##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3696177 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67385517522565 33.0 33.0 33.0 33.0 33.0 2 32.69837239937373 33.0 33.0 33.0 33.0 33.0 3 32.727154570790304 33.0 33.0 33.0 33.0 33.0 4 36.659655638785694 37.0 37.0 37.0 37.0 37.0 5 36.653304481901166 37.0 37.0 37.0 37.0 37.0 6 36.515462327696966 37.0 37.0 37.0 37.0 37.0 7 36.454257196016314 37.0 37.0 37.0 37.0 37.0 8 36.38058215285686 37.0 37.0 37.0 37.0 37.0 9 36.37194782609166 37.0 37.0 37.0 37.0 37.0 10-11 36.30991332395608 37.0 37.0 37.0 37.0 37.0 12-13 36.29694992961647 37.0 37.0 37.0 35.0 37.0 14-15 38.06313996867574 40.0 37.0 40.0 37.0 40.0 16-17 38.02075969305582 40.0 37.0 40.0 35.0 40.0 18-19 38.01071187878719 40.0 37.0 40.0 33.0 40.0 20-21 37.927839088874805 40.0 37.0 40.0 33.0 40.0 22-23 37.81351068955843 40.0 37.0 40.0 33.0 40.0 24-25 37.75187091960152 40.0 37.0 40.0 33.0 40.0 26-27 37.65466872933845 40.0 37.0 40.0 33.0 40.0 28-29 37.482520588164476 40.0 37.0 40.0 33.0 40.0 30-31 37.40871595164409 40.0 37.0 40.0 33.0 40.0 32-33 37.23616374432285 40.0 37.0 40.0 33.0 40.0 34-35 37.12228215802436 40.0 37.0 40.0 33.0 40.0 36-37 37.023149594838124 40.0 37.0 40.0 33.0 40.0 38-39 36.87254127710875 38.5 37.0 40.0 33.0 40.0 40-41 36.769765760676506 37.0 37.0 40.0 33.0 40.0 42-43 36.61725899490203 37.0 37.0 40.0 33.0 40.0 44-45 36.44301733926703 37.0 37.0 40.0 33.0 40.0 46-47 36.34740949905808 37.0 37.0 40.0 33.0 40.0 48-49 36.13774313297226 37.0 37.0 40.0 33.0 40.0 50-51 35.97710999770844 37.0 37.0 40.0 27.0 40.0 52-53 35.790934525051156 37.0 37.0 40.0 27.0 40.0 54-55 35.57045590619714 37.0 35.0 40.0 27.0 40.0 56-57 35.361972654448095 37.0 33.0 40.0 27.0 40.0 58-59 35.13003949756735 37.0 33.0 40.0 27.0 40.0 60-61 34.872916394425914 37.0 33.0 40.0 27.0 40.0 62-63 34.61642475455045 37.0 33.0 40.0 27.0 40.0 64-65 34.34231342276087 37.0 33.0 38.5 27.0 40.0 66-67 34.03692896741688 37.0 33.0 37.0 27.0 40.0 68-69 33.729391070828044 37.0 33.0 37.0 27.0 40.0 70-71 33.346535352608925 37.0 33.0 37.0 22.0 40.0 72-73 33.01426338619606 37.0 33.0 37.0 22.0 40.0 74-75 32.65782225797087 37.0 33.0 37.0 22.0 38.5 76-77 30.027372201060714 33.0 27.0 35.0 18.5 37.0 78-79 32.41437355408034 35.0 33.0 37.0 22.0 37.0 80-81 32.75970509529171 37.0 33.0 37.0 22.0 37.0 82-83 32.6437688184305 37.0 33.0 37.0 22.0 37.0 84-85 32.42002452804614 37.0 33.0 37.0 22.0 37.0 86-87 32.211511651092465 33.0 33.0 37.0 22.0 37.0 88-89 32.00767306327592 33.0 33.0 37.0 22.0 37.0 90-91 31.80269329634376 33.0 33.0 37.0 22.0 37.0 92-93 31.65076537189642 33.0 33.0 37.0 22.0 37.0 94-95 31.423617294301653 33.0 33.0 37.0 15.0 37.0 96-97 31.234672473747878 33.0 33.0 37.0 15.0 37.0 98-99 31.072704580976506 33.0 33.0 37.0 15.0 37.0 100-101 29.287313486340075 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14588266350926915 2306 2 0.10563587701537358 2306 3 0.11057869272973164 2306 4 0.11779480987703295 2306 5 0.144404519007459 2306 6 0.15709471591738833 2306 7 0.1955323051957336 2306 8 0.1887247887575043 2306 9 0.1069304850901176 2306 10-11 0.12159279403510226 2306 12-13 0.22795234259036334 2306 14-15 0.3336418294848471 2306 16-17 0.2184720823272528 2306 18-19 0.25816230398672246 2306 20-21 0.34918982384260033 2306 22-23 0.37560089853296574 2306 24-25 0.34310954942322525 2306 26-27 0.3645092611764582 