FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478203
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT282300.6303867868428474TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG200.002062091471.357591
CGTCGAG250.00499231757.0860751
CGAGCGG807.094059E-1153.5181921
CGGACGG558.111256E-751.896431
CGACGCC752.3574103E-950.7431761
CGAGGCG1300.047.5717281
GCGCGTT953.8562575E-1044.9980054
CGATCGC459.5568306E-442.2859841
CGCGACA500.001604378638.0573841
CGCGAGG958.6052387E-735.0528531
CGAGTGG1651.8189894E-1234.5976221
AACCGCG550.002580415534.5454672
GGACGGG3750.032.9830631
GTGTTTG569000.032.2133331
CGACGGG1051.8810188E-631.7144851
AGCGCCC1551.3369572E-930.6914371
GGGCCGC1401.708213E-830.5357252
TGTTTGA605050.030.2483392
GAGCGCG1102.7357764E-630.2272852
CGTCTTC53950.029.71568752-53
Produced by FastQC (version 0.11.2)