##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5064296 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.661754960610516 33.0 33.0 33.0 33.0 33.0 2 32.688923988645215 33.0 33.0 33.0 33.0 33.0 3 32.726791048548506 33.0 33.0 33.0 33.0 33.0 4 36.65458377630375 37.0 37.0 37.0 37.0 37.0 5 36.660834990687746 37.0 37.0 37.0 37.0 37.0 6 36.54331164687056 37.0 37.0 37.0 37.0 37.0 7 36.4312155134692 37.0 37.0 37.0 37.0 37.0 8 36.37349949529016 37.0 37.0 37.0 37.0 37.0 9 36.42801408132542 37.0 37.0 37.0 37.0 37.0 10-11 36.3488590516826 37.0 37.0 37.0 37.0 37.0 12-13 36.34496038541191 37.0 37.0 37.0 37.0 37.0 14-15 38.2007873354954 40.0 37.0 40.0 37.0 40.0 16-17 38.204122349878446 40.0 37.0 40.0 37.0 40.0 18-19 38.14720950355193 40.0 37.0 40.0 37.0 40.0 20-21 38.14490365492064 40.0 37.0 40.0 37.0 40.0 22-23 37.95539903275795 40.0 37.0 40.0 33.0 40.0 24-25 37.98363020250001 40.0 37.0 40.0 33.0 40.0 26-27 37.91057957512752 40.0 37.0 40.0 33.0 40.0 28-29 37.705551867426394 40.0 37.0 40.0 33.0 40.0 30-31 37.70094925336118 40.0 37.0 40.0 33.0 40.0 32-33 37.535274893094716 40.0 37.0 40.0 33.0 40.0 34-35 37.35849444819181 40.0 37.0 40.0 33.0 40.0 36-37 37.02182188797811 37.0 37.0 40.0 33.0 40.0 38-39 37.17535951690027 40.0 37.0 40.0 33.0 40.0 40-41 36.97409511608326 38.5 37.0 40.0 33.0 40.0 42-43 36.770431467670925 37.0 37.0 40.0 33.0 40.0 44-45 36.768655702589264 37.0 37.0 40.0 33.0 40.0 46-47 36.764996655013846 37.0 37.0 40.0 33.0 40.0 48-49 36.448208201100414 37.0 37.0 40.0 33.0 40.0 50-51 36.368704554394135 37.0 37.0 40.0 33.0 40.0 52-53 36.261571499770156 37.0 37.0 40.0 33.0 40.0 54-55 35.94467730559193 37.0 37.0 40.0 30.0 40.0 56-57 35.81716343199528 37.0 37.0 40.0 27.0 40.0 58-59 35.54244736089676 37.0 33.0 40.0 27.0 40.0 60-61 35.24602886561133 37.0 33.0 40.0 27.0 40.0 62-63 35.00619809742558 37.0 33.0 38.5 27.0 40.0 64-65 34.72084086317229 37.0 33.0 37.0 27.0 40.0 66-67 34.12942262853514 37.0 33.0 37.0 27.0 40.0 68-69 33.95085832265728 37.0 33.0 37.0 27.0 40.0 70-71 33.44901324883064 37.0 33.0 37.0 27.0 40.0 72-73 32.62863278528743 37.0 33.0 37.0 22.0 37.0 74-75 31.895728350001658 37.0 33.0 37.0 18.5 37.0 76-77 29.30904473198249 33.0 27.0 35.0 15.0 37.0 78-79 31.509350065635974 33.0 33.0 37.0 15.0 37.0 80-81 31.835418684057963 37.0 33.0 37.0 15.0 37.0 82-83 31.74505143459229 37.0 33.0 37.0 15.0 37.0 84-85 31.561175136682373 33.0 33.0 37.0 15.0 37.0 86-87 31.3878734576336 33.0 33.0 37.0 15.0 37.0 88-89 31.245298852989634 33.0 33.0 37.0 15.0 37.0 90-91 31.09663524801868 33.0 33.0 37.0 15.0 37.0 92-93 30.982488089163823 33.0 33.0 37.0 15.0 37.0 94-95 30.80423488279516 33.0 33.0 37.0 15.0 37.0 96-97 30.641595890129643 33.0 33.0 37.0 15.0 37.0 98-99 30.497095252726147 33.0 27.0 37.0 15.0 37.0 100-101 28.934293137683895 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1520224591137591 2306 2 0.17433474242290004 2306 3 0.14985214858361928 2306 4 0.15808468351882254 2306 5 0.14904516846890203 2306 6 0.16035782128824394 2306 7 0.2166276178341917 2306 8 0.23636962773353076 2306 9 0.25553973529506635 2306 10-11 0.2405130984392656 2306 12-13 