2306 28-29 0.32139986999001025 2306 30-31 0.3961633357707015 2306 32-33 0.46978251077926814 2306 34-35 0.4630094703672327 2306 36-37 0.49781277796356704 2306 38-39 0.33102859444473154 2306 40-41 0.32764426409180913 2306 42-43 0.42649693042986314 2306 44-45 0.43930901586219306 2306 46-47 0.35848106976609273 2306 48-49 0.2994103811749511 2306 50-51 0.24580905886216442 2306 52-53 0.43633697106118774 2306 54-55 0.5018667001866106 2306 56-57 0.35565836887830926 2306 58-59 0.4756231217016378 2306 60-61 0.3993823168805122 2306 62-63 0.43261123428487025 2306 64-65 0.4434708521953752 2306 66-67 0.14423918094789911 2306 68-69 0.37222034693278516 2306 70-71 0.3760923794315971 2306 72-73 0.33436618908831406 2306 74-75 0.3614064818745817 2306 76-77 0.001973506437426664 2306 78-79 0.1331240817471837 2306 80-81 0.3056227828003699 2306 82-83 0.29770359137149427 2306 84-85 0.2906573056198525 2306 86-87 0.29660677075089126 2306 88-89 0.1375530520550683 2306 90-91 0.09495599673281774 2306 92-93 0.15862267887587578 2306 94-95 0.01191142182431193 2306 96-97 0.15128283157134703 2306 98-99 0.1509331953058748 2306 100-101 0.08514540009132077 2307 1 0.044337816387034934 2307 2 0.09542105275605905 2307 3 0.11496632016889663 2307 4 0.07515451163932596 2307 5 0.10870924637890056 2307 6 0.16210191376428895 2307 7 0.11820344833478202 2307 8 0.2056592443970402 2307 9 0.15783898909833738 2307 10-11 0.21622461379286761 2307 12-13 0.20574415427471138 2307 14-15 0.3381950742123436 2307 16-17 0.3475776153511987 2307 18-19 0.31431219079733097 2307 20-21 0.2664968656365332 2307 22-23 0.3149056786487634 2307 24-25 0.3536870790602862 2307 26-27 0.34430166641066506 2307 28-29 0.3627226141313429 2307 30-31 0.29926262948857385 2307 32-33 0.2896865441755949 2307 34-35 0.3467675833287629 2307 36-37 0.30610074528738807 2307 38-39 0.36033599771333513 2307 40-41 0.35314897486635033 2307 42-43 0.2586786199793778 2307 44-45 0.251284868791231 2307 46-47 0.17066965827780933 2307 48-49 0.23551199634788844 2307 50-51 0.29351791421814255 2307 52-53 0.1522249082855538 2307 54-55 0.24220112052258713 2307 56-57 0.1307734746518463 2307 58-59 0.20848788586754097 2307 60-61 0.13413445003821778 2307 62-63 0.13054006878221003 2307 64-65 0.08321703227305477 2307 66-67 0.2534817156870943 2307 68-69 0.05279154021415167 2307 70-71 0.167670757901206 2307 72-73 0.07803036082957249 2307 74-75 -0.018833406504292327 2307 76-77 -0.40837391714127236 2307 78-79 -0.058516394414830586 2307 80-81 0.06719067569135007 2307 82-83 0.13830591789002256 2307 84-85 0.17502672570616795 2307 86-87 0.10933317591337044 2307 88-89 0.020086792621739846 2307 90-91 -0.06861094254013977 2307 92-93 -0.02377188690794796 2307 94-95 0.10188820024103507 2307 96-97 0.23822399629338875 2307 98-99 0.12867120774369667 2307 100-101 0.12466797924830075 2201 1 0.06743960327620613 2201 2 0.09678420950427125 2201 3 0.08792796105149137 2201 4 0.0826414703842886 2201 5 0.02185213919045026 2201 6 0.05951774813507171 2201 7 0.09485576360481929 2201 8 0.12783604557508 2201 9 0.08563581695314326 2201 10-11 0.13904279850564905 2201 12-13 0.10322430038847585 2201 14-15 0.27377385940765464 2201 16-17 0.21572270075184008 2201 18-19 0.1602873002612739 2201 20-21 0.182788905891897 2201 22-23 0.23275972188706362 2201 24-25 0.15079440621695284 2201 26-27 0.13757724317508035 2201 