FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387621
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT736542.1742101610540256TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA225250.037.831994
TCGGAAG227750.037.6055343
AGAGCAC229000.037.27148
GAGCACA228950.037.238059
CGTCTTC114300.037.1520952-53
GCCGTCT115050.036.84824450-51
ATCGGAA233650.036.6559372
GAAGAGC235450.036.3310666
GATCGGA237050.036.1846661
ATGCCGT117500.036.05890748-49
CCGTCTT116100.036.00330752-53
TGCCGTC116050.035.9986250-51
TATGCCG118350.035.35844848-49
GCTTGAA116200.034.9710860-61
CGTATGC121100.034.63369846-47
CGGGTCG550.002560733834.59921
CTGCTTG122900.034.5714258-59
AAGAGCA247450.034.473237
TGCTTGA119100.034.2392160-61
TCGTATG124600.034.213644-45
Produced by FastQC (version 0.11.2)