0.29071098838207377 2306 14-15 0.4409677028488872 2306 16-17 0.37747349336960667 2306 18-19 0.37543834470838533 2306 20-21 0.39507190596589936 2306 22-23 0.4350910470430307 2306 24-25 0.4442006683516695 2306 26-27 0.4322326852427878 2306 28-29 0.435484796298546 2306 30-31 0.4992919102344331 2306 32-33 0.5256659125975105 2306 34-35 0.5176890030839871 2306 36-37 0.461184469855624 2306 38-39 0.4914216324767082 2306 40-41 0.6521447275701533 2306 42-43 0.5483900908062935 2306 44-45 0.5192413902767541 2306 46-47 0.3927225797843974 2306 48-49 0.4373129399537561 2306 50-51 0.548211225255109 2306 52-53 0.5910726461858857 2306 54-55 0.5839323632739379 2306 56-57 0.538607437256239 2306 58-59 0.5737380168253097 2306 60-61 0.5470295056622234 2306 62-63 0.39492360478804045 2306 64-65 0.42115629262081455 2306 66-67 0.37101079479418786 2306 68-69 0.3647852699434253 2306 70-71 0.557902690096526 2306 72-73 0.37386526663983943 2306 74-75 0.4348762080914774 2306 76-77 0.02057932065290302 2306 78-79 0.3167184761028956 2306 80-81 0.5501515086872111 2306 82-83 0.6091385855184974 2306 84-85 0.6886859833691545 2306 86-87 0.6792763536854238 2306 88-89 0.6640352959176887 2306 90-91 0.6217197301086621 2306 92-93 0.5600716447591729 2306 94-95 0.5319409612636683 2306 96-97 0.6050554733764457 2306 98-99 0.5739425658656394 2306 100-101 0.46245869918813653 2307 1 0.09678631965819307 2307 2 0.08139537074885084 2307 3 0.10025205016202676 2307 4 0.16585049631856208 2307 5 0.11401717025002256 2307 6 0.12300529404138416 2307 7 0.12556983544751432 2307 8 0.22621741766075587 2307 9 0.16384922239312516 2307 10-11 0.18295721906871876 2307 12-13 0.20912993828497406 2307 14-15 0.35083709274001507 2307 16-17 0.2940991510389779 2307 18-19 0.2433189202646986 2307 20-21 0.2656221459607693 2307 22-23 0.3907327214928884 2307 24-25 0.38962532006608797 2307 26-27 0.42779136826651154 2307 28-29 0.4084859411448818 2307 30-31 0.4088406778785867 2307 32-33 0.39003042781014585 2307 34-35 0.42490142855474744 2307 36-37 0.391120136813754 2307 38-39 0.4489314866975249 2307 40-41 0.3235266130020804 2307 42-43 0.40170640611525243 2307 44-45 0.33658522333239205 2307 46-47 0.262186542524951 2307 48-49 0.3191376743105252 2307 50-51 0.277364038968976 2307 52-53 0.33081532395137714 2307 54-55 0.15736393940721172 2307 56-57 0.26664619686036417 2307 58-59 0.3316175621901962 2307 60-61 0.2612281189053789 2307 62-63 0.3070320291712818 2307 64-65 0.32788902634300854 2307 66-67 0.2518071469516414 2307 68-69 0.18872623032589786 2307 70-71 0.21574394384232676 2307 72-73 0.04444371628405008 2307 74-75 0.016544561944911607 2307 76-77 -0.22227305234904549 2307 78-79 0.12297746367163853 2307 80-81 0.31386976325346794 2307 82-83 0.34274516790244647 2307 84-85 0.3769012107902938 2307 86-87 0.2650757254673195 2307 88-89 0.2935284417391557 2307 90-91 0.21465690233822343 2307 92-93 0.2588372881307457 2307 94-95 0.3538510184577355 2307 96-97 0.3407724936995997 2307 98-99 -0.0021283081822502936 2307 100-101 0.014770286968534663 2201 1 0.10571119426076336 2201 2 0.10737233500300647 2201 3 0.10018597110244087 2201 4 