28-29 0.31628110266667164 2201 30-31 0.23970191652354345 2201 32-33 0.23781368533892078 2201 34-35 0.1887269919003245 2201 36-37 0.07523506509139821 2201 38-39 0.1564987461450258 2201 40-41 0.10162125571699931 2201 42-43 0.12436299307702114 2201 44-45 0.11458395401973576 2201 46-47 0.1091591015367257 2201 48-49 0.09789337822439137 2201 50-51 0.19017319917749376 2201 52-53 0.2224589273691251 2201 54-55 0.2524925756671905 2201 56-57 0.26991342803843565 2201 58-59 0.23952649844887475 2201 60-61 0.190950880049229 2201 62-63 0.23827689756386405 2201 64-65 0.04002704687245284 2201 66-67 0.19590371169029908 2201 68-69 0.04283102258027327 2201 70-71 -0.018693481743262907 2201 72-73 0.0753133473155998 2201 74-75 -0.004097347887288549 2201 76-77 -0.11058721838599084 2201 78-79 0.15222073480353515 2201 80-81 0.11900757700655618 2201 82-83 0.10351082172347503 2201 84-85 0.11156750739018406 2201 86-87 0.11981930018194475 2201 88-89 0.1256539670254142 2201 90-91 0.12568350247000737 2201 92-93 -0.007107658202780698 2201 94-95 -0.06209090285604546 2201 96-97 0.18683851958732234 2201 98-99 -0.029318943102015993 2201 100-101 -0.11637905375781088 1101 1 0.0910468686408521 1101 2 0.09374599089569102 1101 3 0.06210225072302222 1101 4 0.10901426457963481 1101 5 0.0804384017611639 1101 6 0.08942045469473925 1101 7 0.07751042131665287 1101 8 0.035745156935902855 1101 9 0.07270492449630694 1101 10-11 0.08190782785955975 1101 12-13 0.07942238849226158 1101 14-15 0.1198594402539257 1101 16-17 0.127510398125267 1101 18-19 0.08016458133967319 1101 20-21 0.13080805609965296 1101 22-23 0.06819421044033902 1101 24-25 0.1705238667115978 1101 26-27 0.06754646047588864 1101 28-29 0.08579411565010986 1101 30-31 0.046058656108215246 1101 32-33 0.006735160340653579 1101 34-35 0.042343284432824646 1101 36-37 -0.11331479055344573 1101 38-39 -0.15346715627065777 1101 40-41 -0.0838805105174103 1101 42-43 -0.02919131934138619 1101 44-45 -0.04899547142289862 1101 46-47 -0.02310481444755652 1101 48-49 0.03339905017177358 1101 50-51 -0.04420778366464617 1101 52-53 0.24654631137655514 1101 54-55 0.23104403336449053 1101 56-57 0.024869209686649185 1101 58-59 0.05939679185171087 1101 60-61 0.19760799026475695 1101 62-63 -0.03949705340424714 1101 64-65 -0.026039295141885077 1101 66-67 0.13807245894209785 1101 68-69 0.14705135137597125 1101 70-71 0.10554582739718654 1101 72-73 0.22683825127766966 1101 74-75 0.13188935166879645 1101 76-77 -0.058972298392031774 1101 78-79 0.01748521264238434 1101 80-81 0.20609837190861668 1101 82-83 0.3318225396577148 1101 84-85 0.265109321527099 1101 86-87 0.3394116914554104 1101 88-89 0.06772911919199487 1101 90-91 0.1856775260190453 1101 92-93 0.11534489377586965 1101 94-95 0.004499727864047287 1101 96-97 0.007845872553563282 1101 98-99 0.1466462082805755 1101 100-101 0.21485899476051884 2304 1 0.10263716211984075 2304 2 0.12513100578230762 2304 3 0.07101743059363486 2304 4 0.10606492384403055 2304 5 0.1276054676428373 2304 6 0.12821519848824892 2304 7 0.11126834032451427 2304 8 0.1395569140042454 2304 9 0.15607556764287267 2304 10-11 0.13969280021299113 2304 12-13 0.08278446903668879 2304 14-15 0.14815380348734664 2304 16-17 0.1831617155347729 2304 18-19 0.1723454705071461 2304 20-21 0.1837296942989326 2304 22-23 0.10711627397149215 2304 24-25 0.18130966734287313 2304 26-27 