FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064296
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3403776.721111878136665TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG451350.081.1943
GATCGGA453400.081.014541
CGGAAGA453850.080.54714
ATCGGAA455200.080.5281452
GAAGAGC468100.078.089666
GGAAGAG507200.072.200855
AGAGCAC532500.068.600988
GAGCACA532000.068.567259
AAGAGCA550000.066.418237
ATGCCGT396150.045.34675248-49
GCCGTCT397750.045.34346450-51
TCGTATG398750.045.24121544-45
TGCCGTC397650.045.1338950-51
CGTATGC397150.045.1129746-47
TATGCCG396700.045.08631548-49
GTATGCC399850.045.01029246-47
CGTATCT396250.045.0038838-39
TATCTCG396900.044.984740-41
TCTCGTA398050.044.93296442-43
AATTCGT392900.044.89800634-35
Produced by FastQC (version 0.11.2)