0.1432380893448979 2201 5 0.09872617029433428 2201 6 0.09623067023367327 2201 7 0.1100743596349929 2201 8 0.1687090285485482 2201 9 0.13448274614619748 2201 10-11 0.1328324327752739 2201 12-13 0.18596044282342206 2201 14-15 0.29617430474862516 2201 16-17 0.23857045999512394 2201 18-19 0.23648705849547014 2201 20-21 0.2561457849485933 2201 22-23 0.29074033522653764 2201 24-25 0.22976689731594035 2201 26-27 0.2883381973119654 2201 28-29 0.3345395902113424 2201 30-31 0.36678613095175905 2201 32-33 0.42902382092275104 2201 34-35 0.36528264743456873 2201 36-37 0.2253347152548173 2201 38-39 0.29325087747661627 2201 40-41 0.3907519978521279 2201 42-43 0.3479569478616895 2201 44-45 0.3135072709225568 2201 46-47 0.29057825715305086 2201 48-49 0.317791965986288 2201 50-51 0.38388246445904173 2201 52-53 0.3023831852357688 2201 54-55 0.3529052453127761 2201 56-57 0.37448314786306014 2201 58-59 0.3623090572677583 2201 60-61 0.27101603186130774 2201 62-63 0.2276841925665849 2201 64-65 0.15058178494384578 2201 66-67 0.31424625618045354 2201 68-69 0.15282881303676277 2201 70-71 0.26089433927266015 2201 72-73 0.2227600956914273 2201 74-75 0.2629512106951175 2201 76-77 0.11944824731274295 2201 78-79 0.36783991227893154 2201 80-81 0.5673893325860639 2201 82-83 0.49488812835636153 2201 84-85 0.5676938004630188 2201 86-87 0.49310889435419014 2201 88-89 0.5134800397829054 2201 90-91 0.3503285431411136 2201 92-93 0.2095904366260548 2201 94-95 0.3180928255455875 2201 96-97 0.45799814592156096 2201 98-99 0.27978552217765085 2201 100-101 0.21120881663688706 1101 1 0.0902975826788861 1101 2 0.09962737713449599 1101 3 0.03732621768026689 1101 4 0.09144209392934499 1101 5 0.08392488663911735 1101 6 0.009247435674701876 1101 7 0.1154150763474675 1101 8 0.017211061431332553 1101 9 0.10806554717814265 1101 10-11 0.08637425462912773 1101 12-13 0.06747920899499604 1101 14-15 0.17565499210380864 1101 16-17 0.1594672801271102 1101 18-19 0.027026411172123233 1101 20-21 0.1337323608831653 1101 22-23 0.21019512739732704 1101 24-25 0.21610767844772028 1101 26-27 0.17423463448781007 1101 28-29 0.11339298162386768 1101 30-31 0.1550454304991291 1101 32-33 0.12948835763366162 1101 34-35 0.11299903459300964 1101 36-37 -0.029844120671043584 1101 38-39 0.07797925822435303 1101 40-41 0.1253836577723746 1101 42-43 0.22682794836704545 1101 44-45 0.08782596208754256 1101 46-47 0.09973045540743186 1101 48-49 -0.04175932768778523 1101 50-51 0.04003139661999455 1101 52-53 0.07285985597237499 1101 54-55 0.040436658338265374 1101 56-57 0.006321426919008388 1101 58-59 -0.06682749203213945 1101 60-61 -0.0010833222346420257 1101 62-63 -0.07921843977174348 1101 64-65 -0.13648087713071533 1101 66-67 -0.06791644471793745 1101 68-69 -0.11567313602053986 1101 70-71 -0.29370010672644753 1101 72-73 -0.23024119533583587 1101 74-75 -0.22246952834938227 1101 76-77 -0.3263604728110785 1101 78-79 -0.09087158785386862 1101 80-81 0.13096128683021035 1101 82-83 0.15899557241519702 1101 84-85 0.18660191269132653 1101 86-87 0.24549229396898653 1101 88-89 0.16177429455656878 1101 90-91 0.0054145854059193255 