0.2444327513581186 2304 28-29 0.16914377202673592 2304 30-31 0.229662112489315 2304 32-33 0.25689046243821423 2304 34-35 0.33783980342725073 2304 36-37 0.13288100761538857 2304 38-39 0.24731299192391276 2304 40-41 0.30786693189488545 2304 42-43 0.3294613859931701 2304 44-45 0.2910979336945161 2304 46-47 0.3715416004524954 2304 48-49 0.279720768960253 2304 50-51 0.19126657330073016 2304 52-53 0.3702241448703134 2304 54-55 0.1523058699027331 2304 56-57 0.21985449096533216 2304 58-59 0.3431840923374878 2304 60-61 0.29068991142165856 2304 62-63 0.3649333936419339 2304 64-65 0.2848083465048177 2304 66-67 0.2486475524031846 2304 68-69 0.14948581668904382 2304 70-71 0.03273852512054276 2304 72-73 0.15524258518556877 2304 74-75 0.28518231578421904 2304 76-77 -0.17594136109490321 2304 78-79 0.186323776811534 2304 80-81 0.2067293741248193 2304 82-83 0.4734734429282881 2304 84-85 0.4829212129107745 2304 86-87 0.27337612027948666 2304 88-89 0.32110659003437547 2304 90-91 0.18541449183579672 2304 92-93 0.18406537238615428 2304 94-95 0.24780215001712236 2304 96-97 0.1997502960050177 2304 98-99 0.16779807414433634 2304 100-101 0.35862256620507793 2202 1 0.047726082542020265 2202 2 0.0815466915870573 2202 3 0.03590387247528781 2202 4 0.035817992973235846 2202 5 0.03378114869207138 2202 6 0.04655211121514924 2202 7 0.02451897157822458 2202 8 0.0778658520704596 2202 9 0.14263561656700574 2202 10-11 0.04747211562639819 2202 12-13 0.11380529196546263 2202 14-15 0.07586225006055969 2202 16-17 0.007728236638939734 2202 18-19 -0.0692481709767776 2202 20-21 0.004321917685516041 2202 22-23 -0.08038240317651457 2202 24-25 -0.1488336600115403 2202 26-27 0.014549896375569915 2202 28-29 0.008577123028111089 2202 30-31 0.06467826366776563 2202 32-33 0.09574896388445353 2202 34-35 -0.009742090680639137 2202 36-37 -0.0752621540166345 2202 38-39 -0.068160075710189 2202 40-41 -0.05067617011309977 2202 42-43 -0.02742460465522356 2202 44-45 -0.12181078253786382 2202 46-47 -0.21952533203049285 2202 48-49 -0.16695298444032147 2202 50-51 -0.09473886851832702 2202 52-53 0.0019265011455402714 2202 54-55 -0.0746667106966612 2202 56-57 -0.08461560042066196 2202 58-59 -0.05951580917477628 2202 60-61 -0.12783681649324308 2202 62-63 -0.30763912228970014 2202 64-65 -0.3208123221400072 2202 66-67 -0.13736471195465327 2202 68-69 -0.08462543617676488 2202 70-71 -0.19591773646145327 2202 72-73 -0.1872837626486188 2202 74-75 -0.08252325316372122 2202 76-77 -0.48144023728992025 2202 78-79 -0.13924631047643743 2202 80-81 0.004080720461658416 2202 82-83 0.07557600077238646 2202 84-85 0.09613865465237126 2202 86-87 0.06281962628084159 2202 88-89 0.16619105216388164 2202 90-91 0.09963255008271332 2202 92-93 -0.009256780689998578 2202 94-95 -0.06849018518704142 2202 96-97 0.05596833083464148 2202 98-99 -0.020710699010077604 2202 100-101 -0.08348492728423906 1102 1 0.045804727604320306 1102 2 0.04605777317431148 1102 3 -0.05922178249428356 1102 4 -0.0029913812668311834 1102 5 -0.005153726942204173 1102 6 -0.014561785555216034 1102 7 0.028780736771473414 1102 8 0.02272339576069271 1102 9 0.02869609943532936 1102 10-11 0.006149681122373352 1102 12-13 -0.024027127773806 1102 14-15 -0.05085375896771893 1102 16-17 -0.04445495939933153 1102 18-19 0.015245911676508683 1102 20-21 0.0221957421248149 1102 22-23 0.004608207206310055 