636E-5 8 0.008195251532813497 0.0 4.466077129598636E-5 9 0.009736048142525025 0.0 4.466077129598636E-5 10-11 0.01352104850985987 0.0 4.466077129598636E-5 12-13 0.019639574177410003 0.0 4.466077129598636E-5 14-15 0.028046964373879435 0.0 4.466077129598636E-5 16-17 0.03985973838166783 0.0 6.699115694397954E-5 18-19 0.055144887357719156 0.0 8.932154259197272E-5 20-21 0.07088780923955434 0.0 8.932154259197272E-5 22-23 0.08992446300446853 0.0 8.932154259197272E-5 24-25 0.10987666258095044 0.0 1.004867354159693E-4 26-27 0.1317157797446878 0.0 1.116519282399659E-4 28-29 0.15537482333873653 0.0 1.3398231388795907E-4 30-31 0.18026203814342495 0.0 1.5631269953595225E-4 32-33 0.20605363356685708 0.0 1.5631269953595225E-4 34-35 0.23219134996783308 0.0 1.7864308518394543E-4 36-37 0.26117619053892827 0.0 1.7864308518394543E-4 38-39 0.2932091287509745 0.0 1.7864308518394543E-4 40-41 0.32832366018244374 0.0 1.7864308518394543E-4 42-43 0.3654925870935284 0.0 1.7864308518394543E-4 44-45 0.4071834170983316 0.0 1.7864308518394543E-4 46-47 0.45095097296839826 0.0 1.7864308518394543E-4 48-49 0.4982578949636718 0.0 1.7864308518394543E-4 50-51 0.5495731211827601 0.0 1.7864308518394543E-4 52-53 0.6040704273566875 0.0 1.7864308518394543E-4 54-55 0.6619731173419339 0.0 1.7864308518394543E-4 56-57 0.7224661320623473 0.0 1.8980827800794202E-4 58-59 0.7854489847825121 0.0 2.009734708319386E-4 60-61 0.8540144339146751 0.0 2.009734708319386E-4 62-63 0.9250362254681175 0.0 2.009734708319386E-4 64-65 0.9974425009317354 0.0 2.009734708319386E-4 66-67 1.074471166224488 0.0 2.009734708319386E-4 68-69 1.1532750971762558 0.0 2.009734708319386E-4 70-71 1.2370363737418781 0.0 2.009734708319386E-4 72-73 1.325777326307003 0.0 2.121386636559352E-4 74-75 1.413904193266808 0.0 2.233038564799318E-4 76-77 1.5113874918131223 2.233038564799318E-5 2.5679943495192153E-4 78-79 1.611014507381644 2.233038564799318E-5 2.6796462777591815E-4 80-81 1.718825609290155 2.233038564799318E-5 3.014602062479079E-4 82-83 1.8305221983014168 2.233038564799318E-5 3.126253990719045E-4 84-85 1.950983463679516 2.233038564799318E-5 3.126253990719045E-4 86-87 2.076134110043694 2.233038564799318E-5 3.349557847198977E-4 88-89 2.2074479428467177 2.233038564799318E-5 3.4612097754389425E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTCCG 20 0.0020620914 71.35759 1 CGTCGAG 25 0.004992317 57.086075 1 CGAGCGG 80 7.094059E-11 53.518192 1 CGGACGG 55 8.111256E-7 51.89643 1 CGACGCC 75 2.3574103E-9 50.743176 1 CGAGGCG 130 0.0 47.571728 1 GCGCGTT 95 3.8562575E-10 44.998005 4 CGATCGC 45 9.5568306E-4 42.285984 1 CGCGACA 50 0.0016043786 38.057384 1 CGCGAGG 95 8.6052387E-7 35.052853 1 CGAGTGG 165 1.8189894E-12 34.597622 1 AACCGCG 55 0.0025804155 34.545467 2 GGACGGG 375 0.0 32.983063 1 GTGTTTG 56900 0.0 32.213333 1 CGACGGG 105 1.8810188E-6 31.714485 1 AGCGCCC 155 1.3369572E-9 30.691437 1 GGGCCGC 140 1.708213E-8 30.535725 2 TGTTTGA 60505 0.0 30.248339 2 GAGCGCG 110 2.7357764E-6 30.227285 2 CGTCTTC 5395 0.0 29.715687 52-53 >>END_MODULE