1101 92-93 0.05770285325845137 1101 94-95 0.1266318633599539 1101 96-97 0.06918148155339665 1101 98-99 0.1580920786279698 1101 100-101 0.09015881978895379 2304 1 0.07820420859512467 2304 2 0.08997874750372858 2304 3 0.07329835785262873 2304 4 0.10108749479973511 2304 5 0.09197819618100311 2304 6 0.10646515036226845 2304 7 0.11044851380658116 2304 8 0.17582417553114027 2304 9 0.09080771878821281 2304 10-11 0.061324698364963126 2304 12-13 0.04956581205577493 2304 14-15 0.14109001861297088 2304 16-17 0.09950019056921633 2304 18-19 0.1352877362053846 2304 20-21 0.14896474101700363 2304 22-23 0.07735366619785822 2304 24-25 0.1490601517198371 2304 26-27 0.08665393630899842 2304 28-29 0.12729472190488167 2304 30-31 0.11784750707116132 2304 32-33 0.18300539944756622 2304 34-35 0.14177783513560627 2304 36-37 0.08995570035148148 2304 38-39 0.1160747509998501 2304 40-41 0.19946595033389514 2304 42-43 0.15540532113097782 2304 44-45 0.17190486132605542 2304 46-47 0.2262679181933649 2304 48-49 0.20001886834754146 2304 50-51 0.14523193492725994 2304 52-53 0.21192459657584806 2304 54-55 0.09913010607942141 2304 56-57 0.061364266320055094 2304 58-59 0.12228102952057185 2304 60-61 0.010035643212205514 2304 62-63 0.18183339870524406 2304 64-65 0.04980117716912247 2304 66-67 -0.01387288061642522 2304 68-69 -0.02730842191373739 2304 70-71 0.04136614222889534 2304 72-73 -0.10609207784978025 2304 74-75 -0.052749061924743046 2304 76-77 -0.30588088836712046 2304 78-79 -0.06477093989373373 2304 80-81 0.08752422661862269 2304 82-83 0.2460571692475959 2304 84-85 0.27520472183784506 2304 86-87 0.19634600425210635 2304 88-89 0.06006027065457431 2304 90-91 0.13023791492862458 2304 92-93 0.04251554557800219 2304 94-95 0.11851468164698176 2304 96-97 0.17620611324557345 2304 98-99 0.13688229960849796 2304 100-101 0.02745723057226357 2202 1 0.06297183631694026 2202 2 0.09455230441307094 2202 3 0.0729703827033461 2202 4 0.05171630232732838 2202 5 0.032691598678134426 2202 6 0.054321625421081876 2202 7 0.08891223296937767 2202 8 0.08429950576818612 2202 9 0.10306041798599352 2202 10-11 0.08747864311433773 2202 12-13 0.1332326496213696 2202 14-15 0.06678027435818734 2202 16-17 -0.002993928497076581 2202 18-19 0.08628636398752576 2202 20-21 0.06504419393937155 2202 22-23 -0.028427367036954365 2202 24-25 -0.03604261382694318 2202 26-27 0.07393754810705389 2202 28-29 0.049699948396231264 2202 30-31 0.02526049941087649 2202 32-33 0.07650960657706207 2202 34-35 -0.04848318784527095 2202 36-37 -0.11113755014613957 2202 38-39 0.057505175030250655 2202 40-41 0.07258631344385691 2202 42-43 -0.025011169610323236 2202 44-45 -0.05868918837537507 2202 46-47 -0.07511259329707087 2202 48-49 -0.16050310221763198 2202 50-51 -0.022378445674711145 2202 52-53 0.05958730240852361 2202 54-55 -0.001657788244720848 2202 56-57 -0.014478290528970206 2202 58-59 -0.09691494128587408 2202 60-61 -0.04392757833312544 2202 62-63 -0.18910084039240616 2202 64-65 -0.14491653801272975 2202 66-67 -0.013349628309278216 2202 68-69 -0.1243683320410014 2202 70-71 -0.13234080632047807 2202 72-73 -0.3875991802081913 