1102 24-25 0.08225441457795313 1102 26-27 0.010935544259147889 1102 28-29 -0.04479862971124504 1102 30-31 -0.01956745730203835 1102 32-33 -0.01375330130321828 1102 34-35 -0.06618509553811691 1102 36-37 -0.06389136577201526 1102 38-39 -0.02032384495430506 1102 40-41 -0.06002269814588601 1102 42-43 -0.14705179019401982 1102 44-45 -0.07725724959046687 1102 46-47 0.012051542611125399 1102 48-49 -0.09188701061700044 1102 50-51 -0.07472892818186949 1102 52-53 0.03628820574989078 1102 54-55 -0.10368648709485484 1102 56-57 -0.2073521979379862 1102 58-59 -0.07759549307168356 1102 60-61 -0.16292966698302536 1102 62-63 -0.22833657158880527 1102 64-65 -0.19891936201501892 1102 66-67 -0.12761120921367564 1102 68-69 -0.14129792370830074 1102 70-71 -0.03248621141116104 1102 72-73 -0.06346377410123694 1102 74-75 -0.04896273263519646 1102 76-77 -0.33530702034779836 1102 78-79 -0.1528374365203291 1102 80-81 0.07077448942992959 1102 82-83 0.1714623428753015 1102 84-85 -0.04226385248008313 1102 86-87 0.039890629159252455 1102 88-89 -0.062245255242736164 1102 90-91 5.293910668697777E-4 1102 92-93 -0.09993554857345899 1102 94-95 -0.22597105625084168 1102 96-97 -0.14837928983304138 1102 98-99 -0.16600966915522264 1102 100-101 -0.0871646712027001 2305 1 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74-75 0.48875254177462324 1213 76-77 0.5700600871747135 1213 78-79 0.24844090561700938 1213 80-81 -0.0027602918680003086 1213 82-83 0.14965646800146004 1213 84-85 0.13286285971401668 1213 86-87 0.16646141732562114 1213 88-89 0.002945107971797256 1213 90-91 0.2461452042034118 1213 92-93 0.2962318630478329 1213 94-95 0.3154111775644175 1213 96-97 0.2435919518599512 1213 98-99 0.14696483635656676 1213 100-101 0.47448522196949483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 42.0 9 115.0 10 237.0 11 160.0 12 232.0 13 451.0 14 1048.0 15 2243.0 16 3894.0 17 5934.0 18 8385.0 19 10903.0 20 13632.0 21 16860.0 22 20484.0 23 24451.0 24 29977.0 25 35432.0 26 42703.0 27 51760.0 28 64182.0 29 82422.0 30 97447.0 31 112509.0 32 144490.0 33 194147.0 34 277938.0 35 423140.0 36 673466.0 37 966550.0 38 389577.0 39 1362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30391031539836 24.651695026397906 15.013369665005078 16.03102499319865 2 36.8667409596456 21.847573858070106 28.612157913433258 12.673527268851032 3 43.03486113824271 19.013587202088324 22.289120768576005 15.662430891092965 4 35.67446777251403 20.03975184588232 28.26377346534525 16.022006916258402 5 38.07904762136662 17.62780840852589 28.005558175379587 16.287585794727903 6 34.37294804875416 18.420654638563033 30.509442594334637 16.696954718348174 7 40.094156746281364 19.117374519672627 21.409418434236237 19.379050299809776 8 34.387963563433246 26.604110138664893 20.263991686545314 18.743934611356543 9 34.34600128727602 21.62680520981544 23.420658696810246 20.6065348060983 10-11 28.81013273985526 28.654404266895227 27.873935690850303 14.661527302399207 12-13 28.363331232513172 28.075804300959195 26.940563293057917 16.620301173469713 14-15 27.203851438932713 28.68773600398466 27.18326259808445 16.925149958998176 16-17 27.002819399612086 28.13047102452074 27.374108436906564 17.492601138960605 18-19 25.71968279657603 28.395393402426343 27.979964162971633 17.904959638025993 20-21 25.6604459147925 28.428211094869106 28.207361281670224 17.703981708668174 22-23 25.045185292503497 29.764652297466338 27.25152350291295 17.93863890711722 24-25 24.43162879689712 28.769044006562456 28.12146932397159 18.67785787256884 26-27 24.442701201809328 28.421433822027463 27.592942653990864 19.542922322172345 28-29 24.666459425509114 29.31384238363044 27.363475829214888 18.65622236164556 30-31 23.843636276076605 28.59439631814169 28.288039236216235 19.273928169565473 32-33 23.88700541126683 29.185655881739432 27.48967378997272 19.43766491702102 34-35 23.529013897332298 29.217797199647094 28.337752764545638 18.91543613847497 36-37 23.576224839881856 28.367459134126964 28.356812998944587 19.699503027046596 38-39 23.893251994511036 29.261008229116612 27.607812357960082 19.23792741841227 40-41 23.13264489227653 29.613503357658466 27.86584625141058 19.38800549865442 42-43 23.270638824926404 28.95884044514102 28.175828700844143 19.594692029088435 44-45 22.935475261361653 28.501883638245683 28.303827352166966 20.2588137482257 46-47 23.594373095899755 28.253901947047126 27.71939773311882 20.432327223934298 48-49 22.855338625616973 29.068568180336264 28.10025667783077 19.975836516215995 50-51 23.25001535376841 28.330191785476877 28.352863958036462 20.066928902718253 52-53 22.796209370678316 28.40546471165869 27.67683468680421 21.121491230858787 54-55 22.940895569230683 28.495516201602506 28.544919827330812 20.018668401835995 56-57 22.576804503542792 28.306124135844918 28.437332717175636 20.679738643436657 58-59 22.403099485290525 28.71379991469083 28.23889056413765 20.644210035880988 60-61 22.810675957856326 28.468828646704463 28.44372122318606 20.27677417225316 62-63 22.31633228674741 28.5435365766224 28.18991138064118 20.95021975598901 64-65 22.671012301268114 28.612697683491596 28.31583256293148 20.400457452308814 66-67 22.33373837928048 29.295414863644005 27.711988250691245 20.658858506384277 68-69 22.20544842739022 29.603246615414523 27.546757422832613 20.64454753436264 70-71 22.055464229188402 29.451114177921323 27.768330974150274 20.72509061874001 72-73 22.064320622304894 29.461253065020713 27.61564555677687 20.85878075589752 74-75 21.76369560702924 29.5514087093691 27.90669799010917 20.778197693492494 76-77 21.956042460515068 29.20481434178071 27.845952859558793 20.99319033814543 78-79 21.831662226510336 29.48154675719328 27.76517933492217 20.92161168137422 80-81 21.75662535469781 29.33468592875092 27.90911727592081 20.999571440630458 82-83 21.8705435372819 29.44204241301215 27.538508031406504 21.14890601829945 84-85 21.646654205971583 29.49131874998224 27.789496410527097 21.072530633519072 86-87 21.909203115268504 29.02840223174693 27.744961064554758 21.317433588429807 88-89 21.80253220039529 29.397848211374743 27.517055623722435 21.282563964507528 90-91 21.74621167385228 29.08575157000552 27.751918226728804 21.416118529413396 92-93 21.80602812076805 29.102356835852156 27.528805997004746 21.56280904637505 94-95 21.513802630854457 29.200568796831817 27.766116868511258 21.519511703802472 96-97 21.619882609751496 28.888283094897776 27.74690408078917 21.74493021456156 98-99 21.52953958532118 29.160935648495567 27.623906675958054 21.6856180902252 100-101 21.61221493096545 28.926205584940945 27.677579741676123 21.783999742417482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 6.0 2 49.5 3 113.0 4 80.5 5 29.0 6 