2202 74-75 -0.30057839421257526 2202 76-77 -0.5416855961299518 2202 78-79 -0.27833629365451884 2202 80-81 -0.1376974393443433 2202 82-83 -0.04686874606695923 2202 84-85 -0.05841612381026451 2202 86-87 -0.17468361152689127 2202 88-89 -0.14239049537473747 2202 90-91 -0.10664911082685435 2202 92-93 -0.10415607213115052 2202 94-95 -0.08269317166141477 2202 96-97 -0.08068664265043779 2202 98-99 -0.18746137311000055 2202 100-101 -0.1163576104917361 1102 1 0.03638681031716828 1102 2 0.06650788337937286 1102 3 0.03953157125445017 1102 4 -0.016549402192097773 1102 5 7.463902129032363E-4 1102 6 0.00904154404854296 1102 7 0.03041992564966023 1102 8 -0.06615994462120511 1102 9 -0.007986500963220067 1102 10-11 0.04834053208510625 1102 12-13 -0.03085538200735982 1102 14-15 -0.10154288308314108 1102 16-17 -0.11981407684973533 1102 18-19 -0.07205045068582905 1102 20-21 -0.1585513772771705 1102 22-23 -0.11003146905016337 1102 24-25 -0.01699375916618351 1102 26-27 -0.08612240013558647 1102 28-29 -0.13856964397994176 1102 30-31 -0.16185179607661837 1102 32-33 -0.06464402733646324 1102 34-35 -0.10544545703891828 1102 36-37 -0.07976328144377476 1102 38-39 -0.012183662366517467 1102 40-41 -0.04695676176000774 1102 42-43 -0.13353578203555116 1102 44-45 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1213 88-89 -0.5435922944983815 1213 90-91 -0.45146065133621605 1213 92-93 -0.2877109482202975 1213 94-95 -0.08188509197377769 1213 96-97 -0.18693508372173184 1213 98-99 -0.22334133140266133 1213 100-101 0.049948049258887295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 19.0 9 55.0 10 59.0 11 44.0 12 75.0 13 197.0 14 639.0 15 1568.0 16 3133.0 17 5272.0 18 7409.0 19 9603.0 20 12675.0 21 16617.0 22 21620.0 23 27640.0 24 35843.0 25 46255.0 26 62023.0 27 90136.0 28 148771.0 29 201338.0 30 144355.0 31 123133.0 32 158975.0 33 222028.0 34 337188.0 35 565941.0 36 1031090.0 37 1423097.0 38 366855.0 39 642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32229932427489 24.199007670874213 20.321864265670513 15.156828739180389 2 29.430823158835896 28.44284773243902 28.569400366803205 13.556928741921878 3 34.06892651328556 18.686220522741664 30.868057482270544 16.376795481702224 4 28.669280180476214 18.569152532733433 28.943217291845713 23.818349994944644 5 37.09370463337846 17.181973565526185 28.93833614780811 16.78598565328725 6 33.005120553774894 17.813216289095266 32.29623623895602 16.885426918173817 7 30.784318294191337 25.340698884899304 25.432695877176215 18.442286943733148 8 28.106216540265414 30.85686539649341 23.049521591944863 17.987396471296307 9 34.11828613493366 22.490352064729233 24.3750562763314 19.016305524005706 10-11 26.453755862611505 29.41399949765969 27.79414947309557 16.33809516663323 12-13 22.64894178734489 28.219076538605382 27.44439797531502 21.68758369873471 14-15 22.208466882662467 28.517487919347527 27.608328976031416 21.66571622195859 16-17 25.67633289997267 24.80543198896747 28.012551004127722 21.505684106932137 18-19 21.139088236548574 24.781499738561884 31.95458954215946 22.124822482730078 20-21 24.867385318709648 25.064520320297234 