26.5 7 30.0 8 35.0 9 30.0 10 28.0 11 27.0 12 33.5 13 47.5 14 53.5 15 74.5 16 94.5 17 144.0 18 236.0 19 388.0 20 639.0 21 1035.5 22 1711.0 23 2727.0 24 4214.5 25 6230.0 26 8982.5 27 12653.5 28 17397.0 29 23121.0 30 30085.5 31 38453.5 32 48133.5 33 59497.5 34 72404.5 35 87385.5 36 103838.5 37 120722.5 38 137170.0 39 151445.0 40 163250.0 41 172457.0 42 179531.0 43 183045.0 44 183449.0 45 183885.5 46 183763.0 47 181498.0 48 176373.5 49 167833.0 50 156825.0 51 143962.0 52 127846.5 53 109532.0 54 91031.5 55 73975.5 56 59672.0 57 48171.0 58 39031.0 59 31406.5 60 25201.0 61 19904.5 62 15370.5 63 11719.5 64 8898.5 65 6836.5 66 5171.5 67 3918.0 68 2994.5 69 2331.0 70 1761.0 71 1353.5 72 1086.0 73 869.5 74 677.0 75 505.0 76 378.0 77 296.5 78 233.0 79 168.0 80 131.0 81 114.0 82 89.5 83 69.0 84 52.5 85 34.5 86 24.5 87 21.0 88 15.0 89 14.0 90 10.0 91 4.5 92 4.0 93 3.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15510620838774766 2 0.0 3 0.005383941299347948 4 0.006709635388132116 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.05824721056378E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0291236052818898E-4 24-25 2.705498140375853E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.928143863240316E-4 40-41 0.0 42-43 0.0 44-45 1.4880239772067192E-4 46-47 0.0011227817282559792 48-49 0.002813718065990887 50-51 4.328797024601365E-4 52-53 0.0016909363377349083 54-55 0.0024890582891457847 56-57 0.0041529396454769345 58-59 0.005640963622683654 60-61 0.0015962439028217536 62-63 8.65759404920273E-4 64-65 0.0014609689958029607 66-67 0.00811649442112756 68-69 0.007210152544101648 70-71 0.009076946260960988 72-73 0.006479668046200168 74-75 0.008914616372538436 76-77 0.004829314180570898 78-79 0.004694039273552105 80-81 0.0019750136424743726 82-83 0.0 84-85 5.816821001808085E-4 86-87 0.0015962439028217536 88-89 0.0049240066154840525 90-91 0.006777272841641512 92-93 0.008982253826047833 94-95 0.008265296818848232 96-97 0.010402640349745156 98-99 0.007588922283754268 100-101 0.007413064904629838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3696177.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.83380222096994 #Duplication Level Percentage of deduplicated Percentage of total 1 96.71518252680865 89.78438126458764 2 2.447871385887252 4.544904161996575 3 0.3917316001931145 1.0909780168809498 4 0.15708674642135653 0.5833183979526348 5 0.06959520804851287 0.32303938897514495 6 0.031262731572722816 0.17413429438256534 7 0.04598723598442549 0.29884189790470594 8 0.03356896187082387 0.24930674936634759 9 0.014935818099657345 0.12478939051247756 >10 0.0868767737737466 1.3907493660959753 >50 0.004523960367315277 0.2652148374520302 >100 0.0012752377367949235 0.20022720867151717 >500 7.268712196179023E-5 0.05019829269424735 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.9126113650571273E-5 0.9199167325271956 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 34022 0.9204645773186727 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010551442747465827 0.0 0.0 2 0.0015150789586104779 0.0 0.0 3 0.0021643985123006824 0.0 0.0 4 0.002651388177568336 0.0 0.0 5 0.003300707731258541 0.0 0.0 6 0.005492161224962982 0.0 0.0 7 0.008332934272357628 0.0 0.0 8 0.010957267468522205 0.0 0.0 9 0.0125264563899402 0.0 2.705498140375853E-5 10-11 0.01690936337734908 0.0 5.410996280751706E-5 12-13 0.02138696279967112 0.0 9.469243491315487E-5 14-15 0.025093495251986037 2.705498140375853E-5 1.0821992561503413E-4 16-17 0.029327599841674247 2.705498140375853E-5 1.0821992561503413E-4 18-19 0.03477917859453159 2.705498140375853E-5 1.3527490701879265E-4 20-21 0.04046072468932088 2.705498140375853E-5 1.4880239772067192E-4 22-23 0.04721094254955864 2.705498140375853E-5 1.623298884225512E-4 24-25 0.05363650063295129 2.705498140375853E-5 1.623298884225512E-4 26-27 0.05911513436721239 2.705498140375853E-5 2.0291236052818898E-4 28-29 0.06486431791551109 2.705498140375853E-5 2.1643985123006825E-4 30-31 0.09327204838945755 2.705498140375853E-5 2.1643985123006825E-4 32-33 0.10267365442726363 2.705498140375853E-5 2.1643985123006825E-4 34-35 0.10893688262223372 2.705498140375853E-5 2.5702232333570606E-4 36-37 0.11634994752686356 4.05824721056378E-5 2.8407730473946455E-4 38-39 0.12481815670623998 5.410996280751706E-5 3.246597768451024E-4 40-41 0.13430092768825735 5.410996280751706E-5 3.5171475824886093E-4 42-43 0.14583987725696035 5.410996280751706E-5 4.0582472105637796E-4 44-45 0.15820400375847804 5.410996280751706E-5 4.328797024601365E-4 46-47 0.1721779016535193 5.410996280751706E-5 4.5993468386389504E-4 48-49 0.18762629603506542 5.410996280751706E-5 4.869896652676536E-4 50-51 0.20461682435662576 5.410996280751706E-5 4.869896652676536E-4 52-53 0.22532741262120293 5.410996280751706E-5 4.869896652676536E-4 54-55 0.24783715714913002 5.410996280751706E-5 5.140446466714121E-4 56-57 0.2728630149476067 5.410996280751706E-5 5.410996280751706E-4 58-59 0.2991875118534637 5.410996280751706E-5 5.681546094789292E-4 60-61 0.3283663092974173 5.410996280751706E-5 5.681546094789292E-4 62-63 0.35965539529086404 5.410996280751706E-5 5.681546094789292E-4 64-65 0.3940693316364449 5.410996280751706E-5 5.681546094789292E-4 66-67 0.4314728434271411 5.410996280751706E-5 5.681546094789292E-4 68-69 0.4719741505885676 6.763745350939633E-5 5.681546094789292E-4 70-71 0.5148833510949287 8.11649442112756E-5 6.08737081584567E-4 72-73 0.5616073039792195 8.11649442112756E-5 6.763745350939633E-4 74-75 0.6115778546319617 8.11649442112756E-5 7.169570071996011E-4 76-77 0.6674599187214248 8.11649442112756E-5 7.845944607089974E-4 78-79 0.7277654722704026 8.11649442112756E-5 7.845944607089974E-4 80-81 0.7917910857623972 8.11649442112756E-5 7.845944607089974E-4 82-83 0.8591174069856503 8.11649442112756E-5 7.845944607089974E-4 84-85 0.9305560853822747 1.0821992561503413E-4 7.845944607089974E-4 86-87 1.0122756567123274 1.0821992561503413E-4 7.981219514108767E-4 88-89 1.0969983309781972 1.0821992561503413E-4 8.522319142183938E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 9290 0.0 42.084084 3 CGGAAGA 9485 0.0 41.115936 4 GATCGGA 9855 0.0 39.976185 1 ATCGGAA 9800 0.0 39.893993 2 ATGCCGT 5020 0.0 38.227005 48-49 GCCGTCT 5045 0.0 38.03757 50-51 CGTCTTC 5125 0.0 37.72161 52-53 CGTATGC 5045 0.0 37.613888 46-47 TGCCGTC 5055 0.0 37.539482 50-51 TCTCGTA 5020 0.0 37.280792 42-43 TATGCCG 5095 0.0 37.244762 48-49 CTCGTAT 5050 0.0 37.059326 44-45 AATCTCG 5095 0.0 36.77787 40-41 TCGTATG 5245 0.0 36.76827 44-45 AGAGCAC 10630 0.0 36.550148 8 GAGCACA 10630 0.0 36.505466 9 CCGTCTT 5255 0.0 36.20091 52-53 ATCTCGT 5180 0.0 35.945866 42-43 GAAGAGC 11070 0.0 35.1832 6 GTATGCC 5545 0.0 34.65051 46-47 >>END_MODULE