31.495660996118712 18.572433364874406 22-23 21.046395447338124 32.69356612674235 27.40472998189282 18.855308444026704 24-25 20.90325504339696 24.776449634154407 31.548468246814217 22.77182707563442 26-27 20.829499302568415 25.027150861639996 27.673333075317874 26.47001676047372 28-29 24.431145019959338 29.337898100742926 27.150654306146404 19.080302573151332 30-31 20.40724910234315 26.073693164854504 30.843388696079376 22.675669036722972 32-33 20.209975878187215 29.95795466931633 27.141995649543393 22.690073802953066 34-35 24.133551830303755 30.03094605844524 27.312769632738686 18.522732478512314 36-37 20.186053500822226 30.00466402437772 31.409676685564982 18.399605789235068 38-39 20.328699942775383 26.025975395388933 31.151212004769114 22.49411265706657 40-41 24.09158153472862 25.90519985403697 30.852580496874587 19.150638114359825 42-43 20.416904936046393 29.577131747433405 30.77450449183855 19.231458824681656 44-45 20.18318868808074 25.853405015691745 30.990693358284528 22.972712937942987 46-47 23.973708599797224 25.84574610037851 27.277224042336844 22.903321257487423 48-49 20.198098201970517 29.866996567048204 30.699504655761917 19.235400575219362 50-51 23.951692134806834 26.160658740744097 30.264094409686216 19.623554714762854 52-53 20.222990181694534 25.96580634230702 26.724897486559613 27.086305989438834 54-55 24.09741283803484 25.972307255757276 30.5804682832566 19.349811622951286 56-57 20.443547586872025 25.952106212721056 30.38389929585732 23.2204469045496 58-59 20.378388993806603 26.197983164171006 30.24132105284308 23.18230678917931 60-61 23.95439064306792 26.211231967622282 30.160849915011656 19.673527474298137 62-63 20.131814109603134 26.246990424846466 30.29539598436334 23.325799481187058 64-65 24.21341208698933 26.24997025708052 30.06512045610878 19.471497199821375 66-67 20.662423564423108 33.454586924517734 26.29554661866682 19.587442892392335 68-69 20.777293867331128 33.50066306888482 26.10245137519529 19.619591688588766 70-71 20.609401455887156 33.77517400778142 26.014336116325627 19.60108842000579 72-73 20.360037981881295 33.690169626007616 26.0666606305873 19.883131761523785 74-75 20.667972517494505 33.099210960141995 25.940317258953417 20.292499263410082 76-77 20.66645010831209 32.35041182290134 26.182971404423718 20.800166664362855 78-79 20.60475796618751 31.771583974212074 26.670356711703846 20.953301347896574 80-81 20.48120470600873 31.478435960302964 26.83232802611821 21.2080313075701 82-83 20.08689855411295 31.480308417991367 26.92875771874314 21.504035309152545 84-85 20.250969857769334 31.277477411123233 26.88495200743009 21.586600723677346 86-87 20.1448531726877 31.077133815861302 27.412115737600068 21.36589727385093 88-89 20.19838177720152 31.117248681595093 27.4670946751568 21.217274866046587 90-91 20.483287896406374 30.745335758172004 27.156369807579196 21.615006537842422 92-93 20.25626470529282 30.955847859703223 27.00368917776351 21.78419825724044 94-95 20.164264243034143 30.934510341210377 26.91393455734043 21.98729085841505 96-97 20.413155883038893 31.176291810720123 26.702855541576163 21.707696764664817 98-99 20.59937112221489 30.914795413295693 26.76730435840386 21.71852910608556 100-101 20.612780173907616 30.97147623605511 26.36093778911455 22.054805800922715 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2084.0 1 1269.0 2 515.5 3 784.0 4 837.5 5 601.0 6 522.0 7 515.5 8 517.5 9 535.5 10 517.0 11 499.5 12 504.5 13 490.5 14 485.5 15 508.0 16 515.5 17 565.0 18 651.0 19 720.5 20 833.0 21 1089.5 22 1479.0 23 2127.0 24 3160.5 25 4768.0 26 7900.5 27 12721.5 28 17548.0 29 22403.5 30 27846.5 31 32774.5 32 43108.5 33 63612.5 34 94143.5 35 129135.5 36 174145.5 37 232926.0 38 280256.0 39 296648.0 40 303972.5 41 306254.0 42 301518.5 43 300473.5 44 288172.5 45 267030.0 46 246584.0 47 218298.0 48 185785.0 49 162770.5 50 144890.5 51 131517.0 52 120914.0 53 106085.0 54 88723.0 55 73119.0 56 60882.5 57 51900.5 58 45176.5 59 38412.5 60 31463.5 61 25858.0 62 21405.0 63 17225.0 64 13902.5 65 11164.5 66 8626.0 67 6870.5 68 6030.5 69 5017.0 70 3564.0 71 2478.5 72 1785.0 73 1348.0 74 1059.0 75 812.5 76 600.0 77 441.0 78 314.0 79 206.0 80 128.5 81 89.0 82 60.0 83 38.0 84 32.0 85 24.0 86 15.5 87 10.5 88 6.0 89 4.0 90 2.0 91 3.0 92 4.0 93 3.0 94 2.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14821408543260506 2 0.0 3 0.00523271151607252 4 0.007108589229381537 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.9492162385452985E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1847648715635895E-4 24-25 1.9746081192726493E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.293354100945127E-4 40-41 0.0 42-43 0.0 44-45 1.8758777133090167E-4 46-47 0.0010761614250035937 48-49 0.002784197448174435 50-51 5.430172327999785E-4 52-53 0.0016191786578035722 54-55 0.002320164540145363 56-57 0.0038406127919853026 58-59 0.005420299287403421 60-61 0.0014217178458763074 62-63 8.490814912872391E-4 64-65 0.0012933683181235852 66-67 0.007720717746356058 68-69 0.006763032808508823 70-71 0.00872776788718511 72-73 0.005973189560799764 74-75 0.008293354100945127 76-77 0.004462614349556187 78-79 0.004354010902996191 80-81 0.0017672742667490208 82-83 0.0 84-85 4.442868268363461E-4 86-87 0.0012736222369308587 88-89 0.004383630024785281 90-91 0.006249634697497934 92-93 0.008500687953468755 94-95 0.00754300301562152 96-97 0.009734818028014161 98-99 0.007009858823417905 100-101 0.006772905849105186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5064296.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.00400600692724 #Duplication Level Percentage of deduplicated Percentage of total 1 90.851319064435 48.15483861428576 2 4.754596957823376 5.040253714259765 3 1.414632970868569 2.2494364345654487 4 0.6237623405664955 1.3224761138512608 5 0.4664424329770879 1.236165875970166 6 0.27543813550393575 0.8759594753272469 7 0.21267448488859697 0.7890819772188745 8 0.1603903056607244 0.6801062979755147 9 0.10357186001694828 0.4940751141438259 >10 0.8913841580780861 9.446558824703192 >50 0.1260592063482758 4.610779760379417 >100 0.10481803375578254 12.122360949132265 >500 0.012600664935909672 4.417943309847116 >1k 0.0022718767042164685 1.793117608295785 >5k 0.0 0.0 >10k+ 3.750743693358401E-5 6.766845930044493 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 340377 6.721111878136665 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002330037580741726 0.0 0.0 2 0.002685467042210803 0.0 0.0 3 0.003297595559185324 0.0 0.0 4 0.0036727711018471275 0.0 0.0 5 0.004383630024785281 0.0 0.0 6 0.005133981110108887 0.0 0.0 7 0.00641747638763611 0.0 0.0 8 0.007582495178006973 0.0 0.0 9 0.00833284626333058 0.0 0.0 10-11 0.012025363446370433 0.0 0.0 12-13 0.016231278740421178 0.0 0.0 14-15 0.0213553868099337 0.0 0.0 16-17 0.027298957248944374 0.0 0.0 18-19 0.03397313269208593 0.0 0.0 20-21 0.03951190846664571 0.0 0.0 22-23 0.04490258863226004 0.0 0.0 24-25 0.050757301705903445 0.0 0.0 26-27 0.056937825119226834 0.0 0.0 28-29 0.06388844569906657 0.0 0.0 30-31 0.09475157060329806 0.0 9.873040596363246E-6 32-33 0.10411121308865043 0.0 1.9746081192726493E-5 34-35 0.11149624745473014 0.0 2.961912178908974E-5 36-37 0.1201252849359516 0.0 3.9492162385452985E-5 38-39 0.13048210452153666 0.0 3.9492162385452985E-5 40-41 0.14109562316262714 0.0 3.9492162385452985E-5 42-43 0.15238050856427032 0.0 3.9492162385452985E-5 44-45 0.16566962120697526 0.0 3.9492162385452985E-5 46-47 0.17841571661688022 0.0 3.9492162385452985E-5 48-49 0.1919022900715124 0.0 3.9492162385452985E-5 50-51 0.20778801239106087 0.0 3.9492162385452985E-5 52-53 0.22409827545625294 0.0 3.9492162385452985E-5 54-55 0.24163279555539408 0.0 3.9492162385452985E-5 56-57 0.26065814478458604 0.0 3.9492162385452985E-5 58-59 0.28115457706263614 0.0 3.9492162385452985E-5 60-61 0.3027469168468826 0.0 3.9492162385452985E-5 62-63 0.326866755023798 0.0 3.9492162385452985E-5 64-65 0.35287234395461875 0.0 5.9238243578179475E-5 66-67 0.3804082541778758 0.0 5.9238243578179475E-5 68-69 0.4100767411699474 0.0 5.9238243578179475E-5 70-71 0.44344761838565516 0.0 6.911128417454273E-5 72-73 0.4794052322376101 0.0 7.898432477090597E-5 74-75 0.5178014871168668 0.0 7.898432477090597E-5 76-77 0.5606208641832942 0.0 7.898432477090597E-5 78-79 0.6075276800566161 0.0 7.898432477090597E-5 80-81 0.6595191118370648 0.0 7.898432477090597E-5 82-83 0.7169900811484953 9.873040596363246E-6 7.898432477090597E-5 84-85 0.7796937619759983 1.9746081192726493E-5 7.898432477090597E-5 86-87 0.8495356511546719 1.9746081192726493E-5 7.898432477090597E-5 88-89 0.9239882502918471 1.9746081192726493E-5 9.873040596363246E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 45135 0.0 81.194 3 GATCGGA 45340 0.0 81.01454 1 CGGAAGA 45385 0.0 80.5471 4 ATCGGAA 45520 0.0 80.528145 2 GAAGAGC 46810 0.0 78.08966 6 GGAAGAG 50720 0.0 72.20085 5 AGAGCAC 53250 0.0 68.60098 8 GAGCACA 53200 0.0 68.56725 9 AAGAGCA 55000 0.0 66.41823 7 ATGCCGT 39615 0.0 45.346752 48-49 GCCGTCT 39775 0.0 45.343464 50-51 TCGTATG 39875 0.0 45.241215 44-45 TGCCGTC 39765 0.0 45.13389 50-51 CGTATGC 39715 0.0 45.11297 46-47 TATGCCG 39670 0.0 45.086315 48-49 GTATGCC 39985 0.0 45.010292 46-47 CGTATCT 39625 0.0 45.00388 38-39 TATCTCG 39690 0.0 44.9847 40-41 TCTCGTA 39805 0.0 44.932964 42-43 AATTCGT 39290 0.0 44.898006 34-35 >>END_MODULE